Journal: Frontiers in Veterinary Science

Article Title: Pharmacokinetics and Tissue Levels of Pantoprazole in Neonatal Calves After Intravenous Administration

doi: 10.3389/fvets.2020.580735

Figure Lengend Snippet: Mean plasma pantoprazole concentration (logarithmic scale) vs. time (hr) profiles for neonatal calves ( n = 9) following intravenous (IV) single dose administration of 1.0 mg/kg of pantoprazole.

Article Snippet: Pantoprazole sulfone (Toronto Research Chemicals, Ontario, Canada) became the analyte spiked into blank tissue samples to generate calibration spikes and QC samples.

Techniques: Clinical Proteomics, Concentration Assay

Journal: Frontiers in Veterinary Science

Article Title: Pharmacokinetics and Tissue Levels of Pantoprazole in Neonatal Calves After Intravenous Administration

doi: 10.3389/fvets.2020.580735

Figure Lengend Snippet: Pantoprazole pharmacokinetic parameters following a single intravenous (1 mg/kg) administration to neonatal Holstein calves.

Article Snippet: Pantoprazole sulfone (Toronto Research Chemicals, Ontario, Canada) became the analyte spiked into blank tissue samples to generate calibration spikes and QC samples.

Techniques:

Journal: Frontiers in Veterinary Science

Article Title: Pharmacokinetics and Tissue Levels of Pantoprazole in Neonatal Calves After Intravenous Administration

doi: 10.3389/fvets.2020.580735

Figure Lengend Snippet: Tissue concentrations of pantoprazole sulfone (μg/g) in collected tissues 1, 3, and 5 days after intravenous administration of pantoprazole (1 mg/kg) from study calves.

Article Snippet: Pantoprazole sulfone (Toronto Research Chemicals, Ontario, Canada) became the analyte spiked into blank tissue samples to generate calibration spikes and QC samples.

Techniques:

Journal: Frontiers in Veterinary Science

Article Title: Pharmacokinetics and Tissue Levels of Pantoprazole in Neonatal Calves After Intravenous Administration

doi: 10.3389/fvets.2020.580735

Figure Lengend Snippet: Comparisons of pharmacokinetic parameters of pantoprazole in domestic animal species, after single dose intravenous administration.

Article Snippet: Pantoprazole sulfone (Toronto Research Chemicals, Ontario, Canada) became the analyte spiked into blank tissue samples to generate calibration spikes and QC samples.

Techniques:

Journal: Endocrine Related Cancer

Article Title: Array-CGH identifies cyclin D1 and UBCH10 amplicons in anaplastic thyroid carcinoma

doi: 10.1677/erc-08-0018

Figure Lengend Snippet: Figure 2 (A) Array-CGH profiles of chromosome 11 for case 7 (upper) carrying amplification in 11q22.1 and gain in 11q13, and for case 4 (below) harboring an 11q13 amplicon. (B) Western blot analyses showing cyclin D1 expression in positive control cells (C), and ATCs 21, 25, and 27, while normal thyroid (N) and ATCs 22, 23, and 24 are negative. Incubation of the same filter with a-actinin served as loading control. (C) Fluorescence in situ hybridization (FISH) of CCND1 (Cyclin D1, red) and centromere 11 (CEP11, green) copy numbers. Two signals are observed in normal metaphase and interphase nuclei, while ATC cases 4 and 7, and HTh 7 cells show relative gain of CCND1.

Article Snippet: The CCND1 plasmid was obtained from Addgene (Rc/CMV-CCND1 #8962, Cambridge, MA, USA) and the control plasmid Rc/CMV was kindly provided by Dr Sue Firth at the Kolling Institute of Medical Research, NSW, Australia.

Techniques: Amplification, Western Blot, Expressing, Positive Control, Incubation, Control, Fluorescence, In Situ Hybridization

Journal: Endocrine Related Cancer

Article Title: Array-CGH identifies cyclin D1 and UBCH10 amplicons in anaplastic thyroid carcinoma

doi: 10.1677/erc-08-0018

Figure Lengend Snippet: Figure 5 Analysis of CCND1 (cyclin D1) effect on thyroid cell growth. Comparison plot for proliferation (MTS) assay (average of three independent experiments) of (A) HTh 7 ATC cells transfected against CCND1 siRNA and its reference control cells at 0, 24, 48, and 72 h after transfection, and (B) Nthy-ori 3-1 normal thyroid cells transfected with CCND1 or empty vector (pRC/CMV). Standard errors are indicated at each time point. Efficiency of siRNA as well as CCND1 transfection was validated by western blot analysis and qRT-PCR. Quantification was performed against a-tubulin for the western blot analysis and against 18S for qRT-PCR.

Article Snippet: The CCND1 plasmid was obtained from Addgene (Rc/CMV-CCND1 #8962, Cambridge, MA, USA) and the control plasmid Rc/CMV was kindly provided by Dr Sue Firth at the Kolling Institute of Medical Research, NSW, Australia.

Techniques: Comparison, MTS Assay, Transfection, Control, Plasmid Preparation, Western Blot, Quantitative RT-PCR