Journal: Advanced Science

Article Title: LncRNA ARGI Contributes to Virus‐Induced Pancreatic β Cell Inflammation Through Transcriptional Activation of IFN‐Stimulated Genes

doi: 10.1002/advs.202300063

Figure Lengend Snippet: ARGI upregulation in pancreatic β cells induces an inflammatory and antiviral gene signature. A) EndoC‐ β H1 cells were transfected with an empty overexpression plasmid (pCMV6) or with a plasmid overexpressing ARGI (pARGI). ARGI expression was determined by qPCR and normalized to the reference gene β ‐actin. Results are means±SEM of 3 independent experiments; *** p < 0.001; Student's t ‐test. B) Gene Ontology analysis showed enrichment of inflammatory and antiviral pathways in ARGI ‐overexpressing pancreatic β cells. C) Scatterplot showing differentially expressed genes (black dots) in ARGI ‐overexpressing EndoC‐ β H1 cells (pARGI) compared to pCMV6‐transfected control cells. Expression values are presented as Log 10 (counts/10 6 counts). ARGI and genes from the IDIN network are indicated. D) EndoC‐ β H1 cells were transfected with a control empty plasmid (pCMV6) or with an ARGI ‐overexpressing plasmid (pARGI). Expression of MX1 , ISG15 , IFI6 , IFIT1 , IFIT3 , and STAT1 genes was determined by qPCR and normalized to the reference gene β ‐actin. Results are means ± SEM of three independent experiments; ** p < 0.01 and * p < 0.05; Student's t ‐test.

Article Snippet: The overexpression vector for ARGI harboring the T1D risk allele (ARGI‐R) was purchased from ProteoGenix (Schiltigheim, France).

Techniques: Transfection, Over Expression, Plasmid Preparation, Expressing

Journal: Structure(London, England:1993)

Article Title: How insulin-like growth factor I binds to a hybrid insulin receptor type 1 insulin-like growth factor receptor

doi: 10.1016/j.str.2022.05.007

Figure Lengend Snippet:

Article Snippet: pEE14 vector , Lonza , N/A.

Techniques: Recombinant, Sequencing, Transfection, Clone Assay, Plasmid Preparation, Software