rab4 Search Results


90
Bioss rab4 22 bioss bs 6157r if
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novus biologicals nbp2-37485
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Cell Signaling Technology Inc rab4 rabbit cell signaling technologies ab 225357 9 2167s if
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Proteintech mouse mono
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Addgene inc puasp yfp rab4dn
Data and Software Availability
Puasp Yfp Rab4dn, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Addgene inc puasp yfp rab4
Data and Software Availability
Puasp Yfp Rab4, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ProSci Incorporated anti rab4
Data and Software Availability
Anti Rab4, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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92
Aviva Systems rab4
C. trachomatis recruits Rab14, <t>Rab4,</t> Rab22a, and Rab11a to the inclusion in JAWS-II DCs. JAWS-II DCs were infected with GFP- C. trachomatis L2 at MOI 100 for 24 h and analyzed by confocal microscopy. The intracellular distribution of (A, B) Rab14, (E, F) Rab4, (I, J) Rab22a and (M, N) Rab11a was evaluated in non-infected and infected cells. Endogenous Rab proteins were detected by indirect immunofluorescence using primary antibodies followed by its corresponding Cy3-conjugated secondary antibodies. Insets show a chlamydial inclusion magnification for each marker. DAPI was used to stain DNA. Bars represent 10 um. Images are representative of three independent experiments and more than 20 images were analyzed for each Rab. (C, G, K, O) A representative chlamydial inclusion was transversely crossed with eight diameter lines to obtain an intensity histogram. Line graphs show the MFI from GFP and the corresponding Rab protein with the SD for each point. (D, H, L, P) Chlamydial inclusion was transversely crossed with a diameter line to obtain an intensity histogram for each Rab protein; and five points of each line corresponding to the cytoplasm (P1), inclusion membrane (P2), inside the inclusion (P3), inclusion membrane (P4), and cytoplasm (P5) were selected. Twenty cells were analyzed for each Rab protein. One-way ANOVA with Dunnett’s multiple comparison post-test were performed. ### P < 0.001, #### P < 0.0001, and ****P < 0.0001.
Rab4, supplied by Aviva Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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93
Santa Cruz Biotechnology anti rab4
C. trachomatis recruits Rab14, <t>Rab4,</t> Rab22a, and Rab11a to the inclusion in JAWS-II DCs. JAWS-II DCs were infected with GFP- C. trachomatis L2 at MOI 100 for 24 h and analyzed by confocal microscopy. The intracellular distribution of (A, B) Rab14, (E, F) Rab4, (I, J) Rab22a and (M, N) Rab11a was evaluated in non-infected and infected cells. Endogenous Rab proteins were detected by indirect immunofluorescence using primary antibodies followed by its corresponding Cy3-conjugated secondary antibodies. Insets show a chlamydial inclusion magnification for each marker. DAPI was used to stain DNA. Bars represent 10 um. Images are representative of three independent experiments and more than 20 images were analyzed for each Rab. (C, G, K, O) A representative chlamydial inclusion was transversely crossed with eight diameter lines to obtain an intensity histogram. Line graphs show the MFI from GFP and the corresponding Rab protein with the SD for each point. (D, H, L, P) Chlamydial inclusion was transversely crossed with a diameter line to obtain an intensity histogram for each Rab protein; and five points of each line corresponding to the cytoplasm (P1), inclusion membrane (P2), inside the inclusion (P3), inclusion membrane (P4), and cytoplasm (P5) were selected. Twenty cells were analyzed for each Rab protein. One-way ANOVA with Dunnett’s multiple comparison post-test were performed. ### P < 0.001, #### P < 0.0001, and ****P < 0.0001.
Anti Rab4, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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88
Proteintech rabbit anti rufy1 pab
C. trachomatis recruits Rab14, <t>Rab4,</t> Rab22a, and Rab11a to the inclusion in JAWS-II DCs. JAWS-II DCs were infected with GFP- C. trachomatis L2 at MOI 100 for 24 h and analyzed by confocal microscopy. The intracellular distribution of (A, B) Rab14, (E, F) Rab4, (I, J) Rab22a and (M, N) Rab11a was evaluated in non-infected and infected cells. Endogenous Rab proteins were detected by indirect immunofluorescence using primary antibodies followed by its corresponding Cy3-conjugated secondary antibodies. Insets show a chlamydial inclusion magnification for each marker. DAPI was used to stain DNA. Bars represent 10 um. Images are representative of three independent experiments and more than 20 images were analyzed for each Rab. (C, G, K, O) A representative chlamydial inclusion was transversely crossed with eight diameter lines to obtain an intensity histogram. Line graphs show the MFI from GFP and the corresponding Rab protein with the SD for each point. (D, H, L, P) Chlamydial inclusion was transversely crossed with a diameter line to obtain an intensity histogram for each Rab protein; and five points of each line corresponding to the cytoplasm (P1), inclusion membrane (P2), inside the inclusion (P3), inclusion membrane (P4), and cytoplasm (P5) were selected. Twenty cells were analyzed for each Rab protein. One-way ANOVA with Dunnett’s multiple comparison post-test were performed. ### P < 0.001, #### P < 0.0001, and ****P < 0.0001.
Rabbit Anti Rufy1 Pab, supplied by Proteintech, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
OriGene human rab4a
C. trachomatis recruits Rab14, <t>Rab4,</t> Rab22a, and Rab11a to the inclusion in JAWS-II DCs. JAWS-II DCs were infected with GFP- C. trachomatis L2 at MOI 100 for 24 h and analyzed by confocal microscopy. The intracellular distribution of (A, B) Rab14, (E, F) Rab4, (I, J) Rab22a and (M, N) Rab11a was evaluated in non-infected and infected cells. Endogenous Rab proteins were detected by indirect immunofluorescence using primary antibodies followed by its corresponding Cy3-conjugated secondary antibodies. Insets show a chlamydial inclusion magnification for each marker. DAPI was used to stain DNA. Bars represent 10 um. Images are representative of three independent experiments and more than 20 images were analyzed for each Rab. (C, G, K, O) A representative chlamydial inclusion was transversely crossed with eight diameter lines to obtain an intensity histogram. Line graphs show the MFI from GFP and the corresponding Rab protein with the SD for each point. (D, H, L, P) Chlamydial inclusion was transversely crossed with a diameter line to obtain an intensity histogram for each Rab protein; and five points of each line corresponding to the cytoplasm (P1), inclusion membrane (P2), inside the inclusion (P3), inclusion membrane (P4), and cytoplasm (P5) were selected. Twenty cells were analyzed for each Rab protein. One-way ANOVA with Dunnett’s multiple comparison post-test were performed. ### P < 0.001, #### P < 0.0001, and ****P < 0.0001.
Human Rab4a, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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85
OriGene rab4 rfp
C. trachomatis recruits Rab14, <t>Rab4,</t> Rab22a, and Rab11a to the inclusion in JAWS-II DCs. JAWS-II DCs were infected with GFP- C. trachomatis L2 at MOI 100 for 24 h and analyzed by confocal microscopy. The intracellular distribution of (A, B) Rab14, (E, F) Rab4, (I, J) Rab22a and (M, N) Rab11a was evaluated in non-infected and infected cells. Endogenous Rab proteins were detected by indirect immunofluorescence using primary antibodies followed by its corresponding Cy3-conjugated secondary antibodies. Insets show a chlamydial inclusion magnification for each marker. DAPI was used to stain DNA. Bars represent 10 um. Images are representative of three independent experiments and more than 20 images were analyzed for each Rab. (C, G, K, O) A representative chlamydial inclusion was transversely crossed with eight diameter lines to obtain an intensity histogram. Line graphs show the MFI from GFP and the corresponding Rab protein with the SD for each point. (D, H, L, P) Chlamydial inclusion was transversely crossed with a diameter line to obtain an intensity histogram for each Rab protein; and five points of each line corresponding to the cytoplasm (P1), inclusion membrane (P2), inside the inclusion (P3), inclusion membrane (P4), and cytoplasm (P5) were selected. Twenty cells were analyzed for each Rab protein. One-way ANOVA with Dunnett’s multiple comparison post-test were performed. ### P < 0.001, #### P < 0.0001, and ****P < 0.0001.
Rab4 Rfp, supplied by OriGene, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Data and Software Availability

Journal: Developmental cell

Article Title: Membrane flow drives an adhesion-independent amoeboid cell migration mode

doi: 10.1016/j.devcel.2018.05.029

Figure Lengend Snippet: Data and Software Availability

Article Snippet: Method Details DNA Constructs The following constructs were obtained from Addgene: mVenus-myosinIIA (#56389, Michael Davidson), GFP-rGBD (#26732, William Bement), mCherry-ezrin (#55043, Michael Davidson), mCherry-moesin (#55103, Michael Davidson), Venus-iLID-CaaX (#60411, Brian Kuhlman), mRFP-FKBP12-5ptpase (#67516, Tamas Balla), mRFP-FKBP12 (#67514, Tamas Balla), EYFP-Clathrin-19 (#56584, Michael Davidson), mCherry-Lifeact-7 (#54491, Michael Davidson), mApple-caveolin (#54872, Michael Davidson), pUASP-YFP-Rab4 (#37690, Matthew Scott), pUASP-YFP-Rab4DN (#37691, Matthew Scott).

Techniques: Software, Recombinant, Sequencing, RNA Sequencing Assay

Data and Software Availability

Journal: Developmental cell

Article Title: Membrane flow drives an adhesion-independent amoeboid cell migration mode

doi: 10.1016/j.devcel.2018.05.029

Figure Lengend Snippet: Data and Software Availability

Article Snippet: DNA Constructs The following constructs were obtained from Addgene: mVenus-myosinIIA (#56389, Michael Davidson), GFP-rGBD (#26732, William Bement), mCherry-ezrin (#55043, Michael Davidson), mCherry-moesin (#55103, Michael Davidson), Venus-iLID-CaaX (#60411, Brian Kuhlman), mRFP-FKBP12-5ptpase (#67516, Tamas Balla), mRFP-FKBP12 (#67514, Tamas Balla), EYFP-Clathrin-19 (#56584, Michael Davidson), mCherry-Lifeact-7 (#54491, Michael Davidson), mApple-caveolin (#54872, Michael Davidson), pUASP-YFP-Rab4 (#37690, Matthew Scott), pUASP-YFP-Rab4DN (#37691, Matthew Scott).

Techniques: Software, Recombinant, Sequencing, RNA Sequencing Assay

C. trachomatis recruits Rab14, Rab4, Rab22a, and Rab11a to the inclusion in JAWS-II DCs. JAWS-II DCs were infected with GFP- C. trachomatis L2 at MOI 100 for 24 h and analyzed by confocal microscopy. The intracellular distribution of (A, B) Rab14, (E, F) Rab4, (I, J) Rab22a and (M, N) Rab11a was evaluated in non-infected and infected cells. Endogenous Rab proteins were detected by indirect immunofluorescence using primary antibodies followed by its corresponding Cy3-conjugated secondary antibodies. Insets show a chlamydial inclusion magnification for each marker. DAPI was used to stain DNA. Bars represent 10 um. Images are representative of three independent experiments and more than 20 images were analyzed for each Rab. (C, G, K, O) A representative chlamydial inclusion was transversely crossed with eight diameter lines to obtain an intensity histogram. Line graphs show the MFI from GFP and the corresponding Rab protein with the SD for each point. (D, H, L, P) Chlamydial inclusion was transversely crossed with a diameter line to obtain an intensity histogram for each Rab protein; and five points of each line corresponding to the cytoplasm (P1), inclusion membrane (P2), inside the inclusion (P3), inclusion membrane (P4), and cytoplasm (P5) were selected. Twenty cells were analyzed for each Rab protein. One-way ANOVA with Dunnett’s multiple comparison post-test were performed. ### P < 0.001, #### P < 0.0001, and ****P < 0.0001.

Journal: Frontiers in Immunology

Article Title: Chlamydia trachomatis Infection Impairs MHC-I Intracellular Trafficking and Antigen Cross-Presentation by Dendritic Cells

doi: 10.3389/fimmu.2021.662096

Figure Lengend Snippet: C. trachomatis recruits Rab14, Rab4, Rab22a, and Rab11a to the inclusion in JAWS-II DCs. JAWS-II DCs were infected with GFP- C. trachomatis L2 at MOI 100 for 24 h and analyzed by confocal microscopy. The intracellular distribution of (A, B) Rab14, (E, F) Rab4, (I, J) Rab22a and (M, N) Rab11a was evaluated in non-infected and infected cells. Endogenous Rab proteins were detected by indirect immunofluorescence using primary antibodies followed by its corresponding Cy3-conjugated secondary antibodies. Insets show a chlamydial inclusion magnification for each marker. DAPI was used to stain DNA. Bars represent 10 um. Images are representative of three independent experiments and more than 20 images were analyzed for each Rab. (C, G, K, O) A representative chlamydial inclusion was transversely crossed with eight diameter lines to obtain an intensity histogram. Line graphs show the MFI from GFP and the corresponding Rab protein with the SD for each point. (D, H, L, P) Chlamydial inclusion was transversely crossed with a diameter line to obtain an intensity histogram for each Rab protein; and five points of each line corresponding to the cytoplasm (P1), inclusion membrane (P2), inside the inclusion (P3), inclusion membrane (P4), and cytoplasm (P5) were selected. Twenty cells were analyzed for each Rab protein. One-way ANOVA with Dunnett’s multiple comparison post-test were performed. ### P < 0.001, #### P < 0.0001, and ****P < 0.0001.

Article Snippet: The following primaries antibodies were used: rabbit anti-CT529 (gently provided by Agathe Subtil, Pasteur Institute, Paris, France), rabbit polyclonal anti-Rab14, Rab4 and Rab11a (Aviva Systems Biology), mouse anti-CD63 (Invitrogen), goat polyclonal anti-EEA1 (Santa Cruz), purified mouse anti-Lamp1, purified mouse anti-H-2K b and FITC-coupled mouse anti-H-2K b (BD Biosciences), mouse monoclonal anti-TfR H68.4 (Invitrogen), mouse monoclonal anti-Rab22a (Santa Cruz), rabbit anti-clathrin (Abcam), rabbit anti-OVA (Sigma-Aldrich), and rabbit anti-MOMP (generously provided by Ted Hackstadt, National Institutes of Health, USA).

Techniques: Infection, Confocal Microscopy, Immunofluorescence, Marker, Staining, Membrane, Comparison