Journal: Cancers

Article Title: A Double-Negative Feedback Interaction between miR-21 and PPAR-α in Clear Renal Cell Carcinoma

doi: 10.3390/cancers14030795

Figure Lengend Snippet: miR-21 and PPAR-α expressions in HK-2 and renal cancer cell lines before confluence (BC) and two days after confluence (C + 2). ( A ) Using RT-qPCR, miR-21 expression was determined in the 786-O, ACHN, RCC10, and RCC4 renal cancer cells as compared to normal HK-2 renal epithelial cells. RNU48 was used as an internal control. The values are means ± SEM and represent at least three separate experiments (* p < 0.05, ** p < 0.01, and *** p < 0.001). ( B ) Western blotting was performed on whole cell extracts. Antibodies against PPAR-α and β-actin were used.

Article Snippet: The HK-2 human proximal tubule epithelial cell line, ACHN, and 786-O human renal cancer cell lines were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Quantitative RT-PCR, Expressing, Control, Western Blot

Journal: American Journal of Translational Research

Article Title: Individualized screening for chaperone activity in Gaucher disease using multiple patient derived primary cell lines

doi:

Figure Lengend Snippet: Ambroxol increases GCase activity in urine kidney epithelial cells. (A, B) GCase activity in UKEC cells derived from a healthy control (white bar) and a GD patient with genotype L444P/N370S (black bar) cultured for 5 days in presence of increasing concentrations of ambroxol. The data are expressed as % GCase activity compared to respective untreated cells (A) and as GCase concentration nmol/hr/mg protein (B). Each bar represents the average ± sem. *P < 0.05 compared with an untreated group. (C) Q-PCR was performed to determine relative GBA mRNA expression levels after 5 days ambroxol treatment.

Article Snippet: Cells were then plated in a 24-well dish with renal epithelial cell basal media supplemented with renal epithelial cell growth kit (ATCC) and normocin (InvivoGen).

Techniques: Activity Assay, Derivative Assay, Control, Cell Culture, Concentration Assay, Expressing

Journal: Journal of Functional Foods

Article Title: Protective effects of selenium-enriched peptides from Cardamine violifolia on d-galactose-induced brain aging by alleviating oxidative stress, neuroinflammation, and neuron apoptosis

doi: 10.1016/j.jff.2020.104277

Figure Lengend Snippet: Fig. 7. CSP supplementation reduced D-gal-induced overexpression of RAGE, BACE-1, Aβ-42 and PS1. (A) Western blot analysis of RAGE, BACE-1, Aβ-42 and PS1; (B) The relative protein expressions of RAGE, BACE-1, Aβ-42 and PS1; Data are expressed as the mean ± SEM (n = 3). Differences were denoted as follows: * P < 0.05, ** P < 0.01, *** P < 0.001 compared with NC; # P < 0.05, ## p < 0.01, ### p < 0.001 compared with D-gal; & P < 0.05, && p < 0.01, &&& p < 0.001 compared with CSPL.

Article Snippet: Primary antibodies against NFkβ-p65, RAGE, BACE1, PS1, BAX, BCL2, Caspase-3, HO1, NQO1, and β-actin were purchased from Proteintech (Rosemont, IL, USA).

Techniques: Over Expression, Western Blot