ramos Search Results


ramos cells  (ATCC)
99
ATCC ramos cells
Ramos Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ramos cell  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology ramos cell
Figure 2. Inhibition of NFATc1 in MC38Met cells. Effect of control RNAi (siScr) or NFATc1-specific RNAi on specific NFAT mRNA species: NFATc1 (A), NFATc2 (B), NFATc3 (C). D, the effect of NFATc1 siRNA versus scrambled control si-Scr on NFAT family protein levels in MC38Met cells, and steady state levels in both MC38Par and MC38Met cells with <t>Ramos</t> <t>cell</t> lysate positive control in the right hand lane. E, relative rates of invasion for cells shown in A–D . , P < 0.0005; , P < 0.00005.
Ramos Cell, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tx sc  (Santa Cruz Biotechnology)
95
Santa Cruz Biotechnology tx sc
Figure 2. Inhibition of NFATc1 in MC38Met cells. Effect of control RNAi (siScr) or NFATc1-specific RNAi on specific NFAT mRNA species: NFATc1 (A), NFATc2 (B), NFATc3 (C). D, the effect of NFATc1 siRNA versus scrambled control si-Scr on NFAT family protein levels in MC38Met cells, and steady state levels in both MC38Par and MC38Met cells with <t>Ramos</t> <t>cell</t> lysate positive control in the right hand lane. E, relative rates of invasion for cells shown in A–D . , P < 0.0005; , P < 0.00005.
Tx Sc, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ramos cell lysate  (Novus Biologicals)
90
Novus Biologicals ramos cell lysate
FIG. 2. Immunoblot analysis of TLR proteins in male reproductive organs. Aliquots (100 lg) of cytoplasmic protein extracts were separated by PAGE, electroblotted, and blots probed with anti-TLR antibodies followed by enhanced chemiluminescence detection using Pierce Super- Signal West Pico or Pierce SuperSignal West Femto (*F) substrate. Representative results are shown (n ¼ 3–5 rats). þ, control rat tissue extracts from spleen, TLRs 1–7; lung, TLR8; and small intestine, TLR9. þ Ext, positive-control whole cell lysates used were Raw 264 Abelson transformed macrophages (TLRs 1, 2–6, and 8–10); SW480 colorectal adenocarcinoma (TLR2); Daudi cell extract (TLR7); <t>Ramos</t> <t>cell</t> lysate (TLR10) and mouse heart whole cell lysate (TLR11). Blots were stripped and reprobed with anti-actin monoclonal antibody to detect actin as a loading control. Representative results are shown (n ¼ 3–5 rats) for blots exposed to film for the same length of time when using equivalent chemiluminescent substrate.
Ramos Cell Lysate, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ramos cell lysates  (Santa Cruz Biotechnology)
90
Santa Cruz Biotechnology ramos cell lysates
FIG. 2. NFAT4 is functionally active in glial cells. (a) NFAT expression in SVG-A and U-87MG whole-cell lysates. NFATs 1 to 4 were detected using antibodies (NFATc1 7A6, NFATc2 4G6-G5, NFATc3 F-1, and NFATc4 H-74; <t>Santa</t> <t>Cruz</t> Biotech Inc.) diluted 1:200. <t>Ramos</t> cell lysates were used as a positive control. (b) Luciferase reporter gene assays using an NFAT-responsive reporter construct were used to compare NFAT activity in SVG-A cells and U-87MG cells in the presence or absence of 292 mg/liter glutamate. A control construct that lacked the NFAT binding site was used to measure basal transcriptional activity. (c) The NFAT reporter construct was cotransfected with either a control construct ( SV40 T-Ag) or a construct expressing the SV40 large T-Ag ( SV40 T-Ag).
Ramos Cell Lysates, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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growth cell line origin source ramos burkitt lymphoma dsmz  (DSMZ)
94
DSMZ growth cell line origin source ramos burkitt lymphoma dsmz
FIG. 2. NFAT4 is functionally active in glial cells. (a) NFAT expression in SVG-A and U-87MG whole-cell lysates. NFATs 1 to 4 were detected using antibodies (NFATc1 7A6, NFATc2 4G6-G5, NFATc3 F-1, and NFATc4 H-74; <t>Santa</t> <t>Cruz</t> Biotech Inc.) diluted 1:200. <t>Ramos</t> cell lysates were used as a positive control. (b) Luciferase reporter gene assays using an NFAT-responsive reporter construct were used to compare NFAT activity in SVG-A cells and U-87MG cells in the presence or absence of 292 mg/liter glutamate. A control construct that lacked the NFAT binding site was used to measure basal transcriptional activity. (c) The NFAT reporter construct was cotransfected with either a control construct ( SV40 T-Ag) or a construct expressing the SV40 large T-Ag ( SV40 T-Ag).
Growth Cell Line Origin Source Ramos Burkitt Lymphoma Dsmz, supplied by DSMZ, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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seh  (Santa Cruz Biotechnology)
88
Santa Cruz Biotechnology seh
FIG. 2. NFAT4 is functionally active in glial cells. (a) NFAT expression in SVG-A and U-87MG whole-cell lysates. NFATs 1 to 4 were detected using antibodies (NFATc1 7A6, NFATc2 4G6-G5, NFATc3 F-1, and NFATc4 H-74; <t>Santa</t> <t>Cruz</t> Biotech Inc.) diluted 1:200. <t>Ramos</t> cell lysates were used as a positive control. (b) Luciferase reporter gene assays using an NFAT-responsive reporter construct were used to compare NFAT activity in SVG-A cells and U-87MG cells in the presence or absence of 292 mg/liter glutamate. A control construct that lacked the NFAT binding site was used to measure basal transcriptional activity. (c) The NFAT reporter construct was cotransfected with either a control construct ( SV40 T-Ag) or a construct expressing the SV40 large T-Ag ( SV40 T-Ag).
Seh, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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okt8  (ATCC)
90
ATCC okt8
FIG. 2. NFAT4 is functionally active in glial cells. (a) NFAT expression in SVG-A and U-87MG whole-cell lysates. NFATs 1 to 4 were detected using antibodies (NFATc1 7A6, NFATc2 4G6-G5, NFATc3 F-1, and NFATc4 H-74; <t>Santa</t> <t>Cruz</t> Biotech Inc.) diluted 1:200. <t>Ramos</t> cell lysates were used as a positive control. (b) Luciferase reporter gene assays using an NFAT-responsive reporter construct were used to compare NFAT activity in SVG-A cells and U-87MG cells in the presence or absence of 292 mg/liter glutamate. A control construct that lacked the NFAT binding site was used to measure basal transcriptional activity. (c) The NFAT reporter construct was cotransfected with either a control construct ( SV40 T-Ag) or a construct expressing the SV40 large T-Ag ( SV40 T-Ag).
Okt8, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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t pyogenes strain  (ATCC)
95
ATCC t pyogenes strain
FIG. 2. NFAT4 is functionally active in glial cells. (a) NFAT expression in SVG-A and U-87MG whole-cell lysates. NFATs 1 to 4 were detected using antibodies (NFATc1 7A6, NFATc2 4G6-G5, NFATc3 F-1, and NFATc4 H-74; <t>Santa</t> <t>Cruz</t> Biotech Inc.) diluted 1:200. <t>Ramos</t> cell lysates were used as a positive control. (b) Luciferase reporter gene assays using an NFAT-responsive reporter construct were used to compare NFAT activity in SVG-A cells and U-87MG cells in the presence or absence of 292 mg/liter glutamate. A control construct that lacked the NFAT binding site was used to measure basal transcriptional activity. (c) The NFAT reporter construct was cotransfected with either a control construct ( SV40 T-Ag) or a construct expressing the SV40 large T-Ag ( SV40 T-Ag).
T Pyogenes Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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huntoon 1923  (ATCC)
95
ATCC huntoon 1923
FIG. 2. NFAT4 is functionally active in glial cells. (a) NFAT expression in SVG-A and U-87MG whole-cell lysates. NFATs 1 to 4 were detected using antibodies (NFATc1 7A6, NFATc2 4G6-G5, NFATc3 F-1, and NFATc4 H-74; <t>Santa</t> <t>Cruz</t> Biotech Inc.) diluted 1:200. <t>Ramos</t> cell lysates were used as a positive control. (b) Luciferase reporter gene assays using an NFAT-responsive reporter construct were used to compare NFAT activity in SVG-A cells and U-87MG cells in the presence or absence of 292 mg/liter glutamate. A control construct that lacked the NFAT binding site was used to measure basal transcriptional activity. (c) The NFAT reporter construct was cotransfected with either a control construct ( SV40 T-Ag) or a construct expressing the SV40 large T-Ag ( SV40 T-Ag).
Huntoon 1923, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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smf condition s avermitilis nrrl 8165  (ATCC)
90
ATCC smf condition s avermitilis nrrl 8165
FIG. 2. NFAT4 is functionally active in glial cells. (a) NFAT expression in SVG-A and U-87MG whole-cell lysates. NFATs 1 to 4 were detected using antibodies (NFATc1 7A6, NFATc2 4G6-G5, NFATc3 F-1, and NFATc4 H-74; <t>Santa</t> <t>Cruz</t> Biotech Inc.) diluted 1:200. <t>Ramos</t> cell lysates were used as a positive control. (b) Luciferase reporter gene assays using an NFAT-responsive reporter construct were used to compare NFAT activity in SVG-A cells and U-87MG cells in the presence or absence of 292 mg/liter glutamate. A control construct that lacked the NFAT binding site was used to measure basal transcriptional activity. (c) The NFAT reporter construct was cotransfected with either a control construct ( SV40 T-Ag) or a construct expressing the SV40 large T-Ag ( SV40 T-Ag).
Smf Condition S Avermitilis Nrrl 8165, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nrrl b 33157  (ATCC)
93
ATCC nrrl b 33157
FIG. 2. NFAT4 is functionally active in glial cells. (a) NFAT expression in SVG-A and U-87MG whole-cell lysates. NFATs 1 to 4 were detected using antibodies (NFATc1 7A6, NFATc2 4G6-G5, NFATc3 F-1, and NFATc4 H-74; <t>Santa</t> <t>Cruz</t> Biotech Inc.) diluted 1:200. <t>Ramos</t> cell lysates were used as a positive control. (b) Luciferase reporter gene assays using an NFAT-responsive reporter construct were used to compare NFAT activity in SVG-A cells and U-87MG cells in the presence or absence of 292 mg/liter glutamate. A control construct that lacked the NFAT binding site was used to measure basal transcriptional activity. (c) The NFAT reporter construct was cotransfected with either a control construct ( SV40 T-Ag) or a construct expressing the SV40 large T-Ag ( SV40 T-Ag).
Nrrl B 33157, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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Image Search Results


Figure 2. Inhibition of NFATc1 in MC38Met cells. Effect of control RNAi (siScr) or NFATc1-specific RNAi on specific NFAT mRNA species: NFATc1 (A), NFATc2 (B), NFATc3 (C). D, the effect of NFATc1 siRNA versus scrambled control si-Scr on NFAT family protein levels in MC38Met cells, and steady state levels in both MC38Par and MC38Met cells with Ramos cell lysate positive control in the right hand lane. E, relative rates of invasion for cells shown in A–D . , P < 0.0005; , P < 0.00005.

Journal: Cancer Research

Article Title: Nuclear Factor of Activated T-cell Activity Is Associated with Metastatic Capacity in Colon Cancer

doi: 10.1158/0008-5472.can-14-1592

Figure Lengend Snippet: Figure 2. Inhibition of NFATc1 in MC38Met cells. Effect of control RNAi (siScr) or NFATc1-specific RNAi on specific NFAT mRNA species: NFATc1 (A), NFATc2 (B), NFATc3 (C). D, the effect of NFATc1 siRNA versus scrambled control si-Scr on NFAT family protein levels in MC38Met cells, and steady state levels in both MC38Par and MC38Met cells with Ramos cell lysate positive control in the right hand lane. E, relative rates of invasion for cells shown in A–D . , P < 0.0005; , P < 0.00005.

Article Snippet: Ramos cell (Burkitt lymphoma, B lymphocytes) lysate (Santa Cruz Biotechnology) was used as positive control.

Techniques: Inhibition, Control, Positive Control

Figure 7. Knockdown of NFATc1 in MC38Met cells decreases tumor incidence and liver metastases. A, Western blot analysis showing NFAT proteins in MC38MetþshCtrl and MC38MetþshNFATc1 cell lines. b-actin was used as a loading control and Ramos cell extract as positive control. B, analysis of NFATc1 specific mRNA in cells shown in A. C, rates of trans-endothelial invasion for cells are shown in A and B. Individual replicate wells from a representative experiment are plotted with the mean and the SEM (bars and whiskers). D, representative (n ¼ 14-15 mice/group) bioluminescence image corresponding day 21. E, summary- of bioluminescence signal quantified at day 21. F, summary incidence of liver metastases. G, liver metastases measured by liver weight to body weight ratio at 21 days postinjection. , P < 0.03; , P < 0.001; , P < 0.0001.

Journal: Cancer Research

Article Title: Nuclear Factor of Activated T-cell Activity Is Associated with Metastatic Capacity in Colon Cancer

doi: 10.1158/0008-5472.can-14-1592

Figure Lengend Snippet: Figure 7. Knockdown of NFATc1 in MC38Met cells decreases tumor incidence and liver metastases. A, Western blot analysis showing NFAT proteins in MC38MetþshCtrl and MC38MetþshNFATc1 cell lines. b-actin was used as a loading control and Ramos cell extract as positive control. B, analysis of NFATc1 specific mRNA in cells shown in A. C, rates of trans-endothelial invasion for cells are shown in A and B. Individual replicate wells from a representative experiment are plotted with the mean and the SEM (bars and whiskers). D, representative (n ¼ 14-15 mice/group) bioluminescence image corresponding day 21. E, summary- of bioluminescence signal quantified at day 21. F, summary incidence of liver metastases. G, liver metastases measured by liver weight to body weight ratio at 21 days postinjection. , P < 0.03; , P < 0.001; , P < 0.0001.

Article Snippet: Ramos cell (Burkitt lymphoma, B lymphocytes) lysate (Santa Cruz Biotechnology) was used as positive control.

Techniques: Knockdown, Western Blot, Control, Positive Control

FIG. 2. Immunoblot analysis of TLR proteins in male reproductive organs. Aliquots (100 lg) of cytoplasmic protein extracts were separated by PAGE, electroblotted, and blots probed with anti-TLR antibodies followed by enhanced chemiluminescence detection using Pierce Super- Signal West Pico or Pierce SuperSignal West Femto (*F) substrate. Representative results are shown (n ¼ 3–5 rats). þ, control rat tissue extracts from spleen, TLRs 1–7; lung, TLR8; and small intestine, TLR9. þ Ext, positive-control whole cell lysates used were Raw 264 Abelson transformed macrophages (TLRs 1, 2–6, and 8–10); SW480 colorectal adenocarcinoma (TLR2); Daudi cell extract (TLR7); Ramos cell lysate (TLR10) and mouse heart whole cell lysate (TLR11). Blots were stripped and reprobed with anti-actin monoclonal antibody to detect actin as a loading control. Representative results are shown (n ¼ 3–5 rats) for blots exposed to film for the same length of time when using equivalent chemiluminescent substrate.

Journal: Biology of reproduction

Article Title: Members of the Toll-like receptor family of innate immunity pattern-recognition receptors are abundant in the male rat reproductive tract.

doi: 10.1095/biolreprod.106.059410

Figure Lengend Snippet: FIG. 2. Immunoblot analysis of TLR proteins in male reproductive organs. Aliquots (100 lg) of cytoplasmic protein extracts were separated by PAGE, electroblotted, and blots probed with anti-TLR antibodies followed by enhanced chemiluminescence detection using Pierce Super- Signal West Pico or Pierce SuperSignal West Femto (*F) substrate. Representative results are shown (n ¼ 3–5 rats). þ, control rat tissue extracts from spleen, TLRs 1–7; lung, TLR8; and small intestine, TLR9. þ Ext, positive-control whole cell lysates used were Raw 264 Abelson transformed macrophages (TLRs 1, 2–6, and 8–10); SW480 colorectal adenocarcinoma (TLR2); Daudi cell extract (TLR7); Ramos cell lysate (TLR10) and mouse heart whole cell lysate (TLR11). Blots were stripped and reprobed with anti-actin monoclonal antibody to detect actin as a loading control. Representative results are shown (n ¼ 3–5 rats) for blots exposed to film for the same length of time when using equivalent chemiluminescent substrate.

Article Snippet: Mouse heart tissue lysate (40102) and Ramos cell lysate (40175) was from Imgenex.

Techniques: Western Blot, Control, Positive Control, Transformation Assay

FIG. 2. NFAT4 is functionally active in glial cells. (a) NFAT expression in SVG-A and U-87MG whole-cell lysates. NFATs 1 to 4 were detected using antibodies (NFATc1 7A6, NFATc2 4G6-G5, NFATc3 F-1, and NFATc4 H-74; Santa Cruz Biotech Inc.) diluted 1:200. Ramos cell lysates were used as a positive control. (b) Luciferase reporter gene assays using an NFAT-responsive reporter construct were used to compare NFAT activity in SVG-A cells and U-87MG cells in the presence or absence of 292 mg/liter glutamate. A control construct that lacked the NFAT binding site was used to measure basal transcriptional activity. (c) The NFAT reporter construct was cotransfected with either a control construct ( SV40 T-Ag) or a construct expressing the SV40 large T-Ag ( SV40 T-Ag).

Journal: Journal of Virology

Article Title: NFAT4 Is Required for JC Virus Infection of Glial Cells

doi: 10.1128/jvi.01456-06

Figure Lengend Snippet: FIG. 2. NFAT4 is functionally active in glial cells. (a) NFAT expression in SVG-A and U-87MG whole-cell lysates. NFATs 1 to 4 were detected using antibodies (NFATc1 7A6, NFATc2 4G6-G5, NFATc3 F-1, and NFATc4 H-74; Santa Cruz Biotech Inc.) diluted 1:200. Ramos cell lysates were used as a positive control. (b) Luciferase reporter gene assays using an NFAT-responsive reporter construct were used to compare NFAT activity in SVG-A cells and U-87MG cells in the presence or absence of 292 mg/liter glutamate. A control construct that lacked the NFAT binding site was used to measure basal transcriptional activity. (c) The NFAT reporter construct was cotransfected with either a control construct ( SV40 T-Ag) or a construct expressing the SV40 large T-Ag ( SV40 T-Ag).

Article Snippet: Ramos cell lysates were purchased from Santa Cruz Biotech Inc. Whole-cell extracts were separated on Tris-HCl-ready gels (Bio-Rad).

Techniques: Expressing, Positive Control, Luciferase, Construct, Activity Assay, Control, Binding Assay