reference strain vr2332 Search Results


96
ATCC vr2332 na reference strain
Experimental design.
Vr2332 Na Reference Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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95
ATCC reference atcc vr2332
Experimental design.
Reference Atcc Vr2332, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 1 article reviews
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95
ATCC reference strain vr 2332
Experimental design.
Reference Strain Vr 2332, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reference+strain+vr2332/Lactobacillus+johnsonii/pmc09783555-59-2-5
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94
ATCC prrsv 2 reference strains
Experimental design.
Prrsv 2 Reference Strains, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Experimental design.

Journal: Vaccines

Article Title: Broad Protection of Pigs against Heterologous PRRSV Strains by a GP5-Mosaic DNA Vaccine Prime/GP5-Mosaic rVaccinia (VACV) Vaccine Boost

doi: 10.3390/vaccines8010106

Figure Lengend Snippet: Experimental design.

Article Snippet: The viruses used in the study included the VR2332 NA reference strain (ATCC VR-2332) and the MN184C strain provided kindly by Drs. Kelly Lager and Kay Faaberg at U.S. Department of Agriculture-Agriculture Research Service (USDA-ARS).

Techniques:

Phylogenetic analysis of the GP5 Amino acid sequence of the two Mosaic sequences, MN184C and VR2332. The analysis was done via the neighbor-joining method using MEGA7.0.

Journal: Vaccines

Article Title: Broad Protection of Pigs against Heterologous PRRSV Strains by a GP5-Mosaic DNA Vaccine Prime/GP5-Mosaic rVaccinia (VACV) Vaccine Boost

doi: 10.3390/vaccines8010106

Figure Lengend Snippet: Phylogenetic analysis of the GP5 Amino acid sequence of the two Mosaic sequences, MN184C and VR2332. The analysis was done via the neighbor-joining method using MEGA7.0.

Article Snippet: The viruses used in the study included the VR2332 NA reference strain (ATCC VR-2332) and the MN184C strain provided kindly by Drs. Kelly Lager and Kay Faaberg at U.S. Department of Agriculture-Agriculture Research Service (USDA-ARS).

Techniques: Sequencing

Vaccination-induced humoral and cellular responses. ( A ) The virus neutralization was expressed as viral copy numbers (log10 scale), as measured by RT-qPCR in cell supernatants after the infection of MARC-145 cells with pre-incubated serum–virus mixtures. ( B ) The expression of IFN-γ mRNA as fold changes, by either VR2332 or MN184C-stimulated PBMCs collected on the challenge day. Each dot represents the value of one animal. The variation is expressed as standard error of the means. There were three independent replications. Significant differences were calculated by a two-way ANOVA or Student’s t test (* p < 0.05, *** p < 0.0001).

Journal: Vaccines

Article Title: Broad Protection of Pigs against Heterologous PRRSV Strains by a GP5-Mosaic DNA Vaccine Prime/GP5-Mosaic rVaccinia (VACV) Vaccine Boost

doi: 10.3390/vaccines8010106

Figure Lengend Snippet: Vaccination-induced humoral and cellular responses. ( A ) The virus neutralization was expressed as viral copy numbers (log10 scale), as measured by RT-qPCR in cell supernatants after the infection of MARC-145 cells with pre-incubated serum–virus mixtures. ( B ) The expression of IFN-γ mRNA as fold changes, by either VR2332 or MN184C-stimulated PBMCs collected on the challenge day. Each dot represents the value of one animal. The variation is expressed as standard error of the means. There were three independent replications. Significant differences were calculated by a two-way ANOVA or Student’s t test (* p < 0.05, *** p < 0.0001).

Article Snippet: The viruses used in the study included the VR2332 NA reference strain (ATCC VR-2332) and the MN184C strain provided kindly by Drs. Kelly Lager and Kay Faaberg at U.S. Department of Agriculture-Agriculture Research Service (USDA-ARS).

Techniques: Virus, Neutralization, Quantitative RT-PCR, Infection, Incubation, Expressing

Virus clearance in sera and tissues. ( A ) Viral copy numbers in serum from 0 to 14 DPC upon VR2332 challenge. ( B ) The viral copy numbers in serum from 0 to 14 DPC upon MN184C challenge. Each dot represents the mean value of each group. The variation bars are expressed as the standard error of the mean. Three separate experiments were performed for each. A two-way ANOVA or Student’s t test (* p < 0.05, ** p < 0.01, was used to calculate significant differences; days post-challenge (DPC). ( C ) The viral copy numbers in tissues at necropsy upon VR2332 challenge. ( D ) The viral copy numbers in tissues at necropsy upon MN184C challenge. Each bar represents the mean value of each group. The bars are the standard error of the mean. Three separate experiments were performed for each. Significant differences were calculated by Student’s t test (* p < 0.05).

Journal: Vaccines

Article Title: Broad Protection of Pigs against Heterologous PRRSV Strains by a GP5-Mosaic DNA Vaccine Prime/GP5-Mosaic rVaccinia (VACV) Vaccine Boost

doi: 10.3390/vaccines8010106

Figure Lengend Snippet: Virus clearance in sera and tissues. ( A ) Viral copy numbers in serum from 0 to 14 DPC upon VR2332 challenge. ( B ) The viral copy numbers in serum from 0 to 14 DPC upon MN184C challenge. Each dot represents the mean value of each group. The variation bars are expressed as the standard error of the mean. Three separate experiments were performed for each. A two-way ANOVA or Student’s t test (* p < 0.05, ** p < 0.01, was used to calculate significant differences; days post-challenge (DPC). ( C ) The viral copy numbers in tissues at necropsy upon VR2332 challenge. ( D ) The viral copy numbers in tissues at necropsy upon MN184C challenge. Each bar represents the mean value of each group. The bars are the standard error of the mean. Three separate experiments were performed for each. Significant differences were calculated by Student’s t test (* p < 0.05).

Article Snippet: The viruses used in the study included the VR2332 NA reference strain (ATCC VR-2332) and the MN184C strain provided kindly by Drs. Kelly Lager and Kay Faaberg at U.S. Department of Agriculture-Agriculture Research Service (USDA-ARS).

Techniques: Virus

Virus clearance in bronchioalveolar lavage fluids (BAL) and PAMs. ( A ) The viral copy numbers in BAL fluids at necropsy upon challenge with VR2332. ( B ) The viral copy numbers in BAL fluids at necropsy upon challenge with MN184C. Each bar represents the mean value of each group. Bars represent the standard error of the mean. Three independent experiments were performed for each. Significant differences were calculated by Student’s t test (* p < 0.05). ( C ) The viral copy numbers in PAMs at necropsy upon challenge with VR2332. ( D ) The viral copy numbers in PAMs at necropsy upon challenge with MN184C. Each dot represents the mean value of each animal. The bars represent the standard error of the mean. Three independent experiments were performed for each. Student’s t test was used to calculate significant differences (* p < 0.05, ** p < 0.01).

Journal: Vaccines

Article Title: Broad Protection of Pigs against Heterologous PRRSV Strains by a GP5-Mosaic DNA Vaccine Prime/GP5-Mosaic rVaccinia (VACV) Vaccine Boost

doi: 10.3390/vaccines8010106

Figure Lengend Snippet: Virus clearance in bronchioalveolar lavage fluids (BAL) and PAMs. ( A ) The viral copy numbers in BAL fluids at necropsy upon challenge with VR2332. ( B ) The viral copy numbers in BAL fluids at necropsy upon challenge with MN184C. Each bar represents the mean value of each group. Bars represent the standard error of the mean. Three independent experiments were performed for each. Significant differences were calculated by Student’s t test (* p < 0.05). ( C ) The viral copy numbers in PAMs at necropsy upon challenge with VR2332. ( D ) The viral copy numbers in PAMs at necropsy upon challenge with MN184C. Each dot represents the mean value of each animal. The bars represent the standard error of the mean. Three independent experiments were performed for each. Student’s t test was used to calculate significant differences (* p < 0.05, ** p < 0.01).

Article Snippet: The viruses used in the study included the VR2332 NA reference strain (ATCC VR-2332) and the MN184C strain provided kindly by Drs. Kelly Lager and Kay Faaberg at U.S. Department of Agriculture-Agriculture Research Service (USDA-ARS).

Techniques: Virus