Journal: Cell Reports

Article Title: Stromal Cell-Contact Dependent PI3K and APRIL Induced NF-κB Signaling Prevent Mitochondrial- and ER Stress Induced Death of Memory Plasma Cells

doi: 10.1016/j.celrep.2020.107982

Figure Lengend Snippet:

Article Snippet: Anti-mouse BCL2, APC, REA356 , Miltenyi Biotec , Catalog # 130-105-474; RRID: AB_2651266.

Techniques: Recombinant, Electron Microscopy, Staining, Software

Journal: Cell reports

Article Title: Integration of T helper and BCR signals governs enhanced plasma cell differentiation of memory B cells by regulation of CD45 phosphatase activity

doi: 10.1016/j.celrep.2021.109525

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: APC-Vio770 Recombinant human anti B220(clone REA755) , Miltenyi Biotec , Cat# 130–110-849; RRID:AB_2658286.

Techniques: Negative Control, Recombinant, Purification, Staining, Gene Expression, Lysis, Immunoprecipitation, Plasmid Preparation, Enzyme-linked Immunosorbent Assay, Blocking Assay, Cell Isolation, Software, Microscopy

Journal: Clinical & Translational Immunology

Article Title: Assessment of enhanced influenza vaccination finds that FluAd conveys an advantage in mice and older adults

doi: 10.1002/cti2.1107

Figure Lengend Snippet: Elevated CD4 + Tfh and GC B cells by increased expression of SHM and CSR genes lead to early IgG class switch for A‐eIIV. Vaccine responses were assessed by FACS (a) for CD4 + Tfh cells (b) and GC B cells (c) at days 7 and 21 post‐vaccination in the iLN. Expression of AID, Bcl6, IRF4, Pax5 and c‐Myc in B cells from iLN (relative expression to GAPDH expression, expressed in Δ C t ) at day 7 post‐vaccination (d) . Quantity (f) and proportion (g) of H3N2‐1968 HA‐specific IgM and IgG at days 7 and 21 post‐vaccination, measured by ELISA AUC endpoint titres (Supplementary figure ). Data represent the mean, SEM and individual responses, n = 4 or 5 mice per group. *shows statistical significance by one‐way ANOVA (mixed model) for eIIV versus S‐IIV, * P < 0.05, ** P < 0.01 and *** P < 0.005, experiments were repeated twice. Representative FACS plots were gated on lymphocytes for FSC/SSC and live/dead (a) .

Article Snippet: Quantitative RT‐PCR was performed for the murine Aicda (AID), Bcl6, Pax5, IRF4 and C‐Myc genes on B cells isolated from mouse iLNs using magnetic B‐cell isolation kit (Miltenyi Biotec, Bergisch Gladbach, Germany).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay

Journal: Clinical & Translational Immunology

Article Title: Assessment of enhanced influenza vaccination finds that FluAd conveys an advantage in mice and older adults

doi: 10.1002/cti2.1107

Figure Lengend Snippet: Influenza‐specific T‐ and B‐cell recall responses increased by A‐eIIV after H3N2‐1968 challenge. Vaccinated mice were challenged i.n. with 20LD 50 of H3N2‐1968 virus at 6 months (long‐term memory) post‐vaccination, and at day 7 post‐infection, lungs and BALF were collected. Long‐term memory H3N2‐1968 challenge weight loss (a) , lung viral load (b) and protein concentration in BALF (c) . By FACS, polyfunctional H3N2‐1968‐specific CD8 + T cells (IFN‐γ + TNF‐α + IL‐2 + ) were measured in the BAL (d) . By FACS (e) , fluorescent‐labelled recombinant HA probes for H3‐2016‐specific B cells were measured in the mLN (f) . Expression of AID, Bcl6, IRF4, Pax5 and c‐Myc in B cells from spleen (relative expression to GAPDH expression, expressed in 2 - Δ C t ) (g) . Data represent the mean, SEM and individual responses of n = 4 mice per group. In e , the percentage of the population of interest is shown in black on the FACS plots, and the ratio of the mean percentage versus PBS is shown in red. ^shows statistical significance of S‐IIV versus PBS. *shows statistical significance of eIIV versus S‐IIV. * P < 0.05, ** P < 0.01 and *** P < 0.005, experiments were repeated twice.

Article Snippet: Quantitative RT‐PCR was performed for the murine Aicda (AID), Bcl6, Pax5, IRF4 and C‐Myc genes on B cells isolated from mouse iLNs using magnetic B‐cell isolation kit (Miltenyi Biotec, Bergisch Gladbach, Germany).

Techniques: Virus, Infection, Protein Concentration, Recombinant, Expressing