replication Search Results


pin multi blot replicator  (V&P Scientific)
92
V&P Scientific pin multi blot replicator
Pin Multi Blot Replicator, supplied by V&P Scientific, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pin multi blot replicator/product/V&P Scientific
Average 92 stars, based on 1 article reviews
pin multi blot replicator - by Bioz Stars, 2026-06
92/100 stars
  Buy from Supplier
anti rpa2  (Proteintech)
93
Proteintech anti rpa2
MiR-139-3p targets <t>RPA2</t> to enhance DNA damage sensitivity in breast cancer cells. (A) Venn diagram of the miR-139-3p target genes and DSB response genes. (B) The relative messenger RNA level of five potential targets in the miR-139-3p in MDA-MB-231 and MCF-7 cells transfected with NC or miR-139-3p mimics. (C) Western blot of RPA2 in the cells transfected with NC or miR-139-3p mimics. (D) The relative Firefly/Renilla luciferase activity in the 293T cells transfected with the luciferase vector containing wt or mut RPA2 3’UTR luciferase plasmids, and NC or miR-139-3p mimics. (E) Stable overexpression of RPA2 -3Flag in BC cells. (F,G) IR colony formation ability of the (F) MDA-MB-231 and (G) MCF-7 cells stably overexpressing RPA2 and transfected with miR-139-3p mimics and stained with crystal violet. 1× magnification. (H,I) Cisplatin sensitivity MTS assays of the (H) MDA-MB-231 and (I) MCF-7 cells in different groups. (J) Schematic of HR repair reporter assays (upper). The HR repair efficiency of the miR-139-3p overexpression group and the co-overexpression of the RPA2 group were measured by flow cytometry, with siBRCA1 as a positive control. (K) Schematic of NHEJ reporter assays (upper). The NHEJ efficiency of the miR-139-3p overexpression group and the co-overexpression of the RPA2 group were measured by flow cytometry, with si53BP1 as a positive control. (L) gH2AX foci immunostained for gH2AX (green) and counterstained with DAPI (blue) at 12 hours post-IR in the MDA-MB-231 cells. The gH2AX foci numbers were counted from a minimum of 150 cells. Scale bar: 10 μm. (M) RAD51 foci in the S phase immunostained for RAD51 (red) and counterstained with DAPI (blue) at 12 hours post-IR in the MDA-MB-231 cells. RAD51 foci numbers were counted from a minimum of 150 cells. Scale bar: 10 mm. ns, non-significant; *, P<0.05; **, P<0.01; ***, P<0.001. DAPI, 4’,6-diamidino-2-phenylindole; DSB, double-strand break; GEP, gene editing plasmid; GFP, green fluorescent protein; gH2AX, phosphorylated H2AX; HR, homologous recombination; iGFP, internal GFP reporter; IR, irradiation; mut, mutant type; NC, negative control; NHEJ, non-homologous end joining; wt, wild type; UTR, untranslated region.
Anti Rpa2, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti rpa2/product/Proteintech
Average 93 stars, based on 1 article reviews
anti rpa2 - by Bioz Stars, 2026-06
93/100 stars
  Buy from Supplier
neuropilin 1  (Boster Bio)
90
Boster Bio neuropilin 1
MiR-139-3p targets <t>RPA2</t> to enhance DNA damage sensitivity in breast cancer cells. (A) Venn diagram of the miR-139-3p target genes and DSB response genes. (B) The relative messenger RNA level of five potential targets in the miR-139-3p in MDA-MB-231 and MCF-7 cells transfected with NC or miR-139-3p mimics. (C) Western blot of RPA2 in the cells transfected with NC or miR-139-3p mimics. (D) The relative Firefly/Renilla luciferase activity in the 293T cells transfected with the luciferase vector containing wt or mut RPA2 3’UTR luciferase plasmids, and NC or miR-139-3p mimics. (E) Stable overexpression of RPA2 -3Flag in BC cells. (F,G) IR colony formation ability of the (F) MDA-MB-231 and (G) MCF-7 cells stably overexpressing RPA2 and transfected with miR-139-3p mimics and stained with crystal violet. 1× magnification. (H,I) Cisplatin sensitivity MTS assays of the (H) MDA-MB-231 and (I) MCF-7 cells in different groups. (J) Schematic of HR repair reporter assays (upper). The HR repair efficiency of the miR-139-3p overexpression group and the co-overexpression of the RPA2 group were measured by flow cytometry, with siBRCA1 as a positive control. (K) Schematic of NHEJ reporter assays (upper). The NHEJ efficiency of the miR-139-3p overexpression group and the co-overexpression of the RPA2 group were measured by flow cytometry, with si53BP1 as a positive control. (L) gH2AX foci immunostained for gH2AX (green) and counterstained with DAPI (blue) at 12 hours post-IR in the MDA-MB-231 cells. The gH2AX foci numbers were counted from a minimum of 150 cells. Scale bar: 10 μm. (M) RAD51 foci in the S phase immunostained for RAD51 (red) and counterstained with DAPI (blue) at 12 hours post-IR in the MDA-MB-231 cells. RAD51 foci numbers were counted from a minimum of 150 cells. Scale bar: 10 mm. ns, non-significant; *, P<0.05; **, P<0.01; ***, P<0.001. DAPI, 4’,6-diamidino-2-phenylindole; DSB, double-strand break; GEP, gene editing plasmid; GFP, green fluorescent protein; gH2AX, phosphorylated H2AX; HR, homologous recombination; iGFP, internal GFP reporter; IR, irradiation; mut, mutant type; NC, negative control; NHEJ, non-homologous end joining; wt, wild type; UTR, untranslated region.
Neuropilin 1, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/neuropilin 1/product/Boster Bio
Average 90 stars, based on 1 article reviews
neuropilin 1 - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
13482 1 ap  (Proteintech)
94
Proteintech 13482 1 ap
MiR-139-3p targets <t>RPA2</t> to enhance DNA damage sensitivity in breast cancer cells. (A) Venn diagram of the miR-139-3p target genes and DSB response genes. (B) The relative messenger RNA level of five potential targets in the miR-139-3p in MDA-MB-231 and MCF-7 cells transfected with NC or miR-139-3p mimics. (C) Western blot of RPA2 in the cells transfected with NC or miR-139-3p mimics. (D) The relative Firefly/Renilla luciferase activity in the 293T cells transfected with the luciferase vector containing wt or mut RPA2 3’UTR luciferase plasmids, and NC or miR-139-3p mimics. (E) Stable overexpression of RPA2 -3Flag in BC cells. (F,G) IR colony formation ability of the (F) MDA-MB-231 and (G) MCF-7 cells stably overexpressing RPA2 and transfected with miR-139-3p mimics and stained with crystal violet. 1× magnification. (H,I) Cisplatin sensitivity MTS assays of the (H) MDA-MB-231 and (I) MCF-7 cells in different groups. (J) Schematic of HR repair reporter assays (upper). The HR repair efficiency of the miR-139-3p overexpression group and the co-overexpression of the RPA2 group were measured by flow cytometry, with siBRCA1 as a positive control. (K) Schematic of NHEJ reporter assays (upper). The NHEJ efficiency of the miR-139-3p overexpression group and the co-overexpression of the RPA2 group were measured by flow cytometry, with si53BP1 as a positive control. (L) gH2AX foci immunostained for gH2AX (green) and counterstained with DAPI (blue) at 12 hours post-IR in the MDA-MB-231 cells. The gH2AX foci numbers were counted from a minimum of 150 cells. Scale bar: 10 μm. (M) RAD51 foci in the S phase immunostained for RAD51 (red) and counterstained with DAPI (blue) at 12 hours post-IR in the MDA-MB-231 cells. RAD51 foci numbers were counted from a minimum of 150 cells. Scale bar: 10 mm. ns, non-significant; *, P<0.05; **, P<0.01; ***, P<0.001. DAPI, 4’,6-diamidino-2-phenylindole; DSB, double-strand break; GEP, gene editing plasmid; GFP, green fluorescent protein; gH2AX, phosphorylated H2AX; HR, homologous recombination; iGFP, internal GFP reporter; IR, irradiation; mut, mutant type; NC, negative control; NHEJ, non-homologous end joining; wt, wild type; UTR, untranslated region.
13482 1 Ap, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/13482 1 ap/product/Proteintech
Average 94 stars, based on 1 article reviews
13482 1 ap - by Bioz Stars, 2026-06
94/100 stars
  Buy from Supplier
rpa70 mouse santa cruz sc  (Proteintech)
93
Proteintech rpa70 mouse santa cruz sc
MiR-139-3p targets <t>RPA2</t> to enhance DNA damage sensitivity in breast cancer cells. (A) Venn diagram of the miR-139-3p target genes and DSB response genes. (B) The relative messenger RNA level of five potential targets in the miR-139-3p in MDA-MB-231 and MCF-7 cells transfected with NC or miR-139-3p mimics. (C) Western blot of RPA2 in the cells transfected with NC or miR-139-3p mimics. (D) The relative Firefly/Renilla luciferase activity in the 293T cells transfected with the luciferase vector containing wt or mut RPA2 3’UTR luciferase plasmids, and NC or miR-139-3p mimics. (E) Stable overexpression of RPA2 -3Flag in BC cells. (F,G) IR colony formation ability of the (F) MDA-MB-231 and (G) MCF-7 cells stably overexpressing RPA2 and transfected with miR-139-3p mimics and stained with crystal violet. 1× magnification. (H,I) Cisplatin sensitivity MTS assays of the (H) MDA-MB-231 and (I) MCF-7 cells in different groups. (J) Schematic of HR repair reporter assays (upper). The HR repair efficiency of the miR-139-3p overexpression group and the co-overexpression of the RPA2 group were measured by flow cytometry, with siBRCA1 as a positive control. (K) Schematic of NHEJ reporter assays (upper). The NHEJ efficiency of the miR-139-3p overexpression group and the co-overexpression of the RPA2 group were measured by flow cytometry, with si53BP1 as a positive control. (L) gH2AX foci immunostained for gH2AX (green) and counterstained with DAPI (blue) at 12 hours post-IR in the MDA-MB-231 cells. The gH2AX foci numbers were counted from a minimum of 150 cells. Scale bar: 10 μm. (M) RAD51 foci in the S phase immunostained for RAD51 (red) and counterstained with DAPI (blue) at 12 hours post-IR in the MDA-MB-231 cells. RAD51 foci numbers were counted from a minimum of 150 cells. Scale bar: 10 mm. ns, non-significant; *, P<0.05; **, P<0.01; ***, P<0.001. DAPI, 4’,6-diamidino-2-phenylindole; DSB, double-strand break; GEP, gene editing plasmid; GFP, green fluorescent protein; gH2AX, phosphorylated H2AX; HR, homologous recombination; iGFP, internal GFP reporter; IR, irradiation; mut, mutant type; NC, negative control; NHEJ, non-homologous end joining; wt, wild type; UTR, untranslated region.
Rpa70 Mouse Santa Cruz Sc, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rpa70 mouse santa cruz sc/product/Proteintech
Average 93 stars, based on 1 article reviews
rpa70 mouse santa cruz sc - by Bioz Stars, 2026-06
93/100 stars
  Buy from Supplier
novus cat nbp2 30645  (Novus Biologicals)
92
Novus Biologicals novus cat nbp2 30645
MiR-139-3p targets <t>RPA2</t> to enhance DNA damage sensitivity in breast cancer cells. (A) Venn diagram of the miR-139-3p target genes and DSB response genes. (B) The relative messenger RNA level of five potential targets in the miR-139-3p in MDA-MB-231 and MCF-7 cells transfected with NC or miR-139-3p mimics. (C) Western blot of RPA2 in the cells transfected with NC or miR-139-3p mimics. (D) The relative Firefly/Renilla luciferase activity in the 293T cells transfected with the luciferase vector containing wt or mut RPA2 3’UTR luciferase plasmids, and NC or miR-139-3p mimics. (E) Stable overexpression of RPA2 -3Flag in BC cells. (F,G) IR colony formation ability of the (F) MDA-MB-231 and (G) MCF-7 cells stably overexpressing RPA2 and transfected with miR-139-3p mimics and stained with crystal violet. 1× magnification. (H,I) Cisplatin sensitivity MTS assays of the (H) MDA-MB-231 and (I) MCF-7 cells in different groups. (J) Schematic of HR repair reporter assays (upper). The HR repair efficiency of the miR-139-3p overexpression group and the co-overexpression of the RPA2 group were measured by flow cytometry, with siBRCA1 as a positive control. (K) Schematic of NHEJ reporter assays (upper). The NHEJ efficiency of the miR-139-3p overexpression group and the co-overexpression of the RPA2 group were measured by flow cytometry, with si53BP1 as a positive control. (L) gH2AX foci immunostained for gH2AX (green) and counterstained with DAPI (blue) at 12 hours post-IR in the MDA-MB-231 cells. The gH2AX foci numbers were counted from a minimum of 150 cells. Scale bar: 10 μm. (M) RAD51 foci in the S phase immunostained for RAD51 (red) and counterstained with DAPI (blue) at 12 hours post-IR in the MDA-MB-231 cells. RAD51 foci numbers were counted from a minimum of 150 cells. Scale bar: 10 mm. ns, non-significant; *, P<0.05; **, P<0.01; ***, P<0.001. DAPI, 4’,6-diamidino-2-phenylindole; DSB, double-strand break; GEP, gene editing plasmid; GFP, green fluorescent protein; gH2AX, phosphorylated H2AX; HR, homologous recombination; iGFP, internal GFP reporter; IR, irradiation; mut, mutant type; NC, negative control; NHEJ, non-homologous end joining; wt, wild type; UTR, untranslated region.
Novus Cat Nbp2 30645, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/novus cat nbp2 30645/product/Novus Biologicals
Average 92 stars, based on 1 article reviews
novus cat nbp2 30645 - by Bioz Stars, 2026-06
92/100 stars
  Buy from Supplier
anti rfc2  (Proteintech)
93
Proteintech anti rfc2
MiR-139-3p targets <t>RPA2</t> to enhance DNA damage sensitivity in breast cancer cells. (A) Venn diagram of the miR-139-3p target genes and DSB response genes. (B) The relative messenger RNA level of five potential targets in the miR-139-3p in MDA-MB-231 and MCF-7 cells transfected with NC or miR-139-3p mimics. (C) Western blot of RPA2 in the cells transfected with NC or miR-139-3p mimics. (D) The relative Firefly/Renilla luciferase activity in the 293T cells transfected with the luciferase vector containing wt or mut RPA2 3’UTR luciferase plasmids, and NC or miR-139-3p mimics. (E) Stable overexpression of RPA2 -3Flag in BC cells. (F,G) IR colony formation ability of the (F) MDA-MB-231 and (G) MCF-7 cells stably overexpressing RPA2 and transfected with miR-139-3p mimics and stained with crystal violet. 1× magnification. (H,I) Cisplatin sensitivity MTS assays of the (H) MDA-MB-231 and (I) MCF-7 cells in different groups. (J) Schematic of HR repair reporter assays (upper). The HR repair efficiency of the miR-139-3p overexpression group and the co-overexpression of the RPA2 group were measured by flow cytometry, with siBRCA1 as a positive control. (K) Schematic of NHEJ reporter assays (upper). The NHEJ efficiency of the miR-139-3p overexpression group and the co-overexpression of the RPA2 group were measured by flow cytometry, with si53BP1 as a positive control. (L) gH2AX foci immunostained for gH2AX (green) and counterstained with DAPI (blue) at 12 hours post-IR in the MDA-MB-231 cells. The gH2AX foci numbers were counted from a minimum of 150 cells. Scale bar: 10 μm. (M) RAD51 foci in the S phase immunostained for RAD51 (red) and counterstained with DAPI (blue) at 12 hours post-IR in the MDA-MB-231 cells. RAD51 foci numbers were counted from a minimum of 150 cells. Scale bar: 10 mm. ns, non-significant; *, P<0.05; **, P<0.01; ***, P<0.001. DAPI, 4’,6-diamidino-2-phenylindole; DSB, double-strand break; GEP, gene editing plasmid; GFP, green fluorescent protein; gH2AX, phosphorylated H2AX; HR, homologous recombination; iGFP, internal GFP reporter; IR, irradiation; mut, mutant type; NC, negative control; NHEJ, non-homologous end joining; wt, wild type; UTR, untranslated region.
Anti Rfc2, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti rfc2/product/Proteintech
Average 93 stars, based on 1 article reviews
anti rfc2 - by Bioz Stars, 2026-06
93/100 stars
  Buy from Supplier
total ticrr flow cytometry  (Rockland Immunochemicals)
90
Rockland Immunochemicals total ticrr flow cytometry
MiR-139-3p targets <t>RPA2</t> to enhance DNA damage sensitivity in breast cancer cells. (A) Venn diagram of the miR-139-3p target genes and DSB response genes. (B) The relative messenger RNA level of five potential targets in the miR-139-3p in MDA-MB-231 and MCF-7 cells transfected with NC or miR-139-3p mimics. (C) Western blot of RPA2 in the cells transfected with NC or miR-139-3p mimics. (D) The relative Firefly/Renilla luciferase activity in the 293T cells transfected with the luciferase vector containing wt or mut RPA2 3’UTR luciferase plasmids, and NC or miR-139-3p mimics. (E) Stable overexpression of RPA2 -3Flag in BC cells. (F,G) IR colony formation ability of the (F) MDA-MB-231 and (G) MCF-7 cells stably overexpressing RPA2 and transfected with miR-139-3p mimics and stained with crystal violet. 1× magnification. (H,I) Cisplatin sensitivity MTS assays of the (H) MDA-MB-231 and (I) MCF-7 cells in different groups. (J) Schematic of HR repair reporter assays (upper). The HR repair efficiency of the miR-139-3p overexpression group and the co-overexpression of the RPA2 group were measured by flow cytometry, with siBRCA1 as a positive control. (K) Schematic of NHEJ reporter assays (upper). The NHEJ efficiency of the miR-139-3p overexpression group and the co-overexpression of the RPA2 group were measured by flow cytometry, with si53BP1 as a positive control. (L) gH2AX foci immunostained for gH2AX (green) and counterstained with DAPI (blue) at 12 hours post-IR in the MDA-MB-231 cells. The gH2AX foci numbers were counted from a minimum of 150 cells. Scale bar: 10 μm. (M) RAD51 foci in the S phase immunostained for RAD51 (red) and counterstained with DAPI (blue) at 12 hours post-IR in the MDA-MB-231 cells. RAD51 foci numbers were counted from a minimum of 150 cells. Scale bar: 10 mm. ns, non-significant; *, P<0.05; **, P<0.01; ***, P<0.001. DAPI, 4’,6-diamidino-2-phenylindole; DSB, double-strand break; GEP, gene editing plasmid; GFP, green fluorescent protein; gH2AX, phosphorylated H2AX; HR, homologous recombination; iGFP, internal GFP reporter; IR, irradiation; mut, mutant type; NC, negative control; NHEJ, non-homologous end joining; wt, wild type; UTR, untranslated region.
Total Ticrr Flow Cytometry, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/total ticrr flow cytometry/product/Rockland Immunochemicals
Average 90 stars, based on 1 article reviews
total ticrr flow cytometry - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
rabbit polyclonal antibodies against cdt1  (Proteintech)
93
Proteintech rabbit polyclonal antibodies against cdt1
MiR-139-3p targets <t>RPA2</t> to enhance DNA damage sensitivity in breast cancer cells. (A) Venn diagram of the miR-139-3p target genes and DSB response genes. (B) The relative messenger RNA level of five potential targets in the miR-139-3p in MDA-MB-231 and MCF-7 cells transfected with NC or miR-139-3p mimics. (C) Western blot of RPA2 in the cells transfected with NC or miR-139-3p mimics. (D) The relative Firefly/Renilla luciferase activity in the 293T cells transfected with the luciferase vector containing wt or mut RPA2 3’UTR luciferase plasmids, and NC or miR-139-3p mimics. (E) Stable overexpression of RPA2 -3Flag in BC cells. (F,G) IR colony formation ability of the (F) MDA-MB-231 and (G) MCF-7 cells stably overexpressing RPA2 and transfected with miR-139-3p mimics and stained with crystal violet. 1× magnification. (H,I) Cisplatin sensitivity MTS assays of the (H) MDA-MB-231 and (I) MCF-7 cells in different groups. (J) Schematic of HR repair reporter assays (upper). The HR repair efficiency of the miR-139-3p overexpression group and the co-overexpression of the RPA2 group were measured by flow cytometry, with siBRCA1 as a positive control. (K) Schematic of NHEJ reporter assays (upper). The NHEJ efficiency of the miR-139-3p overexpression group and the co-overexpression of the RPA2 group were measured by flow cytometry, with si53BP1 as a positive control. (L) gH2AX foci immunostained for gH2AX (green) and counterstained with DAPI (blue) at 12 hours post-IR in the MDA-MB-231 cells. The gH2AX foci numbers were counted from a minimum of 150 cells. Scale bar: 10 μm. (M) RAD51 foci in the S phase immunostained for RAD51 (red) and counterstained with DAPI (blue) at 12 hours post-IR in the MDA-MB-231 cells. RAD51 foci numbers were counted from a minimum of 150 cells. Scale bar: 10 mm. ns, non-significant; *, P<0.05; **, P<0.01; ***, P<0.001. DAPI, 4’,6-diamidino-2-phenylindole; DSB, double-strand break; GEP, gene editing plasmid; GFP, green fluorescent protein; gH2AX, phosphorylated H2AX; HR, homologous recombination; iGFP, internal GFP reporter; IR, irradiation; mut, mutant type; NC, negative control; NHEJ, non-homologous end joining; wt, wild type; UTR, untranslated region.
Rabbit Polyclonal Antibodies Against Cdt1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit polyclonal antibodies against cdt1/product/Proteintech
Average 93 stars, based on 1 article reviews
rabbit polyclonal antibodies against cdt1 - by Bioz Stars, 2026-06
93/100 stars
  Buy from Supplier
multi blot replicator  (V&P Scientific)
94
V&P Scientific multi blot replicator
MiR-139-3p targets <t>RPA2</t> to enhance DNA damage sensitivity in breast cancer cells. (A) Venn diagram of the miR-139-3p target genes and DSB response genes. (B) The relative messenger RNA level of five potential targets in the miR-139-3p in MDA-MB-231 and MCF-7 cells transfected with NC or miR-139-3p mimics. (C) Western blot of RPA2 in the cells transfected with NC or miR-139-3p mimics. (D) The relative Firefly/Renilla luciferase activity in the 293T cells transfected with the luciferase vector containing wt or mut RPA2 3’UTR luciferase plasmids, and NC or miR-139-3p mimics. (E) Stable overexpression of RPA2 -3Flag in BC cells. (F,G) IR colony formation ability of the (F) MDA-MB-231 and (G) MCF-7 cells stably overexpressing RPA2 and transfected with miR-139-3p mimics and stained with crystal violet. 1× magnification. (H,I) Cisplatin sensitivity MTS assays of the (H) MDA-MB-231 and (I) MCF-7 cells in different groups. (J) Schematic of HR repair reporter assays (upper). The HR repair efficiency of the miR-139-3p overexpression group and the co-overexpression of the RPA2 group were measured by flow cytometry, with siBRCA1 as a positive control. (K) Schematic of NHEJ reporter assays (upper). The NHEJ efficiency of the miR-139-3p overexpression group and the co-overexpression of the RPA2 group were measured by flow cytometry, with si53BP1 as a positive control. (L) gH2AX foci immunostained for gH2AX (green) and counterstained with DAPI (blue) at 12 hours post-IR in the MDA-MB-231 cells. The gH2AX foci numbers were counted from a minimum of 150 cells. Scale bar: 10 μm. (M) RAD51 foci in the S phase immunostained for RAD51 (red) and counterstained with DAPI (blue) at 12 hours post-IR in the MDA-MB-231 cells. RAD51 foci numbers were counted from a minimum of 150 cells. Scale bar: 10 mm. ns, non-significant; *, P<0.05; **, P<0.01; ***, P<0.001. DAPI, 4’,6-diamidino-2-phenylindole; DSB, double-strand break; GEP, gene editing plasmid; GFP, green fluorescent protein; gH2AX, phosphorylated H2AX; HR, homologous recombination; iGFP, internal GFP reporter; IR, irradiation; mut, mutant type; NC, negative control; NHEJ, non-homologous end joining; wt, wild type; UTR, untranslated region.
Multi Blot Replicator, supplied by V&P Scientific, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/multi blot replicator/product/V&P Scientific
Average 94 stars, based on 1 article reviews
multi blot replicator - by Bioz Stars, 2026-06
94/100 stars
  Buy from Supplier
replication cohort for rs12483205  (Thermo Fisher)
91
Thermo Fisher replication cohort for rs12483205
SNPs ( p <5×10 −5 ) associated with in vitro HIV-1 replication in monocyte-derived macrophages.
Replication Cohort For Rs12483205, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/replication cohort for rs12483205/product/Thermo Fisher
Average 91 stars, based on 1 article reviews
replication cohort for rs12483205 - by Bioz Stars, 2026-06
91/100 stars
  Buy from Supplier
anti rfc3  (Proteintech)
92
Proteintech anti rfc3
SNPs ( p <5×10 −5 ) associated with in vitro HIV-1 replication in monocyte-derived macrophages.
Anti Rfc3, supplied by Proteintech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti rfc3/product/Proteintech
Average 92 stars, based on 1 article reviews
anti rfc3 - by Bioz Stars, 2026-06
92/100 stars
  Buy from Supplier

Image Search Results


MiR-139-3p targets RPA2 to enhance DNA damage sensitivity in breast cancer cells. (A) Venn diagram of the miR-139-3p target genes and DSB response genes. (B) The relative messenger RNA level of five potential targets in the miR-139-3p in MDA-MB-231 and MCF-7 cells transfected with NC or miR-139-3p mimics. (C) Western blot of RPA2 in the cells transfected with NC or miR-139-3p mimics. (D) The relative Firefly/Renilla luciferase activity in the 293T cells transfected with the luciferase vector containing wt or mut RPA2 3’UTR luciferase plasmids, and NC or miR-139-3p mimics. (E) Stable overexpression of RPA2 -3Flag in BC cells. (F,G) IR colony formation ability of the (F) MDA-MB-231 and (G) MCF-7 cells stably overexpressing RPA2 and transfected with miR-139-3p mimics and stained with crystal violet. 1× magnification. (H,I) Cisplatin sensitivity MTS assays of the (H) MDA-MB-231 and (I) MCF-7 cells in different groups. (J) Schematic of HR repair reporter assays (upper). The HR repair efficiency of the miR-139-3p overexpression group and the co-overexpression of the RPA2 group were measured by flow cytometry, with siBRCA1 as a positive control. (K) Schematic of NHEJ reporter assays (upper). The NHEJ efficiency of the miR-139-3p overexpression group and the co-overexpression of the RPA2 group were measured by flow cytometry, with si53BP1 as a positive control. (L) gH2AX foci immunostained for gH2AX (green) and counterstained with DAPI (blue) at 12 hours post-IR in the MDA-MB-231 cells. The gH2AX foci numbers were counted from a minimum of 150 cells. Scale bar: 10 μm. (M) RAD51 foci in the S phase immunostained for RAD51 (red) and counterstained with DAPI (blue) at 12 hours post-IR in the MDA-MB-231 cells. RAD51 foci numbers were counted from a minimum of 150 cells. Scale bar: 10 mm. ns, non-significant; *, P<0.05; **, P<0.01; ***, P<0.001. DAPI, 4’,6-diamidino-2-phenylindole; DSB, double-strand break; GEP, gene editing plasmid; GFP, green fluorescent protein; gH2AX, phosphorylated H2AX; HR, homologous recombination; iGFP, internal GFP reporter; IR, irradiation; mut, mutant type; NC, negative control; NHEJ, non-homologous end joining; wt, wild type; UTR, untranslated region.

Journal: Translational Cancer Research

Article Title: A circulating microRNA-based diagnostic model for breast cancer, in which the miR-139-3p/RPA2 axis regulates the sensitivity to DNA-damaging agents

doi: 10.21037/tcr-2025-1-2592

Figure Lengend Snippet: MiR-139-3p targets RPA2 to enhance DNA damage sensitivity in breast cancer cells. (A) Venn diagram of the miR-139-3p target genes and DSB response genes. (B) The relative messenger RNA level of five potential targets in the miR-139-3p in MDA-MB-231 and MCF-7 cells transfected with NC or miR-139-3p mimics. (C) Western blot of RPA2 in the cells transfected with NC or miR-139-3p mimics. (D) The relative Firefly/Renilla luciferase activity in the 293T cells transfected with the luciferase vector containing wt or mut RPA2 3’UTR luciferase plasmids, and NC or miR-139-3p mimics. (E) Stable overexpression of RPA2 -3Flag in BC cells. (F,G) IR colony formation ability of the (F) MDA-MB-231 and (G) MCF-7 cells stably overexpressing RPA2 and transfected with miR-139-3p mimics and stained with crystal violet. 1× magnification. (H,I) Cisplatin sensitivity MTS assays of the (H) MDA-MB-231 and (I) MCF-7 cells in different groups. (J) Schematic of HR repair reporter assays (upper). The HR repair efficiency of the miR-139-3p overexpression group and the co-overexpression of the RPA2 group were measured by flow cytometry, with siBRCA1 as a positive control. (K) Schematic of NHEJ reporter assays (upper). The NHEJ efficiency of the miR-139-3p overexpression group and the co-overexpression of the RPA2 group were measured by flow cytometry, with si53BP1 as a positive control. (L) gH2AX foci immunostained for gH2AX (green) and counterstained with DAPI (blue) at 12 hours post-IR in the MDA-MB-231 cells. The gH2AX foci numbers were counted from a minimum of 150 cells. Scale bar: 10 μm. (M) RAD51 foci in the S phase immunostained for RAD51 (red) and counterstained with DAPI (blue) at 12 hours post-IR in the MDA-MB-231 cells. RAD51 foci numbers were counted from a minimum of 150 cells. Scale bar: 10 mm. ns, non-significant; *, P<0.05; **, P<0.01; ***, P<0.001. DAPI, 4’,6-diamidino-2-phenylindole; DSB, double-strand break; GEP, gene editing plasmid; GFP, green fluorescent protein; gH2AX, phosphorylated H2AX; HR, homologous recombination; iGFP, internal GFP reporter; IR, irradiation; mut, mutant type; NC, negative control; NHEJ, non-homologous end joining; wt, wild type; UTR, untranslated region.

Article Snippet: The following antibodies were used: anti- gH2AX (Millipore, Massachusetts, USA, 05-636), anti-β-actin (Servicebio, Wuhan, China, GB12001), anti- RPA2 (ProteinTech, Wuhan, China, 10412-1-AP).

Techniques: Transfection, Western Blot, Luciferase, Activity Assay, Plasmid Preparation, Over Expression, Stable Transfection, Staining, Flow Cytometry, Positive Control, Homologous Recombination, Irradiation, Mutagenesis, Negative Control, Non-Homologous End Joining

The efficacy of the combination of miR-139-3p and cisplatin in vivo . (A) Schematic diagram of the construction and treatment of the xenograft model in the BALB/c nude mice. (B) Representative images of nude mice with xenograft tumors in each group. (C) Growth curve of xenograft tumors in nude mice from different groups. (D) IHC staining and analysis of the RPA2 protein in xenograft tumor. 20× magnification. (E) IHC staining and analysis of the Ki-67 protein in xenograft tumors. 1× magnification. *, P<0.05; **, P<0.01; ***, P<0.001. IHC, immunohistochemistry; NC, negative control; PBS, phosphate-buffered saline.

Journal: Translational Cancer Research

Article Title: A circulating microRNA-based diagnostic model for breast cancer, in which the miR-139-3p/RPA2 axis regulates the sensitivity to DNA-damaging agents

doi: 10.21037/tcr-2025-1-2592

Figure Lengend Snippet: The efficacy of the combination of miR-139-3p and cisplatin in vivo . (A) Schematic diagram of the construction and treatment of the xenograft model in the BALB/c nude mice. (B) Representative images of nude mice with xenograft tumors in each group. (C) Growth curve of xenograft tumors in nude mice from different groups. (D) IHC staining and analysis of the RPA2 protein in xenograft tumor. 20× magnification. (E) IHC staining and analysis of the Ki-67 protein in xenograft tumors. 1× magnification. *, P<0.05; **, P<0.01; ***, P<0.001. IHC, immunohistochemistry; NC, negative control; PBS, phosphate-buffered saline.

Article Snippet: The following antibodies were used: anti- gH2AX (Millipore, Massachusetts, USA, 05-636), anti-β-actin (Servicebio, Wuhan, China, GB12001), anti- RPA2 (ProteinTech, Wuhan, China, 10412-1-AP).

Techniques: In Vivo, Immunohistochemistry, Negative Control, Saline

SNPs ( p <5×10 −5 ) associated with in vitro HIV-1 replication in monocyte-derived macrophages.

Journal: PLoS ONE

Article Title: Genome-Wide Association Study Identifies Single Nucleotide Polymorphism in DYRK1A Associated with Replication of HIV-1 in Monocyte-Derived Macrophages

doi: 10.1371/journal.pone.0017190

Figure Lengend Snippet: SNPs ( p <5×10 −5 ) associated with in vitro HIV-1 replication in monocyte-derived macrophages.

Article Snippet: ABI TaqMan® SNP genotyping assays were used to genotype DNA from the replication cohort for rs12483205 (C_31609775_10), rs12909130 (C_1342209_10) as a proxy for rs2304418, rs2905 (C_3236245_20), rs1046099 (C_7585751_10) and rs17519417 (C_33238869_10) (Applied Biosystems, Carlsbad, CA, USA).

Techniques: In Vitro

The negative association between the rs12483205 minor allele and Gag p24 levels in MDM culture supernatant 14 days after inoculation with HIV-1, was found to match with the results from the genome-wide association study. Open circles represent results from donors with the CCR5 Δ32 wild-type genotype, filled circles from donors with the CCR5 wt/ Δ32 heterozygous genotype. MAJ, homozygous for the major allele; HZ, heterozygote; MIN, homozygous for the minor allele.

Journal: PLoS ONE

Article Title: Genome-Wide Association Study Identifies Single Nucleotide Polymorphism in DYRK1A Associated with Replication of HIV-1 in Monocyte-Derived Macrophages

doi: 10.1371/journal.pone.0017190

Figure Lengend Snippet: The negative association between the rs12483205 minor allele and Gag p24 levels in MDM culture supernatant 14 days after inoculation with HIV-1, was found to match with the results from the genome-wide association study. Open circles represent results from donors with the CCR5 Δ32 wild-type genotype, filled circles from donors with the CCR5 wt/ Δ32 heterozygous genotype. MAJ, homozygous for the major allele; HZ, heterozygote; MIN, homozygous for the minor allele.

Article Snippet: ABI TaqMan® SNP genotyping assays were used to genotype DNA from the replication cohort for rs12483205 (C_31609775_10), rs12909130 (C_1342209_10) as a proxy for rs2304418, rs2905 (C_3236245_20), rs1046099 (C_7585751_10) and rs17519417 (C_33238869_10) (Applied Biosystems, Carlsbad, CA, USA).

Techniques: GWAS

Results ( p values) for association testing using the additive analysis model, between disease progression or viral load and the SNPs in DYRK1A , PDE8A , UBR7 , MOAP1 and SPOCK3 .

Journal: PLoS ONE

Article Title: Genome-Wide Association Study Identifies Single Nucleotide Polymorphism in DYRK1A Associated with Replication of HIV-1 in Monocyte-Derived Macrophages

doi: 10.1371/journal.pone.0017190

Figure Lengend Snippet: Results ( p values) for association testing using the additive analysis model, between disease progression or viral load and the SNPs in DYRK1A , PDE8A , UBR7 , MOAP1 and SPOCK3 .

Article Snippet: ABI TaqMan® SNP genotyping assays were used to genotype DNA from the replication cohort for rs12483205 (C_31609775_10), rs12909130 (C_1342209_10) as a proxy for rs2304418, rs2905 (C_3236245_20), rs1046099 (C_7585751_10) and rs17519417 (C_33238869_10) (Applied Biosystems, Carlsbad, CA, USA).

Techniques: Biomarker Discovery

Untranslated regions (UTR) are shown as open blocks, whereas exons are shown as filled blocks. SNP rs12483205 lies in close proximity to a part of the 5′ UTR unique for splice variant 3.

Journal: PLoS ONE

Article Title: Genome-Wide Association Study Identifies Single Nucleotide Polymorphism in DYRK1A Associated with Replication of HIV-1 in Monocyte-Derived Macrophages

doi: 10.1371/journal.pone.0017190

Figure Lengend Snippet: Untranslated regions (UTR) are shown as open blocks, whereas exons are shown as filled blocks. SNP rs12483205 lies in close proximity to a part of the 5′ UTR unique for splice variant 3.

Article Snippet: ABI TaqMan® SNP genotyping assays were used to genotype DNA from the replication cohort for rs12483205 (C_31609775_10), rs12909130 (C_1342209_10) as a proxy for rs2304418, rs2905 (C_3236245_20), rs1046099 (C_7585751_10) and rs17519417 (C_33238869_10) (Applied Biosystems, Carlsbad, CA, USA).

Techniques: Variant Assay