pum1 Search Results


gene exp pum1 hs00206469 m1  (Thermo Fisher)
85
Thermo Fisher gene exp pum1 hs00206469 m1
Gene Exp Pum1 Hs00206469 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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proteintech 26256 1 ap ubr7 antibody  (Proteintech)
93
Proteintech proteintech 26256 1 ap ubr7 antibody
Proteintech 26256 1 Ap Ubr7 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pum1 qt00029421  (Thermo Fisher)
86
Thermo Fisher pum1 qt00029421
Pum1 Qt00029421, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
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gene exp pum1 hs00472881 m1  (Thermo Fisher)
87
Thermo Fisher gene exp pum1 hs00472881 m1
Gene Exp Pum1 Hs00472881 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 87 stars, based on 1 article reviews
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lab ppl2014  (Addgene inc)
93
Addgene inc lab ppl2014
Lab Ppl2014, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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gene exp pum1 hs00982775 m1  (Thermo Fisher)
86
Thermo Fisher gene exp pum1 hs00982775 m1
Gene Exp Pum1 Hs00982775 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
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pum1 rc201219 orf  (OriGene)
89
OriGene pum1 rc201219 orf
FOXM1 and TAF1 act as transcription factors for NANOS1 and NANOS3 downregulated genes. ( A ) Cumulative distribution analysis of differentially expressed genes upon NANOS3 RNA-Seq. Common differentially expressed mRNA distribution shown in blue and labeled as targets. The rest of the differentially expressed genes are shown in red and labeled as non-targets. ( B ) Common differentially expressed genes that are repressed by both NANOS3 and <t>PUM1</t> identified as downregulated (log2FC < 0) upon NANOS3 overexpression and upregulated (log2FC > 0) upon PUM1 knockdown. ( C ) iRegulon transcription factor prediction analysis for NANOS3/PUM1 common repressed genes. ( D ) Cell cycle related biological processes identified by gene ontology enrichment analysis for NANOS3-PUM1 common repressed genes.
Pum1 Rc201219 Orf, supplied by OriGene, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 89 stars, based on 1 article reviews
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ptyb3 pum1 hd  (Addgene inc)
92
Addgene inc ptyb3 pum1 hd
FOXM1 and TAF1 act as transcription factors for NANOS1 and NANOS3 downregulated genes. ( A ) Cumulative distribution analysis of differentially expressed genes upon NANOS3 RNA-Seq. Common differentially expressed mRNA distribution shown in blue and labeled as targets. The rest of the differentially expressed genes are shown in red and labeled as non-targets. ( B ) Common differentially expressed genes that are repressed by both NANOS3 and <t>PUM1</t> identified as downregulated (log2FC < 0) upon NANOS3 overexpression and upregulated (log2FC > 0) upon PUM1 knockdown. ( C ) iRegulon transcription factor prediction analysis for NANOS3/PUM1 common repressed genes. ( D ) Cell cycle related biological processes identified by gene ontology enrichment analysis for NANOS3-PUM1 common repressed genes.
Ptyb3 Pum1 Hd, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gene exp pum1 rn01180594 m1  (Thermo Fisher)
94
Thermo Fisher gene exp pum1 rn01180594 m1
FOXM1 and TAF1 act as transcription factors for NANOS1 and NANOS3 downregulated genes. ( A ) Cumulative distribution analysis of differentially expressed genes upon NANOS3 RNA-Seq. Common differentially expressed mRNA distribution shown in blue and labeled as targets. The rest of the differentially expressed genes are shown in red and labeled as non-targets. ( B ) Common differentially expressed genes that are repressed by both NANOS3 and <t>PUM1</t> identified as downregulated (log2FC < 0) upon NANOS3 overexpression and upregulated (log2FC > 0) upon PUM1 knockdown. ( C ) iRegulon transcription factor prediction analysis for NANOS3/PUM1 common repressed genes. ( D ) Cell cycle related biological processes identified by gene ontology enrichment analysis for NANOS3-PUM1 common repressed genes.
Gene Exp Pum1 Rn01180594 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
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94/100 stars
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gene exp pum1 mm01180596 m1  (Thermo Fisher)
94
Thermo Fisher gene exp pum1 mm01180596 m1
Quantitative PCR probes
Gene Exp Pum1 Mm01180596 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gene exp pum1 mm01180596 m1/product/Thermo Fisher
Average 94 stars, based on 1 article reviews
gene exp pum1 mm01180596 m1 - by Bioz Stars, 2026-03
94/100 stars
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Image Search Results


FOXM1 and TAF1 act as transcription factors for NANOS1 and NANOS3 downregulated genes. ( A ) Cumulative distribution analysis of differentially expressed genes upon NANOS3 RNA-Seq. Common differentially expressed mRNA distribution shown in blue and labeled as targets. The rest of the differentially expressed genes are shown in red and labeled as non-targets. ( B ) Common differentially expressed genes that are repressed by both NANOS3 and PUM1 identified as downregulated (log2FC < 0) upon NANOS3 overexpression and upregulated (log2FC > 0) upon PUM1 knockdown. ( C ) iRegulon transcription factor prediction analysis for NANOS3/PUM1 common repressed genes. ( D ) Cell cycle related biological processes identified by gene ontology enrichment analysis for NANOS3-PUM1 common repressed genes.

Journal: International Journal of Molecular Sciences

Article Title: Distinct Roles of NANOS1 and NANOS3 in the Cell Cycle and NANOS3-PUM1-FOXM1 Axis to Control G2/M Phase in a Human Primordial Germ Cell Model

doi: 10.3390/ijms23126592

Figure Lengend Snippet: FOXM1 and TAF1 act as transcription factors for NANOS1 and NANOS3 downregulated genes. ( A ) Cumulative distribution analysis of differentially expressed genes upon NANOS3 RNA-Seq. Common differentially expressed mRNA distribution shown in blue and labeled as targets. The rest of the differentially expressed genes are shown in red and labeled as non-targets. ( B ) Common differentially expressed genes that are repressed by both NANOS3 and PUM1 identified as downregulated (log2FC < 0) upon NANOS3 overexpression and upregulated (log2FC > 0) upon PUM1 knockdown. ( C ) iRegulon transcription factor prediction analysis for NANOS3/PUM1 common repressed genes. ( D ) Cell cycle related biological processes identified by gene ontology enrichment analysis for NANOS3-PUM1 common repressed genes.

Article Snippet: Transient overexpression was performed by transfecting cells with pCMV6-entry vector (OriGene) containing NANOS1 (#RC219123), NANOS3 (#RC221867), and PUM1 (#RC201219) ORF tagged with FLAG (DYKDDDDK) peptide in the C-terminus.

Techniques: RNA Sequencing Assay, Labeling, Over Expression

FOXM1 mRNA is regulated by the NANOS3/PUM1 post-transcriptional complex through its’ 3′UTR. ( A ) Western-blot visualization of GST pull-down of NANOS3 with and without RNase A. ( B ) Schematic representation of FOXM1 3′UTR containing the Pumilio binding element (PBE-UGUAHAUA) and 2 PBE-like motifs (containing core UGUA motif). ( C ) Dual luciferase assay to measure Renilla/Firefly ratio upon PUM1 knockdown and NANOS3 overexpression using 3′UTR of FOXM1 in the downstream of Renilla ORF. Standard error is visualized as error bars. * = p -value ≤ 0.05, *** = p -value ≤ 0.001.

Journal: International Journal of Molecular Sciences

Article Title: Distinct Roles of NANOS1 and NANOS3 in the Cell Cycle and NANOS3-PUM1-FOXM1 Axis to Control G2/M Phase in a Human Primordial Germ Cell Model

doi: 10.3390/ijms23126592

Figure Lengend Snippet: FOXM1 mRNA is regulated by the NANOS3/PUM1 post-transcriptional complex through its’ 3′UTR. ( A ) Western-blot visualization of GST pull-down of NANOS3 with and without RNase A. ( B ) Schematic representation of FOXM1 3′UTR containing the Pumilio binding element (PBE-UGUAHAUA) and 2 PBE-like motifs (containing core UGUA motif). ( C ) Dual luciferase assay to measure Renilla/Firefly ratio upon PUM1 knockdown and NANOS3 overexpression using 3′UTR of FOXM1 in the downstream of Renilla ORF. Standard error is visualized as error bars. * = p -value ≤ 0.05, *** = p -value ≤ 0.001.

Article Snippet: Transient overexpression was performed by transfecting cells with pCMV6-entry vector (OriGene) containing NANOS1 (#RC219123), NANOS3 (#RC221867), and PUM1 (#RC201219) ORF tagged with FLAG (DYKDDDDK) peptide in the C-terminus.

Techniques: Western Blot, Binding Assay, Luciferase, Over Expression

The NANOS3–PUM1–FOXM1 axis coordinates the expression of the cell cycle genes. ( A ) Dual luciferase assay to measure Renilla/Firefly ratio upon PUM1 knockdown and NANOS3 overexpression using 3′UTR of CCNA2 , KIF20B , and RAD21 in the downstream of Renilla ORF. ( B ) Relative fold change of FOXM1 binding events per 1000 cells for the promoter region of the target genes measured by ChIP-qPCR. ( C ) Differential correlation analysis of NANOS3 , PUM1 , and FOXM1 in publicly available healthy testis (GTEx) and testicular cancer datasets (TCGA). Each dot represents a single GTEx or TCGA dataset. Expression values are transformed as log(expression +1). Standard error is visualized as error bars. ** = p -value ≤ 0.01, *** = p -value ≤ 0.001.

Journal: International Journal of Molecular Sciences

Article Title: Distinct Roles of NANOS1 and NANOS3 in the Cell Cycle and NANOS3-PUM1-FOXM1 Axis to Control G2/M Phase in a Human Primordial Germ Cell Model

doi: 10.3390/ijms23126592

Figure Lengend Snippet: The NANOS3–PUM1–FOXM1 axis coordinates the expression of the cell cycle genes. ( A ) Dual luciferase assay to measure Renilla/Firefly ratio upon PUM1 knockdown and NANOS3 overexpression using 3′UTR of CCNA2 , KIF20B , and RAD21 in the downstream of Renilla ORF. ( B ) Relative fold change of FOXM1 binding events per 1000 cells for the promoter region of the target genes measured by ChIP-qPCR. ( C ) Differential correlation analysis of NANOS3 , PUM1 , and FOXM1 in publicly available healthy testis (GTEx) and testicular cancer datasets (TCGA). Each dot represents a single GTEx or TCGA dataset. Expression values are transformed as log(expression +1). Standard error is visualized as error bars. ** = p -value ≤ 0.01, *** = p -value ≤ 0.001.

Article Snippet: Transient overexpression was performed by transfecting cells with pCMV6-entry vector (OriGene) containing NANOS1 (#RC219123), NANOS3 (#RC221867), and PUM1 (#RC201219) ORF tagged with FLAG (DYKDDDDK) peptide in the C-terminus.

Techniques: Expressing, Luciferase, Over Expression, Binding Assay, Transformation Assay

Model of NANOS3-PUM1-FOXM1 axis regulating G2/M phase of the cell cycle in TCam-2 cell line. Created with BioRender.com.

Journal: International Journal of Molecular Sciences

Article Title: Distinct Roles of NANOS1 and NANOS3 in the Cell Cycle and NANOS3-PUM1-FOXM1 Axis to Control G2/M Phase in a Human Primordial Germ Cell Model

doi: 10.3390/ijms23126592

Figure Lengend Snippet: Model of NANOS3-PUM1-FOXM1 axis regulating G2/M phase of the cell cycle in TCam-2 cell line. Created with BioRender.com.

Article Snippet: Transient overexpression was performed by transfecting cells with pCMV6-entry vector (OriGene) containing NANOS1 (#RC219123), NANOS3 (#RC221867), and PUM1 (#RC201219) ORF tagged with FLAG (DYKDDDDK) peptide in the C-terminus.

Techniques:

Quantitative PCR probes

Journal: Journal of Lipid Research

Article Title: Evaluation of antisense oligonucleotide therapy targeting Hsd17b13 in a fibrosis mice model

doi: 10.1016/j.jlr.2024.100514

Figure Lengend Snippet: Quantitative PCR probes

Article Snippet: Pum1 , Mm01180596_m1.

Techniques: Real-time Polymerase Chain Reaction