rhbdl4 Search Results


pcmv6 rhbdl4mycflag  (OriGene)
90
OriGene pcmv6 rhbdl4mycflag
Identification of PTP1B (also called PTPN1) in the BioID and as interactor of RHBDL4. A , peptide overage of PTP1B in the BioID of RHBDL4. Peptides identified by MS are shown in blue. B , the substrate-trapping mutant D181A and the super-trapping mutant D181A/Y46F of mCherry-PTP1B were transiently expressed with or without <t>RHBDL4mycFLAG.</t> The cells were lysed in immunoprecipitation ( IP )–compatible conditions and captured with anti-FLAG immunomagnetic beads. Co-immunoprecipitates and whole cell lysates ( Input ) were probed with anti-mCherry, or RHBDL4 antibody. C , HEK293 cells expressing TMEM115-mycFLAG or RHBDL4mycFLAG were lysed and bound with anti-FLAG beads ± 4 m m Na 3 VO 4 . The resulting co-immunoprecipitates were probed with anti-FLAG-HRP or anti-mCherry antibody.
Pcmv6 Rhbdl4mycflag, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rhbdl4/pmc06663880-210-14-19?v=OriGene
Average 90 stars, based on 1 article reviews
pcmv6 rhbdl4mycflag - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier
rhbdl4 protein  (Gallus BioPharmaceuticals)
90
Gallus BioPharmaceuticals rhbdl4 protein
Identification of PTP1B (also called PTPN1) in the BioID and as interactor of RHBDL4. A , peptide overage of PTP1B in the BioID of RHBDL4. Peptides identified by MS are shown in blue. B , the substrate-trapping mutant D181A and the super-trapping mutant D181A/Y46F of mCherry-PTP1B were transiently expressed with or without <t>RHBDL4mycFLAG.</t> The cells were lysed in immunoprecipitation ( IP )–compatible conditions and captured with anti-FLAG immunomagnetic beads. Co-immunoprecipitates and whole cell lysates ( Input ) were probed with anti-mCherry, or RHBDL4 antibody. C , HEK293 cells expressing TMEM115-mycFLAG or RHBDL4mycFLAG were lysed and bound with anti-FLAG beads ± 4 m m Na 3 VO 4 . The resulting co-immunoprecipitates were probed with anti-FLAG-HRP or anti-mCherry antibody.
Rhbdl4 Protein, supplied by Gallus BioPharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rhbdl4/pmc10920636__41467_2024_45615_MOESM1_ESM-39-11-19?v=Gallus+BioPharmaceuticals
Average 90 stars, based on 1 article reviews
rhbdl4 protein - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier
mouse models for rhbdl4  (International Mouse Phenotyping Consortium)
86
International Mouse Phenotyping Consortium mouse models for rhbdl4
Identification of PTP1B (also called PTPN1) in the BioID and as interactor of RHBDL4. A , peptide overage of PTP1B in the BioID of RHBDL4. Peptides identified by MS are shown in blue. B , the substrate-trapping mutant D181A and the super-trapping mutant D181A/Y46F of mCherry-PTP1B were transiently expressed with or without <t>RHBDL4mycFLAG.</t> The cells were lysed in immunoprecipitation ( IP )–compatible conditions and captured with anti-FLAG immunomagnetic beads. Co-immunoprecipitates and whole cell lysates ( Input ) were probed with anti-mCherry, or RHBDL4 antibody. C , HEK293 cells expressing TMEM115-mycFLAG or RHBDL4mycFLAG were lysed and bound with anti-FLAG beads ± 4 m m Na 3 VO 4 . The resulting co-immunoprecipitates were probed with anti-FLAG-HRP or anti-mCherry antibody.
Mouse Models For Rhbdl4, supplied by International Mouse Phenotyping Consortium, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rhbdl4/pmc12476590-73-8-31?v=International+Mouse+Phenotyping+Consortium
Average 86 stars, based on 1 article reviews
mouse models for rhbdl4 - by Bioz Stars, 2026-08
86/100 stars
  Buy from Supplier

Image Search Results


Identification of PTP1B (also called PTPN1) in the BioID and as interactor of RHBDL4. A , peptide overage of PTP1B in the BioID of RHBDL4. Peptides identified by MS are shown in blue. B , the substrate-trapping mutant D181A and the super-trapping mutant D181A/Y46F of mCherry-PTP1B were transiently expressed with or without RHBDL4mycFLAG. The cells were lysed in immunoprecipitation ( IP )–compatible conditions and captured with anti-FLAG immunomagnetic beads. Co-immunoprecipitates and whole cell lysates ( Input ) were probed with anti-mCherry, or RHBDL4 antibody. C , HEK293 cells expressing TMEM115-mycFLAG or RHBDL4mycFLAG were lysed and bound with anti-FLAG beads ± 4 m m Na 3 VO 4 . The resulting co-immunoprecipitates were probed with anti-FLAG-HRP or anti-mCherry antibody.

Journal: The Journal of Biological Chemistry

Article Title: Spatial proteomics reveal that the protein phosphatase PTP1B interacts with and may modify tyrosine phosphorylation of the rhomboid protease RHBDL4

doi: 10.1074/jbc.RA118.007074

Figure Lengend Snippet: Identification of PTP1B (also called PTPN1) in the BioID and as interactor of RHBDL4. A , peptide overage of PTP1B in the BioID of RHBDL4. Peptides identified by MS are shown in blue. B , the substrate-trapping mutant D181A and the super-trapping mutant D181A/Y46F of mCherry-PTP1B were transiently expressed with or without RHBDL4mycFLAG. The cells were lysed in immunoprecipitation ( IP )–compatible conditions and captured with anti-FLAG immunomagnetic beads. Co-immunoprecipitates and whole cell lysates ( Input ) were probed with anti-mCherry, or RHBDL4 antibody. C , HEK293 cells expressing TMEM115-mycFLAG or RHBDL4mycFLAG were lysed and bound with anti-FLAG beads ± 4 m m Na 3 VO 4 . The resulting co-immunoprecipitates were probed with anti-FLAG-HRP or anti-mCherry antibody.

Article Snippet: All point mutations were introduced by site-directed mutagenesis using CloneAmp HiFI PCR premix (Clontech). pCMV6 RHBDL4mycFLAG was purchased from Origene (RC210708).

Techniques: Mutagenesis, Immunoprecipitation, Expressing

Co-immunoprecipitation ( IP ) of Lys 63 -linked ubiquitin and p97/VCP with mutants of RHBDL4. HEK293 cells expressing myc-FLAG–tagged mutants of RHBDL4 for site 1 (Y206F), site 7 (Y261F), site 8 (Y264F), and all-sites mutant were lysed and incubated with anti-FLAG beads. Co-immunoprecipitates and whole cell lysates ( Input ) were probed with anti-p97/VCP ( panel a ), anti-ubiquitin ( panel b ), anti-Lys 63 linkage–specific antibodies ( panel c ), and anti-FLAG-HRP for RHBDL4mycFLAG ( panel d ). B , relative quantification of the immunoreactivity of co-immunoprecipitates ( panel a ) versus anti-p97/VCP (*, p = 0.014, n = 3) ( panel b ) or anti-Lys 63 -linked ubiquitin (*, p = 0.024, n = 3) of WT RHBDL4mycFLAG and AllY→F, normalized against the input. The error bars represent standard deviation from the means.

Journal: The Journal of Biological Chemistry

Article Title: Spatial proteomics reveal that the protein phosphatase PTP1B interacts with and may modify tyrosine phosphorylation of the rhomboid protease RHBDL4

doi: 10.1074/jbc.RA118.007074

Figure Lengend Snippet: Co-immunoprecipitation ( IP ) of Lys 63 -linked ubiquitin and p97/VCP with mutants of RHBDL4. HEK293 cells expressing myc-FLAG–tagged mutants of RHBDL4 for site 1 (Y206F), site 7 (Y261F), site 8 (Y264F), and all-sites mutant were lysed and incubated with anti-FLAG beads. Co-immunoprecipitates and whole cell lysates ( Input ) were probed with anti-p97/VCP ( panel a ), anti-ubiquitin ( panel b ), anti-Lys 63 linkage–specific antibodies ( panel c ), and anti-FLAG-HRP for RHBDL4mycFLAG ( panel d ). B , relative quantification of the immunoreactivity of co-immunoprecipitates ( panel a ) versus anti-p97/VCP (*, p = 0.014, n = 3) ( panel b ) or anti-Lys 63 -linked ubiquitin (*, p = 0.024, n = 3) of WT RHBDL4mycFLAG and AllY→F, normalized against the input. The error bars represent standard deviation from the means.

Article Snippet: All point mutations were introduced by site-directed mutagenesis using CloneAmp HiFI PCR premix (Clontech). pCMV6 RHBDL4mycFLAG was purchased from Origene (RC210708).

Techniques: Immunoprecipitation, Ubiquitin Proteomics, Expressing, Mutagenesis, Incubation, Quantitative Proteomics, Standard Deviation