recombinant mouse klotho Search Results


recombinant klotho protein  (R&D Systems)
94
R&D Systems recombinant klotho protein
<t>Klotho</t> alleviated DQ-induced cardiomyocyte oxidative stress. (A, B) Cell viability was examined using Cell Counting Kit-8 (CCK-8) assay. (C) Klotho protein levels were examined using western blot analysis. (D) DQ-stimulated H9c2 cardiomyocytes were treated with <t>recombinant</t> Klotho protein, followed by viability assessment using CCK-8 assay. (E, F) Apoptosis was examined using flow cytometry. (G, H) ROS fluorescence signal, (I) MDA level, (J) GSH-ST, (K) GSH-PX, and (L) SOD activities in H9c2 cells were assessed using ELISA kits. (M, N) Nrf2, HO-1, and NQO1 protein levels were examined using western blot analysis. Data were presented as mean ± SD. * P <0.05, ** P <0.01, compared to control group. ## P <0.01, compared to DQ group.
Recombinant Klotho Protein, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+mouse+klotho/Recombinant+Mouse+Klotho+(aa+35-982)+Protein%2C+CF/pmc12709327-49-26-30
Average 94 stars, based on 1 article reviews
recombinant klotho protein - by Bioz Stars, 2026-11
94/100 stars
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materials antibody against mouse βkl  (R&D Systems)
94
R&D Systems materials antibody against mouse βkl
<t>Klotho</t> alleviated DQ-induced cardiomyocyte oxidative stress. (A, B) Cell viability was examined using Cell Counting Kit-8 (CCK-8) assay. (C) Klotho protein levels were examined using western blot analysis. (D) DQ-stimulated H9c2 cardiomyocytes were treated with <t>recombinant</t> Klotho protein, followed by viability assessment using CCK-8 assay. (E, F) Apoptosis was examined using flow cytometry. (G, H) ROS fluorescence signal, (I) MDA level, (J) GSH-ST, (K) GSH-PX, and (L) SOD activities in H9c2 cells were assessed using ELISA kits. (M, N) Nrf2, HO-1, and NQO1 protein levels were examined using western blot analysis. Data were presented as mean ± SD. * P <0.05, ** P <0.01, compared to control group. ## P <0.01, compared to DQ group.
Materials Antibody Against Mouse βkl, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+mouse+klotho/Recombinant+Mouse+Klotho+beta+Protein%2C+CF/pmc04695634-28-0-9
Average 94 stars, based on 1 article reviews
materials antibody against mouse βkl - by Bioz Stars, 2026-11
94/100 stars
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recombinant mouse klotho  (R&D Systems)
94
R&D Systems recombinant mouse klotho
Figure 2. Effect of <t>Klotho</t> on location of FOXO1 in cardiomyocytes upon H/R. FOXO1 protein expression was significantly decreased in the nucleus but increased in the cytoplasm in the H/R group, while in the Klotho treatment groups, FOXO1 protein expression was increased in the nucleus, but reduced in the cytoplasm (red for FOXO1) (magnification, x100). Three indepen dent experiments were performed. FOXO1, forkhead box protein O1; H/R, hypoxia/reoxygenation.
Recombinant Mouse Klotho, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+mouse+klotho/Recombinant+Mouse+Klotho+(aa+35-982)+Protein%2C+CF/pm25377663-48-0-7
Average 94 stars, based on 1 article reviews
recombinant mouse klotho - by Bioz Stars, 2026-11
94/100 stars
  Buy from Supplier
mouse recombinant klotho protein  (Cloud-Clone corp)
90
Cloud-Clone corp mouse recombinant klotho protein
Figure 2. Effect of <t>Klotho</t> on location of FOXO1 in cardiomyocytes upon H/R. FOXO1 protein expression was significantly decreased in the nucleus but increased in the cytoplasm in the H/R group, while in the Klotho treatment groups, FOXO1 protein expression was increased in the nucleus, but reduced in the cytoplasm (red for FOXO1) (magnification, x100). Three indepen dent experiments were performed. FOXO1, forkhead box protein O1; H/R, hypoxia/reoxygenation.
Mouse Recombinant Klotho Protein, supplied by Cloud-Clone corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+mouse+klotho/mouse+recombinant+klotho+protein/ppr0137320-175-3-12
Average 90 stars, based on 1 article reviews
mouse recombinant klotho protein - by Bioz Stars, 2026-11
90/100 stars
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beta klotho protein, mouse, recombinant  (TargetMol)
N/A
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recombinant mouse klotho aa 35 982 protein cf  (R&D Systems)
N/A
The Recombinant Mouse Klotho aa 35 982 Protein from R D Systems is derived from CHO The Recombinant Mouse Klotho aa 35 982 Protein has been validated for the following applications Bioactivity
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recombinant mouse klotho beta protein cf  (R&D Systems)
N/A
The Recombinant Mouse Klotho beta Protein from R D Systems is derived from NS0 The Recombinant Mouse Klotho beta Protein has been validated for the following applications Bioactivity
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recombinant mouse klotho protein, his-tagged  (Creative BioMart)
N/A
Recombinant Mouse Klotho(Ala35-Lys982 (Arg948Lys)) fused with His tag at C-terminal was expressed in CHO.Klotho, also called Klotho-alpha, is the founding member of the Klotho family within the glycosidase-1 superfamily. Klotho is expressed in areas concerned
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Image Search Results


Klotho alleviated DQ-induced cardiomyocyte oxidative stress. (A, B) Cell viability was examined using Cell Counting Kit-8 (CCK-8) assay. (C) Klotho protein levels were examined using western blot analysis. (D) DQ-stimulated H9c2 cardiomyocytes were treated with recombinant Klotho protein, followed by viability assessment using CCK-8 assay. (E, F) Apoptosis was examined using flow cytometry. (G, H) ROS fluorescence signal, (I) MDA level, (J) GSH-ST, (K) GSH-PX, and (L) SOD activities in H9c2 cells were assessed using ELISA kits. (M, N) Nrf2, HO-1, and NQO1 protein levels were examined using western blot analysis. Data were presented as mean ± SD. * P <0.05, ** P <0.01, compared to control group. ## P <0.01, compared to DQ group.

Journal: American Journal of Translational Research

Article Title: Klotho mitigates diquat-induced myocardial injury in rats by activating Nrf2/ARE-mediated suppression of oxidative stress

doi: 10.62347/SKBR3572

Figure Lengend Snippet: Klotho alleviated DQ-induced cardiomyocyte oxidative stress. (A, B) Cell viability was examined using Cell Counting Kit-8 (CCK-8) assay. (C) Klotho protein levels were examined using western blot analysis. (D) DQ-stimulated H9c2 cardiomyocytes were treated with recombinant Klotho protein, followed by viability assessment using CCK-8 assay. (E, F) Apoptosis was examined using flow cytometry. (G, H) ROS fluorescence signal, (I) MDA level, (J) GSH-ST, (K) GSH-PX, and (L) SOD activities in H9c2 cells were assessed using ELISA kits. (M, N) Nrf2, HO-1, and NQO1 protein levels were examined using western blot analysis. Data were presented as mean ± SD. * P <0.05, ** P <0.01, compared to control group. ## P <0.01, compared to DQ group.

Article Snippet: To induce oxidative injury, cells were treated with 50 μM DQ (45422, Sigma-Aldrich, USA) for 24 h. For Klotho intervention, cells were treated with 1 μg/mL recombinant Klotho protein (1819-KL, R&D Systems, USA) [ 19 , 20 ].

Techniques: Cell Counting, CCK-8 Assay, Western Blot, Recombinant, Flow Cytometry, Fluorescence, Enzyme-linked Immunosorbent Assay, Control

Nrf2 inhibition abrogated the protective effects of Klotho on DQ-induced oxidative stress. DQ-stimulated H9c2 cells received recombinant Klotho protein and Nrf2 inhibitor ML385. (A, B) Nrf2, HO-1, and NQO1 protein levels were examined using western blot analysis. (C) Cell viability was examined using CCK-8 assay. (D, E) Apoptosis was examined using flow cytometry. (F, G) ROS fluorescence signal, (H) MDA level, (I) GSH-ST, (J) GSH-PX, and (K) SOD activities in H9c2 cells were assessed using ELISA kits. Data were presented as mean ± SD. ** P <0.01, compared to control group. ## P <0.01, compared to DQ group. && P <0.01, compared to DQ+Klotho group.

Journal: American Journal of Translational Research

Article Title: Klotho mitigates diquat-induced myocardial injury in rats by activating Nrf2/ARE-mediated suppression of oxidative stress

doi: 10.62347/SKBR3572

Figure Lengend Snippet: Nrf2 inhibition abrogated the protective effects of Klotho on DQ-induced oxidative stress. DQ-stimulated H9c2 cells received recombinant Klotho protein and Nrf2 inhibitor ML385. (A, B) Nrf2, HO-1, and NQO1 protein levels were examined using western blot analysis. (C) Cell viability was examined using CCK-8 assay. (D, E) Apoptosis was examined using flow cytometry. (F, G) ROS fluorescence signal, (H) MDA level, (I) GSH-ST, (J) GSH-PX, and (K) SOD activities in H9c2 cells were assessed using ELISA kits. Data were presented as mean ± SD. ** P <0.01, compared to control group. ## P <0.01, compared to DQ group. && P <0.01, compared to DQ+Klotho group.

Article Snippet: To induce oxidative injury, cells were treated with 50 μM DQ (45422, Sigma-Aldrich, USA) for 24 h. For Klotho intervention, cells were treated with 1 μg/mL recombinant Klotho protein (1819-KL, R&D Systems, USA) [ 19 , 20 ].

Techniques: Inhibition, Recombinant, Western Blot, CCK-8 Assay, Flow Cytometry, Fluorescence, Enzyme-linked Immunosorbent Assay, Control

Klotho alleviated DQ-induced acute myocardial injury in rats through Nrf2/ARE activation. Rats were intragastrically administered with DQ to induce acute myocardial injury and treated with recombinant Klotho protein for 5 days. (A, B) Klotho protein expression in myocardial tissues was assessed using immunohistochemistry. (C) H&E staining of myocardial tissue of rats in each group (D, E) ROS fluorescence signal, (F) MDA level, (G) GSH-ST, (H) GSH-PX, and (I) SOD activities in H9c2 cells were assessed using ELISA kits. (J, K) Nrf2, HO-1, and NQO1 protein levels in myocardial tissues was assessed using immunohistochemistry. ** P <0.01, compared to control group. ## P <0.01, compared to DQ group.

Journal: American Journal of Translational Research

Article Title: Klotho mitigates diquat-induced myocardial injury in rats by activating Nrf2/ARE-mediated suppression of oxidative stress

doi: 10.62347/SKBR3572

Figure Lengend Snippet: Klotho alleviated DQ-induced acute myocardial injury in rats through Nrf2/ARE activation. Rats were intragastrically administered with DQ to induce acute myocardial injury and treated with recombinant Klotho protein for 5 days. (A, B) Klotho protein expression in myocardial tissues was assessed using immunohistochemistry. (C) H&E staining of myocardial tissue of rats in each group (D, E) ROS fluorescence signal, (F) MDA level, (G) GSH-ST, (H) GSH-PX, and (I) SOD activities in H9c2 cells were assessed using ELISA kits. (J, K) Nrf2, HO-1, and NQO1 protein levels in myocardial tissues was assessed using immunohistochemistry. ** P <0.01, compared to control group. ## P <0.01, compared to DQ group.

Article Snippet: To induce oxidative injury, cells were treated with 50 μM DQ (45422, Sigma-Aldrich, USA) for 24 h. For Klotho intervention, cells were treated with 1 μg/mL recombinant Klotho protein (1819-KL, R&D Systems, USA) [ 19 , 20 ].

Techniques: Activation Assay, Recombinant, Expressing, Immunohistochemistry, Staining, Fluorescence, Enzyme-linked Immunosorbent Assay, Control

Figure 2. Effect of Klotho on location of FOXO1 in cardiomyocytes upon H/R. FOXO1 protein expression was significantly decreased in the nucleus but increased in the cytoplasm in the H/R group, while in the Klotho treatment groups, FOXO1 protein expression was increased in the nucleus, but reduced in the cytoplasm (red for FOXO1) (magnification, x100). Three indepen dent experiments were performed. FOXO1, forkhead box protein O1; H/R, hypoxia/reoxygenation.

Journal: Molecular medicine reports

Article Title: Protective role of Klotho on cardiomyocytes upon hypoxia/reoxygenation via downregulation of Akt and FOXO1 phosphorylation.

doi: 10.3892/mmr.2014.2899

Figure Lengend Snippet: Figure 2. Effect of Klotho on location of FOXO1 in cardiomyocytes upon H/R. FOXO1 protein expression was significantly decreased in the nucleus but increased in the cytoplasm in the H/R group, while in the Klotho treatment groups, FOXO1 protein expression was increased in the nucleus, but reduced in the cytoplasm (red for FOXO1) (magnification, x100). Three indepen dent experiments were performed. FOXO1, forkhead box protein O1; H/R, hypoxia/reoxygenation.

Article Snippet: Recombinant Mouse Klotho (#1819-KL-050) was purchased from R&D Systems (Minneapolis, MN, USA).

Techniques: Expressing

Figure 3. Effect of Klotho on the location of FOXO1 in cardiomyocytes upon H/R. (A and B) Protein levels of FOXO1 in nucleus and cytoplasm of primary cultured cardiomyocytes. (A) Representative western blot analysis. (B) Quantitative results. *P<0.05 vs. control group; #P<0.05 vs. H/R group. FOXO1, forkhead box protein O1; H/R, hypoxia/reperfusion.

Journal: Molecular medicine reports

Article Title: Protective role of Klotho on cardiomyocytes upon hypoxia/reoxygenation via downregulation of Akt and FOXO1 phosphorylation.

doi: 10.3892/mmr.2014.2899

Figure Lengend Snippet: Figure 3. Effect of Klotho on the location of FOXO1 in cardiomyocytes upon H/R. (A and B) Protein levels of FOXO1 in nucleus and cytoplasm of primary cultured cardiomyocytes. (A) Representative western blot analysis. (B) Quantitative results. *P<0.05 vs. control group; #P<0.05 vs. H/R group. FOXO1, forkhead box protein O1; H/R, hypoxia/reperfusion.

Article Snippet: Recombinant Mouse Klotho (#1819-KL-050) was purchased from R&D Systems (Minneapolis, MN, USA).

Techniques: Cell Culture, Western Blot, Control

Figure 4. Effect of Klotho on FOXO1, p-FOXO1, Akt, p-Akt and SOD2 in cardiomyocytes upon H/R. (A and B) Protein levels of p-FOXO1, t-FOXO1, p-Akt, t-Akt in primary cultured cardiomyocytes. (A) Representative western blots. (B) Quantitative results. *P<0.05 vs. control group; #P<0.05 vs. H/R group. (C and D) Protein levels of SOD2 in primary cultured cardiomyocytes. (C) Representative western blots. (D) Quantitative results. *P<0.05 vs. H/R group. p/t-FOXO1, phospho/total-forkhead box protein O1; SOD2, superoxide dismutase 2; H/R, hypoxia/reperfusion.

Journal: Molecular medicine reports

Article Title: Protective role of Klotho on cardiomyocytes upon hypoxia/reoxygenation via downregulation of Akt and FOXO1 phosphorylation.

doi: 10.3892/mmr.2014.2899

Figure Lengend Snippet: Figure 4. Effect of Klotho on FOXO1, p-FOXO1, Akt, p-Akt and SOD2 in cardiomyocytes upon H/R. (A and B) Protein levels of p-FOXO1, t-FOXO1, p-Akt, t-Akt in primary cultured cardiomyocytes. (A) Representative western blots. (B) Quantitative results. *P<0.05 vs. control group; #P<0.05 vs. H/R group. (C and D) Protein levels of SOD2 in primary cultured cardiomyocytes. (C) Representative western blots. (D) Quantitative results. *P<0.05 vs. H/R group. p/t-FOXO1, phospho/total-forkhead box protein O1; SOD2, superoxide dismutase 2; H/R, hypoxia/reperfusion.

Article Snippet: Recombinant Mouse Klotho (#1819-KL-050) was purchased from R&D Systems (Minneapolis, MN, USA).

Techniques: Cell Culture, Western Blot, Control