rac1 pulldown assay Search Results


rac1 activation assay kit  (Cytoskeleton Inc)
95
Cytoskeleton Inc rac1 activation assay kit
FIGURE 7. RACK1-silencing significantly decreased VEGF-induced small-GTPase <t>Rac1</t> activation and membrane ruffling. A, AG1-G1-Neo and AG1-G1- Flt1 cells transfected with control siRNA (si-ctl) or RACK1 siRNAs (si1 and si2) were serum-starved and stimulated with VEGF (100 ng/ml) for 3 min. Cell lysate was subjected to a pull-down assay with GST-PAK-PBD (GST-fused GTPase-binding domain) agarose beads, followed by Western blotting with anti-Rac1 antibody. RACK1-silencing remarkably decreased VEGF-induced activation of Rac1. Cell lysates were immunoblotted with antibodies against Rac1, and -actin as a control. B, AG1-G1-Neo and AG1-G1-Flt1 cells transfected with control siRNA (si-ctl) or RACK1 siRNAs (si1 and si2) were serum-starved and stimulated with VEGF (100ng/ml)for5min.Cellswerefixed,permeabilized,andstainedwithrhodamine-phalloidin(F-actin).Cellmorphologywasobservedbyconfocalmicroscope, and representative images are presented (upper panels). Scale bar, 10 m. The numbers of cells exhibiting membrane ruffling were counted (lower panels). RACK1-silencing significantly inhibited VEGF-induced membrane ruffling.
Rac1 Activation Assay Kit, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rac1+pulldown+assay/Rac1+Pull-down+Activation+Assay+Biochem+Kit/10__1074_slash_jbc__m110__165605-83-11-15
Average 95 stars, based on 1 article reviews
rac1 activation assay kit - by Bioz Stars, 2026-10
95/100 stars
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gst pak beads  (Cytoskeleton Inc)
95
Cytoskeleton Inc gst pak beads
FIGURE 7. RACK1-silencing significantly decreased VEGF-induced small-GTPase <t>Rac1</t> activation and membrane ruffling. A, AG1-G1-Neo and AG1-G1- Flt1 cells transfected with control siRNA (si-ctl) or RACK1 siRNAs (si1 and si2) were serum-starved and stimulated with VEGF (100 ng/ml) for 3 min. Cell lysate was subjected to a pull-down assay with GST-PAK-PBD (GST-fused GTPase-binding domain) agarose beads, followed by Western blotting with anti-Rac1 antibody. RACK1-silencing remarkably decreased VEGF-induced activation of Rac1. Cell lysates were immunoblotted with antibodies against Rac1, and -actin as a control. B, AG1-G1-Neo and AG1-G1-Flt1 cells transfected with control siRNA (si-ctl) or RACK1 siRNAs (si1 and si2) were serum-starved and stimulated with VEGF (100ng/ml)for5min.Cellswerefixed,permeabilized,andstainedwithrhodamine-phalloidin(F-actin).Cellmorphologywasobservedbyconfocalmicroscope, and representative images are presented (upper panels). Scale bar, 10 m. The numbers of cells exhibiting membrane ruffling were counted (lower panels). RACK1-silencing significantly inhibited VEGF-induced membrane ruffling.
Gst Pak Beads, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rac1+pulldown+assay/PAK-PBD+beads+-+binds+active+Rac+Cdc42+proteins/pm25344477-75-11-13
Average 95 stars, based on 1 article reviews
gst pak beads - by Bioz Stars, 2026-10
95/100 stars
  Buy from Supplier
rhoa pull down activation assay kit  (Cytoskeleton Inc)
95
Cytoskeleton Inc rhoa pull down activation assay kit
A) Effects of CD148 in E-cadherin contact formation were examined by a calcium-switch assay and immunofluorescence staining. CD148 WT, but not CS, promotes E-cadherin contact formation in A431D/E-cadherin WT cells, as evidenced by the expanded and intense E-cadherin distribution (left panels), while CD148 WT shows no effects in A431D/E-cadherin 764 AAA cells (right panels). B) Activities of Rac1, Cdc42, and <t>RhoA</t> were measured in the condition of calcium switch assay. Active and total levels of Rac1, Cdc42, and RhoA proteins were assessed by the pull-down assays and/or immunoblotting described in the “ ” (left panels). The activity was normalized to total amount of protein using densitometry and expressed as fold change (right panels). The data show means ± SEM of quadruplicate determinations. **P<0.05 vs. CD148 (−) cells.
Rhoa Pull Down Activation Assay Kit, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rac1+pulldown+assay/RhoA+Rac1+Cdc42+Activation+Assay+Combo+Biochem+Kit/pmc04227875-73-11-16
Average 95 stars, based on 1 article reviews
rhoa pull down activation assay kit - by Bioz Stars, 2026-10
95/100 stars
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Image Search Results


FIGURE 7. RACK1-silencing significantly decreased VEGF-induced small-GTPase Rac1 activation and membrane ruffling. A, AG1-G1-Neo and AG1-G1- Flt1 cells transfected with control siRNA (si-ctl) or RACK1 siRNAs (si1 and si2) were serum-starved and stimulated with VEGF (100 ng/ml) for 3 min. Cell lysate was subjected to a pull-down assay with GST-PAK-PBD (GST-fused GTPase-binding domain) agarose beads, followed by Western blotting with anti-Rac1 antibody. RACK1-silencing remarkably decreased VEGF-induced activation of Rac1. Cell lysates were immunoblotted with antibodies against Rac1, and -actin as a control. B, AG1-G1-Neo and AG1-G1-Flt1 cells transfected with control siRNA (si-ctl) or RACK1 siRNAs (si1 and si2) were serum-starved and stimulated with VEGF (100ng/ml)for5min.Cellswerefixed,permeabilized,andstainedwithrhodamine-phalloidin(F-actin).Cellmorphologywasobservedbyconfocalmicroscope, and representative images are presented (upper panels). Scale bar, 10 m. The numbers of cells exhibiting membrane ruffling were counted (lower panels). RACK1-silencing significantly inhibited VEGF-induced membrane ruffling.

Journal: Journal of Biological Chemistry

Article Title: RACK1 Regulates VEGF/Flt1-mediated Cell Migration via Activation of a PI3K/Akt Pathway

doi: 10.1074/jbc.m110.165605

Figure Lengend Snippet: FIGURE 7. RACK1-silencing significantly decreased VEGF-induced small-GTPase Rac1 activation and membrane ruffling. A, AG1-G1-Neo and AG1-G1- Flt1 cells transfected with control siRNA (si-ctl) or RACK1 siRNAs (si1 and si2) were serum-starved and stimulated with VEGF (100 ng/ml) for 3 min. Cell lysate was subjected to a pull-down assay with GST-PAK-PBD (GST-fused GTPase-binding domain) agarose beads, followed by Western blotting with anti-Rac1 antibody. RACK1-silencing remarkably decreased VEGF-induced activation of Rac1. Cell lysates were immunoblotted with antibodies against Rac1, and -actin as a control. B, AG1-G1-Neo and AG1-G1-Flt1 cells transfected with control siRNA (si-ctl) or RACK1 siRNAs (si1 and si2) were serum-starved and stimulated with VEGF (100ng/ml)for5min.Cellswerefixed,permeabilized,andstainedwithrhodamine-phalloidin(F-actin).Cellmorphologywasobservedbyconfocalmicroscope, and representative images are presented (upper panels). Scale bar, 10 m. The numbers of cells exhibiting membrane ruffling were counted (lower panels). RACK1-silencing significantly inhibited VEGF-induced membrane ruffling.

Article Snippet: Rac1 Activation Assay—The activation of Rac1 was analyzed by using a Rac1 activation assay kit (Cytoskeleton, Denver, CO) according to the instructions recommended by the manufacturer.

Techniques: Activation Assay, Membrane, Transfection, Control, Pull Down Assay, Binding Assay, Western Blot

FIGURE 8. The possible role of RACK1 in VEGF/Flt1-induced cell migra- tion. By interacting with Flt1, RACK1 plays an important role in VEGF/Flt1- induced cell migration by regulating the activation of the PI3K/Akt but not PLC-MAPK signaling pathway. RACK1 regulates the VEGF/Flt1-induced acti- vation of Rac1 and cytoskeletal dynamics.

Journal: Journal of Biological Chemistry

Article Title: RACK1 Regulates VEGF/Flt1-mediated Cell Migration via Activation of a PI3K/Akt Pathway

doi: 10.1074/jbc.m110.165605

Figure Lengend Snippet: FIGURE 8. The possible role of RACK1 in VEGF/Flt1-induced cell migra- tion. By interacting with Flt1, RACK1 plays an important role in VEGF/Flt1- induced cell migration by regulating the activation of the PI3K/Akt but not PLC-MAPK signaling pathway. RACK1 regulates the VEGF/Flt1-induced acti- vation of Rac1 and cytoskeletal dynamics.

Article Snippet: Rac1 Activation Assay—The activation of Rac1 was analyzed by using a Rac1 activation assay kit (Cytoskeleton, Denver, CO) according to the instructions recommended by the manufacturer.

Techniques: Migration, Activation Assay

A) Effects of CD148 in E-cadherin contact formation were examined by a calcium-switch assay and immunofluorescence staining. CD148 WT, but not CS, promotes E-cadherin contact formation in A431D/E-cadherin WT cells, as evidenced by the expanded and intense E-cadherin distribution (left panels), while CD148 WT shows no effects in A431D/E-cadherin 764 AAA cells (right panels). B) Activities of Rac1, Cdc42, and RhoA were measured in the condition of calcium switch assay. Active and total levels of Rac1, Cdc42, and RhoA proteins were assessed by the pull-down assays and/or immunoblotting described in the “ ” (left panels). The activity was normalized to total amount of protein using densitometry and expressed as fold change (right panels). The data show means ± SEM of quadruplicate determinations. **P<0.05 vs. CD148 (−) cells.

Journal: PLoS ONE

Article Title: CD148 Tyrosine Phosphatase Promotes Cadherin Cell Adhesion

doi: 10.1371/journal.pone.0112753

Figure Lengend Snippet: A) Effects of CD148 in E-cadherin contact formation were examined by a calcium-switch assay and immunofluorescence staining. CD148 WT, but not CS, promotes E-cadherin contact formation in A431D/E-cadherin WT cells, as evidenced by the expanded and intense E-cadherin distribution (left panels), while CD148 WT shows no effects in A431D/E-cadherin 764 AAA cells (right panels). B) Activities of Rac1, Cdc42, and RhoA were measured in the condition of calcium switch assay. Active and total levels of Rac1, Cdc42, and RhoA proteins were assessed by the pull-down assays and/or immunoblotting described in the “ ” (left panels). The activity was normalized to total amount of protein using densitometry and expressed as fold change (right panels). The data show means ± SEM of quadruplicate determinations. **P<0.05 vs. CD148 (−) cells.

Article Snippet: RhoA, Rac1, and Cdc42 activities were assessed using the Rac1 and RhoA pull-down activation assay kit (Cytoskeleton, Inc., Denver, CO) according to the manufacturer’s instruction.

Techniques: Immunofluorescence, Staining, Western Blot, Activity Assay

A and B) CD148-introduced or CD148-negative A431D/E-cadherin WT (panel A) and A431D/E-cadherin 764 AAA (panel B) cells were subjected to a hanging drop assay. Rac1, Cdc42, and RhoA activities were assessed at the indicated time points. Active and total levels of Rac1, Cdc42, and RhoA proteins were assessed by pull-down assays and/or immunoblot analysis (left panels). The relative levels of active versus total Rac1, Cdc42, and RhoA were quantified by densitometric analysis (right panels). The data show means ± SEM of quadruplicate determinations. **P<0.05 vs. CD148 (−) cells. CD148 WT, but not CS, increases Rac1 activity in A431D/E-cadherin WT cells, while this effect is not observed in A431D/E-cadherin 764 AAA cells. C) Effects of CD148 WT in cell-cell adhesion were assessed by a hanging drop assay in the presence or absence of Rac1 inhibitor NSC 23766 (75 µM). CD148 increase of cell-cell adhesion is largely diminished by Rac1 inhibition.

Journal: PLoS ONE

Article Title: CD148 Tyrosine Phosphatase Promotes Cadherin Cell Adhesion

doi: 10.1371/journal.pone.0112753

Figure Lengend Snippet: A and B) CD148-introduced or CD148-negative A431D/E-cadherin WT (panel A) and A431D/E-cadherin 764 AAA (panel B) cells were subjected to a hanging drop assay. Rac1, Cdc42, and RhoA activities were assessed at the indicated time points. Active and total levels of Rac1, Cdc42, and RhoA proteins were assessed by pull-down assays and/or immunoblot analysis (left panels). The relative levels of active versus total Rac1, Cdc42, and RhoA were quantified by densitometric analysis (right panels). The data show means ± SEM of quadruplicate determinations. **P<0.05 vs. CD148 (−) cells. CD148 WT, but not CS, increases Rac1 activity in A431D/E-cadherin WT cells, while this effect is not observed in A431D/E-cadherin 764 AAA cells. C) Effects of CD148 WT in cell-cell adhesion were assessed by a hanging drop assay in the presence or absence of Rac1 inhibitor NSC 23766 (75 µM). CD148 increase of cell-cell adhesion is largely diminished by Rac1 inhibition.

Article Snippet: RhoA, Rac1, and Cdc42 activities were assessed using the Rac1 and RhoA pull-down activation assay kit (Cytoskeleton, Inc., Denver, CO) according to the manufacturer’s instruction.

Techniques: Western Blot, Activity Assay, Inhibition