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  • 99
    Millipore adenosine triphosphates ratp
    Adenosine Triphosphates Ratp, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Promega ratp
    Ratp, supplied by Promega, used in various techniques. Bioz Stars score: 91/100, based on 571 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Thermo Fisher ratp
    Ratp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 131 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies ratp
    Ratp, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Millipore ratp
    Ratp, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Promega ratp standard
    Ratp Standard, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    GE Healthcare ratp pharmacia amersham
    Ratp Pharmacia Amersham, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 85/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    GE Healthcare γ 32 p ratp
    γ 32 P Ratp, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 85/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PerkinElmer α labeled ratp
    α Labeled Ratp, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Promega m ratp
    M Ratp, supplied by Promega, used in various techniques. Bioz Stars score: 91/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Millipore mu recombinant atp sulfurylase
    Bioluminescence regenerative cycle (BRC) with <t>ATP-sulfurylase</t> and firefly luciferase detecting the released pyrophosphate from nucleic acid polymerization.
    Mu Recombinant Atp Sulfurylase, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PerkinElmer α 32 p ratp
    Bioluminescence regenerative cycle (BRC) with <t>ATP-sulfurylase</t> and firefly luciferase detecting the released pyrophosphate from nucleic acid polymerization.
    α 32 P Ratp, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 85/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    TriLink 2 oh ratp
    Bioluminescence regenerative cycle (BRC) with <t>ATP-sulfurylase</t> and firefly luciferase detecting the released pyrophosphate from nucleic acid polymerization.
    2 Oh Ratp, supplied by TriLink, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Valiant γ 32 p ratp
    Bioluminescence regenerative cycle (BRC) with <t>ATP-sulfurylase</t> and firefly luciferase detecting the released pyrophosphate from nucleic acid polymerization.
    γ 32 P Ratp, supplied by Valiant, used in various techniques. Bioz Stars score: 86/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Promega ribonucleotide adenosintriphosphates ratps
    Bioluminescence regenerative cycle (BRC) with <t>ATP-sulfurylase</t> and firefly luciferase detecting the released pyrophosphate from nucleic acid polymerization.
    Ribonucleotide Adenosintriphosphates Ratps, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Bioluminescence regenerative cycle (BRC) with ATP-sulfurylase and firefly luciferase detecting the released pyrophosphate from nucleic acid polymerization.

    Journal:

    Article Title: Bioluminescence Regenerative Cycle (BRC) System for Nucleic Acid Quantification Assays

    doi: 10.1016/j.bpc.2005.04.002

    Figure Lengend Snippet: Bioluminescence regenerative cycle (BRC) with ATP-sulfurylase and firefly luciferase detecting the released pyrophosphate from nucleic acid polymerization.

    Article Snippet: The 40µl bioluminescence assay mixture contained: 3.0 µg luciferase (Promega, USA), 50 mU recombinant ATP-sulfurylase (Sigma, USA), 0.1 M Tris-acetate (pH 7.75), 0.5 mM EDTA (Sigma, USA), 5 mM Mg-acetate (Sigma, USA), 0.1% (w/v) bovine serum albumin (Sigma, USA), 2.5 mM dithiothreitol (Sigma, USA), 10 µM adenosine 5’-phosphosulfate (APS) (Biolog, Belgium), 0.4 mg polyvinylpyrrolidone/ml (molecular weight 360000) and 100 µg D-luciferin/ml (BioThema, Uppsal, Sweden), and the emitted light was detected in real time and measured after approximately 45 sec with 1 sec and 10 sec integration times for the CCD imaging system and luminometer, respectively.

    Techniques: Luciferase