Journal: Neural Plasticity

Article Title: Transplantation of Neural Stem Cells Cotreated with Thyroid Hormone and GDNF Gene Induces Neuroprotection in Rats of Chronic Experimental Allergic Encephalomyelitis

doi: 10.1155/2016/3081939

Figure Lengend Snippet: Immunohistochemical double staining of BrdU, MAP2, GFAP, and GalC positive cells in the cerebral parenchyma of rats. (a–c) MAP2 − /BrdU + cells (solid arrow), MAP2 + /BrdU − cells (dotted arrow), and MAP2 + /BrdU + cells (arrowhead) in NSCs, T3/NSCs, and GDNF-T3/NSCs group, respectively. (d–f) GalC − /BrdU + positive cells (solid arrow), GalC + /BrdU − cells (dotted arrow), and GalC + /BrdU + cells (arrowhead) in NSCs, T3/NSCs, and GDNF-T3/NSCs group, respectively. (g–i) GFAP − /BrdU + cells (solid arrow), GFAP + /BrdU − cells (dotted arrow), and GFAP + /BrdU + cells (arrowhead) in NSCs, T3/NSCs, and GDNF-T3/NSCs group, respectively. (j) GFAP + cells (dotted arrow) in control group. (k) GFAP + cells (dotted arrow) in normal group. Bar (a–k) = 25 μ m.

Article Snippet: After a goat anti-mouse IgG secondary antibody (1 : 100; Wuhan Boster Biological Technology, China) was added for 30 minutes, alkaline phosphatase- (AP-) streptavidin (Boster) was incubated for 30 minutes, and 5-bromo-4-chloro-3-indolyl phosphate/nitroblue tetrazolium chloride (BCIP/NBT, Boster) was then used as a chromogen for 10 min. After PBS washes, the sections were reincubated with primary antibody for rabbit ant-MAP2, GFAP, or GalC, followed by incubation with goat anti-rabbit IgG (1 : 100; Boster) and horseradish peroxidase- (HRP-) streptavidin (Boster).

Techniques: Immunohistochemical staining, Double Staining, Control

Journal: Neural Plasticity

Article Title: Transplantation of Neural Stem Cells Cotreated with Thyroid Hormone and GDNF Gene Induces Neuroprotection in Rats of Chronic Experimental Allergic Encephalomyelitis

doi: 10.1155/2016/3081939

Figure Lengend Snippet: Differentiation of NSCs, T3/NSCs, and GDNF-T3/NSCs in vivo (mean ± SD, n = 5).

Article Snippet: After a goat anti-mouse IgG secondary antibody (1 : 100; Wuhan Boster Biological Technology, China) was added for 30 minutes, alkaline phosphatase- (AP-) streptavidin (Boster) was incubated for 30 minutes, and 5-bromo-4-chloro-3-indolyl phosphate/nitroblue tetrazolium chloride (BCIP/NBT, Boster) was then used as a chromogen for 10 min. After PBS washes, the sections were reincubated with primary antibody for rabbit ant-MAP2, GFAP, or GalC, followed by incubation with goat anti-rabbit IgG (1 : 100; Boster) and horseradish peroxidase- (HRP-) streptavidin (Boster).

Techniques: In Vivo

Journal: Cell

Article Title: Neuromodulator Signaling Bidirectionally Controls Vesicle Numbers in Human Synapses

doi: 10.1016/j.cell.2019.09.011

Figure Lengend Snippet: A Human neurons (1 month old) before and after acute treatment with norepinephrine stained for presynaptic markers Syph, synapsin (‘Pan-Syn’) and MAP2.

Article Snippet: Chicken polyclonal ant MAP2 , Encor , CPCA-MAP2.

Techniques: Staining

Journal: Cell

Article Title: Neuromodulator Signaling Bidirectionally Controls Vesicle Numbers in Human Synapses

doi: 10.1016/j.cell.2019.09.011

Figure Lengend Snippet: A, B and C Puncta densities of acutely forskolin treated (10 µM, 30 mins) and control and Syn1-deficient human neurons stained for pan-Synapsin (A), Syt1 (A), Syph (B), Homer (C) and MAP2. Puncta areas are depicted in Figure S4.

Article Snippet: Chicken polyclonal ant MAP2 , Encor , CPCA-MAP2.

Techniques: Control, Staining

Journal: Cell

Article Title: Neuromodulator Signaling Bidirectionally Controls Vesicle Numbers in Human Synapses

doi: 10.1016/j.cell.2019.09.011

Figure Lengend Snippet: Highlights:

Article Snippet: Chicken polyclonal ant MAP2 , Encor , CPCA-MAP2.

Techniques: Transduction, Recombinant, Suspension, Plasmid Preparation, Expressing, Software

Journal: Cell Death & Disease

Article Title: MAG induces apoptosis in cerebellar granule neurons through p75 NTR demarcating granule layer/white matter boundary

doi: 10.1038/s41419-019-1970-x

Figure Lengend Snippet: a Representative images of P2, P4, P7, P10, P14 and P60 wild type whole cerebellar sections triple-labelled by immunoflorescence with anti-MAG, anti-NgR1 and anti-p75 NTR antibodies and counterstained with DAPI. Scale bar, 1 mm. b , c Representative confocal images of P2, P4, P7, P10 and P14 wild-type IGL of folium III triple-labelled by immunoflourescence with anti-MAG, anti-NgR1, anti-p75 NTR and DAPI. Scale bar for low magnification images b is 50 μm;scale bar inserts c , 25 μm. Abbreviations: WM, white matter; IGL, internal granule layer

Article Snippet: The primary antibodies used in this study were: polyclonal anti-p75 NTR (Neuromics, GT15057, 1:200), monoclonal anti-MAG (Millipore, Mab1567, 1:200), polyclonal anti-Nogo receptor (NgR1, Alomone, ANT-008, 1:200), polyclonal anti-cleaved caspase 3 (Cell signal, 9761, 1:400), monoclonal anti-NeuN (Millipore, MAB377, 1:200), monoclonal anti-GFAP (Abcam, 1:500), polyclonal anti-Iba1 (Abcam, Ab5076, 1:200), monoclonal anti–β-III tubulin (R&D systems, MAB1195, 1:10000), polyclonal anti-MAP2 (Abcam, ab5392, 1:2000) and polyclonal anti-GFP (Abcam, ab13970, 1:500).

Techniques:

Journal: Cell Death & Disease

Article Title: MAG induces apoptosis in cerebellar granule neurons through p75 NTR demarcating granule layer/white matter boundary

doi: 10.1038/s41419-019-1970-x

Figure Lengend Snippet: a–b Micrographs of representative P7 CGNs double-labelled with anti-MAG ( a ), anti-NgR1 or anti-p75 NTR ( b ) combined with anti- β III tubulin and counterstained with DAPI after 24 h in culture. Scale bars, 20 μm. c–e NgR1 mRNA ( c ), NgR2 mRNA ( d ) and p75 NTR mRNA ( e ) levels relative to the reference mRNA of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) in P7 CGNs cultured for 1, 2 and 3 days in vitro. Mean ± s.e.m of data from eight separate cultures is shown

Article Snippet: The primary antibodies used in this study were: polyclonal anti-p75 NTR (Neuromics, GT15057, 1:200), monoclonal anti-MAG (Millipore, Mab1567, 1:200), polyclonal anti-Nogo receptor (NgR1, Alomone, ANT-008, 1:200), polyclonal anti-cleaved caspase 3 (Cell signal, 9761, 1:400), monoclonal anti-NeuN (Millipore, MAB377, 1:200), monoclonal anti-GFAP (Abcam, 1:500), polyclonal anti-Iba1 (Abcam, Ab5076, 1:200), monoclonal anti–β-III tubulin (R&D systems, MAB1195, 1:10000), polyclonal anti-MAP2 (Abcam, ab5392, 1:2000) and polyclonal anti-GFP (Abcam, ab13970, 1:500).

Techniques: Cell Culture, In Vitro