Journal: Journal of Applied Clinical Medical Physics

Article Title: Verification of the delivered patient radiation dose for non‐coplanar beam therapy

doi: 10.1002/acm2.13280

Figure Lengend Snippet: Examples of the test treatment plans that use static couch during beam delivery. (a) Non‐coplanar IMRT. (b) Non‐coplanar VMAT.

Article Snippet: Non‐coplanar plans described in more detail below were delivered to a rectangular solid water IMRT QA phantom (CIVCO, Orange City, IA) used in our clinic for SBRT pre‐treatment QA purposes.

Techniques:

Journal: Journal of Applied Clinical Medical Physics

Article Title: Verification of the delivered patient radiation dose for non‐coplanar beam therapy

doi: 10.1002/acm2.13280

Figure Lengend Snippet: Summary of parameters of the static couch test trajectories used.

Article Snippet: Non‐coplanar plans described in more detail below were delivered to a rectangular solid water IMRT QA phantom (CIVCO, Orange City, IA) used in our clinic for SBRT pre‐treatment QA purposes.

Techniques:

Journal: Journal of Bacteriology

Article Title: MutS2 Promotes Homologous Recombination in Bacillus subtilis

doi: 10.1128/JB.00682-16

Figure Lengend Snippet: MutS2 is important for plasmid transformation, and mutS2 deletion is additive with a recU deletion in chromosomal DNA (chrDNA) transformation. (A) Transformation efficiency assay using plasmid DNA. (B) Transformation efficiency using chromosomal DNA. (C) Transformation efficiency assay using chromosomal DNA in the presence of 1 mM IPTG. Transformation efficiency is the ratio of transformants (CFU) to viable cells (CFU). The Mann-Whitney U test was used to determine statistical significance. ns, not significant; *, two-sided P value of <0.05.

Article Snippet: Competent cell cultures were prepared by inoculating a 2-ml culture of LM medium (LB with 3 mM MgSO 4 ) with a single colony and grown at 37°C to an OD 600 between 1 and 1.5 and then transferring 20 μl of the LM culture into 500 μl prewarmed MD medium (1× PC buffer [10× PC buffer is 107 g/liter K 2 HPO 4 , 60 g/liter KH 2 PO 4 , 10 g/liter trisodium citrate · (H 2 O) 5 ], 2% glucose, 50 μg/ml tryptophan, 50 μg/ml phenylalanine, 11 μg/ml ferric ammonium citrate, 2.5 mg/ml potassium aspartate, 3 mM MgSO 4 ) and incubated at 37°C for 4 h. Ten nanograms of pPB153 plasmid DNA (purified from E. coli strain MC1061, which is recA + , resulting in oligomeric plasmid preparations, using the Qiagen plasmid HiSpeed Midi kit with DNA eluted in double-distilled water) or 25 ng of chromosomal DNA (purified using a spin column method; see the supplemental methods) from PEB43 was added to cultures, followed by incubation at 37°C for 1.5 h. The cultures were serially diluted, and 100-μl aliquots of the 10 −6 dilution were plated for viable cells on LB agar.

Techniques: Plasmid Preparation, Transformation Assay, MANN-WHITNEY