ratp Promega Search Results


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  • 91
    Promega ratp
    Ratp, supplied by Promega, used in various techniques. Bioz Stars score: 91/100, based on 571 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 91 stars, based on 571 article reviews
    Price from $9.99 to $1999.99
    ratp - by Bioz Stars, 2020-08
    91/100 stars
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    93
    Promega ratp standard
    Ratp Standard, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 3 article reviews
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    91
    Promega m ratp
    M Ratp, supplied by Promega, used in various techniques. Bioz Stars score: 91/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 91 stars, based on 13 article reviews
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    93
    Promega ribonucleotide adenosintriphosphates ratps
    Ribonucleotide Adenosintriphosphates Ratps, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Promega promega cat
    Promega Cat, supplied by Promega, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Promega rntp
    Rntp, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 149 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 149 article reviews
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    89
    Promega ntp mix
    Ntp Mix, supplied by Promega, used in various techniques. Bioz Stars score: 89/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Promega ligase mastermix
    Ligase Mastermix, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Promega ribonucleotide triphosphates
    Ribonucleotide Triphosphates, supplied by Promega, used in various techniques. Bioz Stars score: 95/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Promega nucleotide triphosphate mix
    Nucleotide Triphosphate Mix, supplied by Promega, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Promega kinase glo kit
    Kinase Glo Kit, supplied by Promega, used in various techniques. Bioz Stars score: 88/100, based on 56 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Promega magnesium acetate
    Magnesium Acetate, supplied by Promega, used in various techniques. Bioz Stars score: 94/100, based on 447 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Promega kinase glo luciferase kit
    Kinase Glo Luciferase Kit, supplied by Promega, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Promega bioluminescence assay purified nanoluc
    (A) Antibody libraries were created by cloning of assembled VH and VL genes after restriction digest with Nco I and Mlu I into phagemid vector pPL101. Cloned scFv were preceded by the pelB signal sequence and VH and VL genes were joined by a linker sequence (L1). The scFv was finally fused to the “short”(Δ) M13 gene III (corresponding to amino acids 251–406). After panning, the enriched phagemid population was digested by Nco I and Mlu I and subcloned into the expression vectors pPL302 and pPL303. Deduced amino acid sequences are shown for the most relevant fusion sites including linker sequences L1 (QLKSSGSGSESKST), L2 (GGGGSMEARLPAWQSGTVGKAT), L3 (GGGSCYKDDDDKGGGGS), as well as for the anti-gIII ( ATDYGAAIDGF) and the Strep-tag TM epitope ( WSHPQFEK ). (B) For the pIgG Nluc vector the <t>NanoLuc</t> gene was fused downstream to the heavy chain IgG1 CH3 gene domain including the linker sequence L4. Deduced amino acid sequences at the fusion site are shown for the linker L4 (GKGGGGS) and three N- and C-terminal amino acids of NanoLuc (MVF…[Nluc]…ILA). Genes for the heavy chain variable region, the constant regions and the signal peptide are depicted as VH, CH1-3 and S.
    Bioluminescence Assay Purified Nanoluc, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Promega ultra glo recombinant luciferase
    (A) Antibody libraries were created by cloning of assembled VH and VL genes after restriction digest with Nco I and Mlu I into phagemid vector pPL101. Cloned scFv were preceded by the pelB signal sequence and VH and VL genes were joined by a linker sequence (L1). The scFv was finally fused to the “short”(Δ) M13 gene III (corresponding to amino acids 251–406). After panning, the enriched phagemid population was digested by Nco I and Mlu I and subcloned into the expression vectors pPL302 and pPL303. Deduced amino acid sequences are shown for the most relevant fusion sites including linker sequences L1 (QLKSSGSGSESKST), L2 (GGGGSMEARLPAWQSGTVGKAT), L3 (GGGSCYKDDDDKGGGGS), as well as for the anti-gIII ( ATDYGAAIDGF) and the Strep-tag TM epitope ( WSHPQFEK ). (B) For the pIgG Nluc vector the <t>NanoLuc</t> gene was fused downstream to the heavy chain IgG1 CH3 gene domain including the linker sequence L4. Deduced amino acid sequences at the fusion site are shown for the linker L4 (GKGGGGS) and three N- and C-terminal amino acids of NanoLuc (MVF…[Nluc]…ILA). Genes for the heavy chain variable region, the constant regions and the signal peptide are depicted as VH, CH1-3 and S.
    Ultra Glo Recombinant Luciferase, supplied by Promega, used in various techniques. Bioz Stars score: 86/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Promega luciferase
    (A) Antibody libraries were created by cloning of assembled VH and VL genes after restriction digest with Nco I and Mlu I into phagemid vector pPL101. Cloned scFv were preceded by the pelB signal sequence and VH and VL genes were joined by a linker sequence (L1). The scFv was finally fused to the “short”(Δ) M13 gene III (corresponding to amino acids 251–406). After panning, the enriched phagemid population was digested by Nco I and Mlu I and subcloned into the expression vectors pPL302 and pPL303. Deduced amino acid sequences are shown for the most relevant fusion sites including linker sequences L1 (QLKSSGSGSESKST), L2 (GGGGSMEARLPAWQSGTVGKAT), L3 (GGGSCYKDDDDKGGGGS), as well as for the anti-gIII ( ATDYGAAIDGF) and the Strep-tag TM epitope ( WSHPQFEK ). (B) For the pIgG Nluc vector the <t>NanoLuc</t> gene was fused downstream to the heavy chain IgG1 CH3 gene domain including the linker sequence L4. Deduced amino acid sequences at the fusion site are shown for the linker L4 (GKGGGGS) and three N- and C-terminal amino acids of NanoLuc (MVF…[Nluc]…ILA). Genes for the heavy chain variable region, the constant regions and the signal peptide are depicted as VH, CH1-3 and S.
    Luciferase, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 4346 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Promega kinase glotm
    (A) Antibody libraries were created by cloning of assembled VH and VL genes after restriction digest with Nco I and Mlu I into phagemid vector pPL101. Cloned scFv were preceded by the pelB signal sequence and VH and VL genes were joined by a linker sequence (L1). The scFv was finally fused to the “short”(Δ) M13 gene III (corresponding to amino acids 251–406). After panning, the enriched phagemid population was digested by Nco I and Mlu I and subcloned into the expression vectors pPL302 and pPL303. Deduced amino acid sequences are shown for the most relevant fusion sites including linker sequences L1 (QLKSSGSGSESKST), L2 (GGGGSMEARLPAWQSGTVGKAT), L3 (GGGSCYKDDDDKGGGGS), as well as for the anti-gIII ( ATDYGAAIDGF) and the Strep-tag TM epitope ( WSHPQFEK ). (B) For the pIgG Nluc vector the <t>NanoLuc</t> gene was fused downstream to the heavy chain IgG1 CH3 gene domain including the linker sequence L4. Deduced amino acid sequences at the fusion site are shown for the linker L4 (GKGGGGS) and three N- and C-terminal amino acids of NanoLuc (MVF…[Nluc]…ILA). Genes for the heavy chain variable region, the constant regions and the signal peptide are depicted as VH, CH1-3 and S.
    Kinase Glotm, supplied by Promega, used in various techniques. Bioz Stars score: 86/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Promega quantilum recombinant luciferase
    (A) Antibody libraries were created by cloning of assembled VH and VL genes after restriction digest with Nco I and Mlu I into phagemid vector pPL101. Cloned scFv were preceded by the pelB signal sequence and VH and VL genes were joined by a linker sequence (L1). The scFv was finally fused to the “short”(Δ) M13 gene III (corresponding to amino acids 251–406). After panning, the enriched phagemid population was digested by Nco I and Mlu I and subcloned into the expression vectors pPL302 and pPL303. Deduced amino acid sequences are shown for the most relevant fusion sites including linker sequences L1 (QLKSSGSGSESKST), L2 (GGGGSMEARLPAWQSGTVGKAT), L3 (GGGSCYKDDDDKGGGGS), as well as for the anti-gIII ( ATDYGAAIDGF) and the Strep-tag TM epitope ( WSHPQFEK ). (B) For the pIgG Nluc vector the <t>NanoLuc</t> gene was fused downstream to the heavy chain IgG1 CH3 gene domain including the linker sequence L4. Deduced amino acid sequences at the fusion site are shown for the linker L4 (GKGGGGS) and three N- and C-terminal amino acids of NanoLuc (MVF…[Nluc]…ILA). Genes for the heavy chain variable region, the constant regions and the signal peptide are depicted as VH, CH1-3 and S.
    Quantilum Recombinant Luciferase, supplied by Promega, used in various techniques. Bioz Stars score: 91/100, based on 371 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Promega kinase glo based assay
    (A) Antibody libraries were created by cloning of assembled VH and VL genes after restriction digest with Nco I and Mlu I into phagemid vector pPL101. Cloned scFv were preceded by the pelB signal sequence and VH and VL genes were joined by a linker sequence (L1). The scFv was finally fused to the “short”(Δ) M13 gene III (corresponding to amino acids 251–406). After panning, the enriched phagemid population was digested by Nco I and Mlu I and subcloned into the expression vectors pPL302 and pPL303. Deduced amino acid sequences are shown for the most relevant fusion sites including linker sequences L1 (QLKSSGSGSESKST), L2 (GGGGSMEARLPAWQSGTVGKAT), L3 (GGGSCYKDDDDKGGGGS), as well as for the anti-gIII ( ATDYGAAIDGF) and the Strep-tag TM epitope ( WSHPQFEK ). (B) For the pIgG Nluc vector the <t>NanoLuc</t> gene was fused downstream to the heavy chain IgG1 CH3 gene domain including the linker sequence L4. Deduced amino acid sequences at the fusion site are shown for the linker L4 (GKGGGGS) and three N- and C-terminal amino acids of NanoLuc (MVF…[Nluc]…ILA). Genes for the heavy chain variable region, the constant regions and the signal peptide are depicted as VH, CH1-3 and S.
    Kinase Glo Based Assay, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Promega kinase glo luciferase assay
    (A) Antibody libraries were created by cloning of assembled VH and VL genes after restriction digest with Nco I and Mlu I into phagemid vector pPL101. Cloned scFv were preceded by the pelB signal sequence and VH and VL genes were joined by a linker sequence (L1). The scFv was finally fused to the “short”(Δ) M13 gene III (corresponding to amino acids 251–406). After panning, the enriched phagemid population was digested by Nco I and Mlu I and subcloned into the expression vectors pPL302 and pPL303. Deduced amino acid sequences are shown for the most relevant fusion sites including linker sequences L1 (QLKSSGSGSESKST), L2 (GGGGSMEARLPAWQSGTVGKAT), L3 (GGGSCYKDDDDKGGGGS), as well as for the anti-gIII ( ATDYGAAIDGF) and the Strep-tag TM epitope ( WSHPQFEK ). (B) For the pIgG Nluc vector the <t>NanoLuc</t> gene was fused downstream to the heavy chain IgG1 CH3 gene domain including the linker sequence L4. Deduced amino acid sequences at the fusion site are shown for the linker L4 (GKGGGGS) and three N- and C-terminal amino acids of NanoLuc (MVF…[Nluc]…ILA). Genes for the heavy chain variable region, the constant regions and the signal peptide are depicted as VH, CH1-3 and S.
    Kinase Glo Luciferase Assay, supplied by Promega, used in various techniques. Bioz Stars score: 86/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Promega cdk2 cyclin a2 kinase enzyme system
    (A) Antibody libraries were created by cloning of assembled VH and VL genes after restriction digest with Nco I and Mlu I into phagemid vector pPL101. Cloned scFv were preceded by the pelB signal sequence and VH and VL genes were joined by a linker sequence (L1). The scFv was finally fused to the “short”(Δ) M13 gene III (corresponding to amino acids 251–406). After panning, the enriched phagemid population was digested by Nco I and Mlu I and subcloned into the expression vectors pPL302 and pPL303. Deduced amino acid sequences are shown for the most relevant fusion sites including linker sequences L1 (QLKSSGSGSESKST), L2 (GGGGSMEARLPAWQSGTVGKAT), L3 (GGGSCYKDDDDKGGGGS), as well as for the anti-gIII ( ATDYGAAIDGF) and the Strep-tag TM epitope ( WSHPQFEK ). (B) For the pIgG Nluc vector the <t>NanoLuc</t> gene was fused downstream to the heavy chain IgG1 CH3 gene domain including the linker sequence L4. Deduced amino acid sequences at the fusion site are shown for the linker L4 (GKGGGGS) and three N- and C-terminal amino acids of NanoLuc (MVF…[Nluc]…ILA). Genes for the heavy chain variable region, the constant regions and the signal peptide are depicted as VH, CH1-3 and S.
    Cdk2 Cyclin A2 Kinase Enzyme System, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Promega gsk 3β assay kinase glo kit
    Linear graph of IC 50 assay of <t>GSK-3β</t> treated with selected Amaryllidaceae alkaloids. Concentrations of alkaloids were 6.25; 12.5; 25; 50 and 100 μM. Activity is presented as % inhibition.
    Gsk 3β Assay Kinase Glo Kit, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    atp  (Promega)
    93
    Promega atp
    Linear graph of IC 50 assay of <t>GSK-3β</t> treated with selected Amaryllidaceae alkaloids. Concentrations of alkaloids were 6.25; 12.5; 25; 50 and 100 μM. Activity is presented as % inhibition.
    Atp, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 1722 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    (A) Antibody libraries were created by cloning of assembled VH and VL genes after restriction digest with Nco I and Mlu I into phagemid vector pPL101. Cloned scFv were preceded by the pelB signal sequence and VH and VL genes were joined by a linker sequence (L1). The scFv was finally fused to the “short”(Δ) M13 gene III (corresponding to amino acids 251–406). After panning, the enriched phagemid population was digested by Nco I and Mlu I and subcloned into the expression vectors pPL302 and pPL303. Deduced amino acid sequences are shown for the most relevant fusion sites including linker sequences L1 (QLKSSGSGSESKST), L2 (GGGGSMEARLPAWQSGTVGKAT), L3 (GGGSCYKDDDDKGGGGS), as well as for the anti-gIII ( ATDYGAAIDGF) and the Strep-tag TM epitope ( WSHPQFEK ). (B) For the pIgG Nluc vector the NanoLuc gene was fused downstream to the heavy chain IgG1 CH3 gene domain including the linker sequence L4. Deduced amino acid sequences at the fusion site are shown for the linker L4 (GKGGGGS) and three N- and C-terminal amino acids of NanoLuc (MVF…[Nluc]…ILA). Genes for the heavy chain variable region, the constant regions and the signal peptide are depicted as VH, CH1-3 and S.

    Journal: Frontiers in Pharmacology

    Article Title: NanoLuc Luciferase – A Multifunctional Tool for High Throughput Antibody Screening

    doi: 10.3389/fphar.2016.00027

    Figure Lengend Snippet: (A) Antibody libraries were created by cloning of assembled VH and VL genes after restriction digest with Nco I and Mlu I into phagemid vector pPL101. Cloned scFv were preceded by the pelB signal sequence and VH and VL genes were joined by a linker sequence (L1). The scFv was finally fused to the “short”(Δ) M13 gene III (corresponding to amino acids 251–406). After panning, the enriched phagemid population was digested by Nco I and Mlu I and subcloned into the expression vectors pPL302 and pPL303. Deduced amino acid sequences are shown for the most relevant fusion sites including linker sequences L1 (QLKSSGSGSESKST), L2 (GGGGSMEARLPAWQSGTVGKAT), L3 (GGGSCYKDDDDKGGGGS), as well as for the anti-gIII ( ATDYGAAIDGF) and the Strep-tag TM epitope ( WSHPQFEK ). (B) For the pIgG Nluc vector the NanoLuc gene was fused downstream to the heavy chain IgG1 CH3 gene domain including the linker sequence L4. Deduced amino acid sequences at the fusion site are shown for the linker L4 (GKGGGGS) and three N- and C-terminal amino acids of NanoLuc (MVF…[Nluc]…ILA). Genes for the heavy chain variable region, the constant regions and the signal peptide are depicted as VH, CH1-3 and S.

    Article Snippet: Establishing Bioluminescence Assay Purified NanoLuc (Promega), was distributed in a 96-well half-area white microplate (Costar, CLS3693) to a final concentration of 10 pmol/L in PBS; PBS, 0.1% BSA; 50% Nano-Glo assay buffer (Promega, N112B) in PBS or 50% Nano-Glo assay buffer, 0.1% BSA with two different dilutions (1/100 and 1/400 final) of furimazine (Nano-GloTM assay substrate, Promega, N113B).

    Techniques: Clone Assay, Plasmid Preparation, Sequencing, Expressing, Strep-tag

    Purified NanoLuc glow kinetic in different buffer conditions. Luminescence signal of purified Nanoluc (10 pmol/L) was measured at several time points in a 20 μL final volume of several buffer conditions : PBS (circle), PBS, 0.1% BSA (square); 50% Nano-Glo assay buffer (Promega, N112B), 50% PBS (triangle) or 50% Nano-Glo assay buffer 50% PBS, 0.1% BSA (diamond) with 1/100 (white) or 1/400 (black) furimazine final dilution. Graphs of a representative experiment, each experimental point was performed in triplicate, and bars correspond to standard deviation (SD).

    Journal: Frontiers in Pharmacology

    Article Title: NanoLuc Luciferase – A Multifunctional Tool for High Throughput Antibody Screening

    doi: 10.3389/fphar.2016.00027

    Figure Lengend Snippet: Purified NanoLuc glow kinetic in different buffer conditions. Luminescence signal of purified Nanoluc (10 pmol/L) was measured at several time points in a 20 μL final volume of several buffer conditions : PBS (circle), PBS, 0.1% BSA (square); 50% Nano-Glo assay buffer (Promega, N112B), 50% PBS (triangle) or 50% Nano-Glo assay buffer 50% PBS, 0.1% BSA (diamond) with 1/100 (white) or 1/400 (black) furimazine final dilution. Graphs of a representative experiment, each experimental point was performed in triplicate, and bars correspond to standard deviation (SD).

    Article Snippet: Establishing Bioluminescence Assay Purified NanoLuc (Promega), was distributed in a 96-well half-area white microplate (Costar, CLS3693) to a final concentration of 10 pmol/L in PBS; PBS, 0.1% BSA; 50% Nano-Glo assay buffer (Promega, N112B) in PBS or 50% Nano-Glo assay buffer, 0.1% BSA with two different dilutions (1/100 and 1/400 final) of furimazine (Nano-GloTM assay substrate, Promega, N113B).

    Techniques: Purification, Glo Assay, Standard Deviation

    Linear graph of IC 50 assay of GSK-3β treated with selected Amaryllidaceae alkaloids. Concentrations of alkaloids were 6.25; 12.5; 25; 50 and 100 μM. Activity is presented as % inhibition.

    Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

    Article Title: Amaryllidaceae Alkaloids as Potential Glycogen Synthase Kinase-3β Inhibitors

    doi: 10.3390/molecules23040719

    Figure Lengend Snippet: Linear graph of IC 50 assay of GSK-3β treated with selected Amaryllidaceae alkaloids. Concentrations of alkaloids were 6.25; 12.5; 25; 50 and 100 μM. Activity is presented as % inhibition.

    Article Snippet: GSK-3β Assay Kinase-Glo Kit was obtained from Promega (Promega Biotech Iberica, S.L., Madrid, Spain), and human recombinant GSK-3β and GSM substrate mimicking Glycogen Muscle Synthase from Merck Millipore (Darmstadt, Germany).

    Techniques: Activity Assay, Inhibition