Journal: iScience

Article Title: Distinct effects of sacituzumab govitecan and berzosertib on DNA damage response in ovarian cancer

doi: 10.1016/j.isci.2024.111283

Figure Lengend Snippet:

Article Snippet: Rabbit anti- MAD2L2/REV7 , ProteinTech , Cat #12683-1-AP; RRID: AB_2139530.

Techniques: Recombinant, Staining, Western Blot, Stripping, Control, SYBR Green Assay, cDNA Synthesis, Gene Expression, Software, Microscopy, Imaging

Journal: Advanced Science

Article Title: A Novel FGFR3‐Targeting Antibody‐Drug Conjugate Induces Tumor Cell Apoptosis through the cGAS–STING Pathway in Bladder Cancer

doi: 10.1002/advs.202509933

Figure Lengend Snippet: A2 induces apoptosis in BC cells by targeting MAD2L1. A) Reaction scheme for biotinylation at the 20th hydroxyl group of A2. B) Overview of differentially expressed proteins identified by MS. C) Top 10 proteins ranked by the A2‐biotin/biotin abundance ratio. D) Western blot analysis of MAD2L1 expression in pull‐down products. E) Western blot detection of soluble MAD2L1 in T24 cells treated with A2 at different temperatures ( n = 3). F) SPR analysis of A2‐MAD2L1 binding kinetics and KD value. G) Representative immunofluorescence images showing co‐localization of A2‐biotin and MAD2L1 in T24 cells after 3 h of A2‐biotin treatment. Red: Anti‐MAD2L1, green: A2‐biotin, blue: DAPI, scale bar = 20 µm. H) Western blot analysis of MAD2L1 expression in T24 and UMUC‐3 cells treated with different concentrations of A2 for 24 h. I) Western blot validation of MAD2L1 OE in T24 and UMUC‐3 cells. J) Western blot validation of MAD2L1 KD in T24 and UMUC‐3 cells. K) Colony formation capacity of MAD2L1‐OE and control cells treated with different concentrations of A2 for 48 h. L) Apoptotic effects in MAD2L1‐OE and control cells treated with different concentrations of A2 for 48 h. ( n = 3). M) Apoptotic effects in MAD2L1‐KD and control cells treated with different concentrations of A2 for 48 h ( n = 3). Data are presented as mean ± SD. **** P < 0.0001. Statistical significance was determined by two‐way ANOVA followed by Tukey's multiple comparison test (L,M).

Article Snippet: SPR experiments were performed using a CM5 chip with amine coupling to immobilize MAD2L1 protein (HY‐ P71540 , MCE, USA).

Techniques: Western Blot, Expressing, Binding Assay, Immunofluorescence, Biomarker Discovery, Control, Comparison

Journal: Advanced Science

Article Title: A Novel FGFR3‐Targeting Antibody‐Drug Conjugate Induces Tumor Cell Apoptosis through the cGAS–STING Pathway in Bladder Cancer

doi: 10.1002/advs.202509933

Figure Lengend Snippet: A2 specifically binds to the Lys73 site of MAD2L1. A) Molecular docking simulation of the interaction between A2 and MAD2L1. B) Western blot validation of MAD2L1 expression levels in control, MAD2L1‐KD, and mutant T24 cells. C) Western blot analysis of MAD2L1 expression in pull‐down products from each group. D–F CETSA evaluating the binding of A2 to MAD2L1 in MAD2L1‐KD T24 cells reconstituted with MAD2L1 WT (D), MAD2L1V55A+I62A+V69A (E), and MAD2L1K73A (F) ( n = 3). Data are presented as mean ± SD.

Article Snippet: SPR experiments were performed using a CM5 chip with amine coupling to immobilize MAD2L1 protein (HY‐ P71540 , MCE, USA).

Techniques: Western Blot, Biomarker Discovery, Expressing, Control, Mutagenesis, Binding Assay

Journal: Advanced Science

Article Title: A Novel FGFR3‐Targeting Antibody‐Drug Conjugate Induces Tumor Cell Apoptosis through the cGAS–STING Pathway in Bladder Cancer

doi: 10.1002/advs.202509933

Figure Lengend Snippet: A2 targets MAD2L1 to activate the cGAS‐STING pathway. A) Effect of A2 on aneuploidy formation in T24 and UMUC‐3 cells. B) Representative images showing cGAS subcellular distribution and micronuclei formation (indicated by arrows) in T24 and UMUC‐3 cells treated with 0.064 µ m A2 for 48 h. Red: cGAS, green: PicoGreen (DNA stain), Scale bar = 20 µm. C) Quantification of cytosolic DNA in T24 and UMUC‐3 cells treated with 0.32 µ m A2 for 48 h ( n = 3). D) Western blot analysis of cGAS‐STING pathway protein expression in T24 and UMUC‐3 cells treated with different concentrations of A2 for 48 h. E) Western blot analysis of cGAS‐STING pathway protein expression in MAD2L1‐OE and control cells treated with different concentrations of A2 for 48 h. F) Western blot analysis of cGAS‐STING pathway protein expression in MAD2L1‐KD and control cells treated with different concentrations of A2 for 48 h. G) Apoptotic effects in T24 and UMUC‐3 cells treated with 1.6 µ m A2 after STING inhibitor H151‐mediated blockade of the cGAS‐STING pathway. Data are presented as mean ± SD. **** P < 0.0001. Statistical significance was determined by a two‐tailed Student's t‐ test (C).

Article Snippet: SPR experiments were performed using a CM5 chip with amine coupling to immobilize MAD2L1 protein (HY‐ P71540 , MCE, USA).

Techniques: Staining, Western Blot, Expressing, Control, Two Tailed Test

Journal: Advanced Science

Article Title: A Novel FGFR3‐Targeting Antibody‐Drug Conjugate Induces Tumor Cell Apoptosis through the cGAS–STING Pathway in Bladder Cancer

doi: 10.1002/advs.202509933

Figure Lengend Snippet: Cytotoxicity of LZU‐WZLYCS01 against BC cells and PDOs, and validation of the bystander effect. A) Cell viability of WT and FGFR3‐KO T24 and UMUC‐3 cells treated with different concentrations of LZU‐WZLYCS01, A2, or FGFR3 antibody for 72 h. B) Apoptotic effects in WT and FGFR3‐KO T24 and UMUC‐3 cells treated with different concentrations of LZU‐WZLYCS01 for 48 h ( n = 3). C) Colony formation capacity of T24 and UMUC‐3 cells treated with LZU‐WZLYCS01 for 48 h. D) Schematic diagram of the co‐culture model for assessing the bystander effects. E) Effect of LZU‐WZLYCS01 on the viability of FGFR3‐KO T24 and UMUC‐3 cells in the co‐culture system ( n = 3). F) Representative bright‐field and AM/PI‐stained images showing LZU‐WZLYCS01‐induced cytotoxicity in PDOs. Green: viable cells, red: dead cells, scale bar = 50 µm. G) Representative H&E staining of PDOs and matched parental tumor tissues. Scale bar = 25 µm. H) Representative IHC staining of UPK2, FGFR3, and MAD2L1 in PDOs. Scale bar = 25 µm. Data are presented as mean ± SD. * P < 0.05, ** P < 0.01, **** P < 0.0001. Statistical significance was determined by two‐way ANOVA followed by Tukey's multiple comparison test (B) and a two‐tailed Student's t‐ test (E).

Article Snippet: SPR experiments were performed using a CM5 chip with amine coupling to immobilize MAD2L1 protein (HY‐ P71540 , MCE, USA).

Techniques: Biomarker Discovery, Co-Culture Assay, Staining, Immunohistochemistry, Comparison, Two Tailed Test

Journal: Advanced Science

Article Title: A Novel FGFR3‐Targeting Antibody‐Drug Conjugate Induces Tumor Cell Apoptosis through the cGAS–STING Pathway in Bladder Cancer

doi: 10.1002/advs.202509933

Figure Lengend Snippet: Mechanism of action and tumor‐targeting capability of LZU‐WZLYCS01. A) Flow cytometry analysis of LZU‐WZLYCS01 binding and internalization in T24 and UMUC‐3 cells at 37 and 4 °C. B) Representative images showing LZU‐WZLYCS01 binding, internalization, and lysosomal co‐localization in T24 and UMUC‐3 cells at 37 and 4 °C. Red: LZU‐WZLYCS01, green: anti‐LAMP2, blue: DAPI, scale bar = 10 µm. C) Western blot analysis of cGAS‐STING pathway protein expression in T24 and UMUC‐3 cells treated with different concentrations of LZU‐WZLYCS01 for 48 h. D) Western blot analysis of cGAS‐STING pathway protein expression in MAD2L1‐OE and control cells treated with different concentrations of LZU‐WZLYCS01 for 48 h. E) In vivo fluorescence imaging of UMUC‐3 xenograft models 24 h after intravenous injection of LZU‐WZLYCS01–Cy5 ( n = 3). F) Ex vivo fluorescence imaging of tumors and major organs (heart, liver, spleen, lungs, kidneys, and brain) collected 24 h post‐injection.

Article Snippet: SPR experiments were performed using a CM5 chip with amine coupling to immobilize MAD2L1 protein (HY‐ P71540 , MCE, USA).

Techniques: Flow Cytometry, Binding Assay, Western Blot, Expressing, Control, In Vivo, Fluorescence, Imaging, Injection, Ex Vivo

Journal: PLOS Biology

Article Title: Canonical and noncanonical roles of Hop1 are crucial for meiotic prophase in the fungus Sordaria macrospora

doi: 10.1371/journal.pbio.3002705

Figure Lengend Snippet: ( A ) Schematic domain organization of Sordaria Hop1. ( B ) AlphaFold2 modelled Sordaria Hop1 HORMA domain (left), atomic (PDB:4TZJ), and experimentally determined C . elegans HIM-3 (middle) share structural similarities as shown by their superimposition (HIM-3 gray, right). Alpha-helices and beta-strands are in red and green, respectively. ( C ) Localization of Hop1 in WT meiosis. Hop1-GFP (left) and Spo76/Pds5-TdTomato (Tred, middle) are perfectly colocalized in the 2 pre-karyogamy haploid nuclei (top) and from leptotene to the post-pachytene diffuse stage (bottom). Scale bars: 2 μm. SCD, S/TJQ cluster domain; WT, wild-type.

Article Snippet: In addition to Hop1, as for most eukaryotes, the S . macrospora genome contains also 2 other HORMA domain proteins: the subunit of DNA polymerase Zeta Rev7 (SMAC_04698) and the spindle-checkpoint protein Mad2 (SMAC_09019) ( ).

Techniques:

Journal: PLOS Biology

Article Title: Canonical and noncanonical roles of Hop1 are crucial for meiotic prophase in the fungus Sordaria macrospora

doi: 10.1371/journal.pbio.3002705

Figure Lengend Snippet: ( A ) Top: domain diagram of WT Hop1. Bottom: diagram of the deleted or mutated Hop1 sites in the 6 analyzed mutants (). ( B ) Protein localization and phenotypes of the hop1-HORMAΔ mutant. Top: In the hop1Δ background, the mutant protein tagged with mCherry (mC, left) is not visible along the axes, marked by Spo76-GFP (middle) and merge (right). Bottom: colocalization with Ecm11-GFP + Hei10-GFP (middle) indicates that only few SC segments (containing Hei10 foci, arrows) are formed in this mutant; right corresponding DAPI. ( C, D ) hop1-HORMAΔ SCs exhibit the same length ( C ) and Hei10 foci number ( D ) as hop1Δ . Mean and error bar (SD) are indicated for each set. Significance between WT, hop1Δ and hop1-HORMAΔ nuclei was established by Brown–Forsythe ANOVA test: ns = not significant, P -value > 0.05; n = 38 and 42 nuclei. ( E ) hop1-only-HORMA . Top: the HORMA domain alone is sufficient for axis localization of the protein but only as discontinuous segments (arrows) that colocalize with Spo76-GFP (arrows middle and merge right) in contrast to the continuous lines seen in WT with Hop1-mCherry (right). Bottom: colocalization of Hop1-only-HORMA-mC (left) with Ecm11-GFP and Hei10-GFP (middle) and merge (right) indicates that the visible mutant segments correspond to SCs. ( C ) The mutant SCs have the same length as hop1Δ SCs ( C ) but exhibit a slightly lower number of Hei10 foci ( D ): Brown–Forsythe ANOVA test, ns = not significant; n = 31 and 42 nuclei. Scale bars: 2 μm. The raw data underlying panels 6C and 6D are available in . SC, synaptonemal complex; WT, wild-type.

Article Snippet: In addition to Hop1, as for most eukaryotes, the S . macrospora genome contains also 2 other HORMA domain proteins: the subunit of DNA polymerase Zeta Rev7 (SMAC_04698) and the spindle-checkpoint protein Mad2 (SMAC_09019) ( ).

Techniques: Mutagenesis