rm-il3 Search Results


recombinant murine lif  (ProSpec)
90
ProSpec recombinant murine lif
BMP2 Signals Noncanonically through JAK/STAT to Induce GFAP Independently of LIF (A) WB analysis of the effects of the cytokines LIF and BMP2 at 20 ng/ml on GFAP induction and the role of JAK/STAT signaling in non-irr NSCs. (B) Quantitative real-time PCR analysis of GFAP and LIF expression levels in NSCs exposed for 8 hr to 20 ng/ml <t>recombinant</t> BMP2. Error bars show SD. (C) Representative wide-field microscopy images of the effect recombinant cytokines (20 ng/ml) on NSCs after 6 days. GFAP detected by IF analysis, magnification: 40×. (D) Quantification of Nestin and GFAP-positive cells after cytokine exposure for 6 days at 20 ng/ml or 100 ng/ml. (E) Quantitative real-time PCR analysis of wild-type and isogenic BMP2 -deficient NSCs for the expression of BMP2/4 , NSC markers Nestin , SOX2 , PAX6 , and the astrocyte marker GFAP . Error bars show SD. ‡ GFAP , BMP2 , and BMP4 gene expression were not detectable in irr BMP2 −/− NSCs. (F) Quantitative real-time PCR analysis of BMP2 -deficient NSCs for the expression of Nestin and GFAP . Error bars show SD. ‡As in (E), GFAP not detectable.
Recombinant Murine Lif, supplied by ProSpec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/recombinant murine lif/product/ProSpec
Average 90 stars, based on 1 article reviews
recombinant murine lif - by Bioz Stars, 2026-06
90/100 stars
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cytokines rm-il-3  (ProSpec)
90
ProSpec cytokines rm-il-3
BMP2 Signals Noncanonically through JAK/STAT to Induce GFAP Independently of LIF (A) WB analysis of the effects of the cytokines LIF and BMP2 at 20 ng/ml on GFAP induction and the role of JAK/STAT signaling in non-irr NSCs. (B) Quantitative real-time PCR analysis of GFAP and LIF expression levels in NSCs exposed for 8 hr to 20 ng/ml <t>recombinant</t> BMP2. Error bars show SD. (C) Representative wide-field microscopy images of the effect recombinant cytokines (20 ng/ml) on NSCs after 6 days. GFAP detected by IF analysis, magnification: 40×. (D) Quantification of Nestin and GFAP-positive cells after cytokine exposure for 6 days at 20 ng/ml or 100 ng/ml. (E) Quantitative real-time PCR analysis of wild-type and isogenic BMP2 -deficient NSCs for the expression of BMP2/4 , NSC markers Nestin , SOX2 , PAX6 , and the astrocyte marker GFAP . Error bars show SD. ‡ GFAP , BMP2 , and BMP4 gene expression were not detectable in irr BMP2 −/− NSCs. (F) Quantitative real-time PCR analysis of BMP2 -deficient NSCs for the expression of Nestin and GFAP . Error bars show SD. ‡As in (E), GFAP not detectable.
Cytokines Rm Il 3, supplied by ProSpec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cytokines rm-il-3/product/ProSpec
Average 90 stars, based on 1 article reviews
cytokines rm-il-3 - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
rm-il3  (ProSpec)
90
ProSpec rm-il3
BMP2 Signals Noncanonically through JAK/STAT to Induce GFAP Independently of LIF (A) WB analysis of the effects of the cytokines LIF and BMP2 at 20 ng/ml on GFAP induction and the role of JAK/STAT signaling in non-irr NSCs. (B) Quantitative real-time PCR analysis of GFAP and LIF expression levels in NSCs exposed for 8 hr to 20 ng/ml <t>recombinant</t> BMP2. Error bars show SD. (C) Representative wide-field microscopy images of the effect recombinant cytokines (20 ng/ml) on NSCs after 6 days. GFAP detected by IF analysis, magnification: 40×. (D) Quantification of Nestin and GFAP-positive cells after cytokine exposure for 6 days at 20 ng/ml or 100 ng/ml. (E) Quantitative real-time PCR analysis of wild-type and isogenic BMP2 -deficient NSCs for the expression of BMP2/4 , NSC markers Nestin , SOX2 , PAX6 , and the astrocyte marker GFAP . Error bars show SD. ‡ GFAP , BMP2 , and BMP4 gene expression were not detectable in irr BMP2 −/− NSCs. (F) Quantitative real-time PCR analysis of BMP2 -deficient NSCs for the expression of Nestin and GFAP . Error bars show SD. ‡As in (E), GFAP not detectable.
Rm Il3, supplied by ProSpec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rm-il3/product/ProSpec
Average 90 stars, based on 1 article reviews
rm-il3 - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier

Image Search Results


BMP2 Signals Noncanonically through JAK/STAT to Induce GFAP Independently of LIF (A) WB analysis of the effects of the cytokines LIF and BMP2 at 20 ng/ml on GFAP induction and the role of JAK/STAT signaling in non-irr NSCs. (B) Quantitative real-time PCR analysis of GFAP and LIF expression levels in NSCs exposed for 8 hr to 20 ng/ml recombinant BMP2. Error bars show SD. (C) Representative wide-field microscopy images of the effect recombinant cytokines (20 ng/ml) on NSCs after 6 days. GFAP detected by IF analysis, magnification: 40×. (D) Quantification of Nestin and GFAP-positive cells after cytokine exposure for 6 days at 20 ng/ml or 100 ng/ml. (E) Quantitative real-time PCR analysis of wild-type and isogenic BMP2 -deficient NSCs for the expression of BMP2/4 , NSC markers Nestin , SOX2 , PAX6 , and the astrocyte marker GFAP . Error bars show SD. ‡ GFAP , BMP2 , and BMP4 gene expression were not detectable in irr BMP2 −/− NSCs. (F) Quantitative real-time PCR analysis of BMP2 -deficient NSCs for the expression of Nestin and GFAP . Error bars show SD. ‡As in (E), GFAP not detectable.

Journal: Stem Cell Reports

Article Title: DNA Damage in Mammalian Neural Stem Cells Leads to Astrocytic Differentiation Mediated by BMP2 Signaling through JAK-STAT

doi: 10.1016/j.stemcr.2013.06.004

Figure Lengend Snippet: BMP2 Signals Noncanonically through JAK/STAT to Induce GFAP Independently of LIF (A) WB analysis of the effects of the cytokines LIF and BMP2 at 20 ng/ml on GFAP induction and the role of JAK/STAT signaling in non-irr NSCs. (B) Quantitative real-time PCR analysis of GFAP and LIF expression levels in NSCs exposed for 8 hr to 20 ng/ml recombinant BMP2. Error bars show SD. (C) Representative wide-field microscopy images of the effect recombinant cytokines (20 ng/ml) on NSCs after 6 days. GFAP detected by IF analysis, magnification: 40×. (D) Quantification of Nestin and GFAP-positive cells after cytokine exposure for 6 days at 20 ng/ml or 100 ng/ml. (E) Quantitative real-time PCR analysis of wild-type and isogenic BMP2 -deficient NSCs for the expression of BMP2/4 , NSC markers Nestin , SOX2 , PAX6 , and the astrocyte marker GFAP . Error bars show SD. ‡ GFAP , BMP2 , and BMP4 gene expression were not detectable in irr BMP2 −/− NSCs. (F) Quantitative real-time PCR analysis of BMP2 -deficient NSCs for the expression of Nestin and GFAP . Error bars show SD. ‡As in (E), GFAP not detectable.

Article Snippet: Recombinant murine Noggin, LIF, IL-6, and human BMP2 (Prospec) were applied at 200 ng/ml (Noggin) and 20 ng/ml (unless stated otherwise).

Techniques: Real-time Polymerase Chain Reaction, Expressing, Recombinant, Microscopy, Marker, Gene Expression