rabbit polyclonal p cdc25c Search Results


rabbit polyclonal p cdc25c  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc rabbit polyclonal p cdc25c
Rabbit Polyclonal P Cdc25c, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit polyclonal p cdc25c/product/Cell Signaling Technology Inc
Average 95 stars, based on 1 article reviews
rabbit polyclonal p cdc25c - by Bioz Stars, 2026-02
95/100 stars
  Buy from Supplier
rabbit monoclonal anti-flag  (Millipore)
90
Millipore rabbit monoclonal anti-flag
(A) The layout shows how the experiment was performed. IMR-90 cells were synchronized with 2 mM thymidine and transfected with DN-RSK or empty vector as described in the Materials and Methods. Cells were treated with 5 μM CD or only DMSO as a control at 5.5–6 h after the second release and collected at each indicated time point after the second release. Each cell lysate was resolved by 8% SDS-PAGE and blotted with (B) <t>anti-FLAG</t> and anti-actin, (C) p-ERK1/2 and p-Cdc2 (Tyr 15), (D) p-Cdc25C (Ser 216), and p-Wee1 (Ser 642). Each membrane was reprobed with anti-actin as a loading control.
Rabbit Monoclonal Anti Flag, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit monoclonal anti-flag/product/Millipore
Average 90 stars, based on 1 article reviews
rabbit monoclonal anti-flag - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
rabbit polyclonal p erk1 2  (Cell Signaling Technology Inc)
99
Cell Signaling Technology Inc rabbit polyclonal p erk1 2
(A) The layout shows how the experiment was performed. IMR-90 cells were synchronized with 2 mM thymidine and transfected with DN-RSK or empty vector as described in the Materials and Methods. Cells were treated with 5 μM CD or only DMSO as a control at 5.5–6 h after the second release and collected at each indicated time point after the second release. Each cell lysate was resolved by 8% SDS-PAGE and blotted with (B) <t>anti-FLAG</t> and anti-actin, (C) p-ERK1/2 and p-Cdc2 (Tyr 15), (D) p-Cdc25C (Ser 216), and p-Wee1 (Ser 642). Each membrane was reprobed with anti-actin as a loading control.
Rabbit Polyclonal P Erk1 2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit polyclonal p erk1 2/product/Cell Signaling Technology Inc
Average 99 stars, based on 1 article reviews
rabbit polyclonal p erk1 2 - by Bioz Stars, 2026-02
99/100 stars
  Buy from Supplier
rabbit monoclonal p wee1  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc rabbit monoclonal p wee1
(A) The layout shows how the experiment was performed. IMR-90 cells were synchronized with 2 mM thymidine and transfected with DN-RSK or empty vector as described in the Materials and Methods. Cells were treated with 5 μM CD or only DMSO as a control at 5.5–6 h after the second release and collected at each indicated time point after the second release. Each cell lysate was resolved by 8% SDS-PAGE and blotted with (B) <t>anti-FLAG</t> and anti-actin, (C) p-ERK1/2 and p-Cdc2 (Tyr 15), (D) p-Cdc25C (Ser 216), and p-Wee1 (Ser 642). Each membrane was reprobed with anti-actin as a loading control.
Rabbit Monoclonal P Wee1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit monoclonal p wee1/product/Cell Signaling Technology Inc
Average 95 stars, based on 1 article reviews
rabbit monoclonal p wee1 - by Bioz Stars, 2026-02
95/100 stars
  Buy from Supplier
rabbit polyclonal anti wee1  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc rabbit polyclonal anti wee1
(A) The layout shows how the experiment was performed. IMR-90 cells were synchronized with 2 mM thymidine and transfected with DN-RSK or empty vector as described in the Materials and Methods. Cells were treated with 5 μM CD or only DMSO as a control at 5.5–6 h after the second release and collected at each indicated time point after the second release. Each cell lysate was resolved by 8% SDS-PAGE and blotted with (B) <t>anti-FLAG</t> and anti-actin, (C) p-ERK1/2 and p-Cdc2 (Tyr 15), (D) p-Cdc25C (Ser 216), and p-Wee1 (Ser 642). Each membrane was reprobed with anti-actin as a loading control.
Rabbit Polyclonal Anti Wee1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit polyclonal anti wee1/product/Cell Signaling Technology Inc
Average 95 stars, based on 1 article reviews
rabbit polyclonal anti wee1 - by Bioz Stars, 2026-02
95/100 stars
  Buy from Supplier
rabbit polyclonal anti β actin  (Cell Signaling Technology Inc)
99
Cell Signaling Technology Inc rabbit polyclonal anti β actin
(A) The layout shows how the experiment was performed. IMR-90 cells were synchronized with 2 mM thymidine and transfected with DN-RSK or empty vector as described in the Materials and Methods. Cells were treated with 5 μM CD or only DMSO as a control at 5.5–6 h after the second release and collected at each indicated time point after the second release. Each cell lysate was resolved by 8% SDS-PAGE and blotted with (B) <t>anti-FLAG</t> and anti-actin, (C) p-ERK1/2 and p-Cdc2 (Tyr 15), (D) p-Cdc25C (Ser 216), and p-Wee1 (Ser 642). Each membrane was reprobed with anti-actin as a loading control.
Rabbit Polyclonal Anti β Actin, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit polyclonal anti β actin/product/Cell Signaling Technology Inc
Average 99 stars, based on 1 article reviews
rabbit polyclonal anti β actin - by Bioz Stars, 2026-02
99/100 stars
  Buy from Supplier
rabbit polyclonal anti rsk1  (Cell Signaling Technology Inc)
94
Cell Signaling Technology Inc rabbit polyclonal anti rsk1
(A) The layout shows how the experiment was performed. IMR-90 cells were synchronized with 2 mM thymidine and transfected with DN-RSK or empty vector as described in the Materials and Methods. Cells were treated with 5 μM CD or only DMSO as a control at 5.5–6 h after the second release and collected at each indicated time point after the second release. Each cell lysate was resolved by 8% SDS-PAGE and blotted with (B) <t>anti-FLAG</t> and anti-actin, (C) p-ERK1/2 and p-Cdc2 (Tyr 15), (D) p-Cdc25C (Ser 216), and p-Wee1 (Ser 642). Each membrane was reprobed with anti-actin as a loading control.
Rabbit Polyclonal Anti Rsk1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit polyclonal anti rsk1/product/Cell Signaling Technology Inc
Average 94 stars, based on 1 article reviews
rabbit polyclonal anti rsk1 - by Bioz Stars, 2026-02
94/100 stars
  Buy from Supplier
mouse monoclonal anti cyclin a  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc mouse monoclonal anti cyclin a
(A) The layout shows how the experiment was performed. IMR-90 cells were synchronized with 2 mM thymidine and transfected with DN-RSK or empty vector as described in the Materials and Methods. Cells were treated with 5 μM CD or only DMSO as a control at 5.5–6 h after the second release and collected at each indicated time point after the second release. Each cell lysate was resolved by 8% SDS-PAGE and blotted with (B) <t>anti-FLAG</t> and anti-actin, (C) p-ERK1/2 and p-Cdc2 (Tyr 15), (D) p-Cdc25C (Ser 216), and p-Wee1 (Ser 642). Each membrane was reprobed with anti-actin as a loading control.
Mouse Monoclonal Anti Cyclin A, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse monoclonal anti cyclin a/product/Cell Signaling Technology Inc
Average 96 stars, based on 1 article reviews
mouse monoclonal anti cyclin a - by Bioz Stars, 2026-02
96/100 stars
  Buy from Supplier
mouse monoclonal p h3  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc mouse monoclonal p h3
(A) The layout shows how the experiment was performed. IMR-90 cells were synchronized with 2 mM thymidine and transfected with DN-RSK or empty vector as described in the Materials and Methods. Cells were treated with 5 μM CD or only DMSO as a control at 5.5–6 h after the second release and collected at each indicated time point after the second release. Each cell lysate was resolved by 8% SDS-PAGE and blotted with (B) <t>anti-FLAG</t> and anti-actin, (C) p-ERK1/2 and p-Cdc2 (Tyr 15), (D) p-Cdc25C (Ser 216), and p-Wee1 (Ser 642). Each membrane was reprobed with anti-actin as a loading control.
Mouse Monoclonal P H3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse monoclonal p h3/product/Cell Signaling Technology Inc
Average 96 stars, based on 1 article reviews
mouse monoclonal p h3 - by Bioz Stars, 2026-02
96/100 stars
  Buy from Supplier
cdc25c  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc cdc25c
(A) The layout shows how the experiment was performed. IMR-90 cells were synchronized with 2 mM thymidine and transfected with DN-RSK or empty vector as described in the Materials and Methods. Cells were treated with 5 μM CD or only DMSO as a control at 5.5–6 h after the second release and collected at each indicated time point after the second release. Each cell lysate was resolved by 8% SDS-PAGE and blotted with (B) <t>anti-FLAG</t> and anti-actin, (C) p-ERK1/2 and p-Cdc2 (Tyr 15), (D) p-Cdc25C (Ser 216), and p-Wee1 (Ser 642). Each membrane was reprobed with anti-actin as a loading control.
Cdc25c, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cdc25c/product/Cell Signaling Technology Inc
Average 95 stars, based on 1 article reviews
cdc25c - by Bioz Stars, 2026-02
95/100 stars
  Buy from Supplier
rabbit polyclonal anti erk1 2  (Cell Signaling Technology Inc)
99
Cell Signaling Technology Inc rabbit polyclonal anti erk1 2
(A) The layout shows how the experiment was performed. IMR-90 cells were synchronized with 2 mM thymidine and transfected with DN-RSK or empty vector as described in the Materials and Methods. Cells were treated with 5 μM CD or only DMSO as a control at 5.5–6 h after the second release and collected at each indicated time point after the second release. Each cell lysate was resolved by 8% SDS-PAGE and blotted with (B) <t>anti-FLAG</t> and anti-actin, (C) p-ERK1/2 and p-Cdc2 (Tyr 15), (D) p-Cdc25C (Ser 216), and p-Wee1 (Ser 642). Each membrane was reprobed with anti-actin as a loading control.
Rabbit Polyclonal Anti Erk1 2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit polyclonal anti erk1 2/product/Cell Signaling Technology Inc
Average 99 stars, based on 1 article reviews
rabbit polyclonal anti erk1 2 - by Bioz Stars, 2026-02
99/100 stars
  Buy from Supplier
rabbit monoclonal histone h3  (Cell Signaling Technology Inc)
99
Cell Signaling Technology Inc rabbit monoclonal histone h3
(A) The layout shows how the experiment was performed. IMR-90 cells were synchronized with 2 mM thymidine and transfected with DN-RSK or empty vector as described in the Materials and Methods. Cells were treated with 5 μM CD or only DMSO as a control at 5.5–6 h after the second release and collected at each indicated time point after the second release. Each cell lysate was resolved by 8% SDS-PAGE and blotted with (B) <t>anti-FLAG</t> and anti-actin, (C) p-ERK1/2 and p-Cdc2 (Tyr 15), (D) p-Cdc25C (Ser 216), and p-Wee1 (Ser 642). Each membrane was reprobed with anti-actin as a loading control.
Rabbit Monoclonal Histone H3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit monoclonal histone h3/product/Cell Signaling Technology Inc
Average 99 stars, based on 1 article reviews
rabbit monoclonal histone h3 - by Bioz Stars, 2026-02
99/100 stars
  Buy from Supplier

Image Search Results


(A) The layout shows how the experiment was performed. IMR-90 cells were synchronized with 2 mM thymidine and transfected with DN-RSK or empty vector as described in the Materials and Methods. Cells were treated with 5 μM CD or only DMSO as a control at 5.5–6 h after the second release and collected at each indicated time point after the second release. Each cell lysate was resolved by 8% SDS-PAGE and blotted with (B) anti-FLAG and anti-actin, (C) p-ERK1/2 and p-Cdc2 (Tyr 15), (D) p-Cdc25C (Ser 216), and p-Wee1 (Ser 642). Each membrane was reprobed with anti-actin as a loading control.

Journal: Molecules and Cells

Article Title: Actin Dysfunction Induces Cell Cycle Delay at G2/M with Sustained ERK and RSK Activation in IMR-90 Normal Human Fibroblasts

doi: 10.14348/molcells.2018.2266

Figure Lengend Snippet: (A) The layout shows how the experiment was performed. IMR-90 cells were synchronized with 2 mM thymidine and transfected with DN-RSK or empty vector as described in the Materials and Methods. Cells were treated with 5 μM CD or only DMSO as a control at 5.5–6 h after the second release and collected at each indicated time point after the second release. Each cell lysate was resolved by 8% SDS-PAGE and blotted with (B) anti-FLAG and anti-actin, (C) p-ERK1/2 and p-Cdc2 (Tyr 15), (D) p-Cdc25C (Ser 216), and p-Wee1 (Ser 642). Each membrane was reprobed with anti-actin as a loading control.

Article Snippet: Rabbit polyclonal p-Cdc2 (Tyr 15) (ab47594, Abcam, USA), mouse monoclonal anti-cyclin A (4656, Cell Signaling Technology, USA), rabbit polyclonal p-Cdc25C (Ser 216) (9528, Cell Signaling Technology), rabbit monoclonal anti-Cdc25C (ab32444, Abcam), rabbit polyclonal anti-β-actin (4967, Cell Signaling Technology), rabbit monoclonal p-Wee1 (Ser 642) (4910, Cell Signaling Technology), rabbit polyclonal anti-Wee1 (4936, Cell Signaling Technology), rabbit monoclonal anti-FLAG (F7425, Sigma), rabbit polyclonal p-ERK1/2 (Thr 202/Tyr 204) (9101, Cell Signaling), rabbit polyclonal anti-ERK1/2 (9102, Cell Signaling), rabbit monoclonal p-RSK1 (Ser 380) (ab32203, Abcam), rabbit polyclonal anti-RSK1 (9333, Cell Signaling), rabbit monoclonal histone H3 (4499, Cell Signaling Technology), and mouse monoclonal p-H3 (Ser 10) (9706, Cell Signaling Technology) were used in this study.

Techniques: Transfection, Plasmid Preparation, SDS Page