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Image Search Results
Journal: Nucleic Acids Research
Article Title: 5΄-Vinylphosphonate improves tissue accumulation and efficacy of conjugated siRNAs in vivo
doi: 10.1093/nar/gkx507
Figure Lengend Snippet: 5΄ phosphorylation of hsiRNAs increases activity in vitro . ( A ) Cartoon of hsiRNA chemical scaffold. ( B ) Graphs showing the levels of PPIB (left) or HTT mRNA (right) in HeLa cells treated with 5΄-hydroxyl (5΄-OH) or 5΄-phosphate (5΄-P) hsiRNA PPIB (left) or hsiRNA HTT (right) or non-targeting control (NTC) hsiRNAs. mRNA levels measured by QuantiGene ® 2.0 assay are normalized to the level of a control HPRT mRNA and shown as percent of the untreated (UNT) control. The IC50 for the experimental hsiRNAs are indicated in the legend. N = 3 for each datapoint.
Article Snippet: Tissue punches were lysed and mRNA quantification was performed using the
Techniques: Activity Assay, In Vitro
Journal: Nucleic Acids Research
Article Title: 5΄-Vinylphosphonate improves tissue accumulation and efficacy of conjugated siRNAs in vivo
doi: 10.1093/nar/gkx507
Figure Lengend Snippet: 5΄ -(E)- vinylphosphonate modification enhances hsiRNA efficacy in liver, kidneys and heart column scatter plots showing Ppib or Htt mRNA levels in the livers, kidneys and hearts of mice ( n = 5 per group) treated with 5΄-hydroxyl (5΄-OH), 5΄-phosphate (5΄-P), or and 5΄-( E )-vinylphosphonate (5΄-VP) hsiRNAs by intravenous (IV) of subcutaneous (SC) injection. mRNA levels measured by QuantiGene ® 2.0 assay, were normalized to Hprt mRNA and expressed as percent of mRNA levels in PBS-treated animals. NTC, non-targeting control. T, targeting hsiRNA. Significance calculated by ANOVA with Bonferroni΄ s correction: ns, non-significant; * P ≤ 0.05; ** P ≤ 0.01; *** P ≤ 0.001 and **** P ≤ 0.0001.
Article Snippet: Tissue punches were lysed and mRNA quantification was performed using the
Techniques: Modification, Injection