Journal: International Journal of Nanomedicine

Article Title: Aptamer Functionalized Liposomes Co-Loaded with Exenatide-4 and Coenzyme Q10 Ameliorate Type 2 Diabetes Mellitus by Improving Pancreatic β Cell Function

doi: 10.2147/IJN.S510240

Figure Lengend Snippet: Orthogonal Experimental Design and Results of CoQ10-Lip

Article Snippet: CoQ10 and Ext-4 were purchased from Med Chem Express (Guangzhou, China); Phosphatidylcholine (PC), cholesterol (Chol), and streptozotocin (STZ) were purchased from Aladdin Biochemical Technology (Shanghai, China).

Techniques:

Journal: International Journal of Nanomedicine

Article Title: Aptamer Functionalized Liposomes Co-Loaded with Exenatide-4 and Coenzyme Q10 Ameliorate Type 2 Diabetes Mellitus by Improving Pancreatic β Cell Function

doi: 10.2147/IJN.S510240

Figure Lengend Snippet: Trend chart of factor levels in CoQ10-Lip.

Article Snippet: CoQ10 and Ext-4 were purchased from Med Chem Express (Guangzhou, China); Phosphatidylcholine (PC), cholesterol (Chol), and streptozotocin (STZ) were purchased from Aladdin Biochemical Technology (Shanghai, China).

Techniques:

Journal: International Journal of Nanomedicine

Article Title: Aptamer Functionalized Liposomes Co-Loaded with Exenatide-4 and Coenzyme Q10 Ameliorate Type 2 Diabetes Mellitus by Improving Pancreatic β Cell Function

doi: 10.2147/IJN.S510240

Figure Lengend Snippet: Characterization of (E+Q)-Lip-Apt (n=3) ( \documentclass[12pt]{minimal} \usepackage{wasysym} \usepackage[substack]{amsmath} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage[mathscr]{eucal} \usepackage{mathrsfs} \DeclareFontFamily{T1}{linotext}{} \DeclareFontShape{T1}{linotext}{m}{n} {linotext }{} \DeclareSymbolFont{linotext}{T1}{linotext}{m}{n} \DeclareSymbolFontAlphabet{\mathLINOTEXT}{linotext} \begin{document}$\overline {x } \pm {\ }s$\end{document} )

Article Snippet: CoQ10 and Ext-4 were purchased from Med Chem Express (Guangzhou, China); Phosphatidylcholine (PC), cholesterol (Chol), and streptozotocin (STZ) were purchased from Aladdin Biochemical Technology (Shanghai, China).

Techniques: Zeta Potential Analyzer

Journal: International Journal of Nanomedicine

Article Title: Aptamer Functionalized Liposomes Co-Loaded with Exenatide-4 and Coenzyme Q10 Ameliorate Type 2 Diabetes Mellitus by Improving Pancreatic β Cell Function

doi: 10.2147/IJN.S510240

Figure Lengend Snippet: Factor Levels of CoQ10-Lip

Article Snippet: CoQ10 and Ext-4 were purchased from Med Chem Express (Guangzhou, China); Phosphatidylcholine (PC), cholesterol (Chol), and streptozotocin (STZ) were purchased from Aladdin Biochemical Technology (Shanghai, China).

Techniques:

Journal: Scientific Reports

Article Title: TIPE2 suppresses ferroptosis and pro-inflammatory polarization in macrophages triggered by SARS-CoV-2 spike protein

doi: 10.1038/s41598-025-14235-1

Figure Lengend Snippet: TIPE2 regulates ferroptosis-related pathways in THP-1 macrophages following SARS-CoV-2 spike protein stimulation. a , b Expression of ferroptosis-related genes in THP−1 macrophages by qRT-PCR and Western blotting. the ferroptosis-related proteins including GPx4, SLC7A11,FSP1 and ACSL4. Cropped blot is shown for clarity, retaining relevant bands. The uncropped, full-length blot with visible edges is included in Supplementary Figure S3, 4. c Cell viability was evaluated by CCK8 assay in TIPE2-transfected or control THP-1 macrophages treated with S protein alone or in combination with Fer-1 (2µM). d-h Quantification of intracellular levels of ferroptosis-associated metabolic indicators, including ferrous iron (Fe² + ), Coenzyme Q10 (CoQ10), NADP⁺/NADPH ratio, malondialdehyde (MDA), and GSH/GSSG ratio in THP-1 macrophages under the indicated treatments. i Representative fluorescence microscopy images showing lipid ROS accumulation in THP-1 macrophages labeled with specific probes. Nuclei are stained blue (Hoechst), lipid ROS appear in green (FITC channel), and merged images are presented. Data are representative of at least three independent experiments. Student’s t-test evaluated differences between two groups, and one-way ANOVA with post-hoc testing (LSD or Tamhane’s T2) was used for comparisons among three groups. (* P < 0.05, ** P < 0.01, *** P < 0.001)

Article Snippet: Intracellular coenzyme (Co) Q10 levels were determined using a human CoQ10 ELISA kit (CUSABIO, China).

Techniques: Expressing, Quantitative RT-PCR, Western Blot, CCK-8 Assay, Transfection, Control, Fluorescence, Microscopy, Labeling, Staining

Journal: iScience

Article Title: Phospholipase PLA2G7 is complementary to GPX4 in mitigating punicic-acid-induced ferroptosis in prostate cancer cells

doi: 10.1016/j.isci.2024.109774

Figure Lengend Snippet:

Article Snippet: Coenzyme Q10 , Selleck chemicals , Cat# S2398.

Techniques: Virus, Control, Expressing, Plasmid Preparation, Recombinant, Western Blot, Transfection, Saline, Protease Inhibitor, cDNA Synthesis, Bicinchoninic Acid Protein Assay, Software, RNA Sequencing

Journal: Nature medicine

Article Title: Regulation of the hepatitis C virus RNA replicase by endogenous lipid peroxidation

doi: 10.1038/nm.3610

Figure Lengend Snippet: Differential regulation of HCV strains by SPHK2-mediated lipid peroxidation. ( a ) Dose-dependent effects of PUFAs on H77S.3/GLuc and HJ3-5/GLuc RNAs in Huh-7.5 cells. Data shown represent percent GLuc activity secreted between 48–72 h relative to DMSO control. ( b ) Growth kinetics of H77S.3/GLuc and HJ3-5/GLuc RNAs in the presence of 50 μM PUFAs. Data shown are mean ± s.e.m. of GLuc activity in supernatant fluids of two replicate cultures. ( c ) Cells transfected with HCV RNAs encoding GLuc were treated with DMSO, 100 μM LA or 100 μM LA plus 1 μM SKI. Data shown represent percent GLuc activity secreted between 48–72 h relative to DMSO control. L.O.D. = limit of detection. ( d ) Effect of 1 μM SKI, 1 μM VE, 100 μM CoQ10 or 50 μM ARA or DHA, on intracellular malondialdehyde (MDA) abundance in cells transfected with the indicated HCV/GLuc RNAs at 72 h. MDA was significantly increased by PUFAs and reduced by SKI or lipophilic antioxidants ( P < 0.01). ( e ) Analysis of 8-isoprostane abundance in cells electroporated with the indicated HCV RNAs and grown in the presence of 1 μM SKI or VE, or 50 μM LA with or without 1 μM SKI and VE for 48 h. ( f ) Effect of siRNA targeting SPHK isoforms (see ) on MDA accumulation after treatment with increasing concentrations of LA (6.25, 12.5, 25, 50, 100 μM) for 24 h (left panel). MDA levels in Huh-7.5 cells treated with increasing concentrations of LA in the presence of DMSO or 1 μM SKI (right panel). ( g ) Effects of increasing concentrations of VE (left), 1 μM VE alone, or 1 μM VE plus 1 μM SKI (right) on replication of H77S.3/GLuc and HJ3-5/GLuc RNAs. Data shown represent GLuc secreted between 48–72 h relative to DMSO control. ( h ) GLuc secretion from Huh-7.5 cells transfected as in a and treated with 10 μM CuOH with or without 10 μM VE. ( i ) Influence of SKI or VE (each 1 μM) on replication of H77S.3 and HJ3-5 viruses expressing GLuc in cells cultured in the presence or absence of 10% FBS. Medium containing 10% FBS was replaced with FBS-free or 10% FBS media containing SKI, VE or DMSO 6 h after RNA transfection. Data shown represent mean GLuc activity ± s.e.m. from two ( a–f,i ) or three ( g,h ) independent experiments. * P < 0.05, ** P < 0.01.

Article Snippet: Vitamin E (α-, rac-β-, and γ-tocopherols), 4-deoxypyridoxine hydrochloride (DOP), coenzyme Q10, butylated hydroxytoluene, N-acetyl-L-cysteine, diphenyleneiodonium chloride, oleic acid, and cyclosporine A were from Sigma-Aldrich. nSMase spiroepoxide and cumene hydroperoxide were from Santa Cruz Biotechnology, D609 was from Enzo Life Sciences, and sofosbuvir (PSI-7977) was from Chemscene.

Techniques: Activity Assay, Control, Transfection, Expressing, Cell Culture

Journal: Frontiers in Immunology

Article Title: Silencing of circRERE(4-5) inhibits ONECUT2-mediated tumorigenesis and metastasis in gastric cancer

doi: 10.3389/fimmu.2026.1686702

Figure Lengend Snippet: CircRERE(4-5) upregulates ONECUT2 by sponging miR-571. (A) qRT–PCR demonstrating the enrichment of circRNAs in a representative anti-AGO2 RIP assay conducted in GC cells. (B) Venn diagram illustrating the overlap of candidate miRNAs predicted to interact with circRERE(4-5) by two open-access databases. (C) qRT–PCR showing the enrichment of miRNAs following circRERE(4-5) pull-down in lysates of GC cells. (D) Venn diagram depicting the intersection of candidate mRNAs targeted by miR-571 as predicted by three open-access databases. (E) qRT–PCR analysis of ONECUT2 mRNA expression in GC cells under control conditions (sh-NC) or with circRERE(4-5) knockdown (sh-circRERE(4-5)). (F) qRT–PCR analysis of ONECUT2 mRNA expression in GC cells transfected with control inhibitors or miR-571 inhibitors. (G) Western blotting revealing ONECUT2 protein levels in GC cells under control conditions (sh-NC) or with circRERE(4-5) knockdown (sh-circRERE(4-5)), with GAPDH serving as a loading control. (H) Western blotting showing ONECUT2 protein levels in GC cells transfected with control inhibitors or miR-571 inhibitors. (I) qRT–PCR analysis of ONECUT2 mRNA expression in AGS cells under control conditions (sh-NC), after circRERE(4-5) knockdown (sh-circRERE(4-5)), or following cotransfection with sh-circRERE(4-5) and miR-571 inhibitors. (J) Representative western blot of ONECUT2 in AGS cells under control conditions (sh-NC), after circRERE(4-5) knockdown (sh-circRERE(4-5)), or following cotransfection with sh-circRERE(4-5) and miR-571 inhibitors. (K) Sequence alignment analysis highlighting the binding sites of circRERE(4-5) and ONECUT2 3’ UTR on miR-571. Data are presented as the mean ± SD. P -values were calculated using a two-tailed unpaired Student’s t -test (E, F, I) ; ** P < 0.01, *** P < 0.001. See also Supplementary Figure 4 .

Article Snippet: The antibodies used were anti-ONECUT2 (Proteintech, #21916-1-AP; 1:1,000), anti-AGO2 (Proteintech, # 67934-1-Ig; 1:1,000), anti-RERE (abcam, ab217756; 1:1,000), and anti-GAPDH (Proteintech, #10494–1-AP; 1:6,000).

Techniques: Quantitative RT-PCR, Expressing, Control, Knockdown, Transfection, Western Blot, Cotransfection, Sequencing, Binding Assay, Two Tailed Test