Journal: mAbs

Article Title: Development of a nonhuman primate challenge model to evaluate CD8 + T cell responses to an adenovirus-based vaccine expressing SIV proteins upon repeat-dose treatment with checkpoint inhibitors

doi: 10.1080/19420862.2021.1979447

Figure Lengend Snippet: Detection of cellular IFNγ response by ELISPOT assay following ex-vivo stimulation of freshly isolated PBMCs (sixteen immunized MCMs) with a peptide pool covering the complete sequence of the Adenovirus hexon, at 0.6 µM (total peptide concentration, A and C) or with a pool of three Nef peptides (RM9, HW8 and LT9 at 10 µM each, B and D) or Conditions for which spots were too numerous to count were arbitrary set at 3000 SFCs per 10 6 PBMCs. *, p < .05 indicates statistical significance compared to the untreated group

Article Snippet: PBMCs were incubated for 4 hours, in a humidified incubator set to maintain 37°C, 5% CO 2 in the presence of the Nef RM9, LT9, and HW8 peptide pool (10 μM/peptide) (MBL International), PepTivator AdV5 Hexon peptide pool (0.6 μM/peptide) (Miltenyi) or medium only (as negative control).

Techniques: Enzyme-linked Immunospot, Ex Vivo, Isolation, Sequencing, Concentration Assay

Journal: Molecules

Article Title: Bicyclo[3.2.0]carbocyclic Molecules and Redox Biotransformations: The Evolution of Closed-Loop Artificial Linear Biocatalytic Cascades and Related Redox-Neutral Systems

doi: 10.3390/molecules28217249

Figure Lengend Snippet: Screen by Glaxo Group Research of eukarytic microbial strains for the bioreduction of ( rac )-bicyclo[3.2.0]hept-2-en-6-one. Adapted with permission from [ 89 ]. 1983. Royal Society of Chemistry.

Article Snippet: Interesting in this respect is that while S. cerevisiae RM9, like the commercial bakers’ yeast strain studied previously by Glaxo Group Research, bioreduced the unsubstituted ( rac )-bicyclic ketone to principally the (+)-( 1S , 5R , 6S )- endo -alcohol, two of the other tested yeast strains (RM1 and ML31) produced the corresponding (-)-( 1R , 5S , 6R ) enantiomer as the principal bioreduction product.

Techniques:

Journal: Molecules

Article Title: Bicyclo[3.2.0]carbocyclic Molecules and Redox Biotransformations: The Evolution of Closed-Loop Artificial Linear Biocatalytic Cascades and Related Redox-Neutral Systems

doi: 10.3390/molecules28217249

Figure Lengend Snippet: Bioreduction of substituted ( rac )-bicyclo[3.2.0]heptenones by a washed cell suspension of Saccharomyces cerevisiae compared to the biooxidation of equivalent heptenols by a washed cell suspension of Bacillus stearothermophilus . Adapted with permission from [ 124 ]. 1994. Elsevier.

Article Snippet: Interesting in this respect is that while S. cerevisiae RM9, like the commercial bakers’ yeast strain studied previously by Glaxo Group Research, bioreduced the unsubstituted ( rac )-bicyclic ketone to principally the (+)-( 1S , 5R , 6S )- endo -alcohol, two of the other tested yeast strains (RM1 and ML31) produced the corresponding (-)-( 1R , 5S , 6R ) enantiomer as the principal bioreduction product.

Techniques: Suspension

Journal: Molecules

Article Title: Bicyclo[3.2.0]carbocyclic Molecules and Redox Biotransformations: The Evolution of Closed-Loop Artificial Linear Biocatalytic Cascades and Related Redox-Neutral Systems

doi: 10.3390/molecules28217249

Figure Lengend Snippet: Bioreduction by growing cultures of various yeasts and fungi of ( rac )-bicyclo[3.2.0]hept-2-en-6-one (ketone 1), ( rac )-4-methylbicyclo[3.2.0]hept-2-en-6-one (ketone 2), ( rac )-1-methylbicyclo[3.2.0]hept-2-en-6-one (ketone 3), and ( rac )-1,4-dimethylbicyclo[3.2.0]hept-2-en-6-one (ketone 4). Adapted with permission from [ 127 ]. 1996. Elsevier.

Article Snippet: Interesting in this respect is that while S. cerevisiae RM9, like the commercial bakers’ yeast strain studied previously by Glaxo Group Research, bioreduced the unsubstituted ( rac )-bicyclic ketone to principally the (+)-( 1S , 5R , 6S )- endo -alcohol, two of the other tested yeast strains (RM1 and ML31) produced the corresponding (-)-( 1R , 5S , 6R ) enantiomer as the principal bioreduction product.

Techniques: