psb Search Results


antagonist psb 1115  (Tocris)
99
Tocris antagonist psb 1115
Antagonist Psb 1115, supplied by Tocris, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
antagonist psb 1115 - by Bioz Stars, 2026-06
99/100 stars
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a2b  (Tocris)
94
Tocris a2b
Figure 3. CD73þ gdT cells are the predominant Tregs in breast cancer and exhibit direct immunosuppression via the adenosine-mediated pathway. A and B, Sorted CD73þ gdT cells, CD4þ Tregs (CD45þCD3þCD4þCD25þCD127low), CD4þCD73þ and CD8þCD73þ T cells, and CD73 gdT cells (1.0 104 cells, respectively) from tumor tissues and CD73þ gdT cells (1.0 104) from paired normal tissue were cocultured with CFSE-labeled allogeneic CD4þ T cells (5.0 104) in the presence of anti-CD3 and anti- CD28. n ¼ 5. A, CD4þ T-cell proliferation was evaluated on day 6 by flow cytometry. B, Bar diagram summarizes the percentages of proliferated CD4þ T cells (CFSElow). Data, mean SEM. n ¼ 5; , P < 0.01; , P < 0.001; paired Student t test for the analysis between CD73þ gdT cells from tumor and paired normal tissue and unpaired Student t test for the analysis among other cells from tumor tissue. C, Sorted CD73þ gdT, CD4þ Tregs, CD4þCD73þ and CD8þCD73þ T cells, and CD73 gdT cells (1.0 104 cells, respectively) from tumor tissues and CD73þ gdT cells (1.0 104) from paired normal tissue were cocultured with allogeneic CD8þ T cells (5.0 104) in the presence of anti-CD3 and anti-CD28. Concentrations of perforin in the supernatants were detected on day 6 by ELISA. Data, mean SEM. n ¼ 5; , P < 0.05; , P < 0.001; paired Student t test for the analysis between CD73þ gdT cells from tumor and paired normal tissue and unpaired Student t test for the analysis among other cells from tumor tissue. D and E, Sorted CD73þ gdT cells (1.0 104) from tumor tissues were cocultured with CFSE-labeled allogeneic CD4þ T cells (5.0 104) in the presence of anti-CD3 and anti-CD28 and pretreated with anti-CD73, an antibody cocktail, control antibody, or A2A (SCH58261) and <t>A2B</t> <t>(PSB603)</t> adenosine receptor antagonists. n ¼ 5. D, CD4þ T-cell proliferation was evaluated on day 6 by flow cytometry. n ¼ 5. E, Bar diagram summarizes the percentages of proliferated CD4þ T cells (CFSElow). Data, mean SEM. n ¼ 5; ns, no significance; , P <0.001;unpaired Student t test.mAb cocktail, the mixture of antibodies for LAG-3, CTLA-4, IL10, and TGFb; N, normal tissue; T, tumor tissue.
A2b, supplied by Tocris, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
a2b - by Bioz Stars, 2026-06
94/100 stars
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2 3 6 7 tetrahydro 2 6 dioxo 1 propyl 1h purin 8 yl benzenesulphonic acid potassium salt psb1115 tocris 2009  (Tocris)
93
Tocris 2 3 6 7 tetrahydro 2 6 dioxo 1 propyl 1h purin 8 yl benzenesulphonic acid potassium salt psb1115 tocris 2009
Figure 3. CD73þ gdT cells are the predominant Tregs in breast cancer and exhibit direct immunosuppression via the adenosine-mediated pathway. A and B, Sorted CD73þ gdT cells, CD4þ Tregs (CD45þCD3þCD4þCD25þCD127low), CD4þCD73þ and CD8þCD73þ T cells, and CD73 gdT cells (1.0 104 cells, respectively) from tumor tissues and CD73þ gdT cells (1.0 104) from paired normal tissue were cocultured with CFSE-labeled allogeneic CD4þ T cells (5.0 104) in the presence of anti-CD3 and anti- CD28. n ¼ 5. A, CD4þ T-cell proliferation was evaluated on day 6 by flow cytometry. B, Bar diagram summarizes the percentages of proliferated CD4þ T cells (CFSElow). Data, mean SEM. n ¼ 5; , P < 0.01; , P < 0.001; paired Student t test for the analysis between CD73þ gdT cells from tumor and paired normal tissue and unpaired Student t test for the analysis among other cells from tumor tissue. C, Sorted CD73þ gdT, CD4þ Tregs, CD4þCD73þ and CD8þCD73þ T cells, and CD73 gdT cells (1.0 104 cells, respectively) from tumor tissues and CD73þ gdT cells (1.0 104) from paired normal tissue were cocultured with allogeneic CD8þ T cells (5.0 104) in the presence of anti-CD3 and anti-CD28. Concentrations of perforin in the supernatants were detected on day 6 by ELISA. Data, mean SEM. n ¼ 5; , P < 0.05; , P < 0.001; paired Student t test for the analysis between CD73þ gdT cells from tumor and paired normal tissue and unpaired Student t test for the analysis among other cells from tumor tissue. D and E, Sorted CD73þ gdT cells (1.0 104) from tumor tissues were cocultured with CFSE-labeled allogeneic CD4þ T cells (5.0 104) in the presence of anti-CD3 and anti-CD28 and pretreated with anti-CD73, an antibody cocktail, control antibody, or A2A (SCH58261) and <t>A2B</t> <t>(PSB603)</t> adenosine receptor antagonists. n ¼ 5. D, CD4þ T-cell proliferation was evaluated on day 6 by flow cytometry. n ¼ 5. E, Bar diagram summarizes the percentages of proliferated CD4þ T cells (CFSElow). Data, mean SEM. n ¼ 5; ns, no significance; , P <0.001;unpaired Student t test.mAb cocktail, the mixture of antibodies for LAG-3, CTLA-4, IL10, and TGFb; N, normal tissue; T, tumor tissue.
2 3 6 7 Tetrahydro 2 6 Dioxo 1 Propyl 1h Purin 8 Yl Benzenesulphonic Acid Potassium Salt Psb1115 Tocris 2009, supplied by Tocris, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
2 3 6 7 tetrahydro 2 6 dioxo 1 propyl 1h purin 8 yl benzenesulphonic acid potassium salt psb1115 tocris 2009 - by Bioz Stars, 2026-06
93/100 stars
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psb 0739  (Tocris)
95
Tocris psb 0739
Figure 3. CD73þ gdT cells are the predominant Tregs in breast cancer and exhibit direct immunosuppression via the adenosine-mediated pathway. A and B, Sorted CD73þ gdT cells, CD4þ Tregs (CD45þCD3þCD4þCD25þCD127low), CD4þCD73þ and CD8þCD73þ T cells, and CD73 gdT cells (1.0 104 cells, respectively) from tumor tissues and CD73þ gdT cells (1.0 104) from paired normal tissue were cocultured with CFSE-labeled allogeneic CD4þ T cells (5.0 104) in the presence of anti-CD3 and anti- CD28. n ¼ 5. A, CD4þ T-cell proliferation was evaluated on day 6 by flow cytometry. B, Bar diagram summarizes the percentages of proliferated CD4þ T cells (CFSElow). Data, mean SEM. n ¼ 5; , P < 0.01; , P < 0.001; paired Student t test for the analysis between CD73þ gdT cells from tumor and paired normal tissue and unpaired Student t test for the analysis among other cells from tumor tissue. C, Sorted CD73þ gdT, CD4þ Tregs, CD4þCD73þ and CD8þCD73þ T cells, and CD73 gdT cells (1.0 104 cells, respectively) from tumor tissues and CD73þ gdT cells (1.0 104) from paired normal tissue were cocultured with allogeneic CD8þ T cells (5.0 104) in the presence of anti-CD3 and anti-CD28. Concentrations of perforin in the supernatants were detected on day 6 by ELISA. Data, mean SEM. n ¼ 5; , P < 0.05; , P < 0.001; paired Student t test for the analysis between CD73þ gdT cells from tumor and paired normal tissue and unpaired Student t test for the analysis among other cells from tumor tissue. D and E, Sorted CD73þ gdT cells (1.0 104) from tumor tissues were cocultured with CFSE-labeled allogeneic CD4þ T cells (5.0 104) in the presence of anti-CD3 and anti-CD28 and pretreated with anti-CD73, an antibody cocktail, control antibody, or A2A (SCH58261) and <t>A2B</t> <t>(PSB603)</t> adenosine receptor antagonists. n ¼ 5. D, CD4þ T-cell proliferation was evaluated on day 6 by flow cytometry. n ¼ 5. E, Bar diagram summarizes the percentages of proliferated CD4þ T cells (CFSElow). Data, mean SEM. n ¼ 5; ns, no significance; , P <0.001;unpaired Student t test.mAb cocktail, the mixture of antibodies for LAG-3, CTLA-4, IL10, and TGFb; N, normal tissue; T, tumor tissue.
Psb 0739, supplied by Tocris, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 1 article reviews
psb 0739 - by Bioz Stars, 2026-06
95/100 stars
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chlorophenyl piperazide 1 sulfonyl phenyl 1 propylxanthine psb 603  (Tocris)
95
Tocris chlorophenyl piperazide 1 sulfonyl phenyl 1 propylxanthine psb 603
Figure 3. CD73þ gdT cells are the predominant Tregs in breast cancer and exhibit direct immunosuppression via the adenosine-mediated pathway. A and B, Sorted CD73þ gdT cells, CD4þ Tregs (CD45þCD3þCD4þCD25þCD127low), CD4þCD73þ and CD8þCD73þ T cells, and CD73 gdT cells (1.0 104 cells, respectively) from tumor tissues and CD73þ gdT cells (1.0 104) from paired normal tissue were cocultured with CFSE-labeled allogeneic CD4þ T cells (5.0 104) in the presence of anti-CD3 and anti- CD28. n ¼ 5. A, CD4þ T-cell proliferation was evaluated on day 6 by flow cytometry. B, Bar diagram summarizes the percentages of proliferated CD4þ T cells (CFSElow). Data, mean SEM. n ¼ 5; , P < 0.01; , P < 0.001; paired Student t test for the analysis between CD73þ gdT cells from tumor and paired normal tissue and unpaired Student t test for the analysis among other cells from tumor tissue. C, Sorted CD73þ gdT, CD4þ Tregs, CD4þCD73þ and CD8þCD73þ T cells, and CD73 gdT cells (1.0 104 cells, respectively) from tumor tissues and CD73þ gdT cells (1.0 104) from paired normal tissue were cocultured with allogeneic CD8þ T cells (5.0 104) in the presence of anti-CD3 and anti-CD28. Concentrations of perforin in the supernatants were detected on day 6 by ELISA. Data, mean SEM. n ¼ 5; , P < 0.05; , P < 0.001; paired Student t test for the analysis between CD73þ gdT cells from tumor and paired normal tissue and unpaired Student t test for the analysis among other cells from tumor tissue. D and E, Sorted CD73þ gdT cells (1.0 104) from tumor tissues were cocultured with CFSE-labeled allogeneic CD4þ T cells (5.0 104) in the presence of anti-CD3 and anti-CD28 and pretreated with anti-CD73, an antibody cocktail, control antibody, or A2A (SCH58261) and <t>A2B</t> <t>(PSB603)</t> adenosine receptor antagonists. n ¼ 5. D, CD4þ T-cell proliferation was evaluated on day 6 by flow cytometry. n ¼ 5. E, Bar diagram summarizes the percentages of proliferated CD4þ T cells (CFSElow). Data, mean SEM. n ¼ 5; ns, no significance; , P <0.001;unpaired Student t test.mAb cocktail, the mixture of antibodies for LAG-3, CTLA-4, IL10, and TGFb; N, normal tissue; T, tumor tissue.
Chlorophenyl Piperazide 1 Sulfonyl Phenyl 1 Propylxanthine Psb 603, supplied by Tocris, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/chlorophenyl piperazide 1 sulfonyl phenyl 1 propylxanthine psb 603/product/Tocris
Average 95 stars, based on 1 article reviews
chlorophenyl piperazide 1 sulfonyl phenyl 1 propylxanthine psb 603 - by Bioz Stars, 2026-06
95/100 stars
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buffer psb  (MACHEREY NAGEL)
93
MACHEREY NAGEL buffer psb
Figure 3. CD73þ gdT cells are the predominant Tregs in breast cancer and exhibit direct immunosuppression via the adenosine-mediated pathway. A and B, Sorted CD73þ gdT cells, CD4þ Tregs (CD45þCD3þCD4þCD25þCD127low), CD4þCD73þ and CD8þCD73þ T cells, and CD73 gdT cells (1.0 104 cells, respectively) from tumor tissues and CD73þ gdT cells (1.0 104) from paired normal tissue were cocultured with CFSE-labeled allogeneic CD4þ T cells (5.0 104) in the presence of anti-CD3 and anti- CD28. n ¼ 5. A, CD4þ T-cell proliferation was evaluated on day 6 by flow cytometry. B, Bar diagram summarizes the percentages of proliferated CD4þ T cells (CFSElow). Data, mean SEM. n ¼ 5; , P < 0.01; , P < 0.001; paired Student t test for the analysis between CD73þ gdT cells from tumor and paired normal tissue and unpaired Student t test for the analysis among other cells from tumor tissue. C, Sorted CD73þ gdT, CD4þ Tregs, CD4þCD73þ and CD8þCD73þ T cells, and CD73 gdT cells (1.0 104 cells, respectively) from tumor tissues and CD73þ gdT cells (1.0 104) from paired normal tissue were cocultured with allogeneic CD8þ T cells (5.0 104) in the presence of anti-CD3 and anti-CD28. Concentrations of perforin in the supernatants were detected on day 6 by ELISA. Data, mean SEM. n ¼ 5; , P < 0.05; , P < 0.001; paired Student t test for the analysis between CD73þ gdT cells from tumor and paired normal tissue and unpaired Student t test for the analysis among other cells from tumor tissue. D and E, Sorted CD73þ gdT cells (1.0 104) from tumor tissues were cocultured with CFSE-labeled allogeneic CD4þ T cells (5.0 104) in the presence of anti-CD3 and anti-CD28 and pretreated with anti-CD73, an antibody cocktail, control antibody, or A2A (SCH58261) and <t>A2B</t> <t>(PSB603)</t> adenosine receptor antagonists. n ¼ 5. D, CD4þ T-cell proliferation was evaluated on day 6 by flow cytometry. n ¼ 5. E, Bar diagram summarizes the percentages of proliferated CD4þ T cells (CFSElow). Data, mean SEM. n ¼ 5; ns, no significance; , P <0.001;unpaired Student t test.mAb cocktail, the mixture of antibodies for LAG-3, CTLA-4, IL10, and TGFb; N, normal tissue; T, tumor tissue.
Buffer Psb, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
buffer psb - by Bioz Stars, 2026-06
93/100 stars
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solubilization buffer  (Bio-Rad)
93
Bio-Rad solubilization buffer
Figure 3. CD73þ gdT cells are the predominant Tregs in breast cancer and exhibit direct immunosuppression via the adenosine-mediated pathway. A and B, Sorted CD73þ gdT cells, CD4þ Tregs (CD45þCD3þCD4þCD25þCD127low), CD4þCD73þ and CD8þCD73þ T cells, and CD73 gdT cells (1.0 104 cells, respectively) from tumor tissues and CD73þ gdT cells (1.0 104) from paired normal tissue were cocultured with CFSE-labeled allogeneic CD4þ T cells (5.0 104) in the presence of anti-CD3 and anti- CD28. n ¼ 5. A, CD4þ T-cell proliferation was evaluated on day 6 by flow cytometry. B, Bar diagram summarizes the percentages of proliferated CD4þ T cells (CFSElow). Data, mean SEM. n ¼ 5; , P < 0.01; , P < 0.001; paired Student t test for the analysis between CD73þ gdT cells from tumor and paired normal tissue and unpaired Student t test for the analysis among other cells from tumor tissue. C, Sorted CD73þ gdT, CD4þ Tregs, CD4þCD73þ and CD8þCD73þ T cells, and CD73 gdT cells (1.0 104 cells, respectively) from tumor tissues and CD73þ gdT cells (1.0 104) from paired normal tissue were cocultured with allogeneic CD8þ T cells (5.0 104) in the presence of anti-CD3 and anti-CD28. Concentrations of perforin in the supernatants were detected on day 6 by ELISA. Data, mean SEM. n ¼ 5; , P < 0.05; , P < 0.001; paired Student t test for the analysis between CD73þ gdT cells from tumor and paired normal tissue and unpaired Student t test for the analysis among other cells from tumor tissue. D and E, Sorted CD73þ gdT cells (1.0 104) from tumor tissues were cocultured with CFSE-labeled allogeneic CD4þ T cells (5.0 104) in the presence of anti-CD3 and anti-CD28 and pretreated with anti-CD73, an antibody cocktail, control antibody, or A2A (SCH58261) and <t>A2B</t> <t>(PSB603)</t> adenosine receptor antagonists. n ¼ 5. D, CD4þ T-cell proliferation was evaluated on day 6 by flow cytometry. n ¼ 5. E, Bar diagram summarizes the percentages of proliferated CD4þ T cells (CFSElow). Data, mean SEM. n ¼ 5; ns, no significance; , P <0.001;unpaired Student t test.mAb cocktail, the mixture of antibodies for LAG-3, CTLA-4, IL10, and TGFb; N, normal tissue; T, tumor tissue.
Solubilization Buffer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
solubilization buffer - by Bioz Stars, 2026-06
93/100 stars
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psb 1114  (Tocris)
92
Tocris psb 1114
Figure 3. CD73þ gdT cells are the predominant Tregs in breast cancer and exhibit direct immunosuppression via the adenosine-mediated pathway. A and B, Sorted CD73þ gdT cells, CD4þ Tregs (CD45þCD3þCD4þCD25þCD127low), CD4þCD73þ and CD8þCD73þ T cells, and CD73 gdT cells (1.0 104 cells, respectively) from tumor tissues and CD73þ gdT cells (1.0 104) from paired normal tissue were cocultured with CFSE-labeled allogeneic CD4þ T cells (5.0 104) in the presence of anti-CD3 and anti- CD28. n ¼ 5. A, CD4þ T-cell proliferation was evaluated on day 6 by flow cytometry. B, Bar diagram summarizes the percentages of proliferated CD4þ T cells (CFSElow). Data, mean SEM. n ¼ 5; , P < 0.01; , P < 0.001; paired Student t test for the analysis between CD73þ gdT cells from tumor and paired normal tissue and unpaired Student t test for the analysis among other cells from tumor tissue. C, Sorted CD73þ gdT, CD4þ Tregs, CD4þCD73þ and CD8þCD73þ T cells, and CD73 gdT cells (1.0 104 cells, respectively) from tumor tissues and CD73þ gdT cells (1.0 104) from paired normal tissue were cocultured with allogeneic CD8þ T cells (5.0 104) in the presence of anti-CD3 and anti-CD28. Concentrations of perforin in the supernatants were detected on day 6 by ELISA. Data, mean SEM. n ¼ 5; , P < 0.05; , P < 0.001; paired Student t test for the analysis between CD73þ gdT cells from tumor and paired normal tissue and unpaired Student t test for the analysis among other cells from tumor tissue. D and E, Sorted CD73þ gdT cells (1.0 104) from tumor tissues were cocultured with CFSE-labeled allogeneic CD4þ T cells (5.0 104) in the presence of anti-CD3 and anti-CD28 and pretreated with anti-CD73, an antibody cocktail, control antibody, or A2A (SCH58261) and <t>A2B</t> <t>(PSB603)</t> adenosine receptor antagonists. n ¼ 5. D, CD4þ T-cell proliferation was evaluated on day 6 by flow cytometry. n ¼ 5. E, Bar diagram summarizes the percentages of proliferated CD4þ T cells (CFSElow). Data, mean SEM. n ¼ 5; ns, no significance; , P <0.001;unpaired Student t test.mAb cocktail, the mixture of antibodies for LAG-3, CTLA-4, IL10, and TGFb; N, normal tissue; T, tumor tissue.
Psb 1114, supplied by Tocris, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 92 stars, based on 1 article reviews
psb 1114 - by Bioz Stars, 2026-06
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n 6 benzyl α β methyleneadenosine 5 diphosphate disodium salt  (Tocris)
94
Tocris n 6 benzyl α β methyleneadenosine 5 diphosphate disodium salt
Figure 3. CD73þ gdT cells are the predominant Tregs in breast cancer and exhibit direct immunosuppression via the adenosine-mediated pathway. A and B, Sorted CD73þ gdT cells, CD4þ Tregs (CD45þCD3þCD4þCD25þCD127low), CD4þCD73þ and CD8þCD73þ T cells, and CD73 gdT cells (1.0 104 cells, respectively) from tumor tissues and CD73þ gdT cells (1.0 104) from paired normal tissue were cocultured with CFSE-labeled allogeneic CD4þ T cells (5.0 104) in the presence of anti-CD3 and anti- CD28. n ¼ 5. A, CD4þ T-cell proliferation was evaluated on day 6 by flow cytometry. B, Bar diagram summarizes the percentages of proliferated CD4þ T cells (CFSElow). Data, mean SEM. n ¼ 5; , P < 0.01; , P < 0.001; paired Student t test for the analysis between CD73þ gdT cells from tumor and paired normal tissue and unpaired Student t test for the analysis among other cells from tumor tissue. C, Sorted CD73þ gdT, CD4þ Tregs, CD4þCD73þ and CD8þCD73þ T cells, and CD73 gdT cells (1.0 104 cells, respectively) from tumor tissues and CD73þ gdT cells (1.0 104) from paired normal tissue were cocultured with allogeneic CD8þ T cells (5.0 104) in the presence of anti-CD3 and anti-CD28. Concentrations of perforin in the supernatants were detected on day 6 by ELISA. Data, mean SEM. n ¼ 5; , P < 0.05; , P < 0.001; paired Student t test for the analysis between CD73þ gdT cells from tumor and paired normal tissue and unpaired Student t test for the analysis among other cells from tumor tissue. D and E, Sorted CD73þ gdT cells (1.0 104) from tumor tissues were cocultured with CFSE-labeled allogeneic CD4þ T cells (5.0 104) in the presence of anti-CD3 and anti-CD28 and pretreated with anti-CD73, an antibody cocktail, control antibody, or A2A (SCH58261) and <t>A2B</t> <t>(PSB603)</t> adenosine receptor antagonists. n ¼ 5. D, CD4þ T-cell proliferation was evaluated on day 6 by flow cytometry. n ¼ 5. E, Bar diagram summarizes the percentages of proliferated CD4þ T cells (CFSElow). Data, mean SEM. n ¼ 5; ns, no significance; , P <0.001;unpaired Student t test.mAb cocktail, the mixture of antibodies for LAG-3, CTLA-4, IL10, and TGFb; N, normal tissue; T, tumor tissue.
N 6 Benzyl α β Methyleneadenosine 5 Diphosphate Disodium Salt, supplied by Tocris, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/n 6 benzyl α β methyleneadenosine 5 diphosphate disodium salt/product/Tocris
Average 94 stars, based on 1 article reviews
n 6 benzyl α β methyleneadenosine 5 diphosphate disodium salt - by Bioz Stars, 2026-06
94/100 stars
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psb 12379  (Tocris)
94
Tocris psb 12379
Figure 3. CD73þ gdT cells are the predominant Tregs in breast cancer and exhibit direct immunosuppression via the adenosine-mediated pathway. A and B, Sorted CD73þ gdT cells, CD4þ Tregs (CD45þCD3þCD4þCD25þCD127low), CD4þCD73þ and CD8þCD73þ T cells, and CD73 gdT cells (1.0 104 cells, respectively) from tumor tissues and CD73þ gdT cells (1.0 104) from paired normal tissue were cocultured with CFSE-labeled allogeneic CD4þ T cells (5.0 104) in the presence of anti-CD3 and anti- CD28. n ¼ 5. A, CD4þ T-cell proliferation was evaluated on day 6 by flow cytometry. B, Bar diagram summarizes the percentages of proliferated CD4þ T cells (CFSElow). Data, mean SEM. n ¼ 5; , P < 0.01; , P < 0.001; paired Student t test for the analysis between CD73þ gdT cells from tumor and paired normal tissue and unpaired Student t test for the analysis among other cells from tumor tissue. C, Sorted CD73þ gdT, CD4þ Tregs, CD4þCD73þ and CD8þCD73þ T cells, and CD73 gdT cells (1.0 104 cells, respectively) from tumor tissues and CD73þ gdT cells (1.0 104) from paired normal tissue were cocultured with allogeneic CD8þ T cells (5.0 104) in the presence of anti-CD3 and anti-CD28. Concentrations of perforin in the supernatants were detected on day 6 by ELISA. Data, mean SEM. n ¼ 5; , P < 0.05; , P < 0.001; paired Student t test for the analysis between CD73þ gdT cells from tumor and paired normal tissue and unpaired Student t test for the analysis among other cells from tumor tissue. D and E, Sorted CD73þ gdT cells (1.0 104) from tumor tissues were cocultured with CFSE-labeled allogeneic CD4þ T cells (5.0 104) in the presence of anti-CD3 and anti-CD28 and pretreated with anti-CD73, an antibody cocktail, control antibody, or A2A (SCH58261) and <t>A2B</t> <t>(PSB603)</t> adenosine receptor antagonists. n ¼ 5. D, CD4þ T-cell proliferation was evaluated on day 6 by flow cytometry. n ¼ 5. E, Bar diagram summarizes the percentages of proliferated CD4þ T cells (CFSElow). Data, mean SEM. n ¼ 5; ns, no significance; , P <0.001;unpaired Student t test.mAb cocktail, the mixture of antibodies for LAG-3, CTLA-4, IL10, and TGFb; N, normal tissue; T, tumor tissue.
Psb 12379, supplied by Tocris, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/psb 12379/product/Tocris
Average 94 stars, based on 1 article reviews
psb 12379 - by Bioz Stars, 2026-06
94/100 stars
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psb cb5  (Tocris)
94
Tocris psb cb5
The effect of receptor blockers on the effect of AEA’s combination with LPI on breast cancer cell lines’ viability. The following substances and concentrations were used: CB1, SR 141716A (100 nM); CB2, SR 144528 (100 nM); GPR55, ML-193 (2 µM); GPR18, <t>PSB</t> <t>CB5</t> (3 µM). Incubation time: 72 h; resazurin test, mean ± standard error ( n = 4 experiments). ( A ) MCF-10A, ( B ) MCF-7, ( C ) BT-474, ( D ) SK-BR-3, ( E ) BT-20, ( F ) MDA-MB-231.
Psb Cb5, supplied by Tocris, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
psb cb5 - by Bioz Stars, 2026-06
94/100 stars
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Figure 3. CD73þ gdT cells are the predominant Tregs in breast cancer and exhibit direct immunosuppression via the adenosine-mediated pathway. A and B, Sorted CD73þ gdT cells, CD4þ Tregs (CD45þCD3þCD4þCD25þCD127low), CD4þCD73þ and CD8þCD73þ T cells, and CD73 gdT cells (1.0 104 cells, respectively) from tumor tissues and CD73þ gdT cells (1.0 104) from paired normal tissue were cocultured with CFSE-labeled allogeneic CD4þ T cells (5.0 104) in the presence of anti-CD3 and anti- CD28. n ¼ 5. A, CD4þ T-cell proliferation was evaluated on day 6 by flow cytometry. B, Bar diagram summarizes the percentages of proliferated CD4þ T cells (CFSElow). Data, mean SEM. n ¼ 5; , P < 0.01; , P < 0.001; paired Student t test for the analysis between CD73þ gdT cells from tumor and paired normal tissue and unpaired Student t test for the analysis among other cells from tumor tissue. C, Sorted CD73þ gdT, CD4þ Tregs, CD4þCD73þ and CD8þCD73þ T cells, and CD73 gdT cells (1.0 104 cells, respectively) from tumor tissues and CD73þ gdT cells (1.0 104) from paired normal tissue were cocultured with allogeneic CD8þ T cells (5.0 104) in the presence of anti-CD3 and anti-CD28. Concentrations of perforin in the supernatants were detected on day 6 by ELISA. Data, mean SEM. n ¼ 5; , P < 0.05; , P < 0.001; paired Student t test for the analysis between CD73þ gdT cells from tumor and paired normal tissue and unpaired Student t test for the analysis among other cells from tumor tissue. D and E, Sorted CD73þ gdT cells (1.0 104) from tumor tissues were cocultured with CFSE-labeled allogeneic CD4þ T cells (5.0 104) in the presence of anti-CD3 and anti-CD28 and pretreated with anti-CD73, an antibody cocktail, control antibody, or A2A (SCH58261) and A2B (PSB603) adenosine receptor antagonists. n ¼ 5. D, CD4þ T-cell proliferation was evaluated on day 6 by flow cytometry. n ¼ 5. E, Bar diagram summarizes the percentages of proliferated CD4þ T cells (CFSElow). Data, mean SEM. n ¼ 5; ns, no significance; , P <0.001;unpaired Student t test.mAb cocktail, the mixture of antibodies for LAG-3, CTLA-4, IL10, and TGFb; N, normal tissue; T, tumor tissue.

Journal: Cancer Immunology Research

Article Title: An IL6–Adenosine Positive Feedback Loop between CD73+ γδTregs and CAFs Promotes Tumor Progression in Human Breast Cancer

doi: 10.1158/2326-6066.cir-19-0923

Figure Lengend Snippet: Figure 3. CD73þ gdT cells are the predominant Tregs in breast cancer and exhibit direct immunosuppression via the adenosine-mediated pathway. A and B, Sorted CD73þ gdT cells, CD4þ Tregs (CD45þCD3þCD4þCD25þCD127low), CD4þCD73þ and CD8þCD73þ T cells, and CD73 gdT cells (1.0 104 cells, respectively) from tumor tissues and CD73þ gdT cells (1.0 104) from paired normal tissue were cocultured with CFSE-labeled allogeneic CD4þ T cells (5.0 104) in the presence of anti-CD3 and anti- CD28. n ¼ 5. A, CD4þ T-cell proliferation was evaluated on day 6 by flow cytometry. B, Bar diagram summarizes the percentages of proliferated CD4þ T cells (CFSElow). Data, mean SEM. n ¼ 5; , P < 0.01; , P < 0.001; paired Student t test for the analysis between CD73þ gdT cells from tumor and paired normal tissue and unpaired Student t test for the analysis among other cells from tumor tissue. C, Sorted CD73þ gdT, CD4þ Tregs, CD4þCD73þ and CD8þCD73þ T cells, and CD73 gdT cells (1.0 104 cells, respectively) from tumor tissues and CD73þ gdT cells (1.0 104) from paired normal tissue were cocultured with allogeneic CD8þ T cells (5.0 104) in the presence of anti-CD3 and anti-CD28. Concentrations of perforin in the supernatants were detected on day 6 by ELISA. Data, mean SEM. n ¼ 5; , P < 0.05; , P < 0.001; paired Student t test for the analysis between CD73þ gdT cells from tumor and paired normal tissue and unpaired Student t test for the analysis among other cells from tumor tissue. D and E, Sorted CD73þ gdT cells (1.0 104) from tumor tissues were cocultured with CFSE-labeled allogeneic CD4þ T cells (5.0 104) in the presence of anti-CD3 and anti-CD28 and pretreated with anti-CD73, an antibody cocktail, control antibody, or A2A (SCH58261) and A2B (PSB603) adenosine receptor antagonists. n ¼ 5. D, CD4þ T-cell proliferation was evaluated on day 6 by flow cytometry. n ¼ 5. E, Bar diagram summarizes the percentages of proliferated CD4þ T cells (CFSElow). Data, mean SEM. n ¼ 5; ns, no significance; , P <0.001;unpaired Student t test.mAb cocktail, the mixture of antibodies for LAG-3, CTLA-4, IL10, and TGFb; N, normal tissue; T, tumor tissue.

Article Snippet: A1 (KW3902, CAS No. 136199-02-5, 0.05 mmol/L, Tocris), A3 (MRS1220, CAS No. 183721-15-5, 0.05 mmol/L, Tocris), A2A (SCH58261, CAS No. 160098-96-4, 0.1 mmol/L, Tocris), and A2B (PSB603, CAS No. 1092351-10-4, 0.05 mmol/L, Tocris) adenosine receptor antagonists; p38 MAPK (SB203580, CAS No. 152121-47-6, 0.05 mmol/L, Abmole), ERK1/2 (SCH772984, CAS No. 942183-80-4, 0.01 mmol/L, Abmole); or JNK (SP600125, CAS No. 129-56-6, 0.05 mmol/L, Abmole) inhibitors; and anti-CD73 were added to some cultures.

Techniques: Labeling, Cytometry, Enzyme-linked Immunosorbent Assay, Control

Figure 6. CD73þ gdTregs upregulate IL6 expression in CAFs via the adenosine/A2BR/p38MAPK pathway. A and B, Sorted CD73þ gdT cells from tumor tissue were cocultured with CAFs (5.0 104) in different E:T ratios (0:1, 0.1:1, 0.2:1, 0.5:1, or 1:1) in the presence of anti-CD3 and anti-CD28 for 6 days. n ¼ 5; unpaired Student t test. A, The relative mRNA expression of IL6 in CAFs was determined by RT-PCR and normalized to GAPDH. n ¼ 5; unpaired Student t test. B, Concentrations of IL6 in supernatants were detected by ELISAs. Data, mean SEM. n ¼ 5; ns, no significance; , P < 0.01; unpaired Student t test. C–E, Sorted CD73þ gdT cells from tumor tissues were cocultured with CAFs (5.0 104) in different E:T ratios (0:1, 0.2:1, or 0.5:1) in the presence of anti-CD3 and anti-CD28 for 6 days.The expression of p38 (C), ERK1/2 (D), and JNK (E) in CAFs was analyzed by flow cytometry. Bar diagrams summarize the percentages of p38, ERK1/2, and JNK in CAFs. Data, mean SEM. n ¼ 5; ns, no significance; , P < 0.01; unpaired Student t test. F and G, Sorted CD73þ gdT cells (2.5 104) from tumor tissues were cocultured with CAFs (5.0 104) in different conditions [medium, A1 (KW3902), A3 (MRS1220), A2A (SCH58261), and A2B (PSB603) adenosine receptor antagonists, p38 MAPK (SB203580), ERK1/2 (SCH772984), or JNK (SP600125) inhibitor, anti-CD73 or control antibody] in the presence of anti-CD3 and anti-CD28 for 6 days. F, The relative mRNA expression of IL6 in CAFs was determined by RT-PCR and normalized to GAPDH. G, Concentrations of IL6 in supernatants were detected by ELISAs. Data, mean SEM. n ¼ 5; ns, no significance; , P < 0.01.

Journal: Cancer Immunology Research

Article Title: An IL6–Adenosine Positive Feedback Loop between CD73+ γδTregs and CAFs Promotes Tumor Progression in Human Breast Cancer

doi: 10.1158/2326-6066.cir-19-0923

Figure Lengend Snippet: Figure 6. CD73þ gdTregs upregulate IL6 expression in CAFs via the adenosine/A2BR/p38MAPK pathway. A and B, Sorted CD73þ gdT cells from tumor tissue were cocultured with CAFs (5.0 104) in different E:T ratios (0:1, 0.1:1, 0.2:1, 0.5:1, or 1:1) in the presence of anti-CD3 and anti-CD28 for 6 days. n ¼ 5; unpaired Student t test. A, The relative mRNA expression of IL6 in CAFs was determined by RT-PCR and normalized to GAPDH. n ¼ 5; unpaired Student t test. B, Concentrations of IL6 in supernatants were detected by ELISAs. Data, mean SEM. n ¼ 5; ns, no significance; , P < 0.01; unpaired Student t test. C–E, Sorted CD73þ gdT cells from tumor tissues were cocultured with CAFs (5.0 104) in different E:T ratios (0:1, 0.2:1, or 0.5:1) in the presence of anti-CD3 and anti-CD28 for 6 days.The expression of p38 (C), ERK1/2 (D), and JNK (E) in CAFs was analyzed by flow cytometry. Bar diagrams summarize the percentages of p38, ERK1/2, and JNK in CAFs. Data, mean SEM. n ¼ 5; ns, no significance; , P < 0.01; unpaired Student t test. F and G, Sorted CD73þ gdT cells (2.5 104) from tumor tissues were cocultured with CAFs (5.0 104) in different conditions [medium, A1 (KW3902), A3 (MRS1220), A2A (SCH58261), and A2B (PSB603) adenosine receptor antagonists, p38 MAPK (SB203580), ERK1/2 (SCH772984), or JNK (SP600125) inhibitor, anti-CD73 or control antibody] in the presence of anti-CD3 and anti-CD28 for 6 days. F, The relative mRNA expression of IL6 in CAFs was determined by RT-PCR and normalized to GAPDH. G, Concentrations of IL6 in supernatants were detected by ELISAs. Data, mean SEM. n ¼ 5; ns, no significance; , P < 0.01.

Article Snippet: A1 (KW3902, CAS No. 136199-02-5, 0.05 mmol/L, Tocris), A3 (MRS1220, CAS No. 183721-15-5, 0.05 mmol/L, Tocris), A2A (SCH58261, CAS No. 160098-96-4, 0.1 mmol/L, Tocris), and A2B (PSB603, CAS No. 1092351-10-4, 0.05 mmol/L, Tocris) adenosine receptor antagonists; p38 MAPK (SB203580, CAS No. 152121-47-6, 0.05 mmol/L, Abmole), ERK1/2 (SCH772984, CAS No. 942183-80-4, 0.01 mmol/L, Abmole); or JNK (SP600125, CAS No. 129-56-6, 0.05 mmol/L, Abmole) inhibitors; and anti-CD73 were added to some cultures.

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Cytometry, Control

The effect of receptor blockers on the effect of AEA’s combination with LPI on breast cancer cell lines’ viability. The following substances and concentrations were used: CB1, SR 141716A (100 nM); CB2, SR 144528 (100 nM); GPR55, ML-193 (2 µM); GPR18, PSB CB5 (3 µM). Incubation time: 72 h; resazurin test, mean ± standard error ( n = 4 experiments). ( A ) MCF-10A, ( B ) MCF-7, ( C ) BT-474, ( D ) SK-BR-3, ( E ) BT-20, ( F ) MDA-MB-231.

Journal: International Journal of Molecular Sciences

Article Title: The Interaction of the Endocannabinoid Anandamide and Paracannabinoid Lysophosphatidylinositol during Cell Death Induction in Human Breast Cancer Cells

doi: 10.3390/ijms25042271

Figure Lengend Snippet: The effect of receptor blockers on the effect of AEA’s combination with LPI on breast cancer cell lines’ viability. The following substances and concentrations were used: CB1, SR 141716A (100 nM); CB2, SR 144528 (100 nM); GPR55, ML-193 (2 µM); GPR18, PSB CB5 (3 µM). Incubation time: 72 h; resazurin test, mean ± standard error ( n = 4 experiments). ( A ) MCF-10A, ( B ) MCF-7, ( C ) BT-474, ( D ) SK-BR-3, ( E ) BT-20, ( F ) MDA-MB-231.

Article Snippet: SR 144028, PSB CB5, ML-184, ML-193, and LPI were from Tocris Bioscience, Bristol, UK.

Techniques: Incubation