Journal: Nature Reviews. Drug Discovery

Article Title: The clinical impact of glycobiology: targeting selectins, Siglecs and mammalian glycans

doi: 10.1038/s41573-020-00093-1

Figure Lengend Snippet: Selected clinical trials of glycobiology-targeted therapeutics a

Article Snippet: Carbohydrate vaccine Adagloxad simolenin (OPT-822; Globo H–KLH/QS-21 vaccine); OBI Pharma and Memorial Sloan Kettering Cancer Center , Breast cancer , Phase II/III completed 2019 , No improvement in survival; progression-free and overall survival did improve in patients with serologic response , NCT01516307 (ref. ) .

Techniques: Clinical Proteomics, Cream, Activation Assay, Recombinant, Glycoproteomics, Vaccines, Produced, Generated, Expressing, Imaging

Journal:

Article Title: Adjuvant-dependent modulation of Th 1 and Th 2 responses to immunization with ?-amyloid

doi: 10.1093/intimm/dxg049

Figure Lengend Snippet: Mapping of T cell epitopes in mice immunized and boosted 3 times with immunogen mixed with alum, TMG, QS21 or CFA/IFA. (A) Proliferation of T cells (stimulation index) was determined after in vitro activation of individual mouse splenocyte cultures (SD represent n = 8) with 10 μg/ml of overlapping peptides or Aβ40. (B) Proliferation of T cells was determined in five mice immunized with Aβ42 formulated in CFA/IFA. The stimulation index was determined after in vitro stimulation of individual mouse splenocyte cultures (n = 5) with 10 μg/ml of Aβ28 or Aβ40.

Article Snippet: However, only splenocytes from mice immunized with immunogen and QS21 or CFA produced significant amounts of IFN-γ, a T h 1 lymphokine. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window caption a7 Detection of IFN-γ (T h 1) and IL-4 (T h 2) lymphokines, and TNF-α pro-inflammatory cytokine in mice immunized and boosted 3 times with Aβ 42 formulated in alum, QS21 or CFA/IFA.

Techniques: In Vitro, Activation Assay

Journal:

Article Title: Adjuvant-dependent modulation of Th 1 and Th 2 responses to immunization with ?-amyloid

doi: 10.1093/intimm/dxg049

Figure Lengend Snippet: Detection of IFN-γ (Th1) and IL-4 (Th2) lymphokines, and TNF-α pro-inflammatory cytokine in mice immunized and boosted 3 times with Aβ42 formulated in alum, QS21 or CFA/IFA. Splenocytes from immunized mice (2 × 105/well) were incubated with Aβ40 peptide or medium alone in ELISPOT plates as described in Methods. Colored spots were counted after 24 h incubation. ANOVA has been used to compare production of each cytokine in the culture of splenocytes incubated with (experiment) or without (control) Aβ40 peptide (see Methods). Results are shown as mean number of spots/106 cells ± SD for stimulated (closed bars) versus unstimulated (open bars).

Article Snippet: However, only splenocytes from mice immunized with immunogen and QS21 or CFA produced significant amounts of IFN-γ, a T h 1 lymphokine. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window caption a7 Detection of IFN-γ (T h 1) and IL-4 (T h 2) lymphokines, and TNF-α pro-inflammatory cytokine in mice immunized and boosted 3 times with Aβ 42 formulated in alum, QS21 or CFA/IFA.

Techniques: Incubation, Enzyme-linked Immunospot, Control