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nk92 cell line  (ATCC)
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ATCC nk92 cell line
(A) Flow cytometric analysis of PD-L1, PD-L2, CD47, and CD24 expression in B7-H3–knockdown SNU449 cells. (B) Quantification of MFI shown in (A), expressed as relative MFI (%) compared with siCon cells. (C) Flow cytometric analysis of PD-L1, PD-L2, and CD47 expression in control and B7-H3 KO SNU449 cells. (D) Quantification of MFI shown in (C), expressed as relative MFI (%) compared with control cells. (E) Western blot analysis of immune checkpoint proteins in control and B7-H3 KO SNU449 cells. Protein levels were quantified using ImageJ software with GAPDH as a loading control. (F) <t>NK92</t> cell–mediated cytotoxicity assay. Control and B7-H3 KO SNU449 cells were co-cultured with NK92 cells at a 1:1 effector-to-target (E:T) ratio for 48 hours, followed by crystal violet staining of surviving tumor cells. Representative images from three independent experiments are shown. (G) Quantification of surviving cells shown in (F) by measuring absorbance at 590 nm. (H) NK92 cell–mediated cytotoxicity assessed by calcein-AM release assay at various E:T ratios. (I) Primary NK cell–mediated cytotoxicity assessed by calcein-AM release assay at various E:T ratios. ns, not significant; *, p < 0.05; **, p < 0.01; ***, p < 0.005.
Nk92 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nk92 cell line - by Bioz Stars, 2026-11
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(A) Flow cytometric analysis of PD-L1, PD-L2, CD47, and CD24 expression in B7-H3–knockdown SNU449 cells. (B) Quantification of MFI shown in (A), expressed as relative MFI (%) compared with siCon cells. (C) Flow cytometric analysis of PD-L1, PD-L2, and CD47 expression in control and B7-H3 KO SNU449 cells. (D) Quantification of MFI shown in (C), expressed as relative MFI (%) compared with control cells. (E) Western blot analysis of immune checkpoint proteins in control and B7-H3 KO SNU449 cells. Protein levels were quantified using ImageJ software with GAPDH as a loading control. (F) NK92 cell–mediated cytotoxicity assay. Control and B7-H3 KO SNU449 cells were co-cultured with NK92 cells at a 1:1 effector-to-target (E:T) ratio for 48 hours, followed by crystal violet staining of surviving tumor cells. Representative images from three independent experiments are shown. (G) Quantification of surviving cells shown in (F) by measuring absorbance at 590 nm. (H) NK92 cell–mediated cytotoxicity assessed by calcein-AM release assay at various E:T ratios. (I) Primary NK cell–mediated cytotoxicity assessed by calcein-AM release assay at various E:T ratios. ns, not significant; *, p < 0.05; **, p < 0.01; ***, p < 0.005.

Journal: bioRxiv

Article Title: B7-H3 Modulates Cell Adhesion and Immune Evasion to Promote Tumor Progression and Natural Killer Cell Resistance in Hepatocellular Carcinoma

doi: 10.64898/2026.03.28.714951

Figure Lengend Snippet: (A) Flow cytometric analysis of PD-L1, PD-L2, CD47, and CD24 expression in B7-H3–knockdown SNU449 cells. (B) Quantification of MFI shown in (A), expressed as relative MFI (%) compared with siCon cells. (C) Flow cytometric analysis of PD-L1, PD-L2, and CD47 expression in control and B7-H3 KO SNU449 cells. (D) Quantification of MFI shown in (C), expressed as relative MFI (%) compared with control cells. (E) Western blot analysis of immune checkpoint proteins in control and B7-H3 KO SNU449 cells. Protein levels were quantified using ImageJ software with GAPDH as a loading control. (F) NK92 cell–mediated cytotoxicity assay. Control and B7-H3 KO SNU449 cells were co-cultured with NK92 cells at a 1:1 effector-to-target (E:T) ratio for 48 hours, followed by crystal violet staining of surviving tumor cells. Representative images from three independent experiments are shown. (G) Quantification of surviving cells shown in (F) by measuring absorbance at 590 nm. (H) NK92 cell–mediated cytotoxicity assessed by calcein-AM release assay at various E:T ratios. (I) Primary NK cell–mediated cytotoxicity assessed by calcein-AM release assay at various E:T ratios. ns, not significant; *, p < 0.05; **, p < 0.01; ***, p < 0.005.

Article Snippet: The NK92 cell line was purchased from ATCC and cultured in α-MEM (Biowest) medium supplemented with 12.5% FBS (Biowest), 12.5% horse serum (Biowest), 200μM inositol (Sigma-Aldrich, St.Lois, MO, USA), 20μM Folic acid (Sigma-Aldrich), 100μM 2-mercaptoethanol (Sigma-Aldrich), AA solution (Biowest), and 400 unit/ml IL2 (Peprotech, Rocky Hill, NJ, USA).

Techniques: Expressing, Knockdown, Control, Western Blot, Software, Cytotoxicity Assay, Cell Culture, Staining, Release Assay