Journal: Molecular Metabolism

Article Title: METTL18 ensures pancreatic function by maintaining proper translation and proteostasis

doi: 10.1016/j.molmet.2026.102337

Figure Lengend Snippet: Proteomic alterations in the pancreas of Mettl18 -deficient mice. (A) Volcano plot of quantitative LC-MS/MS of the mouse pancreas. n = 3 per genotype. Proteins with |log2FC| > 0.5 and -Log 10 (P-value) > 4 are considered significantly changed. (B) Gene ontology (GO) analysis. A functional gene annotation of the 19 proteins (up) or 13 proteins (down) was performed with DAVID GO analysis (v6.8). Functional annotations against Biological Process, Molecular Function, and Cellular Component gene ontology with FDR less than 0.05 were shown. (C) Western blot of the pancreatic proteins from WT and KO mice. Top; anti-Reg1 antibody, bottom; anti-β-actin antibody. (D) Quantification of Reg1 protein in the pancreas. 16-week-old; n = 6; mean ± SEM. Student's t -test: p∗∗ = 0.00423. (E) qPCR quantification of mRNA expression of Reg1 in the pancreas. 16-week-old; n = 7; mean ± SEM. Student's t -test: n.s. >0.1. (F) Distribution of Reg1-FLAG. WT and two independent Mettl18 KO clones (KO1 and KO2) of 266-6 cells were transfected with Reg1-FLAG. After 48 h, cells were fractionated into soluble and insoluble fractions, and Reg1-FLAG was analyzed by Western blotting. n = 3; mean ± SEM. Dunnett's test: p∗ <0.05. See also . (G) WT and Mettl18 KO cells were transfected with the plasmid for Reg1-FLAG. 48 h after transfection, cells were fixed, and Reg1-FLAG aggregation was observed under a microscope. Arrowhead indicates aggregation foci. Scale bar: 10 μm. (H) Quantification of 266-6 cells with Reg1-FLAG aggregation. Mean value of three independent experiments was shown. Dunnett's test: p∗∗ = 0.00195, p∗∗∗ = 0.00054. (I) Distribution of Reg1-FLAG in rescued cells. WT and KO1 cells were transfected with Reg1-FLAG together with or without METTL18-WT-HA or its catalytic mutant, METTL18-mut-HA. Forty-eight hours after, cells were fractionated into soluble and insoluble fractions, and Reg1-FLAG was analyzed by Western blotting. n = 3; mean ± SEM. Statistical significance was assessed using Dunnett's test with the KO group as the reference. P∗∗ <0.01. See also . (J) WT and KO1 cells transfected with Reg1-FLAG together with or without METTL18-WT-HA or METTL18-mut-HA were fixed 48 h after transfection, and FLAG signals were visualized by fluorescence microscopy. Scale bar: 10 μm. (K) Quantification of the Reg1-FLAG aggregation. n = 3; mean ± SEM. Dunnett's test: p∗∗ = 0.00498, p∗∗∗<0.0001.

Article Snippet: Other antibodies used were as follows: anti-β-actin (clone 6D1, cat#M177-3; MBL); anti-phospho-PERK (Thr980) (clone G.305.4, cat#MA5-15033; Invitrogen); anti-RPL3 (cat#66130-1-Ig), anti-Reg1 (cat#15850-1-AP), and anti-PERK/EIF2AK3 (cat#24390-1-AP) (Proteintech Group); anti-IRE1 (cat#3294), anti-ATF4 (cat#11815), and anti-eIF5A (cat#20765) (Cell Signaling Technology); anti-phospho-IRE1 (cat#ab48187), anti-phospho-EIF2A (cat#ab32157), and anti-EIF2A (cat#ab5369) (Abcam); anti-IL-1β (cat#AF-401-NA; R&D Systems).

Techniques: Liquid Chromatography with Mass Spectroscopy, Functional Assay, Western Blot, Expressing, Clone Assay, Transfection, Plasmid Preparation, Microscopy, Mutagenesis, Fluorescence

Journal: Micromachines

Article Title: Features in Microfluidic Paper-Based Devices Made by Laser Cutting: How Small Can They Be?

doi: 10.3390/mi9050220

Figure Lengend Snippet: Testing small-scale channels of different width with dye solution to determine the smallest channel width that can be fabricated from a particular paper type. Channels fabricated from RC-55 are shown in the figure. The actual width is listed as N/A for cases where the channel failed to provide a continuous flow path and so no successful width could be listed.

Article Snippet: In this experimental study, we determine the smallest feature sizes that will still enable fluid flow for five different types of paper: (i) Whatman 1 Chr chromatography paper (1 Chr), (ii) Whatman 3MM Chr chromatography paper (3MM Chr), (iii) Whatman regenerated cellulose membrane 55 (RC-55), (iv) Whatman filter paper grade 50 (FP-50), and (v) Amershan Protran 0.45 nitrocellulose membrane (NC).

Techniques:

Journal: Micromachines

Article Title: Features in Microfluidic Paper-Based Devices Made by Laser Cutting: How Small Can They Be?

doi: 10.3390/mi9050220

Figure Lengend Snippet: ( a ) The smallest channel widths in each of the paper types for successful fluid flow plotted against fiber widths. SEM images of the channels generated in the different paper types showing one intact channel with successful fluid flow (on the top) and one narrower channel where fluid flow was not successful (on the bottom), for ( b ) FP-50, ( c ) 3MM Chr, ( d ) 1 Chr, and ( e ) RC-55.

Article Snippet: In this experimental study, we determine the smallest feature sizes that will still enable fluid flow for five different types of paper: (i) Whatman 1 Chr chromatography paper (1 Chr), (ii) Whatman 3MM Chr chromatography paper (3MM Chr), (iii) Whatman regenerated cellulose membrane 55 (RC-55), (iv) Whatman filter paper grade 50 (FP-50), and (v) Amershan Protran 0.45 nitrocellulose membrane (NC).

Techniques: Generated

Journal: Micromachines

Article Title: Features in Microfluidic Paper-Based Devices Made by Laser Cutting: How Small Can They Be?

doi: 10.3390/mi9050220

Figure Lengend Snippet: Flow behavior through varying channel widths fabricated in ( a ) 1 Chr, ( b ) 3MM Chr, and ( c ) RC-55.

Article Snippet: In this experimental study, we determine the smallest feature sizes that will still enable fluid flow for five different types of paper: (i) Whatman 1 Chr chromatography paper (1 Chr), (ii) Whatman 3MM Chr chromatography paper (3MM Chr), (iii) Whatman regenerated cellulose membrane 55 (RC-55), (iv) Whatman filter paper grade 50 (FP-50), and (v) Amershan Protran 0.45 nitrocellulose membrane (NC).

Techniques:

Journal: Micromachines

Article Title: Features in Microfluidic Paper-Based Devices Made by Laser Cutting: How Small Can They Be?

doi: 10.3390/mi9050220

Figure Lengend Snippet: Photographs of the flow of red dye in NC and RC-55. ( a ) Cross-sectional views after red dye flowed through NC and RC-55. ( b ) Overhead view showing the concave flow profile of red dye in NC indicating faster flow along the sides of the channel.

Article Snippet: In this experimental study, we determine the smallest feature sizes that will still enable fluid flow for five different types of paper: (i) Whatman 1 Chr chromatography paper (1 Chr), (ii) Whatman 3MM Chr chromatography paper (3MM Chr), (iii) Whatman regenerated cellulose membrane 55 (RC-55), (iv) Whatman filter paper grade 50 (FP-50), and (v) Amershan Protran 0.45 nitrocellulose membrane (NC).

Techniques: