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rapid hybridization buffer  (Danaher Inc)
86
Danaher Inc rapid hybridization buffer
Figure 3. p53-dependent repression of MCM5 and E2F1. A, total RNA was isolated from O41 and TR9-7 cells growing in the presence or absence of tetracycline (tet). The RNA was used for <t>hybridization</t> with probes for MCM5 (top) and GAPDH (bottom). Bottom of lane, fold induction of MCM5 as normalized with GAPDH. B, tetracycline was withdrawn from TR9-7ER cells for the indicated time. Total cell lysates were used for Western blotting for p53, p21, MCM5, E2F1, and actin.
Rapid Hybridization Buffer, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rapid hybridization buffer - by Bioz Stars, 2026-05
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Figure 3. p53-dependent repression of MCM5 and E2F1. A, total RNA was isolated from O41 and TR9-7 cells growing in the presence or absence of tetracycline (tet). The RNA was used for hybridization with probes for MCM5 (top) and GAPDH (bottom). Bottom of lane, fold induction of MCM5 as normalized with GAPDH. B, tetracycline was withdrawn from TR9-7ER cells for the indicated time. Total cell lysates were used for Western blotting for p53, p21, MCM5, E2F1, and actin.

Journal: Cancer Research

Article Title: DNA Replication Licensing Factor Minichromosome Maintenance Deficient 5 Rescues p53-Mediated Growth Arrest

doi: 10.1158/0008-5472.can-06-2835

Figure Lengend Snippet: Figure 3. p53-dependent repression of MCM5 and E2F1. A, total RNA was isolated from O41 and TR9-7 cells growing in the presence or absence of tetracycline (tet). The RNA was used for hybridization with probes for MCM5 (top) and GAPDH (bottom). Bottom of lane, fold induction of MCM5 as normalized with GAPDH. B, tetracycline was withdrawn from TR9-7ER cells for the indicated time. Total cell lysates were used for Western blotting for p53, p21, MCM5, E2F1, and actin.

Article Snippet: The filter was hybridized with 32P-labeled cDNA probes in a rapid hybridization buffer (Amersham Pharmacia Biotech).

Techniques: Isolation, Hybridization, Western Blot