Journal: Journal of Cancer

Article Title: CC-115 Mediates GSDME-Dependent Pyroptosis in Lung Adenocarcinoma Through the Akt/Bax Pathway

doi: 10.7150/jca.83175

Figure Lengend Snippet: CC-115 induces the pyroptosis of LUAD cells. ( A ) Representative images of LUAD cells treated with CC-115 (scale bar, 50 μm). ( B, C ) Results of the ELISA assay used to quantify LDH release and IL-1β secretion in LUAD cells. ( D ) Flow cytometry plots showing the percentage of Annexin V PE and 7-AAD double-positive LUAD cells. ( E ) Western blotting of pyroptosis markers in LUAD cells treated with CC-115. ** P < 0.01, *** P < 0.001. Ctl: control.

Article Snippet: The Pyroptosis Compound Library (L7400), CC-115 (S7891), and the Akt activator SC79 (S7863) were purchased from Selleck Chemicals (Houston, TX, USA).

Techniques: Enzyme-linked Immunosorbent Assay, Flow Cytometry, Western Blot, Control

Journal: Journal of Cancer

Article Title: CC-115 Mediates GSDME-Dependent Pyroptosis in Lung Adenocarcinoma Through the Akt/Bax Pathway

doi: 10.7150/jca.83175

Figure Lengend Snippet: GSDME knockdown abrogates the pyroptosis-inducing effect of CC-115 in LUAD cells. ( A ) Knockdown efficiency of GSDME determined via qRT-PCR and western blotting. ( B ) Representative images of LUAD cells in the indicated groups (scale bar, 50 μm). ( C, D ) ELISA determination of the release of LDH and secretion of IL-1β in the indicated groups. ( E ) Flow cytometry plots showing the percentage of Annexin V PE and 7-AAD double-positive cells in the indicated groups. ( F ) Western blotting of pyroptosis markers in the indicated groups. ** P < 0.01, *** P < 0.001, ns: not significant.

Article Snippet: The Pyroptosis Compound Library (L7400), CC-115 (S7891), and the Akt activator SC79 (S7863) were purchased from Selleck Chemicals (Houston, TX, USA).

Techniques: Knockdown, Quantitative RT-PCR, Western Blot, Enzyme-linked Immunosorbent Assay, Flow Cytometry

Journal: Journal of Cancer

Article Title: CC-115 Mediates GSDME-Dependent Pyroptosis in Lung Adenocarcinoma Through the Akt/Bax Pathway

doi: 10.7150/jca.83175

Figure Lengend Snippet: CC-115 regulates GSDME-dependent pyroptosis through Akt/Bax signaling. ( A ) Western blotting of Akt, Bax, and Cyt-c in A549 cells treated with or without CC-115 (5 μM) and/or SC79 (5 μM). ( B ) Representative images of the mitochondrial membrane potential signal in A549 cells determined using the JC-10 assay in the indicated groups (scale bar, 50 μm). ( C ) Representative bright field images of A549 cells in the indicated groups (scale bar, 50 μm). ( D, E ) Release of LDH and secretion of IL-1β in A549 cells as determined using ELISA. ( F ) Analysis of caspase-3 and GSDME expression using western blotting in A549 cells in the indicated groups. *** P < 0.001.

Article Snippet: The Pyroptosis Compound Library (L7400), CC-115 (S7891), and the Akt activator SC79 (S7863) were purchased from Selleck Chemicals (Houston, TX, USA).

Techniques: Western Blot, Membrane, Enzyme-linked Immunosorbent Assay, Expressing

Journal: Journal of Cancer

Article Title: CC-115 Mediates GSDME-Dependent Pyroptosis in Lung Adenocarcinoma Through the Akt/Bax Pathway

doi: 10.7150/jca.83175

Figure Lengend Snippet: CC-115-induced GSDME-dependent pyroptosis is downstream of the Bax-mitochondrial intrinsic pathway. ( A ) Knockout efficiency of Bax determined via qRT-PCR and western blotting. ( B ) Representative images of the mitochondrial membrane potential of LUAD cells determined using the JC-10 assay in the indicated groups (scale bar, 50 μm). ( C, D ) Release of LDH and secretion of IL-1β in LUAD cells in the indicated groups. ( E ) Western blotting of GSDME-dependent pyroptosis markers in LUAD cells in the indicated groups. *** P < 0.001, ns: not significant.

Article Snippet: The Pyroptosis Compound Library (L7400), CC-115 (S7891), and the Akt activator SC79 (S7863) were purchased from Selleck Chemicals (Houston, TX, USA).

Techniques: Knock-Out, Quantitative RT-PCR, Western Blot, Membrane

Journal: Journal of Cancer

Article Title: CC-115 Mediates GSDME-Dependent Pyroptosis in Lung Adenocarcinoma Through the Akt/Bax Pathway

doi: 10.7150/jca.83175

Figure Lengend Snippet: CC-115 shows the antitumor effect through GSDME-dependent pyroptosis in vivo . ( A ) Images of nude mice with subcutaneous xenografts in the indicated groups. ( B ) Xenograft tumors isolated from sacrificed mice (28 days) with or without CC-115 treatment (2.5 mg/kg per day). ( C ) Average weights of xenograft tumors in the indicated groups. ( D ) Kinetics of tumor growth by volume of subcutaneous xenograft tumors. ( E ) Immunohistochemistry of p-Akt, Bax, cleaved caspase 3, and GSDME in subcutaneous mouse tumors from the NC vs. NC+CC-115 groups (scale bar, 100 μm). ( F, G ) Release of serum LDH and secretion of IL-1β in nude mice. ** P < 0.01, *** P < 0.001.

Article Snippet: The Pyroptosis Compound Library (L7400), CC-115 (S7891), and the Akt activator SC79 (S7863) were purchased from Selleck Chemicals (Houston, TX, USA).

Techniques: In Vivo, Isolation, Immunohistochemistry

Journal: Journal of Cancer

Article Title: CC-115 Mediates GSDME-Dependent Pyroptosis in Lung Adenocarcinoma Through the Akt/Bax Pathway

doi: 10.7150/jca.83175

Figure Lengend Snippet: Diagram of the proposed mechanism and function of CC-115 in LUAD. CC-115 exerts its function as a dual DNA-PK/mTOR inhibitor, suppressing the activity of Akt. This induces significant mitochondrial damage and triggers caspase-3 activation, which induces GSDME-dependent pyroptosis, ultimately affecting cell survival in lung adenocarcinoma.

Article Snippet: The Pyroptosis Compound Library (L7400), CC-115 (S7891), and the Akt activator SC79 (S7863) were purchased from Selleck Chemicals (Houston, TX, USA).

Techniques: Activity Assay, Activation Assay