rhcd46 Search Results


rhcd46  (R&D Systems)
96
R&D Systems rhcd46
Rhcd46, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rhcd46/product/R&D Systems
Average 96 stars, based on 1 article reviews
rhcd46 - by Bioz Stars, 2026-02
96/100 stars
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recombinant human cd46 (rhcd46) protein  (Kemper GmbH)
90
Kemper GmbH recombinant human cd46 (rhcd46) protein
(a) Newcastle preparation, after dialysis (Lane 1, 20 µl and lane 2, 10 µl), or St. Louis prep (lane 5), were loaded in non-reducing SDS–PAGE buffer (irrelevant lanes are not shown). (b) 400 ng London (1), St. Louis (2) and Newcastle (3) <t>rhCD46</t> prep., or BSA (4) were run on 15% SDS–PAGE and blotted. rhCD46 was detected using GB24 and shee panti-mouse – HRPO (both 1:1000) and a 1 min exposure is shown for each blot. (c) Again 400 ng of each preparation and BSA (1–4 as above) were loaded onto two identical15% gels and blotted. The Nitrocellulose was divided in two and then probed with GB24 or P1 sera (1/300) or P1 IgG (1/50) or BDC sera (1/300) as indicated. The four ‘strips’ were aligned and exposed for 5 min. (d) Coomassie stained gel of GB24 purified Newcastle produced rhCD46. “Page ruler markers” are indicated throughout.
Recombinant Human Cd46 (Rhcd46) Protein, supplied by Kemper GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/recombinant human cd46 (rhcd46) protein/product/Kemper GmbH
Average 90 stars, based on 1 article reviews
recombinant human cd46 (rhcd46) protein - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
rhcd46 his-tag protein (10256-cd)  (R&D Systems Hematology)
90
R&D Systems Hematology rhcd46 his-tag protein (10256-cd)
(a) Newcastle preparation, after dialysis (Lane 1, 20 µl and lane 2, 10 µl), or St. Louis prep (lane 5), were loaded in non-reducing SDS–PAGE buffer (irrelevant lanes are not shown). (b) 400 ng London (1), St. Louis (2) and Newcastle (3) <t>rhCD46</t> prep., or BSA (4) were run on 15% SDS–PAGE and blotted. rhCD46 was detected using GB24 and shee panti-mouse – HRPO (both 1:1000) and a 1 min exposure is shown for each blot. (c) Again 400 ng of each preparation and BSA (1–4 as above) were loaded onto two identical15% gels and blotted. The Nitrocellulose was divided in two and then probed with GB24 or P1 sera (1/300) or P1 IgG (1/50) or BDC sera (1/300) as indicated. The four ‘strips’ were aligned and exposed for 5 min. (d) Coomassie stained gel of GB24 purified Newcastle produced rhCD46. “Page ruler markers” are indicated throughout.
Rhcd46 His Tag Protein (10256 Cd), supplied by R&D Systems Hematology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rhcd46 his-tag protein (10256-cd)/product/R&D Systems Hematology
Average 90 stars, based on 1 article reviews
rhcd46 his-tag protein (10256-cd) - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier

Image Search Results


(a) Newcastle preparation, after dialysis (Lane 1, 20 µl and lane 2, 10 µl), or St. Louis prep (lane 5), were loaded in non-reducing SDS–PAGE buffer (irrelevant lanes are not shown). (b) 400 ng London (1), St. Louis (2) and Newcastle (3) rhCD46 prep., or BSA (4) were run on 15% SDS–PAGE and blotted. rhCD46 was detected using GB24 and shee panti-mouse – HRPO (both 1:1000) and a 1 min exposure is shown for each blot. (c) Again 400 ng of each preparation and BSA (1–4 as above) were loaded onto two identical15% gels and blotted. The Nitrocellulose was divided in two and then probed with GB24 or P1 sera (1/300) or P1 IgG (1/50) or BDC sera (1/300) as indicated. The four ‘strips’ were aligned and exposed for 5 min. (d) Coomassie stained gel of GB24 purified Newcastle produced rhCD46. “Page ruler markers” are indicated throughout.

Journal: Molecular immunology

Article Title: Autoantibodies to CD59, CD55, CD46 or CD35 are not associated with atypical haemolytic uraemic syndrome (aHUS)

doi: 10.1016/j.molimm.2014.07.017

Figure Lengend Snippet: (a) Newcastle preparation, after dialysis (Lane 1, 20 µl and lane 2, 10 µl), or St. Louis prep (lane 5), were loaded in non-reducing SDS–PAGE buffer (irrelevant lanes are not shown). (b) 400 ng London (1), St. Louis (2) and Newcastle (3) rhCD46 prep., or BSA (4) were run on 15% SDS–PAGE and blotted. rhCD46 was detected using GB24 and shee panti-mouse – HRPO (both 1:1000) and a 1 min exposure is shown for each blot. (c) Again 400 ng of each preparation and BSA (1–4 as above) were loaded onto two identical15% gels and blotted. The Nitrocellulose was divided in two and then probed with GB24 or P1 sera (1/300) or P1 IgG (1/50) or BDC sera (1/300) as indicated. The four ‘strips’ were aligned and exposed for 5 min. (d) Coomassie stained gel of GB24 purified Newcastle produced rhCD46. “Page ruler markers” are indicated throughout.

Article Snippet: Testing and purification of E. coli produced recombinantCD46 (membrane cofactor protein, MCP) We originally received a batch of recombinant human CD46 (rhCD46) protein, generated in E. coli from Dr Claudia Kemper’s group (London, UK) and used this to screen our BDC and aHUS cohorts for the presence of autoantibodies to CD46.

Techniques: SDS Page, Staining, Purification, Produced

(a) A standard curve based on the mAb GB24 (1 µg/ml) binding to rhCD46 using ELISA is shown, allowing RU values to be assigned to test samples in the ELISA. (b) Indicates the relative unit (RU) values of 100 healthy blood donor controls (BDC) and 89 aHUS patient samples. Mean RU values are indicated by the solid line in each cohort and a dashed line representing the 97.5 percentile of the BDC group is also indicated. (c) The highest reacting BDC and aHUS samples, as indicated, were applied to nitrocellulose strips from a Western blotted rhCD46 prep gel. Each strip (delineated by vertical bars) was aligned prior to exposure for 20 min. Irrelevant intervening strips have been removed in this picture. GB24 was used as a positive control for protein loading and position. Molecular weight markers are shown and results are representative of two experiments.

Journal: Molecular immunology

Article Title: Autoantibodies to CD59, CD55, CD46 or CD35 are not associated with atypical haemolytic uraemic syndrome (aHUS)

doi: 10.1016/j.molimm.2014.07.017

Figure Lengend Snippet: (a) A standard curve based on the mAb GB24 (1 µg/ml) binding to rhCD46 using ELISA is shown, allowing RU values to be assigned to test samples in the ELISA. (b) Indicates the relative unit (RU) values of 100 healthy blood donor controls (BDC) and 89 aHUS patient samples. Mean RU values are indicated by the solid line in each cohort and a dashed line representing the 97.5 percentile of the BDC group is also indicated. (c) The highest reacting BDC and aHUS samples, as indicated, were applied to nitrocellulose strips from a Western blotted rhCD46 prep gel. Each strip (delineated by vertical bars) was aligned prior to exposure for 20 min. Irrelevant intervening strips have been removed in this picture. GB24 was used as a positive control for protein loading and position. Molecular weight markers are shown and results are representative of two experiments.

Article Snippet: Testing and purification of E. coli produced recombinantCD46 (membrane cofactor protein, MCP) We originally received a batch of recombinant human CD46 (rhCD46) protein, generated in E. coli from Dr Claudia Kemper’s group (London, UK) and used this to screen our BDC and aHUS cohorts for the presence of autoantibodies to CD46.

Techniques: Binding Assay, Enzyme-linked Immunosorbent Assay, Western Blot, Stripping Membranes, Positive Control, Molecular Weight