rhoa rhoa gtp Search Results


86
Cytoskeleton Inc rhoa rhoa gtp
Rhoa Rhoa Gtp, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rhoa rhoa gtp/product/Cytoskeleton Inc
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
rhoa rhoa gtp - by Bioz Stars, 2024-11
86/100 stars
  Buy from Supplier

86
Abcam rhoa rhoa gtp
Hydroxyfasudil improves the tendency towards cognitive impairments induced by propofol. Evaluation of rat learning and memory in the Morris water maze. A. Mean escape latency to the platform in the Morris water maze. B. Time spent in platform quadrant among the different groups. C and D. Pull-down experiments for <t>GTP-bound</t> <t>RhoA</t> in primary culture hippocampal neurons with indicated treatment. Data were shown as the mean ± SD, **P < 0.01 vs. the control group; #P < 0.05, ##P < 0.01 vs. the “P” group.
Rhoa Rhoa Gtp, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rhoa rhoa gtp/product/Abcam
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
rhoa rhoa gtp - by Bioz Stars, 2024-11
86/100 stars
  Buy from Supplier

86
Upstate Biotechnology Inc rhoa gtp rhoa
Hydroxyfasudil improves the tendency towards cognitive impairments induced by propofol. Evaluation of rat learning and memory in the Morris water maze. A. Mean escape latency to the platform in the Morris water maze. B. Time spent in platform quadrant among the different groups. C and D. Pull-down experiments for <t>GTP-bound</t> <t>RhoA</t> in primary culture hippocampal neurons with indicated treatment. Data were shown as the mean ± SD, **P < 0.01 vs. the control group; #P < 0.05, ##P < 0.01 vs. the “P” group.
Rhoa Gtp Rhoa, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rhoa gtp rhoa/product/Upstate Biotechnology Inc
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
rhoa gtp rhoa - by Bioz Stars, 2024-11
86/100 stars
  Buy from Supplier

86
NewEast Biosciences gtp rhoa
A Primary cortical microglia expressing the <t>Raichu-RhoA</t> biosensor exposed to 1 µg/ml LPS ( n = 6 cells pooled across 3 independent cultures). Panels represent time-lapse FRET/CFP images coded according to the pseudocolor ramp. Graph (mean and SD) displays FRET/CFP ratio changes normalized at 0 min. * p < 0.05 (One-way ANOVA). B Primary cortical microglia expressing the Raichu-RhoA biosensor were exposed to different concentrations of LPS for 20 min ( n = 10 cells pooled across 5 independent cultures). Graph (mean and SD) displays FRET/CFP ratio changes. The blue line shows untreated cells’ mean FRET/Donor ratio changes. The dashed line indicates the IC 50 . C , Primary cortical microglia treated and non-treated (CT) with LPS (1 µg/ml) for 1 h ( n = 10 independent cultures) and immunostained for <t>GTP-RhoA.</t> Graph (mean and SD) shows amounts of GTP-RhoA as mean fluorescent intensity (MFI). **** p < 0.0001 (Mann-Whitney test). D RhoA pull-down on lysates from primary cortical microglia treated and non-treated (CT) with LPS (1 µg/ml) for 1 h ( n = 3 independent cultures). Graph displays mean with SD. * *p < 0.01 (Mann-Whitney test). E HMC3 microglia expressing Raichu-RhoA biosensor treated and non-treated (CT) with 1 µg/ml LPS for 1 h ( n = 100 cells per group from 10 independent experiments). Pseudocolor ramp represents min/max FRET/CFP ratios. Graph (mean and SD) displays FRET/CFP ratio changes. **** p < 0.0001 (unpaired t-test). F RhoA pull-down on lysates from HCM3 microglia treated and non-treated (CT) with LPS (1 µg/ml) for 1 h ( n = 3 independent cultures). Graph displays mean with SD. * p < 0.05 (Mann-Whitney test). G Immunofluorescence images of GTP-RhoA and Iba-1 on cortical tissue sections from brain cortex of adult mice injected with saline or LPS (4 mg/Kg; 24 h). Graph (mean and SD) shows amounts of GTP-RhoA in Iba-1+ cells ( n = 3 mice per group). * p < 0.001 (paired t-test). Scale bars: 10 µm ( A, C , and G ); 20 µm ( E ).
Gtp Rhoa, supplied by NewEast Biosciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gtp rhoa/product/NewEast Biosciences
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
gtp rhoa - by Bioz Stars, 2024-11
86/100 stars
  Buy from Supplier

86
Cell Signaling Technology Inc rhoa gtp
A Primary cortical microglia expressing the <t>Raichu-RhoA</t> biosensor exposed to 1 µg/ml LPS ( n = 6 cells pooled across 3 independent cultures). Panels represent time-lapse FRET/CFP images coded according to the pseudocolor ramp. Graph (mean and SD) displays FRET/CFP ratio changes normalized at 0 min. * p < 0.05 (One-way ANOVA). B Primary cortical microglia expressing the Raichu-RhoA biosensor were exposed to different concentrations of LPS for 20 min ( n = 10 cells pooled across 5 independent cultures). Graph (mean and SD) displays FRET/CFP ratio changes. The blue line shows untreated cells’ mean FRET/Donor ratio changes. The dashed line indicates the IC 50 . C , Primary cortical microglia treated and non-treated (CT) with LPS (1 µg/ml) for 1 h ( n = 10 independent cultures) and immunostained for <t>GTP-RhoA.</t> Graph (mean and SD) shows amounts of GTP-RhoA as mean fluorescent intensity (MFI). **** p < 0.0001 (Mann-Whitney test). D RhoA pull-down on lysates from primary cortical microglia treated and non-treated (CT) with LPS (1 µg/ml) for 1 h ( n = 3 independent cultures). Graph displays mean with SD. * *p < 0.01 (Mann-Whitney test). E HMC3 microglia expressing Raichu-RhoA biosensor treated and non-treated (CT) with 1 µg/ml LPS for 1 h ( n = 100 cells per group from 10 independent experiments). Pseudocolor ramp represents min/max FRET/CFP ratios. Graph (mean and SD) displays FRET/CFP ratio changes. **** p < 0.0001 (unpaired t-test). F RhoA pull-down on lysates from HCM3 microglia treated and non-treated (CT) with LPS (1 µg/ml) for 1 h ( n = 3 independent cultures). Graph displays mean with SD. * p < 0.05 (Mann-Whitney test). G Immunofluorescence images of GTP-RhoA and Iba-1 on cortical tissue sections from brain cortex of adult mice injected with saline or LPS (4 mg/Kg; 24 h). Graph (mean and SD) shows amounts of GTP-RhoA in Iba-1+ cells ( n = 3 mice per group). * p < 0.001 (paired t-test). Scale bars: 10 µm ( A, C , and G ); 20 µm ( E ).
Rhoa Gtp, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rhoa gtp/product/Cell Signaling Technology Inc
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
rhoa gtp - by Bioz Stars, 2024-11
86/100 stars
  Buy from Supplier

Image Search Results


Hydroxyfasudil improves the tendency towards cognitive impairments induced by propofol. Evaluation of rat learning and memory in the Morris water maze. A. Mean escape latency to the platform in the Morris water maze. B. Time spent in platform quadrant among the different groups. C and D. Pull-down experiments for GTP-bound RhoA in primary culture hippocampal neurons with indicated treatment. Data were shown as the mean ± SD, **P < 0.01 vs. the control group; #P < 0.05, ##P < 0.01 vs. the “P” group.

Journal: International Journal of Clinical and Experimental Pathology

Article Title: The protective effect of the Rho-kinase inhibitor hydroxyfasudil on propofol-induced hippocampal neuron apoptosis in neonatal rats

doi:

Figure Lengend Snippet: Hydroxyfasudil improves the tendency towards cognitive impairments induced by propofol. Evaluation of rat learning and memory in the Morris water maze. A. Mean escape latency to the platform in the Morris water maze. B. Time spent in platform quadrant among the different groups. C and D. Pull-down experiments for GTP-bound RhoA in primary culture hippocampal neurons with indicated treatment. Data were shown as the mean ± SD, **P < 0.01 vs. the control group; #P < 0.05, ##P < 0.01 vs. the “P” group.

Article Snippet: RhoA activation assay The RhoA activation assay was performed to detect the endogenous active form of RhoA (RhoA-GTP) using a RhoA activation assay kit (Abcam; ab211164) following the manufacturer’s manuals.

Techniques:

A Primary cortical microglia expressing the Raichu-RhoA biosensor exposed to 1 µg/ml LPS ( n = 6 cells pooled across 3 independent cultures). Panels represent time-lapse FRET/CFP images coded according to the pseudocolor ramp. Graph (mean and SD) displays FRET/CFP ratio changes normalized at 0 min. * p < 0.05 (One-way ANOVA). B Primary cortical microglia expressing the Raichu-RhoA biosensor were exposed to different concentrations of LPS for 20 min ( n = 10 cells pooled across 5 independent cultures). Graph (mean and SD) displays FRET/CFP ratio changes. The blue line shows untreated cells’ mean FRET/Donor ratio changes. The dashed line indicates the IC 50 . C , Primary cortical microglia treated and non-treated (CT) with LPS (1 µg/ml) for 1 h ( n = 10 independent cultures) and immunostained for GTP-RhoA. Graph (mean and SD) shows amounts of GTP-RhoA as mean fluorescent intensity (MFI). **** p < 0.0001 (Mann-Whitney test). D RhoA pull-down on lysates from primary cortical microglia treated and non-treated (CT) with LPS (1 µg/ml) for 1 h ( n = 3 independent cultures). Graph displays mean with SD. * *p < 0.01 (Mann-Whitney test). E HMC3 microglia expressing Raichu-RhoA biosensor treated and non-treated (CT) with 1 µg/ml LPS for 1 h ( n = 100 cells per group from 10 independent experiments). Pseudocolor ramp represents min/max FRET/CFP ratios. Graph (mean and SD) displays FRET/CFP ratio changes. **** p < 0.0001 (unpaired t-test). F RhoA pull-down on lysates from HCM3 microglia treated and non-treated (CT) with LPS (1 µg/ml) for 1 h ( n = 3 independent cultures). Graph displays mean with SD. * p < 0.05 (Mann-Whitney test). G Immunofluorescence images of GTP-RhoA and Iba-1 on cortical tissue sections from brain cortex of adult mice injected with saline or LPS (4 mg/Kg; 24 h). Graph (mean and SD) shows amounts of GTP-RhoA in Iba-1+ cells ( n = 3 mice per group). * p < 0.001 (paired t-test). Scale bars: 10 µm ( A, C , and G ); 20 µm ( E ).

Journal: Cell Death & Disease

Article Title: RhoA balances microglial reactivity and survival during neuroinflammation

doi: 10.1038/s41419-023-06217-w

Figure Lengend Snippet: A Primary cortical microglia expressing the Raichu-RhoA biosensor exposed to 1 µg/ml LPS ( n = 6 cells pooled across 3 independent cultures). Panels represent time-lapse FRET/CFP images coded according to the pseudocolor ramp. Graph (mean and SD) displays FRET/CFP ratio changes normalized at 0 min. * p < 0.05 (One-way ANOVA). B Primary cortical microglia expressing the Raichu-RhoA biosensor were exposed to different concentrations of LPS for 20 min ( n = 10 cells pooled across 5 independent cultures). Graph (mean and SD) displays FRET/CFP ratio changes. The blue line shows untreated cells’ mean FRET/Donor ratio changes. The dashed line indicates the IC 50 . C , Primary cortical microglia treated and non-treated (CT) with LPS (1 µg/ml) for 1 h ( n = 10 independent cultures) and immunostained for GTP-RhoA. Graph (mean and SD) shows amounts of GTP-RhoA as mean fluorescent intensity (MFI). **** p < 0.0001 (Mann-Whitney test). D RhoA pull-down on lysates from primary cortical microglia treated and non-treated (CT) with LPS (1 µg/ml) for 1 h ( n = 3 independent cultures). Graph displays mean with SD. * *p < 0.01 (Mann-Whitney test). E HMC3 microglia expressing Raichu-RhoA biosensor treated and non-treated (CT) with 1 µg/ml LPS for 1 h ( n = 100 cells per group from 10 independent experiments). Pseudocolor ramp represents min/max FRET/CFP ratios. Graph (mean and SD) displays FRET/CFP ratio changes. **** p < 0.0001 (unpaired t-test). F RhoA pull-down on lysates from HCM3 microglia treated and non-treated (CT) with LPS (1 µg/ml) for 1 h ( n = 3 independent cultures). Graph displays mean with SD. * p < 0.05 (Mann-Whitney test). G Immunofluorescence images of GTP-RhoA and Iba-1 on cortical tissue sections from brain cortex of adult mice injected with saline or LPS (4 mg/Kg; 24 h). Graph (mean and SD) shows amounts of GTP-RhoA in Iba-1+ cells ( n = 3 mice per group). * p < 0.001 (paired t-test). Scale bars: 10 µm ( A, C , and G ); 20 µm ( E ).

Article Snippet: GTP-RhoA (NewEast Biosciences Cat# 26904, RRID:AB_1961799), RhoA (Abcam Cat# ab68826, RRID:AB_1142593), RhoA (Cell Signaling Technology Cat# 2117, RRID:AB_10693922), Iba-1 (FUJIFILM Wako Shibayagi Cat# 27030, RRID:AB_2314667), phosphorylated Src family (Cell Signaling Technology Cat# 6943, RRID:AB_10013641), Src (Cell Signaling Technology Cat# 2102, RRID:AB_331358), phosphorylated ERK 1/2 (Cell Signaling Technology Cat# 9101, RRID:AB_331646), ERK 1/2 (Cell Signaling Technology Cat# 9102, RRID:AB_330744).

Techniques: Expressing, MANN-WHITNEY, Immunofluorescence, Injection, Saline