rhctgf Search Results


rhctgf  (EMP Genetech)
90
EMP Genetech rhctgf
(A) HDMECs were transduced <t>with</t> <t>adenoviruses</t> Go, CTGF,CT and NT of CTGF at MOI10 for 24hrs; 25 μg of total proteins were separated via SDS-PAGE for MMP1, MMP9 and transferred to a nitrocellulose membrane. The blots were probed overnight with primary Abs at 4°C. As a control for equal protein loading, membranes were stripped and reprobed for β-actin. (B) Graphical representation of MMP1 and MMP9 protein expression determined by Western blot analysis presented in (A). The values represent the mean ± SEM of three independent experiment done in duplicate. *Significant values at p<0.05 (C) HDMECs were transduced with non silencing (NS) or AdCTGF siRNA with addition of S1P. Blocking endogenous CTGF inhibited S1P stimulated cells migration. (D) HDMECs were transduced with increasing doses of AdN-terminal (AdNT) and AdC-terminal (AdCT) domain of CTGF and expression level was determined by Western Blot analysis. (E) Graphical presentation of AdNT and AdCT expression level presented in (D). (F) HDMECs were cultured to 80% confluence in full medium followed by transduction with adenoviruses (AdGo, AdCTGF, AdNT and AdCT) or treated with <t>rhCTGF.</t> After 8 hrs cells were mechanically “wounded” by scraping with a Fisher-brand ready-tip. For the next 24-72 hrs cells were kept in EBM2 medium containing 0.5%serum. Representative images are presented. (G) The bar graph represents migration rate expressed as percentage of cells that crossed into the “scratch” area in comparison to identical “nonscratch” area. Cell counting was performed 48 hr after injury. The experiments were repeated three times in duplicate. (H) Graphical presentation of the transwell co-culture migration assay. Briefly, dermal fibroblasts were transduced with indicated adenoviruses. Next day, the medium was changed and HDMECs were plated on the cell culture inserts. 24 hr later the inserts were removed and cells were counted as described in the Methods section. (I) Bar graph presents endothelial cell proliferation increased after cells transduction with AdCTGF and AdCT. The values represent the mean ± SEM of three independent experiment done in duplicate.
Rhctgf, supplied by EMP Genetech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rhctgf/rhctgf/pmc03058265-108-15-16
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rhctgf  (FibroGen Inc)
90
FibroGen Inc rhctgf
(A) HDMECs were transduced <t>with</t> <t>adenoviruses</t> Go, CTGF,CT and NT of CTGF at MOI10 for 24hrs; 25 μg of total proteins were separated via SDS-PAGE for MMP1, MMP9 and transferred to a nitrocellulose membrane. The blots were probed overnight with primary Abs at 4°C. As a control for equal protein loading, membranes were stripped and reprobed for β-actin. (B) Graphical representation of MMP1 and MMP9 protein expression determined by Western blot analysis presented in (A). The values represent the mean ± SEM of three independent experiment done in duplicate. *Significant values at p<0.05 (C) HDMECs were transduced with non silencing (NS) or AdCTGF siRNA with addition of S1P. Blocking endogenous CTGF inhibited S1P stimulated cells migration. (D) HDMECs were transduced with increasing doses of AdN-terminal (AdNT) and AdC-terminal (AdCT) domain of CTGF and expression level was determined by Western Blot analysis. (E) Graphical presentation of AdNT and AdCT expression level presented in (D). (F) HDMECs were cultured to 80% confluence in full medium followed by transduction with adenoviruses (AdGo, AdCTGF, AdNT and AdCT) or treated with <t>rhCTGF.</t> After 8 hrs cells were mechanically “wounded” by scraping with a Fisher-brand ready-tip. For the next 24-72 hrs cells were kept in EBM2 medium containing 0.5%serum. Representative images are presented. (G) The bar graph represents migration rate expressed as percentage of cells that crossed into the “scratch” area in comparison to identical “nonscratch” area. Cell counting was performed 48 hr after injury. The experiments were repeated three times in duplicate. (H) Graphical presentation of the transwell co-culture migration assay. Briefly, dermal fibroblasts were transduced with indicated adenoviruses. Next day, the medium was changed and HDMECs were plated on the cell culture inserts. 24 hr later the inserts were removed and cells were counted as described in the Methods section. (I) Bar graph presents endothelial cell proliferation increased after cells transduction with AdCTGF and AdCT. The values represent the mean ± SEM of three independent experiment done in duplicate.
Rhctgf, supplied by FibroGen Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rhctgf/rhctgf/pmc03176038-216-18-19
Average 90 stars, based on 1 article reviews
rhctgf - by Bioz Stars, 2026-10
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rhctgf (ctgf expressed cho cells affinity purified anti-ctgf antibody column  (FibroGen Inc)
90
FibroGen Inc rhctgf (ctgf expressed cho cells affinity purified anti-ctgf antibody column
(A) HDMECs were transduced <t>with</t> <t>adenoviruses</t> Go, CTGF,CT and NT of CTGF at MOI10 for 24hrs; 25 μg of total proteins were separated via SDS-PAGE for MMP1, MMP9 and transferred to a nitrocellulose membrane. The blots were probed overnight with primary Abs at 4°C. As a control for equal protein loading, membranes were stripped and reprobed for β-actin. (B) Graphical representation of MMP1 and MMP9 protein expression determined by Western blot analysis presented in (A). The values represent the mean ± SEM of three independent experiment done in duplicate. *Significant values at p<0.05 (C) HDMECs were transduced with non silencing (NS) or AdCTGF siRNA with addition of S1P. Blocking endogenous CTGF inhibited S1P stimulated cells migration. (D) HDMECs were transduced with increasing doses of AdN-terminal (AdNT) and AdC-terminal (AdCT) domain of CTGF and expression level was determined by Western Blot analysis. (E) Graphical presentation of AdNT and AdCT expression level presented in (D). (F) HDMECs were cultured to 80% confluence in full medium followed by transduction with adenoviruses (AdGo, AdCTGF, AdNT and AdCT) or treated with <t>rhCTGF.</t> After 8 hrs cells were mechanically “wounded” by scraping with a Fisher-brand ready-tip. For the next 24-72 hrs cells were kept in EBM2 medium containing 0.5%serum. Representative images are presented. (G) The bar graph represents migration rate expressed as percentage of cells that crossed into the “scratch” area in comparison to identical “nonscratch” area. Cell counting was performed 48 hr after injury. The experiments were repeated three times in duplicate. (H) Graphical presentation of the transwell co-culture migration assay. Briefly, dermal fibroblasts were transduced with indicated adenoviruses. Next day, the medium was changed and HDMECs were plated on the cell culture inserts. 24 hr later the inserts were removed and cells were counted as described in the Methods section. (I) Bar graph presents endothelial cell proliferation increased after cells transduction with AdCTGF and AdCT. The values represent the mean ± SEM of three independent experiment done in duplicate.
Rhctgf (Ctgf Expressed Cho Cells Affinity Purified Anti Ctgf Antibody Column, supplied by FibroGen Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rhctgf/rhctgf++ctgf+expressed+cho+cells+affinity+purified+anti+ctgf+antibody+column/pmc05860844-190-6-20
Average 90 stars, based on 1 article reviews
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rhctgf (ctgf100, containing only the carboxy-terminal domain iv of ctgf)  (EMP Genetech)
90
EMP Genetech rhctgf (ctgf100, containing only the carboxy-terminal domain iv of ctgf)
(A) HDMECs were transduced <t>with</t> <t>adenoviruses</t> Go, CTGF,CT and NT of CTGF at MOI10 for 24hrs; 25 μg of total proteins were separated via SDS-PAGE for MMP1, MMP9 and transferred to a nitrocellulose membrane. The blots were probed overnight with primary Abs at 4°C. As a control for equal protein loading, membranes were stripped and reprobed for β-actin. (B) Graphical representation of MMP1 and MMP9 protein expression determined by Western blot analysis presented in (A). The values represent the mean ± SEM of three independent experiment done in duplicate. *Significant values at p<0.05 (C) HDMECs were transduced with non silencing (NS) or AdCTGF siRNA with addition of S1P. Blocking endogenous CTGF inhibited S1P stimulated cells migration. (D) HDMECs were transduced with increasing doses of AdN-terminal (AdNT) and AdC-terminal (AdCT) domain of CTGF and expression level was determined by Western Blot analysis. (E) Graphical presentation of AdNT and AdCT expression level presented in (D). (F) HDMECs were cultured to 80% confluence in full medium followed by transduction with adenoviruses (AdGo, AdCTGF, AdNT and AdCT) or treated with <t>rhCTGF.</t> After 8 hrs cells were mechanically “wounded” by scraping with a Fisher-brand ready-tip. For the next 24-72 hrs cells were kept in EBM2 medium containing 0.5%serum. Representative images are presented. (G) The bar graph represents migration rate expressed as percentage of cells that crossed into the “scratch” area in comparison to identical “nonscratch” area. Cell counting was performed 48 hr after injury. The experiments were repeated three times in duplicate. (H) Graphical presentation of the transwell co-culture migration assay. Briefly, dermal fibroblasts were transduced with indicated adenoviruses. Next day, the medium was changed and HDMECs were plated on the cell culture inserts. 24 hr later the inserts were removed and cells were counted as described in the Methods section. (I) Bar graph presents endothelial cell proliferation increased after cells transduction with AdCTGF and AdCT. The values represent the mean ± SEM of three independent experiment done in duplicate.
Rhctgf (Ctgf100, Containing Only The Carboxy Terminal Domain Iv Of Ctgf), supplied by EMP Genetech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rhctgf/rhctgf++ctgf100++containing+only+the+carboxy+terminal+domain+iv+of+ctgf+/pm19309720-28-11-24
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recombinant human connective tissue growth factor (rhctgf) (n)  (CytoLab Ltd)
90
CytoLab Ltd recombinant human connective tissue growth factor (rhctgf) (n)
(A) HDMECs were transduced <t>with</t> <t>adenoviruses</t> Go, CTGF,CT and NT of CTGF at MOI10 for 24hrs; 25 μg of total proteins were separated via SDS-PAGE for MMP1, MMP9 and transferred to a nitrocellulose membrane. The blots were probed overnight with primary Abs at 4°C. As a control for equal protein loading, membranes were stripped and reprobed for β-actin. (B) Graphical representation of MMP1 and MMP9 protein expression determined by Western blot analysis presented in (A). The values represent the mean ± SEM of three independent experiment done in duplicate. *Significant values at p<0.05 (C) HDMECs were transduced with non silencing (NS) or AdCTGF siRNA with addition of S1P. Blocking endogenous CTGF inhibited S1P stimulated cells migration. (D) HDMECs were transduced with increasing doses of AdN-terminal (AdNT) and AdC-terminal (AdCT) domain of CTGF and expression level was determined by Western Blot analysis. (E) Graphical presentation of AdNT and AdCT expression level presented in (D). (F) HDMECs were cultured to 80% confluence in full medium followed by transduction with adenoviruses (AdGo, AdCTGF, AdNT and AdCT) or treated with <t>rhCTGF.</t> After 8 hrs cells were mechanically “wounded” by scraping with a Fisher-brand ready-tip. For the next 24-72 hrs cells were kept in EBM2 medium containing 0.5%serum. Representative images are presented. (G) The bar graph represents migration rate expressed as percentage of cells that crossed into the “scratch” area in comparison to identical “nonscratch” area. Cell counting was performed 48 hr after injury. The experiments were repeated three times in duplicate. (H) Graphical presentation of the transwell co-culture migration assay. Briefly, dermal fibroblasts were transduced with indicated adenoviruses. Next day, the medium was changed and HDMECs were plated on the cell culture inserts. 24 hr later the inserts were removed and cells were counted as described in the Methods section. (I) Bar graph presents endothelial cell proliferation increased after cells transduction with AdCTGF and AdCT. The values represent the mean ± SEM of three independent experiment done in duplicate.
Recombinant Human Connective Tissue Growth Factor (Rhctgf) (N), supplied by CytoLab Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rhctgf/recombinant+human+connective+tissue+growth+factor++rhctgf+++n+/pm16828074-40-3-27
Average 90 stars, based on 1 article reviews
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Image Search Results


(A) HDMECs were transduced with adenoviruses Go, CTGF,CT and NT of CTGF at MOI10 for 24hrs; 25 μg of total proteins were separated via SDS-PAGE for MMP1, MMP9 and transferred to a nitrocellulose membrane. The blots were probed overnight with primary Abs at 4°C. As a control for equal protein loading, membranes were stripped and reprobed for β-actin. (B) Graphical representation of MMP1 and MMP9 protein expression determined by Western blot analysis presented in (A). The values represent the mean ± SEM of three independent experiment done in duplicate. *Significant values at p<0.05 (C) HDMECs were transduced with non silencing (NS) or AdCTGF siRNA with addition of S1P. Blocking endogenous CTGF inhibited S1P stimulated cells migration. (D) HDMECs were transduced with increasing doses of AdN-terminal (AdNT) and AdC-terminal (AdCT) domain of CTGF and expression level was determined by Western Blot analysis. (E) Graphical presentation of AdNT and AdCT expression level presented in (D). (F) HDMECs were cultured to 80% confluence in full medium followed by transduction with adenoviruses (AdGo, AdCTGF, AdNT and AdCT) or treated with rhCTGF. After 8 hrs cells were mechanically “wounded” by scraping with a Fisher-brand ready-tip. For the next 24-72 hrs cells were kept in EBM2 medium containing 0.5%serum. Representative images are presented. (G) The bar graph represents migration rate expressed as percentage of cells that crossed into the “scratch” area in comparison to identical “nonscratch” area. Cell counting was performed 48 hr after injury. The experiments were repeated three times in duplicate. (H) Graphical presentation of the transwell co-culture migration assay. Briefly, dermal fibroblasts were transduced with indicated adenoviruses. Next day, the medium was changed and HDMECs were plated on the cell culture inserts. 24 hr later the inserts were removed and cells were counted as described in the Methods section. (I) Bar graph presents endothelial cell proliferation increased after cells transduction with AdCTGF and AdCT. The values represent the mean ± SEM of three independent experiment done in duplicate.

Journal:

Article Title: Connective Tissue Growth Factor (CTGF/CCN2) mediates angiogenic effect of S1P in human dermal microvascular endothelial cells

doi: 10.1111/j.1549-8719.2010.00058.x

Figure Lengend Snippet: (A) HDMECs were transduced with adenoviruses Go, CTGF,CT and NT of CTGF at MOI10 for 24hrs; 25 μg of total proteins were separated via SDS-PAGE for MMP1, MMP9 and transferred to a nitrocellulose membrane. The blots were probed overnight with primary Abs at 4°C. As a control for equal protein loading, membranes were stripped and reprobed for β-actin. (B) Graphical representation of MMP1 and MMP9 protein expression determined by Western blot analysis presented in (A). The values represent the mean ± SEM of three independent experiment done in duplicate. *Significant values at p<0.05 (C) HDMECs were transduced with non silencing (NS) or AdCTGF siRNA with addition of S1P. Blocking endogenous CTGF inhibited S1P stimulated cells migration. (D) HDMECs were transduced with increasing doses of AdN-terminal (AdNT) and AdC-terminal (AdCT) domain of CTGF and expression level was determined by Western Blot analysis. (E) Graphical presentation of AdNT and AdCT expression level presented in (D). (F) HDMECs were cultured to 80% confluence in full medium followed by transduction with adenoviruses (AdGo, AdCTGF, AdNT and AdCT) or treated with rhCTGF. After 8 hrs cells were mechanically “wounded” by scraping with a Fisher-brand ready-tip. For the next 24-72 hrs cells were kept in EBM2 medium containing 0.5%serum. Representative images are presented. (G) The bar graph represents migration rate expressed as percentage of cells that crossed into the “scratch” area in comparison to identical “nonscratch” area. Cell counting was performed 48 hr after injury. The experiments were repeated three times in duplicate. (H) Graphical presentation of the transwell co-culture migration assay. Briefly, dermal fibroblasts were transduced with indicated adenoviruses. Next day, the medium was changed and HDMECs were plated on the cell culture inserts. 24 hr later the inserts were removed and cells were counted as described in the Methods section. (I) Bar graph presents endothelial cell proliferation increased after cells transduction with AdCTGF and AdCT. The values represent the mean ± SEM of three independent experiment done in duplicate.

Article Snippet: After transduction with adenoviruses (Go, full length CTGF, N-terminus CTGF, C-terminus CTGF) or treated with rhCTGF (EMP Genetech, Germany) cells were cultured to confluence in EBM-2 medium containing 5% serum.

Techniques: Transduction, SDS Page, Membrane, Control, Expressing, Western Blot, Blocking Assay, Migration, Cell Culture, Comparison, Cell Counting, Co-Culture Assay