Journal:

Article Title: pp32 Reduction Induces Differentiation of TSU-Pr1 Cells

doi:

Figure Lengend Snippet: Expression of IL-6 in TSU-Pr1 cells. TSU-Pr1 cells stably transfected with pp32 anti-sense express higher levels of IL-6 message as compared to parental TSU-Pr1 cells and vector-only control by RT-PCR analysis, which validates the cDNA microarray analysis (see Figure 6).

Article Snippet: Microarray Analysis of TSU-Pr1 Cell Lines This procedure was performed at The Johns Hopkins University Oncology Microarray facility by using a human glass 12K cDNA chip.

Techniques: Expressing, Stable Transfection, Transfection, Plasmid Preparation, Control, Reverse Transcription Polymerase Chain Reaction, Microarray

Journal: DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes

Article Title: In silico Analysis of 3′-End-Processing Signals in Aspergillus oryzae Using Expressed Sequence Tags and Genomic Sequencing Data

doi: 10.1093/dnares/dsr011

Figure Lengend Snippet: Profile of the A. oryzae poly(A) data set. ( A ) Frequency distribution of EST contigs based on the EST copy number. The EST copy number of each contig contained in the A. oryzae poly(A) data set was obtained from the A. oryzae EST database ( http://nribf2.nrib.go.jp/EST2/index.html ). Data on the total EST contigs were obtained from the study by Akao et al . ( B ) Gene expression levels determined by DNA microarray analysis. The fluorescence intensity of each gene was normalized to that of the histone H4 gene.

Article Snippet: The A. oryzae wild-type strain RIB40, which was used for genome sequencing analysis, was grown in sterilized wheat bran media (3.0 g wheat bran with 1.8 ml distilled water) at 30°C for 33 h. Total RNA extraction, mRNA preparation, and DNA microarray analysis were performed according to the methods of Tamano et al . Purified Cy3- or Cy5-labelled cDNA probes were hybridized using 12 K A. oryzae oligonucleotide microarrays (Fermlab, Tokyo, Japan).

Techniques: Gene Expression, Microarray, Fluorescence

Journal: DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes

Article Title: In silico Analysis of 3′-End-Processing Signals in Aspergillus oryzae Using Expressed Sequence Tags and Genomic Sequencing Data

doi: 10.1093/dnares/dsr011

Figure Lengend Snippet: A schematic representation of the alignment of 3′-end-processing signals in A. oryzae , yeast, and plants. The arrow indicates the cleavage and polyadenylation site.

Article Snippet: The A. oryzae wild-type strain RIB40, which was used for genome sequencing analysis, was grown in sterilized wheat bran media (3.0 g wheat bran with 1.8 ml distilled water) at 30°C for 33 h. Total RNA extraction, mRNA preparation, and DNA microarray analysis were performed according to the methods of Tamano et al . Purified Cy3- or Cy5-labelled cDNA probes were hybridized using 12 K A. oryzae oligonucleotide microarrays (Fermlab, Tokyo, Japan).

Techniques:

Journal: DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes

Article Title: In silico Analysis of 3′-End-Processing Signals in Aspergillus oryzae Using Expressed Sequence Tags and Genomic Sequencing Data

doi: 10.1093/dnares/dsr011

Figure Lengend Snippet: Comparison of protein factors involved in pre-mRNA 3′-end-processing between Aspergillus oryzae , yeast, plants, and human

Article Snippet: The A. oryzae wild-type strain RIB40, which was used for genome sequencing analysis, was grown in sterilized wheat bran media (3.0 g wheat bran with 1.8 ml distilled water) at 30°C for 33 h. Total RNA extraction, mRNA preparation, and DNA microarray analysis were performed according to the methods of Tamano et al . Purified Cy3- or Cy5-labelled cDNA probes were hybridized using 12 K A. oryzae oligonucleotide microarrays (Fermlab, Tokyo, Japan).

Techniques: Comparison

Journal: Sensors (Basel, Switzerland)

Article Title: An Aptamer-Based Biosensor for Direct, Label-Free Detection of Melamine in Raw Milk

doi: 10.3390/s18103227

Figure Lengend Snippet: A schematic representation of the design of an aptamer-DNAzyme conjugated biosensor for microarray screening. The designed biosensor comprises an aptamer region (red), a DNAzyme region (blue), an internal loop region (cyan), two block sequences (gray) for the aptamer and the DNAzyme, and a linker region, immobilized on the microarray electrode.

Article Snippet: The 1200 unique sequences, 1180 for sensor screening, and 20 as control sequences, were randomly located on the 12K ElectraSense microarray chip purchased from Recenttec K.K. (Tokyo, Japan), with ten replicates.

Techniques: Microarray, Blocking Assay