Journal: Journal of thrombosis and haemostasis : JTH

Article Title: Human urotensin II induces tissue factor and cellular adhesion molecules expression in human coronary endothelial cells: an emerging role for urotensin II in cardiovascular disease.

doi: 10.1111/j.1538-7836.2008.02923.x

Figure Lengend Snippet: Fig. 2. Panel A: Dose-response effects of urotensin II (5, 10, 20 and 50 nM for 6 h) on TF protein levels in HCAECs, evaluated by Western blot analysis. Urotensin II caused a dose-response increase of TF protein. Tubulin served as loading control. Panel B: Dose-response effects of urotensin II (5, 10, 20 and 50 nM for 6 h) on TF expression in HCAECs determined by FACS analysis. Urotensin induced a dose-response increase in TF expression. LPS represents positive control experiments. Panel C: Control experiments performed by preincubating endothelial cells with cycloheximide (CE), DRB, with a mouse monoclonal antibody directed against human TF (A-TF), pyrrolidine dithio carbamate ammonium (PDTC), Bay 11-7082, with urantide (Ur), or with PKC inhibitor Go6983. Panel D: The HMG-CoA reductase inhibitor lovastatin (Lova) significantly prevented urotensin-induced TF expression. This effect was reverted by mevalonate (Meva). A lovastatin similar effect was obtained by Rho A inhibitor Y-27632 (Y 27). Each bar represents the mean ± SD of six different experiments. *P < 0.005 vs. urotensin II (U II).

Article Snippet: Additional control experiments included cells preincubated for 10 min with a mouse monoclonal antibody against human TF (5 lg mL)1, American Diagnostica Inc.) and cells preincubated for 30 min with urantide (Peptides International, Louisville, KT, USA; 10 nM), the most potent urotensin receptor antagonist compound so far reported in the rat isolated aorta, that is also endowed with high affinity at human UT receptors [22].

Techniques: Western Blot, Control, Expressing, Positive Control

Journal: Journal of thrombosis and haemostasis : JTH

Article Title: Human urotensin II induces tissue factor and cellular adhesion molecules expression in human coronary endothelial cells: an emerging role for urotensin II in cardiovascular disease.

doi: 10.1111/j.1538-7836.2008.02923.x

Figure Lengend Snippet: Fig. 4. Panel A: Dose-response effects of urotensin II (5, 10, 20 and 50 nM for 12 h) on adhesion molecules expression in HCAECs, determined by FACS analysis. Urotensin II induced expression of ICAM-1 and VCAM- 1 on cell surface. LPS represents positive control. Panel B: Pretreatment of endothelial cells with lovastatin (Lova), decreased the expression of ICAM-1 and of VCAM-1. Mevalonate (Meva) reverted Lova effects. The Rho A inhibitor Y 27632, urantide, urotensin II antagonist peptide, PDTC and Bay 11-7082, inhibitors of NF-jB activation, also decreased the expression of ICAM-1 and of VCAM-1. PKC inhibitor Go6983 did not affect urotensin-induced CAMs expression. Each bar represents the mean ± SD of six experiments. *P < 0.005 vs. urotensin (U II). Panel C: Effects of urotensin stimulation on leukocyte adhesiveness on HCAECs. Urotensin caused the significant increase of cell-adherent leukocytes. In cells pretreated with urantide, lovastatin and PDTC, this phenomenon was not observed. Each bar represents the mean ± SD of six experiments. *P < 0.005 vs. urotensin (U II).

Article Snippet: Additional control experiments included cells preincubated for 10 min with a mouse monoclonal antibody against human TF (5 lg mL)1, American Diagnostica Inc.) and cells preincubated for 30 min with urantide (Peptides International, Louisville, KT, USA; 10 nM), the most potent urotensin receptor antagonist compound so far reported in the rat isolated aorta, that is also endowed with high affinity at human UT receptors [22].

Techniques: Expressing, Positive Control, Activation Assay

Journal: Journal of thrombosis and haemostasis : JTH

Article Title: Human urotensin II induces tissue factor and cellular adhesion molecules expression in human coronary endothelial cells: an emerging role for urotensin II in cardiovascular disease.

doi: 10.1111/j.1538-7836.2008.02923.x

Figure Lengend Snippet: Fig. 6. Panel A. Effects of urotensin II on nuclear translocation of NF-jB. Increasing concentrations of urotensin II (5, 10, 20, nM, for 30¢) and LPS (positive control), induced a distinct shifted band while no shift could be observed in unstimulated, control cells. Lovastatin (Lova), urantide, PDTC and Bay 11-7082 (Panel C) significantly reduced NF-jB activation induced by urotensin II. Graphs are a summary of data from three separate experiments (mean ± SD; *P < 0.005 vs. U II); insert shows result of a representative experiment. Panel B. Activation of IjB. Elevated levels of IjB were detected at baseline, in unstimulated cells. Any changes in IjB-a levels were observed after cell pretreatment with urantide, with Lova or with PDTC. Data are representative of three separate experiments. Tubulin served as loading control. Top, result of a representative experiment; bottom, summary of data from three separate experiments (mean ± SD). Panel C. Effects of the Rho A inhibitor, Y 27632, the PKC inhibitor Go¨ 6983, and of NF-jB inhibitor Bay 11- 7082 on expression of NF-jB. Y 27632 as well as Bay 11 significantly reduced NF-jB activation induced by urotensin II. The PKC inhibitor Go¨ 6983 had no effects ob NF-jB activation. Top, result of a representative experiment; bottom, summary of data from three separate experiments (mean ± SD).

Article Snippet: Additional control experiments included cells preincubated for 10 min with a mouse monoclonal antibody against human TF (5 lg mL)1, American Diagnostica Inc.) and cells preincubated for 30 min with urantide (Peptides International, Louisville, KT, USA; 10 nM), the most potent urotensin receptor antagonist compound so far reported in the rat isolated aorta, that is also endowed with high affinity at human UT receptors [22].

Techniques: Translocation Assay, Positive Control, Control, Activation Assay, Expressing

Journal: Journal of Oral Biology and Craniofacial Research

Article Title: Efficacy of two gingival retraction systems on lateral gingival displacement: A prospective clinical study

doi: 10.1016/j.jobcr.2013.05.006

Figure Lengend Snippet: Gingival retraction systems (left: Ultrapak, Ultradent Products Inc., South Jordan, Utah; right: Stay-Put, Coltene Whaledent AG). Ultrapak gingival retraction system considered as conventional system and Roeko Stay-Put is a new system of retraction consists of ultrathin copper wire.

Article Snippet: Roeko Stay-Put showed slightly higher retraction than Ultrapak due to presence of ultrathin copper wire in the center of the cord.

Techniques:

Journal: Journal of Oral Biology and Craniofacial Research

Article Title: Efficacy of two gingival retraction systems on lateral gingival displacement: A prospective clinical study

doi: 10.1016/j.jobcr.2013.05.006

Figure Lengend Snippet: Traveling microscopic view of sectioned die of retracted gingival sulcus (Ultrapak). Retracted gingival sulcus was viewed under traveling microscope, distance from the abutment tooth to the crest of the free gingiva.

Article Snippet: Roeko Stay-Put showed slightly higher retraction than Ultrapak due to presence of ultrathin copper wire in the center of the cord.

Techniques: Microscopy

Journal: Journal of Oral Biology and Craniofacial Research

Article Title: Efficacy of two gingival retraction systems on lateral gingival displacement: A prospective clinical study

doi: 10.1016/j.jobcr.2013.05.006

Figure Lengend Snippet: Gingival retraction width by two different retraction systems.

Article Snippet: Roeko Stay-Put showed slightly higher retraction than Ultrapak due to presence of ultrathin copper wire in the center of the cord.

Techniques:

Journal: Journal of Oral Biology and Craniofacial Research

Article Title: Efficacy of two gingival retraction systems on lateral gingival displacement: A prospective clinical study

doi: 10.1016/j.jobcr.2013.05.006

Figure Lengend Snippet: Comparison of mean gingival retraction between two groups.

Article Snippet: Roeko Stay-Put showed slightly higher retraction than Ultrapak due to presence of ultrathin copper wire in the center of the cord.

Techniques: Comparison, Standard Deviation

Journal: Journal of Oral Biology and Craniofacial Research

Article Title: Efficacy of two gingival retraction systems on lateral gingival displacement: A prospective clinical study

doi: 10.1016/j.jobcr.2013.05.006

Figure Lengend Snippet: Qualitative comparison between group A (Ultrapak) and group B (Stay-Put).

Article Snippet: Roeko Stay-Put showed slightly higher retraction than Ultrapak due to presence of ultrathin copper wire in the center of the cord.

Techniques: Comparison

Journal: Foods

Article Title: Development and Application of a New QuEChERS Method in UHPLC-QqQ-MS/MS to Detect Seven Biogenic Amines in Chinese Wines

doi: 10.3390/foods8110552

Figure Lengend Snippet: MS parameters of seven BAs and the internal standard a .

Article Snippet: The BA reference standards of CAD, HEX, HIS, PEA, PUT, TRY and TYR were purchased from Dr. Ehrenstorfer (purity higher than 99%, Augsburg, Germany).

Techniques:

Journal: Foods

Article Title: Development and Application of a New QuEChERS Method in UHPLC-QqQ-MS/MS to Detect Seven Biogenic Amines in Chinese Wines

doi: 10.3390/foods8110552

Figure Lengend Snippet: The extracted ion chromatograms of seven biogenic amines (BAs) and the internal standard in a standard solution under positive mode ( a ), and total ion chromatograms and extracted overlaid dynamic multiple reaction monitoring (DMRM) chromatograms obtained from ultra-performance liquid chromatography coupled to a triple quadrupole mass spectrometry (UHPLC-QqQ-MS/MS) analysis in full-scan mode ( b ), (left) standard solution; (right) real wine sample (CAD: Cadaverine; PUT: Putrescine; HIS: Histamine; DAH: 1,7-diaminoheptane; TYR: Tyramine; PEA: Phenylethylamine; HEX: Hexylamine; TRY: Tryptamine).

Article Snippet: The BA reference standards of CAD, HEX, HIS, PEA, PUT, TRY and TYR were purchased from Dr. Ehrenstorfer (purity higher than 99%, Augsburg, Germany).

Techniques: Targeted Proteomics, Liquid Chromatography, Mass Spectrometry, Tandem Mass Spectroscopy

Journal: Foods

Article Title: Development and Application of a New QuEChERS Method in UHPLC-QqQ-MS/MS to Detect Seven Biogenic Amines in Chinese Wines

doi: 10.3390/foods8110552

Figure Lengend Snippet: Abundance and recoveries obtained by different QuEChERS procedures ( a ). the pH optimization; ( b ). the extraction solvent optimization; ( c ). the recovery percentage obtained by applying acetonitrile and trichloromethane-butanol solutions; ( d ). the amount and type of salt optimization; ( e ). the clean-up optimization; different letters in same BA are statistically significant p ˂ 0.05; CAD: Cadaverine; PUT: Putrescine; HIS: Histamine; TYR: Tyramine; PEA: Phenylethylamine; HEX: Hexylamine; TRY: Tryptamine).

Article Snippet: The BA reference standards of CAD, HEX, HIS, PEA, PUT, TRY and TYR were purchased from Dr. Ehrenstorfer (purity higher than 99%, Augsburg, Germany).

Techniques: Extraction, Solvent

Journal: Foods

Article Title: Development and Application of a New QuEChERS Method in UHPLC-QqQ-MS/MS to Detect Seven Biogenic Amines in Chinese Wines

doi: 10.3390/foods8110552

Figure Lengend Snippet: LODs, LOQs, linearity, precision and recovery of seven BAs a .

Article Snippet: The BA reference standards of CAD, HEX, HIS, PEA, PUT, TRY and TYR were purchased from Dr. Ehrenstorfer (purity higher than 99%, Augsburg, Germany).

Techniques:

Journal: Foods

Article Title: Development and Application of a New QuEChERS Method in UHPLC-QqQ-MS/MS to Detect Seven Biogenic Amines in Chinese Wines

doi: 10.3390/foods8110552

Figure Lengend Snippet: Relative distribution of all tested wine samples analyzed in this study referring to their BA concentrations (CAD: Cadaverine; PUT: Putrescine; HIS: Histamine; TYR: Tyramine; PEA: Phenylethylamine; HEX: Hexylamine).

Article Snippet: The BA reference standards of CAD, HEX, HIS, PEA, PUT, TRY and TYR were purchased from Dr. Ehrenstorfer (purity higher than 99%, Augsburg, Germany).

Techniques:

Journal: Foods

Article Title: Development and Application of a New QuEChERS Method in UHPLC-QqQ-MS/MS to Detect Seven Biogenic Amines in Chinese Wines

doi: 10.3390/foods8110552

Figure Lengend Snippet: BA occurrence in different types of wine samples.

Article Snippet: The BA reference standards of CAD, HEX, HIS, PEA, PUT, TRY and TYR were purchased from Dr. Ehrenstorfer (purity higher than 99%, Augsburg, Germany).

Techniques:

Journal: Foods

Article Title: Development and Application of a New QuEChERS Method in UHPLC-QqQ-MS/MS to Detect Seven Biogenic Amines in Chinese Wines

doi: 10.3390/foods8110552

Figure Lengend Snippet: Correlation analysis between BAs and wine parameters.

Article Snippet: The BA reference standards of CAD, HEX, HIS, PEA, PUT, TRY and TYR were purchased from Dr. Ehrenstorfer (purity higher than 99%, Augsburg, Germany).

Techniques: