Journal: NPJ Biofilms and Microbiomes

Article Title: In silico identification of two peptides with antibacterial activity against multidrug-resistant Staphylococcus aureus

doi: 10.1038/s41522-022-00320-0

Figure Lengend Snippet: Inhibitory action of HG2 and HG4 on LPS or LTA-driven inflammation in murine macrophages: eLUCidate™ Raw 264.7 NF-kB reporter cell line was used to measure the inhibitory action of HG2 and HG4 on LPS ( a ) or LTA ( b ) -mediated inflammation as explained in the Materials and Methods’ section. Data were plotted using GraphPad® Prism 7 software. Results are expressed as means ± standard deviation. Representative images of toxicity assay of peptides ( c ) — ( i ) HG2 and ( ii ) HG4 in G. mellonella 120 h post-treatment with 3x MIC concentrations. The larvae remained alive and without melanisation. Virulence assay of MRSA USA300 in G. mellonella using a lethal dose inoculum of 10 6 CFU/per larvae—( iii ) 24 h post-infection: some larvae were dead and partial melanisation was observed. ( iv ). 48 h post-infection: most larvae were dead and complete melanisation was evident. The experiment was done with three experimental replicates, each containing groups of 10 larvae. d Kaplan–Meier survival curves of G. mellonella infected with a lethal dose (LD 50 ) of S. aureus (2.25 × 10 6 CFU/larvae) and treated with peptides HG2 and HG4 at 1x and 3x MIC concentrations and uninfected larvae treated with peptides in PBS at 3x MIC (placebo showing a 100% larvae survival rate).

Article Snippet: The inhibitory activity of HG2, HG4 and comparator compounds against LPS and LTA was assessed using eLUCidate™ Raw 264.7 NF-kB reporter cell line (AMSBIO) as previously described .

Techniques: Software, Standard Deviation, Infection

Journal: Microorganisms

Article Title: A Plasmid-Encoded FetMP-Fls Iron Uptake System Confers Selective Advantages to Salmonella enterica Serovar Typhimurium in Growth under Iron-Restricted Conditions and for Infection of Mammalian Host Cells

doi: 10.3390/microorganisms8050630

Figure Lengend Snippet: Invasion of epithelial HeLa cells ( A ) and replication within RAW264.7 macrophages ( B ) by wild-type S. Typhimurium LSP 146/02, its Δ fetMP - flsDA isogenic derivative, and the latter strain complemented with the cloned fetMP - flsDA genes (LSP 146/02 Δ fetMP - flsDA C ). The data shown are the mean values ± standard deviations of three biological replicates. Statistical significance (**** p < 0.0001; *** p < 0.001; ** p < 0.01; * p < 0.05; n.s., not significant) was determined by one-way ANOVA and the Tukey´s post-test.

Article Snippet: HeLa and RAW264.7 cells (Cell Lines Service (CLS), Heidelberg, Germany) were propagated in a high-glucose (4.5 g/L) Dulbecco’s modified Eagle’s medium (DMEM) containing 4 mM glutamine (PAA, Cölbe, Germany) and 10% (HeLa) or 6% (RAW264.7) inactivated fetal calf serum (iFCS) (Sigma), at 37 °C in an atmosphere of 5% CO 2 .

Techniques: Clone Assay

Journal: Microorganisms

Article Title: A Plasmid-Encoded FetMP-Fls Iron Uptake System Confers Selective Advantages to Salmonella enterica Serovar Typhimurium in Growth under Iron-Restricted Conditions and for Infection of Mammalian Host Cells

doi: 10.3390/microorganisms8050630

Figure Lengend Snippet: Invasion of epithelial HeLa cells by S. Typhimurium ATCC 14028 harboring the cloned fetMP - flsDA genes (ATCC 14028 fetMP - flsDA T ) ( A ) and replication of the same strain within RAW264.7 macrophages ( B ). The data are the mean values ± standard deviations of three biological replicates. Statistical significance (**** p < 0.0001; *** p < 0.001; ** p < 0.01) was determined by one-way ANOVA and the Tukey´s post-test.

Article Snippet: HeLa and RAW264.7 cells (Cell Lines Service (CLS), Heidelberg, Germany) were propagated in a high-glucose (4.5 g/L) Dulbecco’s modified Eagle’s medium (DMEM) containing 4 mM glutamine (PAA, Cölbe, Germany) and 10% (HeLa) or 6% (RAW264.7) inactivated fetal calf serum (iFCS) (Sigma), at 37 °C in an atmosphere of 5% CO 2 .

Techniques: Clone Assay