Journal: Archives of Rheumatology

Article Title: Associations of Mitochondrial Deoxyribonucleic Acid Polymorphisms With Behçet's Disease in the Korean Population

doi: 10.5606/ArchRheumatol.2019.7113

Figure Lengend Snippet: Polymerase chain reaction/Sanger sequencing primers used for m.5178 and mitochondrial deoxyribonucleic acid control region

Article Snippet: Eleven mitochondrial DNA nucleotides (m.709, m.3010, m.4883, m.6392, m.6962, m.10310, m.10609, m.12406, m.12882, m.13928, and m.14668) were genotyped using the MassARRAY system (Agena BioScience Inc., San Diego, CA, USA) with the Assay Design Suite tool and iPLEX® assay.

Techniques: Sequencing, Control, Polymerase Chain Reaction

Journal: Archives of Rheumatology

Article Title: Associations of Mitochondrial Deoxyribonucleic Acid Polymorphisms With Behçet's Disease in the Korean Population

doi: 10.5606/ArchRheumatol.2019.7113

Figure Lengend Snippet: Associations between symptoms of Behçet's disease and mitochondrial deoxyribonucleic acid alterations

Article Snippet: Eleven mitochondrial DNA nucleotides (m.709, m.3010, m.4883, m.6392, m.6962, m.10310, m.10609, m.12406, m.12882, m.13928, and m.14668) were genotyped using the MassARRAY system (Agena BioScience Inc., San Diego, CA, USA) with the Assay Design Suite tool and iPLEX® assay.

Techniques:

Journal:

Article Title: Human Urine Contains Small, 150 to 250 Nucleotide-Sized, Soluble DNA Derived from the Circulation and May Be Useful in the Detection of Colorectal Cancer

doi:

Figure Lengend Snippet: Wild-type and mutant K-ras sequences detected in human urine and disease tissue by restriction-enriched polymerase chain reactions (RE-PCR). A: The predicted pattern of digested products. B: The results of analysis of DNA derived from the urine of an individual with no diagnosis of cancer (Zu) and from tumor tissue (AAt), non-tumor tissue that was 1 cm apart from the tumor (AAadj), serum (AAs), and urine (AAu) from an individual with CRC. C: The sensitivity and specificity of the assay by analysis of the reconstruction standards (1.5, 15, and 150 copies of SW480 genome per 50 ng of HepG2 DNA), and 50 ng HepG2 DNA and 5 ng SW480 alone, as indicated. D: Detection of mutated K-ras DNA in CRC tissue. CRC disease tissue sections from five CRC patients (Ot, AAt, AMt, BQt, and DGt) were subjected to DNA isolation and the RE-PCR assay for mutated K-ras DNA. MW: DNA MW markers. This represents the data from three independent experiments.

Article Snippet: One ml of resin (Wizard DNA Isolation Kit, Promega, Madison, WI) was added into the urine lysate and incubated for 2 hours at room temperature with gentle mixing.

Techniques: Mutagenesis, Derivative Assay, Biomarker Discovery, DNA Extraction

Journal: International Journal of Obesity (2005)

Article Title: Tankyrase inhibition ameliorates lipid disorder via suppression of PGC-1α PARylation in db/db mice

doi: 10.1038/s41366-020-0573-z

Figure Lengend Snippet: a Immunoprecipitation of muscle lysates from normal mice with TNKS1/2 antibody and rabbit IgG as a control. Fractions of the immunoprecipitates were separated in individual lanes and immunoblotted with anti-TNKS1/2 and anti-PGC-1α IgGs. b–d Duolink in situ PLA showing association of TNKS1 with PGC-1α in muscle of control db/db mice. Representative image of TNKS1/2-PGC-1α association ( b ) and PLA performed with either anti-PGC-1α IgG ( c ) or anti-TNKS1/2 IgG ( d ) alone. Scale bar, 50 μm. e Pull-down assay of muscle lysate from normal mice with PAR-affinity resin (WWE) followed by immunoblotting with anti-PGC-1α or anti-TNKS1/2. Control: negative control resin harboring a mutation in WWE that abolishes PAR binding. f – j Duolink in situ PLA showing PARylation of PGC-1α in muscle of G007-LK-treated and control db/db mice. Representative image of PARylated PGC-1α in muscle in control ( f ) and G007-LK-treated ( g ) db/db mice. PLA performed with either anti-PGC-1α IgG ( h ) or anti-PAR IgG ( i ) alone. Quantification of PGC-1α PARylation level ( j ). Scale bar, 50 µm. k – o Duolink in situ PLA showing association of TNKS1 with PGC-1α in WAT of G007-LK-treated and control db/db mice. Representative image of TNKS1/2-PGC-1α association in control ( k ) and G007-LK-treated db/db mice ( i ). PLA performed with either anti-PGC-1α IgG ( m ) or anti-TNKS1/2 IgG ( n ) alone. Quantification of PGC-1α and TNKS1/2 association ( o ). Scale bar, 50 μm. p – t Duolink in situ PLA showing PARylation of PGC-1α in WAT of G007-LK-treated and control db/db mice. Representative image of PARylated PGC-1α in WAT in control ( p ) and G007-LK-treated ( q ) db/db mice. PLA performed with either anti-PGC-1α IgG ( r ) or anti-PAR IgG ( s ) alone. Quantification of PGC-1α PARylation level ( t ). Scale bar, 50 µm. Error bars represent ±SEM. Two-tailed t -test. * p < 0.05. In muscle ( a – j ) n = 3 in each group, in WAT ( k – t ) n = 4 for each group.

Article Snippet: Pull-down assay with WWE Affinity Resin Kit (Tulip Biolabs, West Point, PA, USA) was performed according to the manufacturer’s instruction.

Techniques: Immunoprecipitation, Control, In Situ, Pull Down Assay, Western Blot, Negative Control, Mutagenesis, Binding Assay, Two Tailed Test