Journal: Scientific Reports

Article Title: Multi-omics analysis reveals that ginsenoside Rb1 improves prognostic outcomes in sepsis by modulating mitochondrial metabolism MTHFD2 targets

doi: 10.1038/s41598-026-37362-9

Figure Lengend Snippet: Grb1 significantly reduces inflammatory levels and improves survival outcome in CLP rats. ( A ) Identification of MTHFD2 drug targets using molecular docking techniques. ( B ) mRNA expression analysis of MTHFD2 in control, CLP, and CLP+Grb1 groups (n = 6). ( C , D ) Survival analysis of control, CLP, and CLP+Grb1 groups (n = 6). ( E – G ) Quantitative analysis of IL-1β, IL-6, and TNF-α levels in control, CLP, and CLP+Grb1 groups (n = 6). ( H ) Wet/dry ratio analysis of the left lung (n = 6). ( I , J ) Quantitative analysis of creatinine and ALT levels in CLP and CLP+Grb1 groups (n = 6). Data are presented as mean ± SD; statistical significance: * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Rats were randomly assigned to three groups: Sham, CLP, and Ginsenoside Rb1 (Grb1)-treated CLP (CLP+Grb1) group (HY-N0039, MedChemExpress).

Techniques: Expressing, Control

Journal: Journal of Nanobiotechnology

Article Title: Carbon quantum dots of ginsenoside Rb1 for application in a mouse model of intracerebral Hemorrhage

doi: 10.1186/s12951-024-02368-w

Figure Lengend Snippet: Effective clearance of oxygen free radicals and iron ions by RBCQDs. ( a ) FT-IR spectra of ethylenediamine (blue), ginsenoside Rb1 (black), and RBCQDs (red). XPS spectra of RBCQDs, including C1s ( b ), O1s ( c ), and N1s ( d ) exemplary spectra. The clearance rates of ABTS ( e ) and DPPH ( f ) radicals by RBCQDs and ginsenoside Rb1 at various concentrations ( n = 6/group, two-way ANOVA). ( g ) Appearance before and after RBCQDs, Fe 3+ , and RBCQDs + Fe 3+ centrifugation. ( h ) Appearance before and after centrifugation of RBCQDs, Fe 2+ , and RBCQDs + Fe 2+ . ( i ) Fluorescence intensity of RBCQDs after incubation with 0, 0.05, 0.15, 0.25, 0.5, and 1 mM Fe 2+ . ( j ) Fluorescence intensity of RBCQDs after incubation with 0, 0.05, 0.15, 0.25, 0.5, and 1 mM Fe 3+ . ( k ) UV absorption spectra of RBCQDs at different concentrations after incubation with Fe 2+ for 12 h. ( l ) UV absorption spectra of RBCQDs at different concentrations after incubation with Fe 3+ for 12 h

Article Snippet: Fourier transform infrared spectrometry (Nicolet iS50, Thermo Scientific) was used to investigate the infrared absorption spectra of ginsenoside Rb1, anhydrous ethylenediamine, and RBCQDs, with samples prepared using a potassium bromide pellet method.

Techniques: Centrifugation, Fluorescence, Incubation

Journal: Cancer Science

Article Title: Ubiquitin C‐terminal hydrolase‐L1 has prognostic relevance and is a therapeutic target for high‐grade neuroendocrine lung cancers

doi: 10.1111/cas.14284

Figure Lengend Snippet: Protein expression of UCHL1, p53 and RB1, and mRNA expression of UCHL1 in various lung cancer cell lines and normal lung epithelial cells. A, Increased UCHL1 levels in all small cell lung cancers (SCLC) tested (H82, H69 and H526) and in three non–small cell lung cancer (NSCLC) cell lines (A549, H1299 and PC9) was confirmed by western blotting. B, Comparison of UCHL1/β‐actin protein expression ratio among the cell lines showed that SCLC consistently had high UCHL1 levels, whereas NSCLC showed different expression patterns depending on the cell line. C, Comparison of UCHL1 mRNA expression among the cell lines showed that all lung cancer cell lines except for HCC827 had high UCHL1 levels

Article Snippet: The following were used as primary antibodies: UCHL1 (#13179; CST), p53 (#9282; CST), RB1 (MAB6495; R&D Systems), β‐actin (A5316; Sigma‐Aldrich), CD9 (EXOAB‐CD9A‐1; SBI) and CD63 (EXOAB‐CD63A‐1; SBI).

Techniques: Expressing, Western Blot, Comparison