pt2 Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Millipore pt2
    Pt2, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pt2/product/Millipore
    Average 99 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    pt2 - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    89
    Boston Scientific pt2
    Pt2, supplied by Boston Scientific, used in various techniques. Bioz Stars score: 89/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pt2/product/Boston Scientific
    Average 89 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    pt2 - by Bioz Stars, 2020-08
    89/100 stars
      Buy from Supplier

    90
    Addgene inc pt2 bh
    Comparative Analysis of Transfection Efficiency and Transgenic Rate Using the pFAR4 or the <t>pT2</t> Plasmid to Encode the Venus Gene HeLa cells were co-transfected with either a high or a low plasmid amount using the following constructs: pFAR4-ITRs-SV-Venus (2,984 bp, 300 or 50 ng) or an equimolar amount of pT2/SV-Venus (4,878 bp, 490 or 82 ng) either without (−SB) or plus SB100X transposase encoded by the pFAR4 plasmid (30 or 5 ng). Using the pGL3 empty vector, the total amount of plasmid was adjusted to 800 or 500 ng for the high or low conditions, respectively. (A) Data represent the mean number of fluorescent cells ± SD, obtained 2 days after six and four independent transfections for the high and low plasmid amounts, respectively. **p
    Pt2 Bh, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pt2 bh/product/Addgene inc
    Average 90 stars, based on 37 article reviews
    Price from $9.99 to $1999.99
    pt2 bh - by Bioz Stars, 2020-08
    90/100 stars
      Buy from Supplier

    88
    Boston Scientific antegrade pt2
    Comparative Analysis of Transfection Efficiency and Transgenic Rate Using the pFAR4 or the <t>pT2</t> Plasmid to Encode the Venus Gene HeLa cells were co-transfected with either a high or a low plasmid amount using the following constructs: pFAR4-ITRs-SV-Venus (2,984 bp, 300 or 50 ng) or an equimolar amount of pT2/SV-Venus (4,878 bp, 490 or 82 ng) either without (−SB) or plus SB100X transposase encoded by the pFAR4 plasmid (30 or 5 ng). Using the pGL3 empty vector, the total amount of plasmid was adjusted to 800 or 500 ng for the high or low conditions, respectively. (A) Data represent the mean number of fluorescent cells ± SD, obtained 2 days after six and four independent transfections for the high and low plasmid amounts, respectively. **p
    Antegrade Pt2, supplied by Boston Scientific, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antegrade pt2/product/Boston Scientific
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antegrade pt2 - by Bioz Stars, 2020-08
    88/100 stars
      Buy from Supplier

    85
    Boston Scientific long pt2 guidewire
    A-H) Recanalization of the chronic in-stent occlusion at the ostium of right vertebral artery. A) Posterior-anterior fluoroscopy detected a 70% stenosis at ostium of right vertebral artery. B) The stenosis was eliminated after stenting. C) A CTA performed six months after the procedure detected moderate in-stent restenosis (arrow). D) Another CTA performed 12 months after the procedure detected in-stent occlusion (arrow). E) A subsequent DSA confirmed in-stent occlusion. F) Different guidewires were attempted for passing through the occluded stent, and a <t>PT2</t> <t>guidewire</t> crossed the occlusion. G) The occluded stent was dilated with a 2.0x15mm balloon, and the post-dilatation angiography displayed partial open of the stent. H) The stent was further dilated with a larger 4.0x20 balloon, and the final angiography confirmed complete patency of the stent.
    Long Pt2 Guidewire, supplied by Boston Scientific, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/long pt2 guidewire/product/Boston Scientific
    Average 85 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    long pt2 guidewire - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

    pt2  (TaKaRa)
    88
    TaKaRa pt2
    A-H) Recanalization of the chronic in-stent occlusion at the ostium of right vertebral artery. A) Posterior-anterior fluoroscopy detected a 70% stenosis at ostium of right vertebral artery. B) The stenosis was eliminated after stenting. C) A CTA performed six months after the procedure detected moderate in-stent restenosis (arrow). D) Another CTA performed 12 months after the procedure detected in-stent occlusion (arrow). E) A subsequent DSA confirmed in-stent occlusion. F) Different guidewires were attempted for passing through the occluded stent, and a <t>PT2</t> <t>guidewire</t> crossed the occlusion. G) The occluded stent was dilated with a 2.0x15mm balloon, and the post-dilatation angiography displayed partial open of the stent. H) The stent was further dilated with a larger 4.0x20 balloon, and the final angiography confirmed complete patency of the stent.
    Pt2, supplied by TaKaRa, used in various techniques. Bioz Stars score: 88/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pt2/product/TaKaRa
    Average 88 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    pt2 - by Bioz Stars, 2020-08
    88/100 stars
      Buy from Supplier

    88
    Boston Scientific choice pt2 guidewire
    A-H) Recanalization of the chronic in-stent occlusion at the ostium of right vertebral artery. A) Posterior-anterior fluoroscopy detected a 70% stenosis at ostium of right vertebral artery. B) The stenosis was eliminated after stenting. C) A CTA performed six months after the procedure detected moderate in-stent restenosis (arrow). D) Another CTA performed 12 months after the procedure detected in-stent occlusion (arrow). E) A subsequent DSA confirmed in-stent occlusion. F) Different guidewires were attempted for passing through the occluded stent, and a <t>PT2</t> <t>guidewire</t> crossed the occlusion. G) The occluded stent was dilated with a 2.0x15mm balloon, and the post-dilatation angiography displayed partial open of the stent. H) The stent was further dilated with a larger 4.0x20 balloon, and the final angiography confirmed complete patency of the stent.
    Choice Pt2 Guidewire, supplied by Boston Scientific, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/choice pt2 guidewire/product/Boston Scientific
    Average 88 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    choice pt2 guidewire - by Bioz Stars, 2020-08
    88/100 stars
      Buy from Supplier

    88
    Thermo Fisher pt2
    A-H) Recanalization of the chronic in-stent occlusion at the ostium of right vertebral artery. A) Posterior-anterior fluoroscopy detected a 70% stenosis at ostium of right vertebral artery. B) The stenosis was eliminated after stenting. C) A CTA performed six months after the procedure detected moderate in-stent restenosis (arrow). D) Another CTA performed 12 months after the procedure detected in-stent occlusion (arrow). E) A subsequent DSA confirmed in-stent occlusion. F) Different guidewires were attempted for passing through the occluded stent, and a <t>PT2</t> <t>guidewire</t> crossed the occlusion. G) The occluded stent was dilated with a 2.0x15mm balloon, and the post-dilatation angiography displayed partial open of the stent. H) The stent was further dilated with a larger 4.0x20 balloon, and the final angiography confirmed complete patency of the stent.
    Pt2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pt2/product/Thermo Fisher
    Average 88 stars, based on 34 article reviews
    Price from $9.99 to $1999.99
    pt2 - by Bioz Stars, 2020-08
    88/100 stars
      Buy from Supplier

    92
    Addgene inc pt2 ltr7 gfp
    A-H) Recanalization of the chronic in-stent occlusion at the ostium of right vertebral artery. A) Posterior-anterior fluoroscopy detected a 70% stenosis at ostium of right vertebral artery. B) The stenosis was eliminated after stenting. C) A CTA performed six months after the procedure detected moderate in-stent restenosis (arrow). D) Another CTA performed 12 months after the procedure detected in-stent occlusion (arrow). E) A subsequent DSA confirmed in-stent occlusion. F) Different guidewires were attempted for passing through the occluded stent, and a <t>PT2</t> <t>guidewire</t> crossed the occlusion. G) The occluded stent was dilated with a 2.0x15mm balloon, and the post-dilatation angiography displayed partial open of the stent. H) The stent was further dilated with a larger 4.0x20 balloon, and the final angiography confirmed complete patency of the stent.
    Pt2 Ltr7 Gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pt2 ltr7 gfp/product/Addgene inc
    Average 92 stars, based on 28 article reviews
    Price from $9.99 to $1999.99
    pt2 ltr7 gfp - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    92
    Addgene inc pt2 svneo vector
    A-H) Recanalization of the chronic in-stent occlusion at the ostium of right vertebral artery. A) Posterior-anterior fluoroscopy detected a 70% stenosis at ostium of right vertebral artery. B) The stenosis was eliminated after stenting. C) A CTA performed six months after the procedure detected moderate in-stent restenosis (arrow). D) Another CTA performed 12 months after the procedure detected in-stent occlusion (arrow). E) A subsequent DSA confirmed in-stent occlusion. F) Different guidewires were attempted for passing through the occluded stent, and a <t>PT2</t> <t>guidewire</t> crossed the occlusion. G) The occluded stent was dilated with a 2.0x15mm balloon, and the post-dilatation angiography displayed partial open of the stent. H) The stent was further dilated with a larger 4.0x20 balloon, and the final angiography confirmed complete patency of the stent.
    Pt2 Svneo Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pt2 svneo vector/product/Addgene inc
    Average 92 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    pt2 svneo vector - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    94
    Boston Scientific hydrophilic pt2
    A-H) Recanalization of the chronic in-stent occlusion at the ostium of right vertebral artery. A) Posterior-anterior fluoroscopy detected a 70% stenosis at ostium of right vertebral artery. B) The stenosis was eliminated after stenting. C) A CTA performed six months after the procedure detected moderate in-stent restenosis (arrow). D) Another CTA performed 12 months after the procedure detected in-stent occlusion (arrow). E) A subsequent DSA confirmed in-stent occlusion. F) Different guidewires were attempted for passing through the occluded stent, and a <t>PT2</t> <t>guidewire</t> crossed the occlusion. G) The occluded stent was dilated with a 2.0x15mm balloon, and the post-dilatation angiography displayed partial open of the stent. H) The stent was further dilated with a larger 4.0x20 balloon, and the final angiography confirmed complete patency of the stent.
    Hydrophilic Pt2, supplied by Boston Scientific, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hydrophilic pt2/product/Boston Scientific
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hydrophilic pt2 - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

    92
    MathWorks Inc pt2 element
    A-H) Recanalization of the chronic in-stent occlusion at the ostium of right vertebral artery. A) Posterior-anterior fluoroscopy detected a 70% stenosis at ostium of right vertebral artery. B) The stenosis was eliminated after stenting. C) A CTA performed six months after the procedure detected moderate in-stent restenosis (arrow). D) Another CTA performed 12 months after the procedure detected in-stent occlusion (arrow). E) A subsequent DSA confirmed in-stent occlusion. F) Different guidewires were attempted for passing through the occluded stent, and a <t>PT2</t> <t>guidewire</t> crossed the occlusion. G) The occluded stent was dilated with a 2.0x15mm balloon, and the post-dilatation angiography displayed partial open of the stent. H) The stent was further dilated with a larger 4.0x20 balloon, and the final angiography confirmed complete patency of the stent.
    Pt2 Element, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pt2 element/product/MathWorks Inc
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    pt2 element - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    93
    Addgene inc pt2 tetr
    A-H) Recanalization of the chronic in-stent occlusion at the ostium of right vertebral artery. A) Posterior-anterior fluoroscopy detected a 70% stenosis at ostium of right vertebral artery. B) The stenosis was eliminated after stenting. C) A CTA performed six months after the procedure detected moderate in-stent restenosis (arrow). D) Another CTA performed 12 months after the procedure detected in-stent occlusion (arrow). E) A subsequent DSA confirmed in-stent occlusion. F) Different guidewires were attempted for passing through the occluded stent, and a <t>PT2</t> <t>guidewire</t> crossed the occlusion. G) The occluded stent was dilated with a 2.0x15mm balloon, and the post-dilatation angiography displayed partial open of the stent. H) The stent was further dilated with a larger 4.0x20 balloon, and the final angiography confirmed complete patency of the stent.
    Pt2 Tetr, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pt2 tetr/product/Addgene inc
    Average 93 stars, based on 33 article reviews
    Price from $9.99 to $1999.99
    pt2 tetr - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    93
    Addgene inc pt2 hb transposon vector
    A-H) Recanalization of the chronic in-stent occlusion at the ostium of right vertebral artery. A) Posterior-anterior fluoroscopy detected a 70% stenosis at ostium of right vertebral artery. B) The stenosis was eliminated after stenting. C) A CTA performed six months after the procedure detected moderate in-stent restenosis (arrow). D) Another CTA performed 12 months after the procedure detected in-stent occlusion (arrow). E) A subsequent DSA confirmed in-stent occlusion. F) Different guidewires were attempted for passing through the occluded stent, and a <t>PT2</t> <t>guidewire</t> crossed the occlusion. G) The occluded stent was dilated with a 2.0x15mm balloon, and the post-dilatation angiography displayed partial open of the stent. H) The stent was further dilated with a larger 4.0x20 balloon, and the final angiography confirmed complete patency of the stent.
    Pt2 Hb Transposon Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pt2 hb transposon vector/product/Addgene inc
    Average 93 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    pt2 hb transposon vector - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    92
    Addgene inc pt2 shp53 gfp4 vector
    A-H) Recanalization of the chronic in-stent occlusion at the ostium of right vertebral artery. A) Posterior-anterior fluoroscopy detected a 70% stenosis at ostium of right vertebral artery. B) The stenosis was eliminated after stenting. C) A CTA performed six months after the procedure detected moderate in-stent restenosis (arrow). D) Another CTA performed 12 months after the procedure detected in-stent occlusion (arrow). E) A subsequent DSA confirmed in-stent occlusion. F) Different guidewires were attempted for passing through the occluded stent, and a <t>PT2</t> <t>guidewire</t> crossed the occlusion. G) The occluded stent was dilated with a 2.0x15mm balloon, and the post-dilatation angiography displayed partial open of the stent. H) The stent was further dilated with a larger 4.0x20 balloon, and the final angiography confirmed complete patency of the stent.
    Pt2 Shp53 Gfp4 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pt2 shp53 gfp4 vector/product/Addgene inc
    Average 92 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    pt2 shp53 gfp4 vector - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    90
    Addgene inc pt2 c fluc plasmid
    A-H) Recanalization of the chronic in-stent occlusion at the ostium of right vertebral artery. A) Posterior-anterior fluoroscopy detected a 70% stenosis at ostium of right vertebral artery. B) The stenosis was eliminated after stenting. C) A CTA performed six months after the procedure detected moderate in-stent restenosis (arrow). D) Another CTA performed 12 months after the procedure detected in-stent occlusion (arrow). E) A subsequent DSA confirmed in-stent occlusion. F) Different guidewires were attempted for passing through the occluded stent, and a <t>PT2</t> <t>guidewire</t> crossed the occlusion. G) The occluded stent was dilated with a 2.0x15mm balloon, and the post-dilatation angiography displayed partial open of the stent. H) The stent was further dilated with a larger 4.0x20 balloon, and the final angiography confirmed complete patency of the stent.
    Pt2 C Fluc Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pt2 c fluc plasmid/product/Addgene inc
    Average 90 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    pt2 c fluc plasmid - by Bioz Stars, 2020-08
    90/100 stars
      Buy from Supplier

    90
    Addgene inc pt2 vector
    Determination of integration sites in immortalized cell lines by inverse PCR. (A) Southern blot analysis of transgenic cassettes in several immortalized lines with a puromycin probe. pT1-2 and <t>pT2-1</t> denote different clones derived from transfection by
    Pt2 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pt2 vector/product/Addgene inc
    Average 90 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    pt2 vector - by Bioz Stars, 2020-08
    90/100 stars
      Buy from Supplier

    88
    Boston Scientific pt2 0 014
    Determination of integration sites in immortalized cell lines by inverse PCR. (A) Southern blot analysis of transgenic cassettes in several immortalized lines with a puromycin probe. pT1-2 and <t>pT2-1</t> denote different clones derived from transfection by
    Pt2 0 014, supplied by Boston Scientific, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pt2 0 014/product/Boston Scientific
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pt2 0 014 - by Bioz Stars, 2020-08
    88/100 stars
      Buy from Supplier

    84
    Thermo Fisher plasmid pt2
    Experimental approach. a) mouse ES cells in culture are co-transfected with a barcoded reporter library and the Sleeping Beauty 100X transposase. b) The reporters in the <t>pT2</t> transposon backbone are integrated at random in the mouse genome. c) Mismatches occur during the repair of a double-strand break induced by the transient expression of I-SceI. If the double-strand break is repaired through Non Homologous End Joining (NHEJ), no mismatch is formed. If it is repaired by Single Strand Annealing (SSA), a mismatch is formed and repaired in favor of one of the two nucleotides. Sequencing the construct reveals the outcome of DNA repair at different locations identified with the barcode.
    Plasmid Pt2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasmid pt2/product/Thermo Fisher
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    plasmid pt2 - by Bioz Stars, 2020-08
    84/100 stars
      Buy from Supplier

    91
    Addgene inc pt2 bh transposon cloning vector
    Experimental approach. a) mouse ES cells in culture are co-transfected with a barcoded reporter library and the Sleeping Beauty 100X transposase. b) The reporters in the <t>pT2</t> transposon backbone are integrated at random in the mouse genome. c) Mismatches occur during the repair of a double-strand break induced by the transient expression of I-SceI. If the double-strand break is repaired through Non Homologous End Joining (NHEJ), no mismatch is formed. If it is repaired by Single Strand Annealing (SSA), a mismatch is formed and repaired in favor of one of the two nucleotides. Sequencing the construct reveals the outcome of DNA repair at different locations identified with the barcode.
    Pt2 Bh Transposon Cloning Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pt2 bh transposon cloning vector/product/Addgene inc
    Average 91 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    pt2 bh transposon cloning vector - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

    94
    Addgene inc pt2 c luc
    Experimental approach. a) mouse ES cells in culture are co-transfected with a barcoded reporter library and the Sleeping Beauty 100X transposase. b) The reporters in the <t>pT2</t> transposon backbone are integrated at random in the mouse genome. c) Mismatches occur during the repair of a double-strand break induced by the transient expression of I-SceI. If the double-strand break is repaired through Non Homologous End Joining (NHEJ), no mismatch is formed. If it is repaired by Single Strand Annealing (SSA), a mismatch is formed and repaired in favor of one of the two nucleotides. Sequencing the construct reveals the outcome of DNA repair at different locations identified with the barcode.
    Pt2 C Luc, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pt2 c luc/product/Addgene inc
    Average 94 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    pt2 c luc - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

    85
    Lonza pt2 gfp transposon
    Efficiency of murine T lymphocyte electroporation is dependent on different buffer and mouse strain. ( A ) Total lymphocytes from lymph nodes of C57BL/6 mice were isolated and electroporated using in house buffers and 4 µg of <t>pT2-GFP</t> plasmid. Cell viability and GFP expression were analyzed after 24 h by flow cytometry. Electroporation scores were determined as described in materials and methods . Statistical analysis was performed using One Way ANOVA and Tukey post test. P
    Pt2 Gfp Transposon, supplied by Lonza, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pt2 gfp transposon/product/Lonza
    Average 85 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    pt2 gfp transposon - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

    Image Search Results


    Comparative Analysis of Transfection Efficiency and Transgenic Rate Using the pFAR4 or the pT2 Plasmid to Encode the Venus Gene HeLa cells were co-transfected with either a high or a low plasmid amount using the following constructs: pFAR4-ITRs-SV-Venus (2,984 bp, 300 or 50 ng) or an equimolar amount of pT2/SV-Venus (4,878 bp, 490 or 82 ng) either without (−SB) or plus SB100X transposase encoded by the pFAR4 plasmid (30 or 5 ng). Using the pGL3 empty vector, the total amount of plasmid was adjusted to 800 or 500 ng for the high or low conditions, respectively. (A) Data represent the mean number of fluorescent cells ± SD, obtained 2 days after six and four independent transfections for the high and low plasmid amounts, respectively. **p

    Journal: Molecular Therapy. Nucleic Acids

    Article Title: The Antibiotic-free pFAR4 Vector Paired with the Sleeping Beauty Transposon System Mediates Efficient Transgene Delivery in Human Cells

    doi: 10.1016/j.omtn.2017.12.017

    Figure Lengend Snippet: Comparative Analysis of Transfection Efficiency and Transgenic Rate Using the pFAR4 or the pT2 Plasmid to Encode the Venus Gene HeLa cells were co-transfected with either a high or a low plasmid amount using the following constructs: pFAR4-ITRs-SV-Venus (2,984 bp, 300 or 50 ng) or an equimolar amount of pT2/SV-Venus (4,878 bp, 490 or 82 ng) either without (−SB) or plus SB100X transposase encoded by the pFAR4 plasmid (30 or 5 ng). Using the pGL3 empty vector, the total amount of plasmid was adjusted to 800 or 500 ng for the high or low conditions, respectively. (A) Data represent the mean number of fluorescent cells ± SD, obtained 2 days after six and four independent transfections for the high and low plasmid amounts, respectively. **p

    Article Snippet: Plasmid Constructs and Manipulation pFAR4-ITRs is a derivative of the antibiotic-free pFAR4 vector that contains the ITR sequence elements extracted from pT2/BH (a gift from P. Hackett, Addgene plasmid 26556; Cambridge MA, USA). pFAR4-ITRs-SV-Neo (3,385 bp), pFAR4-ITRs-CAGGS-Venus (pFAR-Venus, 4,227 bp), and pFAR4-CMV-SB100X (3,094 bp) were constructed using standard cloning procedures and the following donor templates: pT2/SV-Neo (5,155 bp), pT2-CAGGS-Venus (pT2-Venus, 6,132 bp), and pCMV-CAT(T7)-SB100X (4,752 bp).

    Techniques: Transfection, Transgenic Assay, Plasmid Preparation, Construct

    Analysis of Transfection Efficiency, PEDF Expression, and Secretion by Primary Human RPE Cells 13 and 21 Days after Transfection (A) Eight individual cultures of RPE cells isolated from 4 donor eyes (age, 74.0 ± 6.0 years; gender, 2 males and 2 females; time postmortem, 26.3 ± 3.9 hr; cultivation time before transfection, 48.8 ± 10.0 days) were transfected with 30 ng pFAR4-CMV SB100X SV40 transposase and either 470 ng pT2-ITRs CAGGS Venus (0.11 pmol) or 324 ng pFAR4-ITRs CAGGS Venus (0.11 pmol + 146 ng pFAR4/empty plasmid DNA) or without plasmid DNA as a control. Venus expression for 1 × 10 5 cells was analyzed by flow cytometry at 13.0 ± 1.8 days after transfection. Statistical analysis using an unpaired two-tailed t test showed a significantly greater number of fluorescent cells when transfected with pFAR4-Venus than when transfected with pT2-Venus (p = 0.0277). However, no difference in mean fluorescence intensity was noted. (B) PEDF secretion was analyzed by western blots of culture medium of 1 × 10 4 RPE cells isolated from 10 human donor eyes (age, 68.3 ± 14.8 years; gender, 6 males and 4 females; time postmortem, 29.5 ± 19.6 hr; cultivation time before transfection, 39.3 ± 22.3 days) and transfected with equimolar concentrations of pT2-PEDF (470 ng) or pFAR4-PEDF (313 ng + 157 ng pFAR4/empty plasmid DNA) in the presence of 30 ng of SB100X carried by pFAR4 or without plasmid DNA as a control. Culture supernatants of 72 individual control cultures, 116 individual pT2-PEDF cultures, and 114 individual pFAR4-PEDF cultures were analyzed for total PEDF secretion 21.1 ± 1.1 days after transfection. Loading of equal amounts of culture supernatants was proven by SDS-PAGE Coomassie G-250 staining. Using anti-PEDF antibodies, signal intensities obtained with cells transfected with either pFAR4-PEDF or pT2-PEDF were normalized to the signal intensities obtained with control cells electroporated without plasmid DNA. Total PEDF secretion from pT2-PEDF- or pFAR4-PEDF-transfected cells was significantly higher than from cells transfected without plasmid DNA (p ≤ 0.001), and pFAR4-PEDF-transfected cells secreted a significantly higher PEDF level than pT2-PEDF-transfected cells (p ≤ 0.001, one-way ANOVA with Tukey’s multiple comparisons test). (C) Relative PEDF gene expression was analyzed by qPCR for 1 × 10 4 RPE cells isolated from 7 donor eyes (age, 63.7 ± 15.5 years; gender, 5 males and 2 females; time postmortem, 32.1 ± 23.4 hr; cultivation time before transfection, 37.6 ± 17.8 days), transfected with equimolar concentrations of pT2-PEDF and pFAR4-PEDF (using the same conditions as described above), and cultured for 21.3 ± 1.3 days after transfection. Data for total (endogenous + recombinant) PEDF gene expression are presented as a box and whisker plot (whiskers, minimum to maximum). Total PEDF gene expression in pFAR4-PEDF-transfected cells was related to that obtained with pT2-PEDF-transfected cells, which was set to 1 (dashed line). The difference is statistically significant, p = 0.0406 (one sample t test), exhibiting a 1.89-fold increase. Transfection of RPE cells with pFAR4-PEDF allowed for a significant increase in total PEDF expression (p = 0.0333, unpaired two-tailed t test) compared with the endogenous PEDF level (data not shown). (D) Total PEDF secretion for 1 × 10 4 cells transfected with equimolar concentrations of pT2-PEDF and pFAR4-PEDF (using the same conditions as described in B) was quantified by ELISA using RPE cells isolated from 9 human donor eyes (age, 67.4 ± 15.4 years; gender, 6 males and 3 females; time postmortem, 30.1 ± 20.7 hours; cultivation time before transfection, 41.6 ± 22.5 days). Culture supernatants of 36 individual control cultures, 32 individual pT2-PEDF cultures, and 32 individual pFAR4-PEDF cultures were analyzed 21.0 ± 1.1 days after transfection. Total PEDF secretion of pT2-PEDF and pFAR4-PEDF transfected cells was compared with non-transfected control cells (p = not significant for pT2-PEDF-transfected cells and p ≤ 0.001 for pFAR4-PEDF-transfected cells), and total PEDF secretion of pT2-PEDF-transfected cells was compared with total PEDF secretion of pFAR4-PEDF-transfected cells (p ≤ 0.05, one-way ANOVA with Tukey’s multiple comparisons test). All data are expressed as mean ± SD.

    Journal: Molecular Therapy. Nucleic Acids

    Article Title: The Antibiotic-free pFAR4 Vector Paired with the Sleeping Beauty Transposon System Mediates Efficient Transgene Delivery in Human Cells

    doi: 10.1016/j.omtn.2017.12.017

    Figure Lengend Snippet: Analysis of Transfection Efficiency, PEDF Expression, and Secretion by Primary Human RPE Cells 13 and 21 Days after Transfection (A) Eight individual cultures of RPE cells isolated from 4 donor eyes (age, 74.0 ± 6.0 years; gender, 2 males and 2 females; time postmortem, 26.3 ± 3.9 hr; cultivation time before transfection, 48.8 ± 10.0 days) were transfected with 30 ng pFAR4-CMV SB100X SV40 transposase and either 470 ng pT2-ITRs CAGGS Venus (0.11 pmol) or 324 ng pFAR4-ITRs CAGGS Venus (0.11 pmol + 146 ng pFAR4/empty plasmid DNA) or without plasmid DNA as a control. Venus expression for 1 × 10 5 cells was analyzed by flow cytometry at 13.0 ± 1.8 days after transfection. Statistical analysis using an unpaired two-tailed t test showed a significantly greater number of fluorescent cells when transfected with pFAR4-Venus than when transfected with pT2-Venus (p = 0.0277). However, no difference in mean fluorescence intensity was noted. (B) PEDF secretion was analyzed by western blots of culture medium of 1 × 10 4 RPE cells isolated from 10 human donor eyes (age, 68.3 ± 14.8 years; gender, 6 males and 4 females; time postmortem, 29.5 ± 19.6 hr; cultivation time before transfection, 39.3 ± 22.3 days) and transfected with equimolar concentrations of pT2-PEDF (470 ng) or pFAR4-PEDF (313 ng + 157 ng pFAR4/empty plasmid DNA) in the presence of 30 ng of SB100X carried by pFAR4 or without plasmid DNA as a control. Culture supernatants of 72 individual control cultures, 116 individual pT2-PEDF cultures, and 114 individual pFAR4-PEDF cultures were analyzed for total PEDF secretion 21.1 ± 1.1 days after transfection. Loading of equal amounts of culture supernatants was proven by SDS-PAGE Coomassie G-250 staining. Using anti-PEDF antibodies, signal intensities obtained with cells transfected with either pFAR4-PEDF or pT2-PEDF were normalized to the signal intensities obtained with control cells electroporated without plasmid DNA. Total PEDF secretion from pT2-PEDF- or pFAR4-PEDF-transfected cells was significantly higher than from cells transfected without plasmid DNA (p ≤ 0.001), and pFAR4-PEDF-transfected cells secreted a significantly higher PEDF level than pT2-PEDF-transfected cells (p ≤ 0.001, one-way ANOVA with Tukey’s multiple comparisons test). (C) Relative PEDF gene expression was analyzed by qPCR for 1 × 10 4 RPE cells isolated from 7 donor eyes (age, 63.7 ± 15.5 years; gender, 5 males and 2 females; time postmortem, 32.1 ± 23.4 hr; cultivation time before transfection, 37.6 ± 17.8 days), transfected with equimolar concentrations of pT2-PEDF and pFAR4-PEDF (using the same conditions as described above), and cultured for 21.3 ± 1.3 days after transfection. Data for total (endogenous + recombinant) PEDF gene expression are presented as a box and whisker plot (whiskers, minimum to maximum). Total PEDF gene expression in pFAR4-PEDF-transfected cells was related to that obtained with pT2-PEDF-transfected cells, which was set to 1 (dashed line). The difference is statistically significant, p = 0.0406 (one sample t test), exhibiting a 1.89-fold increase. Transfection of RPE cells with pFAR4-PEDF allowed for a significant increase in total PEDF expression (p = 0.0333, unpaired two-tailed t test) compared with the endogenous PEDF level (data not shown). (D) Total PEDF secretion for 1 × 10 4 cells transfected with equimolar concentrations of pT2-PEDF and pFAR4-PEDF (using the same conditions as described in B) was quantified by ELISA using RPE cells isolated from 9 human donor eyes (age, 67.4 ± 15.4 years; gender, 6 males and 3 females; time postmortem, 30.1 ± 20.7 hours; cultivation time before transfection, 41.6 ± 22.5 days). Culture supernatants of 36 individual control cultures, 32 individual pT2-PEDF cultures, and 32 individual pFAR4-PEDF cultures were analyzed 21.0 ± 1.1 days after transfection. Total PEDF secretion of pT2-PEDF and pFAR4-PEDF transfected cells was compared with non-transfected control cells (p = not significant for pT2-PEDF-transfected cells and p ≤ 0.001 for pFAR4-PEDF-transfected cells), and total PEDF secretion of pT2-PEDF-transfected cells was compared with total PEDF secretion of pFAR4-PEDF-transfected cells (p ≤ 0.05, one-way ANOVA with Tukey’s multiple comparisons test). All data are expressed as mean ± SD.

    Article Snippet: Plasmid Constructs and Manipulation pFAR4-ITRs is a derivative of the antibiotic-free pFAR4 vector that contains the ITR sequence elements extracted from pT2/BH (a gift from P. Hackett, Addgene plasmid 26556; Cambridge MA, USA). pFAR4-ITRs-SV-Neo (3,385 bp), pFAR4-ITRs-CAGGS-Venus (pFAR-Venus, 4,227 bp), and pFAR4-CMV-SB100X (3,094 bp) were constructed using standard cloning procedures and the following donor templates: pT2/SV-Neo (5,155 bp), pT2-CAGGS-Venus (pT2-Venus, 6,132 bp), and pCMV-CAT(T7)-SB100X (4,752 bp).

    Techniques: Transfection, Expressing, Isolation, Plasmid Preparation, Flow Cytometry, Cytometry, Two Tailed Test, Fluorescence, Western Blot, SDS Page, Staining, Real-time Polymerase Chain Reaction, Cell Culture, Recombinant, Whisker Assay, Enzyme-linked Immunosorbent Assay

    Analysis of Long-Term PEDF Expression and Secretion by Primary Human RPE Cells Transfected with SB100X Encoded by pFAR4 and PEDF Carried by Either the pFAR4 or pT2 Plasmid (A) At the times indicated in the graph, PEDF secretion was analyzed by western blots of culture media of 1 × 10 4 RPE cells isolated from human donor eyes (numbers as indicated; the number of samples tested is listed in Table S1 ) transfected with equimolar concentrations of pT2-PEDF or pFAR4-PEDF (7.50 × 10 10 plasmid copies) combined with 30 ng of SB100X encoded by the pFAR4 plasmid. The total plasmid amount (500 ng) was adjusted using pFAR4 empty vector. Signal intensities obtained with pT2-PEDF-transfected and pFAR4-PEDF-transfected cells were normalized to the signal intensities obtained with cells electroporated without plasmid DNA. Data are presented as mean ± SD. Statistical analyses were performed to compare level of PEDF secreted by cells transfected with plasmids to that of cells electroporated without plasmid and PEDF values obtained with pFAR4-PEDF-transfected cells versus those of pT2-PEDF cells using one-way ANOVA with Tukey’s multiple comparisons test. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001. See also Table S1 . (B) Relative PEDF gene expression was analyzed by qPCR in 1 × 10 4 RPE cells isolated from 8 human donor eyes (age, 66.3 ± 16.0 years; gender, 5 males and 3 females; time postmortem, 31.1 ± 21.8 hr; cultivation time before transfection, 39.9 ± 23.4 days) and cultured for 180 ± 66 days after transfection with equimolar concentrations of pT2-PEDF and pFAR4-PEDF (as described in A). Data for total (endogenous + recombinant) PEDF gene expression are presented as a box and whisker plot (whiskers, minimum to maximum). PEDF gene expression in pFAR4-PEDF-transfected cells was related to that obtained with pT2-PEDF-transfected cells, which was set to 1 (dashed line). In cells transfected with pFAR4-PEDF, the total PEDF gene expression level was 2.2-fold higher than in cells transfected with pT2-PEDF. This difference is statistically significant, with p = 0.0123, using one-sample t test.

    Journal: Molecular Therapy. Nucleic Acids

    Article Title: The Antibiotic-free pFAR4 Vector Paired with the Sleeping Beauty Transposon System Mediates Efficient Transgene Delivery in Human Cells

    doi: 10.1016/j.omtn.2017.12.017

    Figure Lengend Snippet: Analysis of Long-Term PEDF Expression and Secretion by Primary Human RPE Cells Transfected with SB100X Encoded by pFAR4 and PEDF Carried by Either the pFAR4 or pT2 Plasmid (A) At the times indicated in the graph, PEDF secretion was analyzed by western blots of culture media of 1 × 10 4 RPE cells isolated from human donor eyes (numbers as indicated; the number of samples tested is listed in Table S1 ) transfected with equimolar concentrations of pT2-PEDF or pFAR4-PEDF (7.50 × 10 10 plasmid copies) combined with 30 ng of SB100X encoded by the pFAR4 plasmid. The total plasmid amount (500 ng) was adjusted using pFAR4 empty vector. Signal intensities obtained with pT2-PEDF-transfected and pFAR4-PEDF-transfected cells were normalized to the signal intensities obtained with cells electroporated without plasmid DNA. Data are presented as mean ± SD. Statistical analyses were performed to compare level of PEDF secreted by cells transfected with plasmids to that of cells electroporated without plasmid and PEDF values obtained with pFAR4-PEDF-transfected cells versus those of pT2-PEDF cells using one-way ANOVA with Tukey’s multiple comparisons test. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001. See also Table S1 . (B) Relative PEDF gene expression was analyzed by qPCR in 1 × 10 4 RPE cells isolated from 8 human donor eyes (age, 66.3 ± 16.0 years; gender, 5 males and 3 females; time postmortem, 31.1 ± 21.8 hr; cultivation time before transfection, 39.9 ± 23.4 days) and cultured for 180 ± 66 days after transfection with equimolar concentrations of pT2-PEDF and pFAR4-PEDF (as described in A). Data for total (endogenous + recombinant) PEDF gene expression are presented as a box and whisker plot (whiskers, minimum to maximum). PEDF gene expression in pFAR4-PEDF-transfected cells was related to that obtained with pT2-PEDF-transfected cells, which was set to 1 (dashed line). In cells transfected with pFAR4-PEDF, the total PEDF gene expression level was 2.2-fold higher than in cells transfected with pT2-PEDF. This difference is statistically significant, with p = 0.0123, using one-sample t test.

    Article Snippet: Plasmid Constructs and Manipulation pFAR4-ITRs is a derivative of the antibiotic-free pFAR4 vector that contains the ITR sequence elements extracted from pT2/BH (a gift from P. Hackett, Addgene plasmid 26556; Cambridge MA, USA). pFAR4-ITRs-SV-Neo (3,385 bp), pFAR4-ITRs-CAGGS-Venus (pFAR-Venus, 4,227 bp), and pFAR4-CMV-SB100X (3,094 bp) were constructed using standard cloning procedures and the following donor templates: pT2/SV-Neo (5,155 bp), pT2-CAGGS-Venus (pT2-Venus, 6,132 bp), and pCMV-CAT(T7)-SB100X (4,752 bp).

    Techniques: Expressing, Transfection, Plasmid Preparation, Western Blot, Isolation, Real-time Polymerase Chain Reaction, Cell Culture, Recombinant, Whisker Assay

    Delivery of the Sleeping Beauty Transposon System by the pFAR4 Vector Mediates a Higher Number of Neomycin-Resistant Clones HeLa cells were co-transfected with either pT2/SV-Neo and pCMV-(CAT)T7-SB100X (ratio transposon plasmid:transposase plasmid = 500:50 ng or 50:5 ng) or an equimolar amount of pFAR4-ITRs-SV-Neo and pFAR4-CMV-SB100X (ratio transposon plasmid:transposase plasmid = 328.3:32.5 ng or 32.8:3.3 ng). The total DNA amount was adjusted to 550 ng using pFAR4 empty vector. (A) Both vectors contain identical eukaryotic expression cassettes but alternative plasmid backbones, differing by their size and the selection markers used for their propagation in E. coli . (B) Transgenic rates are the mean number of NeoR colonies after 11 days of growth in selection medium per number of cells seeded ± SD. Data are the average of three independent transfection experiments with replicated cell seedings (n = 23) for the “high” plasmid amount and four independent transfection experiments for the “low” plasmid amount (n = 40). +SB and −SB indicate the presence or absence of the transposase plasmid, respectively. **p

    Journal: Molecular Therapy. Nucleic Acids

    Article Title: The Antibiotic-free pFAR4 Vector Paired with the Sleeping Beauty Transposon System Mediates Efficient Transgene Delivery in Human Cells

    doi: 10.1016/j.omtn.2017.12.017

    Figure Lengend Snippet: Delivery of the Sleeping Beauty Transposon System by the pFAR4 Vector Mediates a Higher Number of Neomycin-Resistant Clones HeLa cells were co-transfected with either pT2/SV-Neo and pCMV-(CAT)T7-SB100X (ratio transposon plasmid:transposase plasmid = 500:50 ng or 50:5 ng) or an equimolar amount of pFAR4-ITRs-SV-Neo and pFAR4-CMV-SB100X (ratio transposon plasmid:transposase plasmid = 328.3:32.5 ng or 32.8:3.3 ng). The total DNA amount was adjusted to 550 ng using pFAR4 empty vector. (A) Both vectors contain identical eukaryotic expression cassettes but alternative plasmid backbones, differing by their size and the selection markers used for their propagation in E. coli . (B) Transgenic rates are the mean number of NeoR colonies after 11 days of growth in selection medium per number of cells seeded ± SD. Data are the average of three independent transfection experiments with replicated cell seedings (n = 23) for the “high” plasmid amount and four independent transfection experiments for the “low” plasmid amount (n = 40). +SB and −SB indicate the presence or absence of the transposase plasmid, respectively. **p

    Article Snippet: Plasmid Constructs and Manipulation pFAR4-ITRs is a derivative of the antibiotic-free pFAR4 vector that contains the ITR sequence elements extracted from pT2/BH (a gift from P. Hackett, Addgene plasmid 26556; Cambridge MA, USA). pFAR4-ITRs-SV-Neo (3,385 bp), pFAR4-ITRs-CAGGS-Venus (pFAR-Venus, 4,227 bp), and pFAR4-CMV-SB100X (3,094 bp) were constructed using standard cloning procedures and the following donor templates: pT2/SV-Neo (5,155 bp), pT2-CAGGS-Venus (pT2-Venus, 6,132 bp), and pCMV-CAT(T7)-SB100X (4,752 bp).

    Techniques: Plasmid Preparation, Clone Assay, Transfection, Chloramphenicol Acetyltransferase Assay, Expressing, Selection, Transgenic Assay

    Ratio of phosphorylated Rb (pRb) after transfecting A549 cells with plasmids encoding either CTDSP1 (A), CTDSP2 (B), or CTDSPL (C) In all three cases, the ratio of phosphorylated Rb protein is decreased after transfection. pRb/Rb, relative amount of pRb normalized to the total Rb protein. Intact A549 were compared with the slowest growing clones of A549 stably transfected with pT2/HB-СTDSP1, 2 or L. A minimum of two independent ELISA experiments were performed. The data represent the means of 8 wells ± SD. * P ≤0.05; ** P ≤0.01; *** P ≤0.001 (Mann–Whitney U-test).

    Journal: Bioscience Reports

    Article Title: Tumor suppressor properties of the small C-terminal domain phosphatases in non-small cell lung cancer

    doi: 10.1042/BSR20193094

    Figure Lengend Snippet: Ratio of phosphorylated Rb (pRb) after transfecting A549 cells with plasmids encoding either CTDSP1 (A), CTDSP2 (B), or CTDSPL (C) In all three cases, the ratio of phosphorylated Rb protein is decreased after transfection. pRb/Rb, relative amount of pRb normalized to the total Rb protein. Intact A549 were compared with the slowest growing clones of A549 stably transfected with pT2/HB-СTDSP1, 2 or L. A minimum of two independent ELISA experiments were performed. The data represent the means of 8 wells ± SD. * P ≤0.05; ** P ≤0.01; *** P ≤0.001 (Mann–Whitney U-test).

    Article Snippet: The pCMV(CAT)T7-SB100 vector was a gift from Dr. Zsuzsanna Izsvak (Addgene plasmid # 34879) and pT2/HB was a gift from Dr. Perry Hackett (Addgene plasmid # 26557).

    Techniques: Transfection, Clone Assay, Stable Transfection, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

    A-H) Recanalization of the chronic in-stent occlusion at the ostium of right vertebral artery. A) Posterior-anterior fluoroscopy detected a 70% stenosis at ostium of right vertebral artery. B) The stenosis was eliminated after stenting. C) A CTA performed six months after the procedure detected moderate in-stent restenosis (arrow). D) Another CTA performed 12 months after the procedure detected in-stent occlusion (arrow). E) A subsequent DSA confirmed in-stent occlusion. F) Different guidewires were attempted for passing through the occluded stent, and a PT2 guidewire crossed the occlusion. G) The occluded stent was dilated with a 2.0x15mm balloon, and the post-dilatation angiography displayed partial open of the stent. H) The stent was further dilated with a larger 4.0x20 balloon, and the final angiography confirmed complete patency of the stent.

    Journal: Interventional Neuroradiology

    Article Title: Successful Recanalization of a Chronic In-stent Occlusion at the Vertebral Artery Ostium

    doi:

    Figure Lengend Snippet: A-H) Recanalization of the chronic in-stent occlusion at the ostium of right vertebral artery. A) Posterior-anterior fluoroscopy detected a 70% stenosis at ostium of right vertebral artery. B) The stenosis was eliminated after stenting. C) A CTA performed six months after the procedure detected moderate in-stent restenosis (arrow). D) Another CTA performed 12 months after the procedure detected in-stent occlusion (arrow). E) A subsequent DSA confirmed in-stent occlusion. F) Different guidewires were attempted for passing through the occluded stent, and a PT2 guidewire crossed the occlusion. G) The occluded stent was dilated with a 2.0x15mm balloon, and the post-dilatation angiography displayed partial open of the stent. H) The stent was further dilated with a larger 4.0x20 balloon, and the final angiography confirmed complete patency of the stent.

    Article Snippet: Miracle 6g, Miracle 12g and Conquest guidewires (Asahi Intec, Nagoya, Japan) were tried respectively, and finally a PT2 guidewire (Boston Scientific, Miami, FL, USA) crossed the occlusion.

    Techniques:

    Determination of integration sites in immortalized cell lines by inverse PCR. (A) Southern blot analysis of transgenic cassettes in several immortalized lines with a puromycin probe. pT1-2 and pT2-1 denote different clones derived from transfection by

    Journal: Stem Cells and Development

    Article Title: Reversible Immortalization Enables Seamless Transdifferentiation of Primary Fibroblasts into Other Lineage Cells

    doi: 10.1089/scd.2016.0035

    Figure Lengend Snippet: Determination of integration sites in immortalized cell lines by inverse PCR. (A) Southern blot analysis of transgenic cassettes in several immortalized lines with a puromycin probe. pT1-2 and pT2-1 denote different clones derived from transfection by

    Article Snippet: To generate the pT2 vector, the coding fragments of hT2, SV40LT, and EGFP were released from their individual T vectors and ligated together into the Asc I and Not I sites of 5′-PTK-3′ with CAGhT1. pBabe-hygro-hTERT and pSG5-LT plasmids were from Addgene.

    Techniques: Inverse PCR, Southern Blot, Transgenic Assay, Clone Assay, Derivative Assay, Transfection

    Generation of immortalized fibroblasts by PB-mediated gene transfer. (A) Structure of the two plasmid constructs for immortalization. pT1 contains hTERT, and pT2 contains hTERT, SV40LT, and EGFP; both have puro-TK for selection. (B) Colony morphology

    Journal: Stem Cells and Development

    Article Title: Reversible Immortalization Enables Seamless Transdifferentiation of Primary Fibroblasts into Other Lineage Cells

    doi: 10.1089/scd.2016.0035

    Figure Lengend Snippet: Generation of immortalized fibroblasts by PB-mediated gene transfer. (A) Structure of the two plasmid constructs for immortalization. pT1 contains hTERT, and pT2 contains hTERT, SV40LT, and EGFP; both have puro-TK for selection. (B) Colony morphology

    Article Snippet: To generate the pT2 vector, the coding fragments of hT2, SV40LT, and EGFP were released from their individual T vectors and ligated together into the Asc I and Not I sites of 5′-PTK-3′ with CAGhT1. pBabe-hygro-hTERT and pSG5-LT plasmids were from Addgene.

    Techniques: Plasmid Preparation, Construct, Selection

    Experimental approach. a) mouse ES cells in culture are co-transfected with a barcoded reporter library and the Sleeping Beauty 100X transposase. b) The reporters in the pT2 transposon backbone are integrated at random in the mouse genome. c) Mismatches occur during the repair of a double-strand break induced by the transient expression of I-SceI. If the double-strand break is repaired through Non Homologous End Joining (NHEJ), no mismatch is formed. If it is repaired by Single Strand Annealing (SSA), a mismatch is formed and repaired in favor of one of the two nucleotides. Sequencing the construct reveals the outcome of DNA repair at different locations identified with the barcode.

    Journal: bioRxiv

    Article Title: Strand asymmetry influences mismatch repair during single-strand annealing

    doi: 10.1101/847160

    Figure Lengend Snippet: Experimental approach. a) mouse ES cells in culture are co-transfected with a barcoded reporter library and the Sleeping Beauty 100X transposase. b) The reporters in the pT2 transposon backbone are integrated at random in the mouse genome. c) Mismatches occur during the repair of a double-strand break induced by the transient expression of I-SceI. If the double-strand break is repaired through Non Homologous End Joining (NHEJ), no mismatch is formed. If it is repaired by Single Strand Annealing (SSA), a mismatch is formed and repaired in favor of one of the two nucleotides. Sequencing the construct reveals the outcome of DNA repair at different locations identified with the barcode.

    Article Snippet: FF fragments (each with a precursor for one of heteromismatches) were synthesised by Life Technologies, and cloned into plasmid pT2 using Gibson Assembly Cloning Kit (NEB, E5510S).

    Techniques: Transfection, Expressing, Non-Homologous End Joining, Sequencing, Construct

    Efficiency of murine T lymphocyte electroporation is dependent on different buffer and mouse strain. ( A ) Total lymphocytes from lymph nodes of C57BL/6 mice were isolated and electroporated using in house buffers and 4 µg of pT2-GFP plasmid. Cell viability and GFP expression were analyzed after 24 h by flow cytometry. Electroporation scores were determined as described in materials and methods . Statistical analysis was performed using One Way ANOVA and Tukey post test. P

    Journal: PLoS ONE

    Article Title: An Efficient Low Cost Method for Gene Transfer to T Lymphocytes

    doi: 10.1371/journal.pone.0060298

    Figure Lengend Snippet: Efficiency of murine T lymphocyte electroporation is dependent on different buffer and mouse strain. ( A ) Total lymphocytes from lymph nodes of C57BL/6 mice were isolated and electroporated using in house buffers and 4 µg of pT2-GFP plasmid. Cell viability and GFP expression were analyzed after 24 h by flow cytometry. Electroporation scores were determined as described in materials and methods . Statistical analysis was performed using One Way ANOVA and Tukey post test. P

    Article Snippet: To determine electroporation efficiency, cell viability and GFP expression were evaluated after transfection using 0.5 µg of SB100X transposase and 4 µg of pT2-GFP transposon (plasmid mass recommended by Lonza). demonstrates that buffers 1M, 1SM, 2M and 3P maintained the viability of the cells above 50% and achieved superior expression of GFP at day 1 after transfection (representative plots are shown in ); accordingly, high rates of expression were sustained up to 7 days with several buffers ( ).

    Techniques: Electroporation, Mouse Assay, Isolation, Plasmid Preparation, Expressing, Flow Cytometry, Cytometry

    Electroporation of the Jurkat T cell line. ( A ) Jurkat cells were electroporated with pT2-GFP in the presence of each of the 7 different buffers. Viability and GFP expression were evaluated by flow cytometry after 24 h. Cell viability is expressed as % of the control mock electroporated condition (100%). Electroporation scores were determined as described in materials and methods . Statistical analysis was performed using One Way ANOVA and Tukey post test (* = P

    Journal: PLoS ONE

    Article Title: An Efficient Low Cost Method for Gene Transfer to T Lymphocytes

    doi: 10.1371/journal.pone.0060298

    Figure Lengend Snippet: Electroporation of the Jurkat T cell line. ( A ) Jurkat cells were electroporated with pT2-GFP in the presence of each of the 7 different buffers. Viability and GFP expression were evaluated by flow cytometry after 24 h. Cell viability is expressed as % of the control mock electroporated condition (100%). Electroporation scores were determined as described in materials and methods . Statistical analysis was performed using One Way ANOVA and Tukey post test (* = P

    Article Snippet: To determine electroporation efficiency, cell viability and GFP expression were evaluated after transfection using 0.5 µg of SB100X transposase and 4 µg of pT2-GFP transposon (plasmid mass recommended by Lonza). demonstrates that buffers 1M, 1SM, 2M and 3P maintained the viability of the cells above 50% and achieved superior expression of GFP at day 1 after transfection (representative plots are shown in ); accordingly, high rates of expression were sustained up to 7 days with several buffers ( ).

    Techniques: Electroporation, Expressing, Flow Cytometry, Cytometry

    Electroporation of resting vs activated mouse primary T lymphocytes. Total lymphocytes from lymph nodes of C57BL/6 mice were isolated and either directly electroporated or activated for 24 h with anti-CD3/anti-CD28 and then electroporated. Buffer 2S and 4 µg of pT2-GFP plasmid were used. Cell viability and GFP expression were analyzed after 24 h by flow cytometry. Cell viability is normalized to control (not electroporated) cells. Data are representative of two experiments in triplicate and expressed as mean±SEM. Data were analyzed by unpaired Student t test; p

    Journal: PLoS ONE

    Article Title: An Efficient Low Cost Method for Gene Transfer to T Lymphocytes

    doi: 10.1371/journal.pone.0060298

    Figure Lengend Snippet: Electroporation of resting vs activated mouse primary T lymphocytes. Total lymphocytes from lymph nodes of C57BL/6 mice were isolated and either directly electroporated or activated for 24 h with anti-CD3/anti-CD28 and then electroporated. Buffer 2S and 4 µg of pT2-GFP plasmid were used. Cell viability and GFP expression were analyzed after 24 h by flow cytometry. Cell viability is normalized to control (not electroporated) cells. Data are representative of two experiments in triplicate and expressed as mean±SEM. Data were analyzed by unpaired Student t test; p

    Article Snippet: To determine electroporation efficiency, cell viability and GFP expression were evaluated after transfection using 0.5 µg of SB100X transposase and 4 µg of pT2-GFP transposon (plasmid mass recommended by Lonza). demonstrates that buffers 1M, 1SM, 2M and 3P maintained the viability of the cells above 50% and achieved superior expression of GFP at day 1 after transfection (representative plots are shown in ); accordingly, high rates of expression were sustained up to 7 days with several buffers ( ).

    Techniques: Electroporation, Mouse Assay, Isolation, Plasmid Preparation, Expressing, Flow Cytometry, Cytometry

    Long term expression of GFP after electroporation with buffer 1SM. PBMCs from two healthy donors were electroporated using 1SM buffer and 4 µg of pT2-GFP plasmid. After 24 h cells were activated with anti-CD3/anti-CD28 and GFP expression was evaluated for 7 days by flow cytometry.

    Journal: PLoS ONE

    Article Title: An Efficient Low Cost Method for Gene Transfer to T Lymphocytes

    doi: 10.1371/journal.pone.0060298

    Figure Lengend Snippet: Long term expression of GFP after electroporation with buffer 1SM. PBMCs from two healthy donors were electroporated using 1SM buffer and 4 µg of pT2-GFP plasmid. After 24 h cells were activated with anti-CD3/anti-CD28 and GFP expression was evaluated for 7 days by flow cytometry.

    Article Snippet: To determine electroporation efficiency, cell viability and GFP expression were evaluated after transfection using 0.5 µg of SB100X transposase and 4 µg of pT2-GFP transposon (plasmid mass recommended by Lonza). demonstrates that buffers 1M, 1SM, 2M and 3P maintained the viability of the cells above 50% and achieved superior expression of GFP at day 1 after transfection (representative plots are shown in ); accordingly, high rates of expression were sustained up to 7 days with several buffers ( ).

    Techniques: Expressing, Electroporation, Plasmid Preparation, Flow Cytometry, Cytometry

    Comparison of in house vs commercial electroporation kit. PBMCs from two healthy donors were electroporated using either 1SM buffer or Lonza buffer and 4 µg of pT2-GFP plasmid. Cell viability and GFP expression were analyzed after 24 h by flow cytometry. Cell viability was normalized to control (not electroporated) cells. Values are the average of two donors in triplicate and are expressed as mean±SEM. Data were analyzed by unpaired Student t test; p

    Journal: PLoS ONE

    Article Title: An Efficient Low Cost Method for Gene Transfer to T Lymphocytes

    doi: 10.1371/journal.pone.0060298

    Figure Lengend Snippet: Comparison of in house vs commercial electroporation kit. PBMCs from two healthy donors were electroporated using either 1SM buffer or Lonza buffer and 4 µg of pT2-GFP plasmid. Cell viability and GFP expression were analyzed after 24 h by flow cytometry. Cell viability was normalized to control (not electroporated) cells. Values are the average of two donors in triplicate and are expressed as mean±SEM. Data were analyzed by unpaired Student t test; p

    Article Snippet: To determine electroporation efficiency, cell viability and GFP expression were evaluated after transfection using 0.5 µg of SB100X transposase and 4 µg of pT2-GFP transposon (plasmid mass recommended by Lonza). demonstrates that buffers 1M, 1SM, 2M and 3P maintained the viability of the cells above 50% and achieved superior expression of GFP at day 1 after transfection (representative plots are shown in ); accordingly, high rates of expression were sustained up to 7 days with several buffers ( ).

    Techniques: Electroporation, Plasmid Preparation, Expressing, Flow Cytometry, Cytometry

    Electroporation efficiency of different buffers in primary human T lymphocytes. ( A ) PBMCs from two healthy donors were electroporated using in house buffers and 4 µg of pT2-GFP plasmid. Cell viability and GFP expression were analyzed after 24h by flow cytometry. Cell viability is normalized to control (not electroporated) cells. Electroporation scores were determined as described in materials and methods . Values are the average of two donors in triplicate and are expressed as mean±SEM. Statistical analysis was performed using One Way ANOVA and Tukey post test (* = P

    Journal: PLoS ONE

    Article Title: An Efficient Low Cost Method for Gene Transfer to T Lymphocytes

    doi: 10.1371/journal.pone.0060298

    Figure Lengend Snippet: Electroporation efficiency of different buffers in primary human T lymphocytes. ( A ) PBMCs from two healthy donors were electroporated using in house buffers and 4 µg of pT2-GFP plasmid. Cell viability and GFP expression were analyzed after 24h by flow cytometry. Cell viability is normalized to control (not electroporated) cells. Electroporation scores were determined as described in materials and methods . Values are the average of two donors in triplicate and are expressed as mean±SEM. Statistical analysis was performed using One Way ANOVA and Tukey post test (* = P

    Article Snippet: To determine electroporation efficiency, cell viability and GFP expression were evaluated after transfection using 0.5 µg of SB100X transposase and 4 µg of pT2-GFP transposon (plasmid mass recommended by Lonza). demonstrates that buffers 1M, 1SM, 2M and 3P maintained the viability of the cells above 50% and achieved superior expression of GFP at day 1 after transfection (representative plots are shown in ); accordingly, high rates of expression were sustained up to 7 days with several buffers ( ).

    Techniques: Electroporation, Plasmid Preparation, Expressing, Flow Cytometry, Cytometry

    Electroporation of transposon and transposase maintains transgene expression after cell viability recovery. PBMCs from two healthy donors were electroporated using 1SM buffer, 20 µg of pT2-GFP plasmid and 2 µg of SB100x transposase. Cell viability and GFP expression were analyzed by flow cytometry on day 1 and 9. Values are the average of two donors in triplicate and are expressed as mean±SEM.

    Journal: PLoS ONE

    Article Title: An Efficient Low Cost Method for Gene Transfer to T Lymphocytes

    doi: 10.1371/journal.pone.0060298

    Figure Lengend Snippet: Electroporation of transposon and transposase maintains transgene expression after cell viability recovery. PBMCs from two healthy donors were electroporated using 1SM buffer, 20 µg of pT2-GFP plasmid and 2 µg of SB100x transposase. Cell viability and GFP expression were analyzed by flow cytometry on day 1 and 9. Values are the average of two donors in triplicate and are expressed as mean±SEM.

    Article Snippet: To determine electroporation efficiency, cell viability and GFP expression were evaluated after transfection using 0.5 µg of SB100X transposase and 4 µg of pT2-GFP transposon (plasmid mass recommended by Lonza). demonstrates that buffers 1M, 1SM, 2M and 3P maintained the viability of the cells above 50% and achieved superior expression of GFP at day 1 after transfection (representative plots are shown in ); accordingly, high rates of expression were sustained up to 7 days with several buffers ( ).

    Techniques: Electroporation, Expressing, Plasmid Preparation, Flow Cytometry, Cytometry