Journal: Molecules

Article Title: In Vitro and In Vivo Activity of 14-O-[(4,6-Diamino-pyrimidine-2-yl) thioacetyl] Mutilin against Methicillin-Resistant Staphylococcus aureus

doi: 10.3390/molecules26113277

Figure Lengend Snippet: Figure 1. Chemical structures of pleuromutilin tiamulin, valnemulin, retapamulin, Lefamulin, and DPTM.

Article Snippet: Tiamulin fumarate (purity: 98.5%) was purchased from Labor Dr. Ehrenstorfer-Schäfers (Augsburg, Germany) and retapamulin (98.0%) was purchased from (BOC Sciences, New York, NY, USA).

Techniques:

Journal: Molecules

Article Title: In Vitro and In Vivo Activity of 14-O-[(4,6-Diamino-pyrimidine-2-yl) thioacetyl] Mutilin against Methicillin-Resistant Staphylococcus aureus

doi: 10.3390/molecules26113277

Figure Lengend Snippet: Figure 3. Macroscopic evaluation of wound at the 6th day in the infection model. Mice (n = 10 per group) were infected in the excision wound with MRSA ATCC43300 strain. (A) uninfected group, (B) control group, (C) 1% DPTM ointment treatment group, (D) 2% DPTM ointment treatment group, (E) 2% Retapamulin ointment treatment group, and (F) 3% DPTM ointment treatment group.

Article Snippet: Tiamulin fumarate (purity: 98.5%) was purchased from Labor Dr. Ehrenstorfer-Schäfers (Augsburg, Germany) and retapamulin (98.0%) was purchased from (BOC Sciences, New York, NY, USA).

Techniques: Infection, Control, Ointment

Journal: Molecules

Article Title: In Vitro and In Vivo Activity of 14-O-[(4,6-Diamino-pyrimidine-2-yl) thioacetyl] Mutilin against Methicillin-Resistant Staphylococcus aureus

doi: 10.3390/molecules26113277

Figure Lengend Snippet: Figure 4. Efficacy of DPTM (1%, 2%, and 3% ointment) and retapamulin (2% ointment) against an experimental surgical wound infection in mice (n = 5) caused by MRSA. Values are means and standard errors of the means. ** p < 0.01.

Article Snippet: Tiamulin fumarate (purity: 98.5%) was purchased from Labor Dr. Ehrenstorfer-Schäfers (Augsburg, Germany) and retapamulin (98.0%) was purchased from (BOC Sciences, New York, NY, USA).

Techniques: Ointment, Infection

Journal: Molecules

Article Title: In Vitro and In Vivo Activity of 14-O-[(4,6-Diamino-pyrimidine-2-yl) thioacetyl] Mutilin against Methicillin-Resistant Staphylococcus aureus

doi: 10.3390/molecules26113277

Figure Lengend Snippet: Figure 5. Effect of DPTM (1%, 2%, and 3% ointment) and retapamulin (2% ointment) on mice WBC. All values are mean ± SD. # p < 0.05 indicates that the blank group, 2% DPTM, 3% DPTM, and retapamulin ointment treatment group vs. control group; * p < 0.05 indicates that 1% DPTM ointment treatment group vs. 2% retapamulin group.

Article Snippet: Tiamulin fumarate (purity: 98.5%) was purchased from Labor Dr. Ehrenstorfer-Schäfers (Augsburg, Germany) and retapamulin (98.0%) was purchased from (BOC Sciences, New York, NY, USA).

Techniques: Ointment, Control

Journal: Molecules

Article Title: In Vitro and In Vivo Activity of 14-O-[(4,6-Diamino-pyrimidine-2-yl) thioacetyl] Mutilin against Methicillin-Resistant Staphylococcus aureus

doi: 10.3390/molecules26113277

Figure Lengend Snippet: Figure 6. Effect of DPTM on pathological changes in MRSA infected skin (HE: 200×, Masson: 100×). These microscopic photographs of the wound healing skins were obtained from mice in the blank control group (A1 and A2), positive control group (B1 and B2), 1% DPTM ointment treatment group (C1 and C2), 2% DPTM ointment treatment group (D1 and D2), 2% retapamulin ointment treatment group (E1 and E2), and 3% DPTM ointment treatment group (F1 and F2). Red arrows point at inflammatory cell infiltration and the blue arrows point at inflammatory cell accumulation.

Article Snippet: Tiamulin fumarate (purity: 98.5%) was purchased from Labor Dr. Ehrenstorfer-Schäfers (Augsburg, Germany) and retapamulin (98.0%) was purchased from (BOC Sciences, New York, NY, USA).

Techniques: Infection, Control, Positive Control, Ointment

Journal: Molecules

Article Title: In Vitro and In Vivo Activity of 14-O-[(4,6-Diamino-pyrimidine-2-yl) thioacetyl] Mutilin against Methicillin-Resistant Staphylococcus aureus

doi: 10.3390/molecules26113277

Figure Lengend Snippet: Figure 7. Effect of DPTM and retapamulin ointments on secretions of IL-6, TNF-α, and VEGF. IL-6 (A), TNF-α (B), and VEGF (C). All values are mean ± SD. # p < 0.05 indicates that the blank group, 2% DPTM, 3% DPTM, and 2% retapamulin ointment treatment group vs. the control group; * p < 0.05 indicates 1% DPTM ointment treatment group vs. the 2% retapamulin ointment group.

Article Snippet: Tiamulin fumarate (purity: 98.5%) was purchased from Labor Dr. Ehrenstorfer-Schäfers (Augsburg, Germany) and retapamulin (98.0%) was purchased from (BOC Sciences, New York, NY, USA).

Techniques: Ointment, Control

Journal: Cold Spring Harbor Perspectives in Medicine

Article Title: Pleuromutilins: Potent Drugs for Resistant Bugs—Mode of Action and Resistance

doi: 10.1101/cshperspect.a027110

Figure Lengend Snippet: Interaction network. (A) The tricyclic pleuromutilin core and sulfanylacetyl as well as acylcarbamate linker (side chains omitted for clarity), and (B) the C14 side chain extension with nucleotides of the peptidyl transferase center (PTC). Hydrogen bonds are shown as dotted lines (Schlunzen et al. 2004; Davidovich et al. 2007; Eyal et al. 2015).

Article Snippet: Despite significant efforts in the field of acylcarbamate pleuromutilins by the GlaxoSmithKline group ( Hunt 2000 ; Brooks et al. 2001 ; Andemichael et al. 2009 ), only sulfanylacetyl derivatives, mostly with one basic function at the side chain, have progressed beyond phase 1 clinical studies.

Techniques:

Journal: Molecules

Article Title: A Comprehensive Overview of the Antibiotics Approved in the Last Two Decades: Retrospects and Prospects

doi: 10.3390/molecules28041762

Figure Lengend Snippet: Summary of protein synthesis inhibitors that act on ribosomal subunits.

Article Snippet: Retapamulin ( 28 ) , Altabax , GlaxoSmithKline , 2007 , 224452-66-8 , Phagocytosis caused by Staphylococcus aureus or Streptococcus pyogenes , Ribosomal protein inhibitor.

Techniques: Infection