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Image Search Results
Journal: Calcified Tissue International
Article Title: Regulation of CYP27B1 mRNA Expression in Primary Human Osteoblasts
doi: 10.1007/s00223-016-0131-9
Figure Lengend Snippet: Effects of PTH1-34, rhFGF23, and calcitonin on mRNA levels of CYP27B1 and VDR in primary human osteoblasts. CYP27B1 and VDR mRNA levels were determined after 24 h incubation of primary human osteoblasts in medium supplemented with increasing concentrations of PTH1-34 ( a and b , respectively), rhFGF23 ( c and d , respectively), or calcitonin ( e and f , respectively). Results (mean ± SEM) are expressed as treatment versus control ratios (control was set at 1.0; dashed line ) using cells from 4 to 6 different donors
Article Snippet: Cells were allowed to attach to the well for 24 h. Subsequently, primary human osteoblasts were incubated in medium supplemented with human PTH fragment 1-34 (PTH1-34; Sigma-Aldrich, St. Louis, MO, USA),
Techniques: Incubation, Control
Journal: Calcified Tissue International
Article Title: Regulation of CYP27B1 mRNA Expression in Primary Human Osteoblasts
doi: 10.1007/s00223-016-0131-9
Figure Lengend Snippet: Effects of calcium on differentiation markers in primary human osteoblasts. Osterix ( a ), RUNX2 ( b ), COL1α1 ( c ), ALP ( d ), osteopontin ( e ), osteocalcin ( f ), FGF23 ( g ), DMP1 ( h ), and SOST ( i ) mRNA levels were determined after 24 h incubation of primary human osteoblasts in medium supplemented with increasing concentrations of calcium. Results (mean ± SEM) are expressed as treatment versus control ratios (control was set at 1.0; dashed line ) using cells from 5 different donors. (* p < 0.05)
Article Snippet: Cells were allowed to attach to the well for 24 h. Subsequently, primary human osteoblasts were incubated in medium supplemented with human PTH fragment 1-34 (PTH1-34; Sigma-Aldrich, St. Louis, MO, USA),
Techniques: Incubation, Control