Journal: PeerJ

Article Title: A journey through the Corynebacterium pseudotuberculosis proteome promotes insights into its functional genome

doi: 10.7717/peerj.12456

Figure Lengend Snippet: List of the studies that contributed to characterization of the C. pseudotuberculosis proteome.

Article Snippet: SDS-PAGE and immunoblot , ATCC 19410_ ovis , ATCC 809_ ovis , ATCC 43927_ ovis , ATCC 43926_ ovis , ATCC 43924_ equi , ATCC 43925_ equi , ATCC PVAMU101_ equi , Extracellular proteins and Whole cells extract , Description of protein band patterns produced by various C. pseudotuberculosis strains. In addition to the recognition of protein by antibodies of naturally infected goats. , .

Techniques: Western Blot, Produced, Infection, Labeling, Membrane

Journal: PLoS ONE

Article Title: Functional and Structural Analysis of a Highly-Expressed Yersinia pestis Small RNA following Infection of Cultured Macrophages

doi: 10.1371/journal.pone.0168915

Figure Lengend Snippet: (A) Total RNA was isolated from wild-type (WT) and knock-down (KD) strains of Ysr170 in Y . pestis and Y . pseudotuberculosis , and Ysr170 expression was analyzed by northern blot. (B) Growth curves of Y . pestis (black lines) and Y . pseudotuberculosis (red lines) wild-type (solid) and Ysr170 KD (red) strains were examined in response to different stress conditions up to 8 hrs at 2 hr intervals. Stress conditions include temperature (37°C), mild acidity (pH 5.5), host serum (10% FBS), iron starvation (100μM 2,2’-dipyridyl), and oxidative stress (1mM H 2 O 2 ). A representative experiment from three independent experiments is shown.

Article Snippet: We used the following bacterial strains: (1) pathogenic Y . pestis CO92 (pCD1+), (2) attenuated Y . pestis CO92 (pCD1-), and (3) pathogenic Y . pseudotuberculosis (ATCC 4284).

Techniques: Isolation, Knockdown, Expressing, Northern Blot

Journal: PLoS ONE

Article Title: Functional and Structural Analysis of a Highly-Expressed Yersinia pestis Small RNA following Infection of Cultured Macrophages

doi: 10.1371/journal.pone.0168915

Figure Lengend Snippet: (A) THP-1 cells were infected with Yersinia wild-type or KD Ysr170 strains at MOI 5 for 30 min to allow for pathogen invasion. Serial dilutions of THP-1 lysates (1:100) were spread on BHI agar plates, and colonies were counted after 48 hrs. The average mean and standard deviation from three representative experiments are shown. The ‘*’ denotes statistical significance (p<0.05) for number of colonies in the Ysr170 KD strains compared to wild-type (WT). (B) THP-1 cells were infected at MOI 5 with Y . pestis and Y . pseudotuberculosis wild-type or KD Ysr170 strains. After 24 hrs, cell supernatants were collected and analyzed by ELISA. The average mean and standard deviation from three representative experiments are shown. The ‘*’ denotes statistical significance (p<0.05) for TNF-α release in the Ysr170 KD strains compared to wild-type (WT). (C) THP-1 cells were infected at MOI 5 with Y . pestis pCD1+ and pCD1- strains that expressed wild-type or KD Ysr170. After 24 hrs, cell supernatants were collected and analyzed by ELISA. The average mean and standard deviation from three representative experiments are shown. The ‘*’ denotes statistical significance (p<0.05) for TNF-α release in the Ysr170 KD Y . pestis pCD1+ strains compared to wild-type (WT). (D) THP-1 cells were infected at MOI 4 with Y . pestis pCD1+ and pCD1- strains that expressed wild-type or KD Ysr170. After 24 hrs, total RNA was extracted and RT-PCR analysis was performed using specific probes to IL-8 and EGR1. The relative RNA levels are presented as fold expression in the KD Ysr170 strains compared to wild-type. For each sample, the RNA levels were normalized to 5S rRNA. The ‘*’ denotes statistical significance (p<0.05) for fold RNA change in IL-8 and EGR1 expression between pCD1+ and pCD1- strains. The average mean and standard deviation from three representative experiments are shown.

Article Snippet: We used the following bacterial strains: (1) pathogenic Y . pestis CO92 (pCD1+), (2) attenuated Y . pestis CO92 (pCD1-), and (3) pathogenic Y . pseudotuberculosis (ATCC 4284).

Techniques: Infection, Standard Deviation, Enzyme-linked Immunosorbent Assay, Reverse Transcription Polymerase Chain Reaction, Expressing