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Image Search Results
Journal: PLoS ONE
Article Title: microRNA-153 Targets mTORC2 Component Rictor to Inhibit Glioma Cells
doi: 10.1371/journal.pone.0156915
Figure Lengend Snippet: Relative Rictor mRNA (A) and miRNA-153 (C) expression in stable U87MG cells with scramble control shRNA (“shRNA-C”) or Rictor shRNA (“shRNA-Rictor”) was tested by Real-time PCR assay. Expressions of listed proteins in these cells were also shown (B). Same number of U87MG cells with “shRNA-C” or “shRNA-Rictor” were subjected to MTT assay (D) and clonogenicity assay (E) to test cell growth; Cell apoptosis was also tested (Histone DNA ELISA assay, F). Rictor shRNA-expressing stable U87MG cells were transfected with miR-153 or microRNA-control (“miR-C”), miR-153 expression (G, Real-time PCR assay), cell growth (E, MTT assay) and apoptosis (F, Histone DNA ELISA assay) in these cells were tested. Rictor expression (vs. Tubulin) and p-Akt Ser473 (vs. Akt1) were quantified (B). Bars stand for mean ± SD. * p < 0.05 vs. “shRNA-C” group (A, C, D-F, n = 5). * p < 0.05 vs. “miR-C” group (G, n = 5).
Article Snippet: The
Techniques: Expressing, shRNA, Real-time Polymerase Chain Reaction, MTT Assay, Enzyme-linked Immunosorbent Assay, Transfection
Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology
Article Title: Rictor Deficiency Aggravates Hepatic Ischemia/Reperfusion Injury in Mice by Suppressing Autophagy and Regulating MAPK Signaling.
doi: 10.1159/000488165
Figure Lengend Snippet: Fig. 7. Rictor deficiency affects MAPKs activation on LPS treat ment RAW264.7 cell line. (A) Western blot analysis of Rictor, P-JNK, JNK, P-ERK and ERK in control and Rictor siRNA pretreat ment of RAW264.7 at the indicated time points, upon LPS stimuli. (B) Supernatant cytokine levels of IL-6 and TNF-a after in LPS-stimulated at the indicated time points, mea sured by ELISA. (C) mRNA levels of cytokines and chemokines such as IL-6, TNF-a, IL-1β and IL-10 were determined by quantitative real- time PCR. n=3-5 replicates per ex periment group performed on dif ferent days. All data shown as the mean ± SEM, **P<0.01, *P<0.05.
Article Snippet: RICTOR-targeting
Techniques: Activation Assay, Western Blot, Control, Enzyme-linked Immunosorbent Assay, Real-time Polymerase Chain Reaction