rictor shrna Search Results


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Addgene inc rictor 2 shrna
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Santa Cruz Biotechnology rictor shrna lentiviral particles
Relative <t>Rictor</t> mRNA (A) and miRNA-153 (C) expression in stable U87MG cells with scramble control <t>shRNA</t> (“shRNA-C”) or Rictor shRNA (“shRNA-Rictor”) was tested by Real-time PCR assay. Expressions of listed proteins in these cells were also shown (B). Same number of U87MG cells with “shRNA-C” or “shRNA-Rictor” were subjected to MTT assay (D) and clonogenicity assay (E) to test cell growth; Cell apoptosis was also tested (Histone DNA ELISA assay, F). Rictor shRNA-expressing stable U87MG cells were transfected with miR-153 or microRNA-control (“miR-C”), miR-153 expression (G, Real-time PCR assay), cell growth (E, MTT assay) and apoptosis (F, Histone DNA ELISA assay) in these cells were tested. Rictor expression (vs. Tubulin) and p-Akt Ser473 (vs. Akt1) were quantified (B). Bars stand for mean ± SD. * p < 0.05 vs. “shRNA-C” group (A, C, D-F, n = 5). * p < 0.05 vs. “miR-C” group (G, n = 5).
Rictor Shrna Lentiviral Particles, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cell Signaling Technology Inc kinase 1 ask1
Relative <t>Rictor</t> mRNA (A) and miRNA-153 (C) expression in stable U87MG cells with scramble control <t>shRNA</t> (“shRNA-C”) or Rictor shRNA (“shRNA-Rictor”) was tested by Real-time PCR assay. Expressions of listed proteins in these cells were also shown (B). Same number of U87MG cells with “shRNA-C” or “shRNA-Rictor” were subjected to MTT assay (D) and clonogenicity assay (E) to test cell growth; Cell apoptosis was also tested (Histone DNA ELISA assay, F). Rictor shRNA-expressing stable U87MG cells were transfected with miR-153 or microRNA-control (“miR-C”), miR-153 expression (G, Real-time PCR assay), cell growth (E, MTT assay) and apoptosis (F, Histone DNA ELISA assay) in these cells were tested. Rictor expression (vs. Tubulin) and p-Akt Ser473 (vs. Akt1) were quantified (B). Bars stand for mean ± SD. * p < 0.05 vs. “shRNA-C” group (A, C, D-F, n = 5). * p < 0.05 vs. “miR-C” group (G, n = 5).
Kinase 1 Ask1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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OriGene rictor
Relative <t>Rictor</t> mRNA (A) and miRNA-153 (C) expression in stable U87MG cells with scramble control <t>shRNA</t> (“shRNA-C”) or Rictor shRNA (“shRNA-Rictor”) was tested by Real-time PCR assay. Expressions of listed proteins in these cells were also shown (B). Same number of U87MG cells with “shRNA-C” or “shRNA-Rictor” were subjected to MTT assay (D) and clonogenicity assay (E) to test cell growth; Cell apoptosis was also tested (Histone DNA ELISA assay, F). Rictor shRNA-expressing stable U87MG cells were transfected with miR-153 or microRNA-control (“miR-C”), miR-153 expression (G, Real-time PCR assay), cell growth (E, MTT assay) and apoptosis (F, Histone DNA ELISA assay) in these cells were tested. Rictor expression (vs. Tubulin) and p-Akt Ser473 (vs. Akt1) were quantified (B). Bars stand for mean ± SD. * p < 0.05 vs. “shRNA-C” group (A, C, D-F, n = 5). * p < 0.05 vs. “miR-C” group (G, n = 5).
Rictor, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Santa Cruz Biotechnology shrna plasmids
Fig. <t>7.</t> <t>Rictor</t> deficiency affects MAPKs activation on LPS treat ment RAW264.7 cell line. (A) Western blot analysis of Rictor, P-JNK, JNK, P-ERK and ERK in control and Rictor <t>siRNA</t> pretreat ment of RAW264.7 at the indicated time points, upon LPS stimuli. (B) Supernatant cytokine levels of IL-6 and TNF-a after in LPS-stimulated at the indicated time points, mea sured by ELISA. (C) mRNA levels of cytokines and chemokines such as IL-6, TNF-a, IL-1β and IL-10 were determined by quantitative real- time PCR. n=3-5 replicates per ex periment group performed on dif ferent days. All data shown as the mean ± SEM, **P<0.01, *P<0.05.
Shrna Plasmids, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Addgene inc rictor short hairpin rnas shrnas
Fig. <t>7.</t> <t>Rictor</t> deficiency affects MAPKs activation on LPS treat ment RAW264.7 cell line. (A) Western blot analysis of Rictor, P-JNK, JNK, P-ERK and ERK in control and Rictor <t>siRNA</t> pretreat ment of RAW264.7 at the indicated time points, upon LPS stimuli. (B) Supernatant cytokine levels of IL-6 and TNF-a after in LPS-stimulated at the indicated time points, mea sured by ELISA. (C) mRNA levels of cytokines and chemokines such as IL-6, TNF-a, IL-1β and IL-10 were determined by quantitative real- time PCR. n=3-5 replicates per ex periment group performed on dif ferent days. All data shown as the mean ± SEM, **P<0.01, *P<0.05.
Rictor Short Hairpin Rnas Shrnas, supplied by Addgene inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Relative Rictor mRNA (A) and miRNA-153 (C) expression in stable U87MG cells with scramble control shRNA (“shRNA-C”) or Rictor shRNA (“shRNA-Rictor”) was tested by Real-time PCR assay. Expressions of listed proteins in these cells were also shown (B). Same number of U87MG cells with “shRNA-C” or “shRNA-Rictor” were subjected to MTT assay (D) and clonogenicity assay (E) to test cell growth; Cell apoptosis was also tested (Histone DNA ELISA assay, F). Rictor shRNA-expressing stable U87MG cells were transfected with miR-153 or microRNA-control (“miR-C”), miR-153 expression (G, Real-time PCR assay), cell growth (E, MTT assay) and apoptosis (F, Histone DNA ELISA assay) in these cells were tested. Rictor expression (vs. Tubulin) and p-Akt Ser473 (vs. Akt1) were quantified (B). Bars stand for mean ± SD. * p < 0.05 vs. “shRNA-C” group (A, C, D-F, n = 5). * p < 0.05 vs. “miR-C” group (G, n = 5).

Journal: PLoS ONE

Article Title: microRNA-153 Targets mTORC2 Component Rictor to Inhibit Glioma Cells

doi: 10.1371/journal.pone.0156915

Figure Lengend Snippet: Relative Rictor mRNA (A) and miRNA-153 (C) expression in stable U87MG cells with scramble control shRNA (“shRNA-C”) or Rictor shRNA (“shRNA-Rictor”) was tested by Real-time PCR assay. Expressions of listed proteins in these cells were also shown (B). Same number of U87MG cells with “shRNA-C” or “shRNA-Rictor” were subjected to MTT assay (D) and clonogenicity assay (E) to test cell growth; Cell apoptosis was also tested (Histone DNA ELISA assay, F). Rictor shRNA-expressing stable U87MG cells were transfected with miR-153 or microRNA-control (“miR-C”), miR-153 expression (G, Real-time PCR assay), cell growth (E, MTT assay) and apoptosis (F, Histone DNA ELISA assay) in these cells were tested. Rictor expression (vs. Tubulin) and p-Akt Ser473 (vs. Akt1) were quantified (B). Bars stand for mean ± SD. * p < 0.05 vs. “shRNA-C” group (A, C, D-F, n = 5). * p < 0.05 vs. “miR-C” group (G, n = 5).

Article Snippet: The Rictor shRNA lentiviral particles were purchased from Santa Cruz Biotech (sc-61478-V, Santa Cruz, CA).

Techniques: Expressing, shRNA, Real-time Polymerase Chain Reaction, MTT Assay, Enzyme-linked Immunosorbent Assay, Transfection

Fig. 7. Rictor deficiency affects MAPKs activation on LPS treat ment RAW264.7 cell line. (A) Western blot analysis of Rictor, P-JNK, JNK, P-ERK and ERK in control and Rictor siRNA pretreat ment of RAW264.7 at the indicated time points, upon LPS stimuli. (B) Supernatant cytokine levels of IL-6 and TNF-a after in LPS-stimulated at the indicated time points, mea sured by ELISA. (C) mRNA levels of cytokines and chemokines such as IL-6, TNF-a, IL-1β and IL-10 were determined by quantitative real- time PCR. n=3-5 replicates per ex periment group performed on dif ferent days. All data shown as the mean ± SEM, **P<0.01, *P<0.05.

Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Article Title: Rictor Deficiency Aggravates Hepatic Ischemia/Reperfusion Injury in Mice by Suppressing Autophagy and Regulating MAPK Signaling.

doi: 10.1159/000488165

Figure Lengend Snippet: Fig. 7. Rictor deficiency affects MAPKs activation on LPS treat ment RAW264.7 cell line. (A) Western blot analysis of Rictor, P-JNK, JNK, P-ERK and ERK in control and Rictor siRNA pretreat ment of RAW264.7 at the indicated time points, upon LPS stimuli. (B) Supernatant cytokine levels of IL-6 and TNF-a after in LPS-stimulated at the indicated time points, mea sured by ELISA. (C) mRNA levels of cytokines and chemokines such as IL-6, TNF-a, IL-1β and IL-10 were determined by quantitative real- time PCR. n=3-5 replicates per ex periment group performed on dif ferent days. All data shown as the mean ± SEM, **P<0.01, *P<0.05.

Article Snippet: RICTOR-targeting shRNA plasmids (sc-61479-SH) and control shRNA plasmids (sc-108060) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).

Techniques: Activation Assay, Western Blot, Control, Enzyme-linked Immunosorbent Assay, Real-time Polymerase Chain Reaction