Journal: Cell Communication and Signaling : CCS

Article Title: Mitochondria-mediated ferroptosis induced by CARD9 ablation prevents MDSCs-dependent antifungal immunity

doi: 10.1186/s12964-024-01581-2

Figure Lengend Snippet: FosB controls Slc7a11 transcription in MDSCs during C. tropicalis stimulation. (A) Heat map demonstrating differentially expressed genes in the kidneys from Card9 −/− mice infected with C. tropicalis compared with WT mice. (B) The expression levels of Fosb gene in MDSCs treated with or without C. tropicalis for 6 h from RNA-sequencing analysis. (C) WT and Card9 −/− MDSCs were stimulated with C. tropicalis (MOI = 1) for 6 h. The expression of Fosb mRNA was detected by qPCR. (D) WT and Card9 −/− MDSCs were stimulated with C. tropicalis (MOI = 1) for 24 h. The protein level of FosB was measured by western blot. (E-G) WT MDSCs were transfected with Fosb specific siRNA for 24 h, then the cells were stimulated with or without C. tropicalis (MOI = 1) for 6 h (E and F) or 24 h (G) . The expression of FosB and SLC7A11 were detected by qPCR and western blot assays. (H) The logo of FosB-binding site from the JASPAR CORE database ( https://jaspar.genereg.net/ ). (I) One potential FosB-binding site in the Slc7a11 promoter were predicted by using the JASPAR CORE database. (J) WT and Card9 −/− MDSCs were stimulated with or without C. tropicalis (MOI = 1) for 6 h. ChIP-qPCR analysis of the interaction between FosB and Slc7a11 promoter binding site. Data with error bars are expressed as mean ± SEM, (A , n = 6 ) (B-G and J , n = 3 ) . Each panel shows at least six or three independent biological replicates from a typical experiment. Statistical analysis was determined by the unpaired Student’s t -test and two-way ANOVA with Bonferroni’s multiple comparisons test for post-hoc test. ns (not significant), P > 0.05; * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: In brief, ChIP was implemented by utilizing the antibodies against FosB (CST, 2251S, 1:50) and Rabbit IgG control (CST, 2729, 1:50), and 30 μL ChIP-Grade Protein G Agarose Beads. qPCR analysis was conducted to determine the ChIP-enriched DNA fragments.

Techniques: Infection, Expressing, RNA Sequencing, Western Blot, Transfection, Binding Assay, ChIP-qPCR

Journal: BMC Medical Informatics and Decision Making

Article Title: Implementation challenges and perception of care providers on Electronic Medical Records at St. Paul’s and Ayder Hospitals, Ethiopia

doi: 10.1186/s12911-021-01670-z

Figure Lengend Snippet: Respondents perception and willingness to use EMR for future clinical practice

Article Snippet: The respondent further explored that “later on, the establishment of an ICT infrastructure across the hospital and the university with IT facilities, equipment’s and well-equipped data center, fortunately, bucked the SmartCare EMR implementation and contributed to have a sustainable system to the current date at Ayder” (Sys Admin 003).

Techniques:

Journal: BMC Medical Informatics and Decision Making

Article Title: Implementation challenges and perception of care providers on Electronic Medical Records at St. Paul’s and Ayder Hospitals, Ethiopia

doi: 10.1186/s12911-021-01670-z

Figure Lengend Snippet: Summary of qualitative data general themes, sub-themes and response categories

Article Snippet: The respondent further explored that “later on, the establishment of an ICT infrastructure across the hospital and the university with IT facilities, equipment’s and well-equipped data center, fortunately, bucked the SmartCare EMR implementation and contributed to have a sustainable system to the current date at Ayder” (Sys Admin 003).

Techniques: Software