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90
Bioss p nf κb p65
EA can inhibit the activation of microglia in the pPD stage mediated by TLR2. ( A ) Western blot detection of TLR2 protein expression in the substantia nigra. ( B ) Western blot detection of MyD88 protein expression in the substantia nigra. ( C ) Western blot detection <t>of</t> <t>p-NF-κB-p65</t> expression in the substantia nigra. ( D ) Western blot detection of NLRP3 protein expression in the substantia nigra. ( E ) Western blot detection of Caspase-1 protein expression in the substantia nigra. ( F ) Western blot detection of GSDMD protein expression in the substantia nigra. ( G ) Western blot detection of IL-1β protein expression in the substantia nigra. (n=6; compared with the control group, *** P <0.001; compared with the model group, ### P <0.001, ## P <0.01, # P <0.05).
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R&D Systems recombinant mouse rankl
Fig. 1. PSCs promote osteoclast differentiation. (A and B) BMMs were grouped to induce differentiation under different interventions and osteoclast formation was detected by TRAP staining. (C and D) Actin ring formation in response to different stimuli. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 vs the M-CSF + <t>RANKL</t> group. Data are presented as the mean ± SD of three independent experiments. The scale bar is 500 μm.
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R&D Systems mouse soluble rankl
Fig. 1. PSCs promote osteoclast differentiation. (A and B) BMMs were grouped to induce differentiation under different interventions and osteoclast formation was detected by TRAP staining. (C and D) Actin ring formation in response to different stimuli. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 vs the M-CSF + <t>RANKL</t> group. Data are presented as the mean ± SD of three independent experiments. The scale bar is 500 μm.
Mouse Soluble Rankl, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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R&D Systems rankl mab626
Fig. 1. PSCs promote osteoclast differentiation. (A and B) BMMs were grouped to induce differentiation under different interventions and osteoclast formation was detected by TRAP staining. (C and D) Actin ring formation in response to different stimuli. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 vs the M-CSF + <t>RANKL</t> group. Data are presented as the mean ± SD of three independent experiments. The scale bar is 500 μm.
Rankl Mab626, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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R&D Systems quantikine elisa
Fig. 1. PSCs promote osteoclast differentiation. (A and B) BMMs were grouped to induce differentiation under different interventions and osteoclast formation was detected by TRAP staining. (C and D) Actin ring formation in response to different stimuli. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 vs the M-CSF + <t>RANKL</t> group. Data are presented as the mean ± SD of three independent experiments. The scale bar is 500 μm.
Quantikine Elisa, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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96
R&D Systems factor kappa b ligand
Fig. 1. PSCs promote osteoclast differentiation. (A and B) BMMs were grouped to induce differentiation under different interventions and osteoclast formation was detected by TRAP staining. (C and D) Actin ring formation in response to different stimuli. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 vs the M-CSF + <t>RANKL</t> group. Data are presented as the mean ± SD of three independent experiments. The scale bar is 500 μm.
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91
R&D Systems anti human trance tnfsf11 antibody
Fig. 1. PSCs promote osteoclast differentiation. (A and B) BMMs were grouped to induce differentiation under different interventions and osteoclast formation was detected by TRAP staining. (C and D) Actin ring formation in response to different stimuli. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 vs the M-CSF + <t>RANKL</t> group. Data are presented as the mean ± SD of three independent experiments. The scale bar is 500 μm.
Anti Human Trance Tnfsf11 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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92
Biorbyt anti rank antibody
Fig. 1. PSCs promote osteoclast differentiation. (A and B) BMMs were grouped to induce differentiation under different interventions and osteoclast formation was detected by TRAP staining. (C and D) Actin ring formation in response to different stimuli. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 vs the M-CSF + <t>RANKL</t> group. Data are presented as the mean ± SD of three independent experiments. The scale bar is 500 μm.
Anti Rank Antibody, supplied by Biorbyt, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Santa Cruz Biotechnology hurank lentiviral activation particles
Fig. 1. PSCs promote osteoclast differentiation. (A and B) BMMs were grouped to induce differentiation under different interventions and osteoclast formation was detected by TRAP staining. (C and D) Actin ring formation in response to different stimuli. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 vs the M-CSF + <t>RANKL</t> group. Data are presented as the mean ± SD of three independent experiments. The scale bar is 500 μm.
Hurank Lentiviral Activation Particles, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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94
Santa Cruz Biotechnology anti rank h 7
Fig. 1. PSCs promote osteoclast differentiation. (A and B) BMMs were grouped to induce differentiation under different interventions and osteoclast formation was detected by TRAP staining. (C and D) Actin ring formation in response to different stimuli. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 vs the M-CSF + <t>RANKL</t> group. Data are presented as the mean ± SD of three independent experiments. The scale bar is 500 μm.
Anti Rank H 7, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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92
ABclonal Biotechnology rankl
Fig. 1. PSCs promote osteoclast differentiation. (A and B) BMMs were grouped to induce differentiation under different interventions and osteoclast formation was detected by TRAP staining. (C and D) Actin ring formation in response to different stimuli. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 vs the M-CSF + <t>RANKL</t> group. Data are presented as the mean ± SD of three independent experiments. The scale bar is 500 μm.
Rankl, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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94
R&D Systems biological chemistry 8083 ferentiation medium
Fig. 1. PSCs promote osteoclast differentiation. (A and B) BMMs were grouped to induce differentiation under different interventions and osteoclast formation was detected by TRAP staining. (C and D) Actin ring formation in response to different stimuli. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 vs the M-CSF + <t>RANKL</t> group. Data are presented as the mean ± SD of three independent experiments. The scale bar is 500 μm.
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Image Search Results


EA can inhibit the activation of microglia in the pPD stage mediated by TLR2. ( A ) Western blot detection of TLR2 protein expression in the substantia nigra. ( B ) Western blot detection of MyD88 protein expression in the substantia nigra. ( C ) Western blot detection of p-NF-κB-p65 expression in the substantia nigra. ( D ) Western blot detection of NLRP3 protein expression in the substantia nigra. ( E ) Western blot detection of Caspase-1 protein expression in the substantia nigra. ( F ) Western blot detection of GSDMD protein expression in the substantia nigra. ( G ) Western blot detection of IL-1β protein expression in the substantia nigra. (n=6; compared with the control group, *** P <0.001; compared with the model group, ### P <0.001, ## P <0.01, # P <0.05).

Journal: Journal of Inflammation Research

Article Title: Electroacupuncture Inhibits the Early Neuroinflammatory Cascade Triggered by TLR2 in the Prodromal Period of PD

doi: 10.2147/JIR.S585729

Figure Lengend Snippet: EA can inhibit the activation of microglia in the pPD stage mediated by TLR2. ( A ) Western blot detection of TLR2 protein expression in the substantia nigra. ( B ) Western blot detection of MyD88 protein expression in the substantia nigra. ( C ) Western blot detection of p-NF-κB-p65 expression in the substantia nigra. ( D ) Western blot detection of NLRP3 protein expression in the substantia nigra. ( E ) Western blot detection of Caspase-1 protein expression in the substantia nigra. ( F ) Western blot detection of GSDMD protein expression in the substantia nigra. ( G ) Western blot detection of IL-1β protein expression in the substantia nigra. (n=6; compared with the control group, *** P <0.001; compared with the model group, ### P <0.001, ## P <0.01, # P <0.05).

Article Snippet: The membrane was incubated overnight at 4°C with primary antibodies specific to TLR2 (#bs-1019R, Bioss, 1:1000), MyD88 (#bs-1047R, Bioss, 1:1000), p-NF-κB-p65 (#bs-7343R, Bioss, 1:1000), NLRP3 (#bs-41293R, Bioss, 1:1000), caspase-1 (#bsm-52441R, Bioss, 1:1000), IL-1β (#bs-0812R, Bioss, 1:1000), and GSDMD (#bs-14287R, Bioss, 1:1000), followed by incubation with an IgG secondary antibody (#bs-10900R, Bioss, 1:5000) for 1 hour in the dark.

Techniques: Activation Assay, Western Blot, Expressing, Control

EA intervention affects the expression of TLR2 in different regions of pPD, inhibits microglial activation, regulates the TLR2/NF-κB/NLRP3 pathway, and suppresses pyroptosis of microglia.

Journal: Journal of Inflammation Research

Article Title: Electroacupuncture Inhibits the Early Neuroinflammatory Cascade Triggered by TLR2 in the Prodromal Period of PD

doi: 10.2147/JIR.S585729

Figure Lengend Snippet: EA intervention affects the expression of TLR2 in different regions of pPD, inhibits microglial activation, regulates the TLR2/NF-κB/NLRP3 pathway, and suppresses pyroptosis of microglia.

Article Snippet: The membrane was incubated overnight at 4°C with primary antibodies specific to TLR2 (#bs-1019R, Bioss, 1:1000), MyD88 (#bs-1047R, Bioss, 1:1000), p-NF-κB-p65 (#bs-7343R, Bioss, 1:1000), NLRP3 (#bs-41293R, Bioss, 1:1000), caspase-1 (#bsm-52441R, Bioss, 1:1000), IL-1β (#bs-0812R, Bioss, 1:1000), and GSDMD (#bs-14287R, Bioss, 1:1000), followed by incubation with an IgG secondary antibody (#bs-10900R, Bioss, 1:5000) for 1 hour in the dark.

Techniques: Expressing, Activation Assay

Fig. 1. PSCs promote osteoclast differentiation. (A and B) BMMs were grouped to induce differentiation under different interventions and osteoclast formation was detected by TRAP staining. (C and D) Actin ring formation in response to different stimuli. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 vs the M-CSF + RANKL group. Data are presented as the mean ± SD of three independent experiments. The scale bar is 500 μm.

Journal: Acta tropica

Article Title: Echinococcus granulosus promotes bone resorption by increasing osteoclasts differentiation.

doi: 10.1016/j.actatropica.2023.107027

Figure Lengend Snippet: Fig. 1. PSCs promote osteoclast differentiation. (A and B) BMMs were grouped to induce differentiation under different interventions and osteoclast formation was detected by TRAP staining. (C and D) Actin ring formation in response to different stimuli. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 vs the M-CSF + RANKL group. Data are presented as the mean ± SD of three independent experiments. The scale bar is 500 μm.

Article Snippet: Recombinant mouse M-CSF (#416-ML/CF) and recombinant mouse RANKL (#462- TEC) were purchased from R&D Systems (Minnesota, USA).

Techniques: Staining

Fig. 2. PSCs promote the expression of osteoclast-related proteins and genes. (A) BMMs were cultured for 24 h using the same intervention method, total RNA was extracted, and the target gene was detected. (B and C) The expression of target proteins was detected between the groups. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 vs the M-CSF + RANKL group. #P < 0.05, ##P < 0.01, ###P < 0.001, ####P < 0.0001 vs the M-CSF group. Data are presented as the mean ± SD of three independent experiments.

Journal: Acta tropica

Article Title: Echinococcus granulosus promotes bone resorption by increasing osteoclasts differentiation.

doi: 10.1016/j.actatropica.2023.107027

Figure Lengend Snippet: Fig. 2. PSCs promote the expression of osteoclast-related proteins and genes. (A) BMMs were cultured for 24 h using the same intervention method, total RNA was extracted, and the target gene was detected. (B and C) The expression of target proteins was detected between the groups. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 vs the M-CSF + RANKL group. #P < 0.05, ##P < 0.01, ###P < 0.001, ####P < 0.0001 vs the M-CSF group. Data are presented as the mean ± SD of three independent experiments.

Article Snippet: Recombinant mouse M-CSF (#416-ML/CF) and recombinant mouse RANKL (#462- TEC) were purchased from R&D Systems (Minnesota, USA).

Techniques: Expressing, Cell Culture