random hexanucleotide primers Search Results


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  • 99
    Thermo Fisher hexanucleotide random primers
    Hexanucleotide Random Primers, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 85 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Millipore random hexanucleotide primers
    Random Hexanucleotide Primers, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega oligo dt und hexanukleotide random primers
    Oligo Dt Und Hexanukleotide Random Primers, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche random hexanucleotide primers
    Random Hexanucleotide Primers, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 131 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Boehringer Mannheim random hexanucleotide primers
    Random Hexanucleotide Primers, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 91/100, based on 88 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare random hexanucleotide primers
    Random Hexanucleotide Primers, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 91/100, based on 78 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Meridian Life Science hexanucleotide random primers
    Hexanucleotide Random Primers, supplied by Meridian Life Science, used in various techniques. Bioz Stars score: 88/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Illumina Inc random hexanucleotide primers
    Random Hexanucleotide Primers, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa hexanucleotide random primers
    Hexanucleotide Random Primers, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher random hexanucleotide primers
    Deletion of pilA does not affect masB transcription. RNA was harvested from 1×10 8 cells and mRNA was used to generate cDNA using a random <t>hexanucleotide</t> primer. Gene specific primers for masB and the 16S gene (endogenous control) were used in the amplification reaction with cDNA. masB expression was amplified from cDNA using 50, 5 and 0.5 ng template, while 16 S expression was amplified using 200, 20 and 2 pg cDNA. Both the WT and pilA strain express masB (black band in lanes 1 and 4 respectively). Hence, deletion of pilA does not adversely affect the expression of masB in M. xanthus . Deletion of masABK abolished masB expression (lanes 7–9).
    Random Hexanucleotide Primers, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 627 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher dn6 random hexanucleotide primer
    Deletion of pilA does not affect masB transcription. RNA was harvested from 1×10 8 cells and mRNA was used to generate cDNA using a random <t>hexanucleotide</t> primer. Gene specific primers for masB and the 16S gene (endogenous control) were used in the amplification reaction with cDNA. masB expression was amplified from cDNA using 50, 5 and 0.5 ng template, while 16 S expression was amplified using 200, 20 and 2 pg cDNA. Both the WT and pilA strain express masB (black band in lanes 1 and 4 respectively). Hence, deletion of pilA does not adversely affect the expression of masB in M. xanthus . Deletion of masABK abolished masB expression (lanes 7–9).
    Dn6 Random Hexanucleotide Primer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Roche random hexanucleotide primer kit
    Deletion of pilA does not affect masB transcription. RNA was harvested from 1×10 8 cells and mRNA was used to generate cDNA using a random <t>hexanucleotide</t> primer. Gene specific primers for masB and the 16S gene (endogenous control) were used in the amplification reaction with cDNA. masB expression was amplified from cDNA using 50, 5 and 0.5 ng template, while 16 S expression was amplified using 200, 20 and 2 pg cDNA. Both the WT and pilA strain express masB (black band in lanes 1 and 4 respectively). Hence, deletion of pilA does not adversely affect the expression of masB in M. xanthus . Deletion of masABK abolished masB expression (lanes 7–9).
    Random Hexanucleotide Primer Kit, supplied by Roche, used in various techniques. Bioz Stars score: 88/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Deletion of pilA does not affect masB transcription. RNA was harvested from 1×10 8 cells and mRNA was used to generate cDNA using a random hexanucleotide primer. Gene specific primers for masB and the 16S gene (endogenous control) were used in the amplification reaction with cDNA. masB expression was amplified from cDNA using 50, 5 and 0.5 ng template, while 16 S expression was amplified using 200, 20 and 2 pg cDNA. Both the WT and pilA strain express masB (black band in lanes 1 and 4 respectively). Hence, deletion of pilA does not adversely affect the expression of masB in M. xanthus . Deletion of masABK abolished masB expression (lanes 7–9).

    Journal: PLoS ONE

    Article Title: MasABK Proteins Interact with Proteins of the Type IV Pilin System to Affect Social Motility of Myxococcus xanthus

    doi: 10.1371/journal.pone.0054557

    Figure Lengend Snippet: Deletion of pilA does not affect masB transcription. RNA was harvested from 1×10 8 cells and mRNA was used to generate cDNA using a random hexanucleotide primer. Gene specific primers for masB and the 16S gene (endogenous control) were used in the amplification reaction with cDNA. masB expression was amplified from cDNA using 50, 5 and 0.5 ng template, while 16 S expression was amplified using 200, 20 and 2 pg cDNA. Both the WT and pilA strain express masB (black band in lanes 1 and 4 respectively). Hence, deletion of pilA does not adversely affect the expression of masB in M. xanthus . Deletion of masABK abolished masB expression (lanes 7–9).

    Article Snippet: Double-stranded cDNAs were synthesized using Invitrogen's SuperScript cDNA Synthesis Kit with the standard protocol using random hexanucleotide primers (Invitrogen, Carlsbad, CA).

    Techniques: Amplification, Expressing