recombinant influenza a virus h5n1 Search Results


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    Thermo Fisher h5n1 virus
    Influenza A virus (IAV) infection inhibits expression of DNA methyltransferase 1 (DNMT1), resulting in up-regulation of miR-203. ( A ) A549 cells were infected with <t>H5N1</t> (multiplicity of infection (MOI) = 2) viruses, and abundance of mRNAs encoding three DNA methyltransferases (DNMT1, DNMT3a, and DNMT3b) was measured at different times (0, 2, 4, 6, 12, and 24 h). ( B . ( C . ( D and E ) A549 cells were transfected for 24 h with the pCMV3-DNMT1 plasmid ( D ) or si-DNMT1 ( E ). Next, cells were mock-infected or infected with H5N1 (MOI = 2) viruses for another 24 h to detect abundance of miR-203. The empty vector (pCMV3-empty) and si-control served as negative controls. Data are expressed as the mean + SD of three independent experiments. * p
    H5n1 Virus, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/h5n1 virus/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    h5n1 virus - by Bioz Stars, 2021-09
    93/100 stars
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    86
    Sino Biological h5n1
    Influenza A virus (IAV) infection inhibits expression of DNA methyltransferase 1 (DNMT1), resulting in up-regulation of miR-203. ( A ) A549 cells were infected with <t>H5N1</t> (multiplicity of infection (MOI) = 2) viruses, and abundance of mRNAs encoding three DNA methyltransferases (DNMT1, DNMT3a, and DNMT3b) was measured at different times (0, 2, 4, 6, 12, and 24 h). ( B . ( C . ( D and E ) A549 cells were transfected for 24 h with the pCMV3-DNMT1 plasmid ( D ) or si-DNMT1 ( E ). Next, cells were mock-infected or infected with H5N1 (MOI = 2) viruses for another 24 h to detect abundance of miR-203. The empty vector (pCMV3-empty) and si-control served as negative controls. Data are expressed as the mean + SD of three independent experiments. * p
    H5n1, supplied by Sino Biological, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/h5n1/product/Sino Biological
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    h5n1 - by Bioz Stars, 2021-09
    86/100 stars
      Buy from Supplier

    94
    Sino Biological influenza a h5n1 hemagglutinin ha protein
    Influenza A virus (IAV) infection inhibits expression of DNA methyltransferase 1 (DNMT1), resulting in up-regulation of miR-203. ( A ) A549 cells were infected with <t>H5N1</t> (multiplicity of infection (MOI) = 2) viruses, and abundance of mRNAs encoding three DNA methyltransferases (DNMT1, DNMT3a, and DNMT3b) was measured at different times (0, 2, 4, 6, 12, and 24 h). ( B . ( C . ( D and E ) A549 cells were transfected for 24 h with the pCMV3-DNMT1 plasmid ( D ) or si-DNMT1 ( E ). Next, cells were mock-infected or infected with H5N1 (MOI = 2) viruses for another 24 h to detect abundance of miR-203. The empty vector (pCMV3-empty) and si-control served as negative controls. Data are expressed as the mean + SD of three independent experiments. * p
    Influenza A H5n1 Hemagglutinin Ha Protein, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/influenza a h5n1 hemagglutinin ha protein/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    influenza a h5n1 hemagglutinin ha protein - by Bioz Stars, 2021-09
    94/100 stars
      Buy from Supplier

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    Influenza A virus (IAV) infection inhibits expression of DNA methyltransferase 1 (DNMT1), resulting in up-regulation of miR-203. ( A ) A549 cells were infected with H5N1 (multiplicity of infection (MOI) = 2) viruses, and abundance of mRNAs encoding three DNA methyltransferases (DNMT1, DNMT3a, and DNMT3b) was measured at different times (0, 2, 4, 6, 12, and 24 h). ( B . ( C . ( D and E ) A549 cells were transfected for 24 h with the pCMV3-DNMT1 plasmid ( D ) or si-DNMT1 ( E ). Next, cells were mock-infected or infected with H5N1 (MOI = 2) viruses for another 24 h to detect abundance of miR-203. The empty vector (pCMV3-empty) and si-control served as negative controls. Data are expressed as the mean + SD of three independent experiments. * p

    Journal: Scientific Reports

    Article Title: Up-regulation of microRNA-203 in influenza A virus infection inhibits viral replication by targeting DR1

    doi: 10.1038/s41598-018-25073-9

    Figure Lengend Snippet: Influenza A virus (IAV) infection inhibits expression of DNA methyltransferase 1 (DNMT1), resulting in up-regulation of miR-203. ( A ) A549 cells were infected with H5N1 (multiplicity of infection (MOI) = 2) viruses, and abundance of mRNAs encoding three DNA methyltransferases (DNMT1, DNMT3a, and DNMT3b) was measured at different times (0, 2, 4, 6, 12, and 24 h). ( B . ( C . ( D and E ) A549 cells were transfected for 24 h with the pCMV3-DNMT1 plasmid ( D ) or si-DNMT1 ( E ). Next, cells were mock-infected or infected with H5N1 (MOI = 2) viruses for another 24 h to detect abundance of miR-203. The empty vector (pCMV3-empty) and si-control served as negative controls. Data are expressed as the mean + SD of three independent experiments. * p

    Article Snippet: For miRNA expression profiling analysis, A549 cells were infected with H1N1 (MOI = 5) or H5N1 (MOI = 2) viruses for 24 or 48 h. Total RNA was extracted with TRIzol® Reagent (Thermo Fisher Scientific) and miRNA expression profiling analysis was conducted by Gene Square Bio-technology Ltd (Beijing, China P.R.) using a Human v2 MicroRNA Expression Profiling Kit (Illumina, San Diego, CA, USA).

    Techniques: Infection, Expressing, Transfection, Plasmid Preparation

    Type I interferon (IFN) participates in transcriptional regulation of miR-203 to promote its expression directly. ( A ) A549 cells were infected with H5N1 (MOI = 2) viruses and harvested at different times (2, 6, 12, and 24 h). The abundance of IFN-α and IFN-β mRNA was measured by quantitative real-time PCR (qPCR). ( B and C ) A549 cells ( B ) and Vero cells ( C ) were treated with IFN-α (2000 units/ml) for 12 h, and expression of miR-203 was measured by qPCR. (D) A549 cells were co-transfected for 24 h with luciferase reporter vectors (pGL3-promoter) and a pRL-TK plasmid (pGL3-basic vectors were used as a negative control). Then, cells were treated with IFN-α for another 12 h followed by measurement of luciferase activity in a dual-luciferase assay. The results are presented as the normalized ratio of Firefly to Renilla luciferase activity. ( E ) Vero cells were mock-infected or infected with H5N1 (MOI = 2) viruses. Cells were harvested at different times (12, 24, 36, and 48 h), and abundance of miR-203 was measured by qPCR. Data are expressed as the mean + SD of three independent experiments. * p

    Journal: Scientific Reports

    Article Title: Up-regulation of microRNA-203 in influenza A virus infection inhibits viral replication by targeting DR1

    doi: 10.1038/s41598-018-25073-9

    Figure Lengend Snippet: Type I interferon (IFN) participates in transcriptional regulation of miR-203 to promote its expression directly. ( A ) A549 cells were infected with H5N1 (MOI = 2) viruses and harvested at different times (2, 6, 12, and 24 h). The abundance of IFN-α and IFN-β mRNA was measured by quantitative real-time PCR (qPCR). ( B and C ) A549 cells ( B ) and Vero cells ( C ) were treated with IFN-α (2000 units/ml) for 12 h, and expression of miR-203 was measured by qPCR. (D) A549 cells were co-transfected for 24 h with luciferase reporter vectors (pGL3-promoter) and a pRL-TK plasmid (pGL3-basic vectors were used as a negative control). Then, cells were treated with IFN-α for another 12 h followed by measurement of luciferase activity in a dual-luciferase assay. The results are presented as the normalized ratio of Firefly to Renilla luciferase activity. ( E ) Vero cells were mock-infected or infected with H5N1 (MOI = 2) viruses. Cells were harvested at different times (12, 24, 36, and 48 h), and abundance of miR-203 was measured by qPCR. Data are expressed as the mean + SD of three independent experiments. * p

    Article Snippet: For miRNA expression profiling analysis, A549 cells were infected with H1N1 (MOI = 5) or H5N1 (MOI = 2) viruses for 24 or 48 h. Total RNA was extracted with TRIzol® Reagent (Thermo Fisher Scientific) and miRNA expression profiling analysis was conducted by Gene Square Bio-technology Ltd (Beijing, China P.R.) using a Human v2 MicroRNA Expression Profiling Kit (Illumina, San Diego, CA, USA).

    Techniques: Expressing, Infection, Real-time Polymerase Chain Reaction, Transfection, Luciferase, Plasmid Preparation, Negative Control, Activity Assay

    IAV-mediated demethylation of the miR-203 promoter region up-regulates expression of miR-203. ( A ) Bisulfite sequencing PCR of the miR-203 promoter region was performed in A549 cells mock-infected or infected with H5N1 (MOI = 2) viruses for 24 h. The sequenced region covered −600 to −10 bp within the miR-203 promoter region. Three lines denote three replicates in per group. Open circles, unmethylated; solid circles, methylated. ( B ) A549 cells were treated for 48 h with 5-aza-2′-deoxycytidine, a methylation inhibitor, at a final concentration of 0.5, 1, or 2 μM, and total RNA was purified to analyze abundance of miR-203 by quantitative real-time PCR (qPCR). ( C ) Vero cells were treated for 48 h with 5-aza-2′-deoxycytidine (final concentration, 1 μM) and total RNA purified. The abundance of miR-203 was measured by qPCR. Data are expressed as the mean + SD of three independent experiments. * p

    Journal: Scientific Reports

    Article Title: Up-regulation of microRNA-203 in influenza A virus infection inhibits viral replication by targeting DR1

    doi: 10.1038/s41598-018-25073-9

    Figure Lengend Snippet: IAV-mediated demethylation of the miR-203 promoter region up-regulates expression of miR-203. ( A ) Bisulfite sequencing PCR of the miR-203 promoter region was performed in A549 cells mock-infected or infected with H5N1 (MOI = 2) viruses for 24 h. The sequenced region covered −600 to −10 bp within the miR-203 promoter region. Three lines denote three replicates in per group. Open circles, unmethylated; solid circles, methylated. ( B ) A549 cells were treated for 48 h with 5-aza-2′-deoxycytidine, a methylation inhibitor, at a final concentration of 0.5, 1, or 2 μM, and total RNA was purified to analyze abundance of miR-203 by quantitative real-time PCR (qPCR). ( C ) Vero cells were treated for 48 h with 5-aza-2′-deoxycytidine (final concentration, 1 μM) and total RNA purified. The abundance of miR-203 was measured by qPCR. Data are expressed as the mean + SD of three independent experiments. * p

    Article Snippet: For miRNA expression profiling analysis, A549 cells were infected with H1N1 (MOI = 5) or H5N1 (MOI = 2) viruses for 24 or 48 h. Total RNA was extracted with TRIzol® Reagent (Thermo Fisher Scientific) and miRNA expression profiling analysis was conducted by Gene Square Bio-technology Ltd (Beijing, China P.R.) using a Human v2 MicroRNA Expression Profiling Kit (Illumina, San Diego, CA, USA).

    Techniques: Expressing, Methylation Sequencing, Polymerase Chain Reaction, Infection, Methylation, Concentration Assay, Purification, Real-time Polymerase Chain Reaction

    Influenza A virus (IAV) infection of A549 cells up-regulates expression of miR-203. ( A ) Venn diagram illustrates the numbers of miRNAs identified in each group and the number of common miRNAs within the four groups. ( B ) Heatmap of miRNA expression in A549 cells infected with H1N1 (501) and H5N1 viruses for 24 or 48 h. Each value for the virus/mock average signal ratio was expressed as log10 when drawing the Heatmap. Red denotes up-regulation, while green denotes down-regulation. ( C ) A549 cells were mock-infected or infected with H1N1 (501), H1N1 (PR8), H3N2, H5N1, or H7N9 for 24 or 48 h, and abundance of miR-203 was assessed by quantitative real-time PCR (qPCR). ( D and E ) A549 cells were mock-infected or infected with H1N1 (501) ( D ) or H5N1 viruses ( E ). Cells were harvested at different times (12, 24, 36, and 48 h), and dynamic changes in miR-203 expression were assessed by qPCR. Data are expressed as the mean ± SD of three independent experiments. * p

    Journal: Scientific Reports

    Article Title: Up-regulation of microRNA-203 in influenza A virus infection inhibits viral replication by targeting DR1

    doi: 10.1038/s41598-018-25073-9

    Figure Lengend Snippet: Influenza A virus (IAV) infection of A549 cells up-regulates expression of miR-203. ( A ) Venn diagram illustrates the numbers of miRNAs identified in each group and the number of common miRNAs within the four groups. ( B ) Heatmap of miRNA expression in A549 cells infected with H1N1 (501) and H5N1 viruses for 24 or 48 h. Each value for the virus/mock average signal ratio was expressed as log10 when drawing the Heatmap. Red denotes up-regulation, while green denotes down-regulation. ( C ) A549 cells were mock-infected or infected with H1N1 (501), H1N1 (PR8), H3N2, H5N1, or H7N9 for 24 or 48 h, and abundance of miR-203 was assessed by quantitative real-time PCR (qPCR). ( D and E ) A549 cells were mock-infected or infected with H1N1 (501) ( D ) or H5N1 viruses ( E ). Cells were harvested at different times (12, 24, 36, and 48 h), and dynamic changes in miR-203 expression were assessed by qPCR. Data are expressed as the mean ± SD of three independent experiments. * p

    Article Snippet: For miRNA expression profiling analysis, A549 cells were infected with H1N1 (MOI = 5) or H5N1 (MOI = 2) viruses for 24 or 48 h. Total RNA was extracted with TRIzol® Reagent (Thermo Fisher Scientific) and miRNA expression profiling analysis was conducted by Gene Square Bio-technology Ltd (Beijing, China P.R.) using a Human v2 MicroRNA Expression Profiling Kit (Illumina, San Diego, CA, USA).

    Techniques: Infection, Expressing, Real-time Polymerase Chain Reaction