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Image Search Results
Journal: Cellular and Molecular Life Sciences: CMLS
Article Title: Absence of a robust innate immune response in rat neurons facilitates persistent infection of Borna disease virus in neuronal tissue
doi: 10.1007/s00018-013-1402-5
Figure Lengend Snippet: Absence of pSTAT1 and pSTAT2 nuclear translocation in IFN-α treated rat neurons. a–d Primary dissociated neuronal cultures of rat hippocampi were treated with IFN-α (105 U/ml) for the indicated time points and for the presence of phosphorylated STAT1 (α-pSTAT1) or STAT2 (α-pSTAT1) in astrocytes (α-GFAP) or neurons (α-Map-2) Scale bars 10 μm. Lower panels indicate the fluorescence intensity along the dashed line shown in the middle panels. e, f Statistical analysis of the mean fluorescence intensity (MFI) of pSTAT1 and pSTAT2 in astrocytes (n = 30) and neurons (n = 30) treated with IFN-α (105 U/ml) for the indicated time points. g IFN-α receptor 1 (IFNAR1) surface staining (α-IFNAR1) of neurons (α-Map-2). IFNAR1 staining was carried out with non-permeabilized neurons. For subsequent staining with Map-2, cell were extensively washed, treated with detergent and subsequently stained with anti-Map-2 antibodies. Ctrl w/o 1st AB, staining of non-permeabilized neurons with secondary antibodies only. Scale bars 10 μm
Article Snippet: Phospho-STAT quantification Immunofluorescence images from neurons and astrocytes stained against pSTAT1 or
Techniques: Translocation Assay, Fluorescence, Staining