Journal: British Journal of Pharmacology

Article Title: Activation of P2Y 6 receptors increases the voiding frequency in anaesthetized rats by releasing ATP from the bladder urothelium

doi: 10.1111/bph.12711

Figure Lengend Snippet: Primary and secondary antibodies used in immunohistochemistry experiments

Article Snippet: Observations were performed and analysed with a laser-scanning confocal microscope (Olympus FluoView, FV1000, Tokyo, Japan). table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Antigen Code Host Dilution Supplier Primary antibodies P2Y 1 APR-009 Rabbit 1:75 Alomone P2Y 6 APR-011 Rabbit 1:100 Alomone P2Y 6 ABIN1386282 Rabbit 1:150 Antibodies Online P2X1 APR-001 Rabbit 1:50 Alomone P2X3 APR-016 Rabbit 1:100 Alomone NTPDase2 BZ1-5F Rabbit 1:400 J. Sevigny Cytokeratin 20 (CK20) M7019 Mouse 1:100 Dako Vimentin M0725 Mouse 1:150 Dako NF160 Ab7794 Mouse 1:600 Abcam VAChT AB1578 Goat 1:750 Chemicon Secondary antibodies Alexa Fluor 488 anti-rb A-21206 Donkey 1:1500 Mol.

Techniques: Immunohistochemistry

Journal: British Journal of Pharmacology

Article Title: Activation of P2Y 6 receptors increases the voiding frequency in anaesthetized rats by releasing ATP from the bladder urothelium

doi: 10.1111/bph.12711

Figure Lengend Snippet: (A) Setup for myographic recordings of the whole urinary bladder of the rat in vitro. (B) Spontaneous contractile activity of the rat urinary bladder in response to bladder filling with Tyrode's solution, up to 150 μL, infused at a constant flow rate (40 μL·min−1) to mimic in vivo cystometry experiments. UDP (300 μM) was superfused either into the bladder lumen (by changing the syringe connected to the automated perfusion system) or directly to the bathing solution outside the bladder. (C) Quantification of the frequency and magnitude of spontaneous contractions of the whole bladder in vitro in the absence and in the presence of UDP (300 μM) applied inside and outside the bladder. The vertical bars represent SEM of four isolated bladders. *P < 0.05; significantly different from control (saline superfusion); one-way anova followed by Dunnett's modified t-test. (D) Confocal micrographs of transverse sections of rat urinary bladder detrusor muscle immunostained for P2Y6 (APR-011) and P2X1 (APR-001) receptors. Scale bars = 50 μm.

Article Snippet: Observations were performed and analysed with a laser-scanning confocal microscope (Olympus FluoView, FV1000, Tokyo, Japan). table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Antigen Code Host Dilution Supplier Primary antibodies P2Y 1 APR-009 Rabbit 1:75 Alomone P2Y 6 APR-011 Rabbit 1:100 Alomone P2Y 6 ABIN1386282 Rabbit 1:150 Antibodies Online P2X1 APR-001 Rabbit 1:50 Alomone P2X3 APR-016 Rabbit 1:100 Alomone NTPDase2 BZ1-5F Rabbit 1:400 J. Sevigny Cytokeratin 20 (CK20) M7019 Mouse 1:100 Dako Vimentin M0725 Mouse 1:150 Dako NF160 Ab7794 Mouse 1:600 Abcam VAChT AB1578 Goat 1:750 Chemicon Secondary antibodies Alexa Fluor 488 anti-rb A-21206 Donkey 1:1500 Mol.

Techniques: In Vitro, Activity Assay, In Vivo, Isolation, Modification