recombinant pp1ca Search Results


recombinant pp1ca  (OriGene)
93
OriGene recombinant pp1ca
a) Western blot showing co-immunoprecipitation of CAMK4 with C12ORF57 from Grcc10+/+ whole brain lysate (left) with Grcc10−/− brain negative control (right) showing no GRCC10 expression or CAMK4 pulldown b) Representative CAMK4 and GAPDH western blot bands from brain whole cell lysates of Grcc10 +/+ and Grcc10−/− mice c) Mean relative CAMK4 western blot band intensity normalized to WT (p=.34, N=6 for each genotype Welch’s T-Test) Error bars represent SEM. d) Representative CAMK4, pCAMK4 and lamin (control) bands on western blot from nuclear fraction of Grcc10 WT and KO whole brain lysates e) Relative CAMK4, pCAMK4and Lamin band intensity normalized to Grcc10 WT levels. (p=.001, N=6 for each genotype, 2-way ANOVA with Sidak’s multiple comparison test). f) Representative CAMK4, pCAMK4 and GAPDH (control) bands on western blot from cytoplasmic fractions of whole brain g) Relative CAMK4 pCAMK4 and Lamin band intensity normalized to Grcc10 WT levels (p=.016, N=6 for each genotype, 2-way ANOVA, Sidak’s multiple comparison test) in KO compared to WT. h) Schematic of WT CAMK4 (top) with kinase domain (blue), calmodulin binding domain (purple) and autoinhibitory/regulatory domain (yellow). Construct Δ(322-341) which lacks the autoinhibitory domain (NAI, middle) and construct Δ(305-321) which lacks the calmodulin binding domain (NCB, bottom) i) Representative western blot of co-IP of CAMK4 constructs with C12ORF57-FLAG j ) Relative CAMK4 band intensity between WT, NCB (p=.24, N=8 replicates for each construct, ANOVA) and NAI (p<.0001, N=8 replicates for each construct, ANOVA) CAMK4 constructs.. k ) Relative luminescence from in vitro CAMK4 kinase activity assay for C12ORF57, CAMK4, CAMK4+C12ORF57, <t>CAMK4+PP1CA,</t> CAMK4+PP1CA+C12ORF57, no CAMK4, and no ATP (N=3 per condition, ANOVA). l ) Left: Graph of cumulative frequency (x-axis) of mEPSC against amplitude (pA) in DIV 17 pyramidal neurons including CAMK4 transfected control (green) KO (pink) WT (black), and C12ORF57 transfected (purple). The mean curve of each group is bolded. Right: Mean amplitude of WT, KO, CAMK4 transfected (KO+CAMK4) and C12OR57 transfected (KO+C12ORF57) (p<.0001, N=6 for each group, ANOVA). For all graphs, error bars represent SEM. Statistical differences are indicated in the figures using the following symbols * P ≤ .05, ** P ≤ .01, *** P ≤ .001, **** P < .0001.
Recombinant Pp1ca, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+pp1ca/PPP1A+(PPP1CA)+Mouse+Monoclonal+Antibody/bio_rxiv__2025__01__08__632037-183-4-6
Average 93 stars, based on 1 article reviews
recombinant pp1ca - by Bioz Stars, 2026-11
93/100 stars
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ppp1ca polyclonal antibody  (Elabscience)
N/A
The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 PP1 PP1 is a serine threonine specific protein phosphatase known to be involved in the regulation of a
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ppp1ca antibody (biotin)  (biorbyt)
N/A
Rabbit polyclonal antibody against PPP1CA conjugated to Biotin. Isotype Note: IgG Host Note: Rabbit Conjugation Note: Biotin Reactivity Note: Human Application Note: ELISA
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ppp1ca antibody (oaga02505)  (Aviva Systems)
N/A
Rabbit Polyclonal antibody to PPP1A (protein phosphatase 1, catalytic subunit, alpha isozyme); The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific
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recombinant mouse ppp1ca protein  (Creative BioMart)
N/A
Recombinant Mouse PPP1CA full length or partial length protein was expressed.http://www.creativebiomart.net/Recombinant-Mouse-PPP1CA-Protein-445373.htm
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ppp1ca antibody (oapa00300)  (Aviva Systems)
N/A
PPP1CA; Mouse Anti Human Protein Phosphatase 1 Catalytic Subunit Alpha; Mouse Anti Human Protein Phosphatase 1 Catalytic Subunit Alpha
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ppp1ca antibody  (Abcepta)
N/A
The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 PP1 PP1 is a serine threonine specific protein phosphatase known to be involved in the regulation of a
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anti-ppp1ca monoclonal antibody, clone 2i2-g8-e9  (Creative BioMart)
N/A
This antibody detects endogenous levels PPP1A and does not cross-react with related proteins.Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved
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rabbit anti-human ppp1ca polyclonal antibody  (Cusabio)
N/A
Rabbit anti-Human PPP1CA Polyclonal Antibody
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recombinant zebrafish ppp1cab  (Creative BioMart)
N/A
Recombinant Zebrafish PPP1CAB full length or partial length protein was expressed.http://www.creativebiomart.net/description_432157_12.htm
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ppp1ca antibody (oala02993)  (Aviva Systems)
N/A
Protein Phosphatase 1 alpha (PPP1CA), a PP1 type serine/threonine phosphatase, is a catalytic subunit of Protein Phosphatase 1 which has been shown to function in cell division, glycogen metabolism, muscle contractility and protein synthesis. At
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ppp1a/ppp1ca mouse mab  (Aladdin Scientific Corporation)
N/A
Mouse anti Human PPP1A/PPP1CA Antibody, Monoclonal (1H1-F7-D8), could be used for WB, ICC/IF and so on.Application:WB: 1/500-1/1000ICC/IF: 1/50-1/200Protein FunctionProtein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism,
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Image Search Results


a) Western blot showing co-immunoprecipitation of CAMK4 with C12ORF57 from Grcc10+/+ whole brain lysate (left) with Grcc10−/− brain negative control (right) showing no GRCC10 expression or CAMK4 pulldown b) Representative CAMK4 and GAPDH western blot bands from brain whole cell lysates of Grcc10 +/+ and Grcc10−/− mice c) Mean relative CAMK4 western blot band intensity normalized to WT (p=.34, N=6 for each genotype Welch’s T-Test) Error bars represent SEM. d) Representative CAMK4, pCAMK4 and lamin (control) bands on western blot from nuclear fraction of Grcc10 WT and KO whole brain lysates e) Relative CAMK4, pCAMK4and Lamin band intensity normalized to Grcc10 WT levels. (p=.001, N=6 for each genotype, 2-way ANOVA with Sidak’s multiple comparison test). f) Representative CAMK4, pCAMK4 and GAPDH (control) bands on western blot from cytoplasmic fractions of whole brain g) Relative CAMK4 pCAMK4 and Lamin band intensity normalized to Grcc10 WT levels (p=.016, N=6 for each genotype, 2-way ANOVA, Sidak’s multiple comparison test) in KO compared to WT. h) Schematic of WT CAMK4 (top) with kinase domain (blue), calmodulin binding domain (purple) and autoinhibitory/regulatory domain (yellow). Construct Δ(322-341) which lacks the autoinhibitory domain (NAI, middle) and construct Δ(305-321) which lacks the calmodulin binding domain (NCB, bottom) i) Representative western blot of co-IP of CAMK4 constructs with C12ORF57-FLAG j ) Relative CAMK4 band intensity between WT, NCB (p=.24, N=8 replicates for each construct, ANOVA) and NAI (p<.0001, N=8 replicates for each construct, ANOVA) CAMK4 constructs.. k ) Relative luminescence from in vitro CAMK4 kinase activity assay for C12ORF57, CAMK4, CAMK4+C12ORF57, CAMK4+PP1CA, CAMK4+PP1CA+C12ORF57, no CAMK4, and no ATP (N=3 per condition, ANOVA). l ) Left: Graph of cumulative frequency (x-axis) of mEPSC against amplitude (pA) in DIV 17 pyramidal neurons including CAMK4 transfected control (green) KO (pink) WT (black), and C12ORF57 transfected (purple). The mean curve of each group is bolded. Right: Mean amplitude of WT, KO, CAMK4 transfected (KO+CAMK4) and C12OR57 transfected (KO+C12ORF57) (p<.0001, N=6 for each group, ANOVA). For all graphs, error bars represent SEM. Statistical differences are indicated in the figures using the following symbols * P ≤ .05, ** P ≤ .01, *** P ≤ .001, **** P < .0001.

Journal: bioRxiv

Article Title: C12ORF57: a novel principal regulator of synaptic AMPA currents and excitatory neuronal homeostasis

doi: 10.1101/2025.01.08.632037

Figure Lengend Snippet: a) Western blot showing co-immunoprecipitation of CAMK4 with C12ORF57 from Grcc10+/+ whole brain lysate (left) with Grcc10−/− brain negative control (right) showing no GRCC10 expression or CAMK4 pulldown b) Representative CAMK4 and GAPDH western blot bands from brain whole cell lysates of Grcc10 +/+ and Grcc10−/− mice c) Mean relative CAMK4 western blot band intensity normalized to WT (p=.34, N=6 for each genotype Welch’s T-Test) Error bars represent SEM. d) Representative CAMK4, pCAMK4 and lamin (control) bands on western blot from nuclear fraction of Grcc10 WT and KO whole brain lysates e) Relative CAMK4, pCAMK4and Lamin band intensity normalized to Grcc10 WT levels. (p=.001, N=6 for each genotype, 2-way ANOVA with Sidak’s multiple comparison test). f) Representative CAMK4, pCAMK4 and GAPDH (control) bands on western blot from cytoplasmic fractions of whole brain g) Relative CAMK4 pCAMK4 and Lamin band intensity normalized to Grcc10 WT levels (p=.016, N=6 for each genotype, 2-way ANOVA, Sidak’s multiple comparison test) in KO compared to WT. h) Schematic of WT CAMK4 (top) with kinase domain (blue), calmodulin binding domain (purple) and autoinhibitory/regulatory domain (yellow). Construct Δ(322-341) which lacks the autoinhibitory domain (NAI, middle) and construct Δ(305-321) which lacks the calmodulin binding domain (NCB, bottom) i) Representative western blot of co-IP of CAMK4 constructs with C12ORF57-FLAG j ) Relative CAMK4 band intensity between WT, NCB (p=.24, N=8 replicates for each construct, ANOVA) and NAI (p<.0001, N=8 replicates for each construct, ANOVA) CAMK4 constructs.. k ) Relative luminescence from in vitro CAMK4 kinase activity assay for C12ORF57, CAMK4, CAMK4+C12ORF57, CAMK4+PP1CA, CAMK4+PP1CA+C12ORF57, no CAMK4, and no ATP (N=3 per condition, ANOVA). l ) Left: Graph of cumulative frequency (x-axis) of mEPSC against amplitude (pA) in DIV 17 pyramidal neurons including CAMK4 transfected control (green) KO (pink) WT (black), and C12ORF57 transfected (purple). The mean curve of each group is bolded. Right: Mean amplitude of WT, KO, CAMK4 transfected (KO+CAMK4) and C12OR57 transfected (KO+C12ORF57) (p<.0001, N=6 for each group, ANOVA). For all graphs, error bars represent SEM. Statistical differences are indicated in the figures using the following symbols * P ≤ .05, ** P ≤ .01, *** P ≤ .001, **** P < .0001.

Article Snippet: CAMK4, C12ORF57, and purified recombinant PP1CA (Origene, TA808819) were mixed in equimolar amounts with 25 µM ATP and reaction luminescence was measured on a Tecan Spark for an integration time of 1 sec.

Techniques: Western Blot, Immunoprecipitation, Negative Control, Expressing, Control, Comparison, Binding Assay, Construct, Co-Immunoprecipitation Assay, In Vitro, Kinase Assay, Transfection