Journal: BMC Pulmonary Medicine

Article Title: Extracellular microRNA-21 and microRNA-26a increase in body fluids from rats with antigen induced pulmonary inflammation and children with recurrent wheezing

doi: 10.1186/s12890-016-0216-2

Figure Lengend Snippet: Clinical implication of plasma miR-21 and miR-26a in childhood wheezing. a Total IgE in plasma detected using ELISA method. b ROC curves of plasma total IgE, plasma miR-21 and miR-26a for differentiation of childhood wheezing. Differences among three groups were analyzed by Kruskal-Wallis test followed Mann-Whitney test for difference between groups according to data characteristics. *: p < 0.05, **: p < 0.01, ***: p < 0.001

Article Snippet: Human total IgE was detected using an ELISA quantitation set (Bethyl Laboratory, USA, Cat No.E80-108).

Techniques: Clinical Proteomics, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

Journal: Acta Biomaterialia

Article Title: Multipolymer microsphere delivery of SARS-CoV-2 antigens

doi: 10.1016/j.actbio.2022.12.043

Figure Lengend Snippet: Humoral immune response to MS-Ag administration in SD rats. (a) Experimental outline for the blood and organ collection, 7- and 28-days post-IM injection of SD rats with either saline, empty MS, or MS-Ag. Splenocytes were subjected to flow cytometry. Blood was analyzed for T-cell subsets and toxicity profiling, (b) Enzyme linked immunosorbent assay (ELISA) for (b) anti-SARS CoV2 S-RBD IgG and (c) Nucleoprotein (N) IgG response showed significant IgG production at day 28 for anti-SARS-CoV-2 S- RBD IgG and N-IgG. Statistical analysis was done by Two-way ANOVA and Tukey's post- hoc tests for multiple comparisons, (****P < 0.0001).

Article Snippet: For these assays rat, SARS-CoV-2 IgG to Spike RBD Protein (SKU: KBVH015-22, Eagle Biosciences, Amherst NH, USA) and Rat Anti-SARS-CoV-2 IgG to N (SKU: KBVH015-23, Eagle Biosciences) were applied according to the manufacturer's instructions.

Techniques: Injection, Flow Cytometry, Enzyme-linked Immunosorbent Assay