prs314 Search Results


plasmid prs314  (ATCC)
91
ATCC plasmid prs314
Comparison of relative PDI and intracellular β-glucosidase levels during β-glucosidase expression in different yeast strains after exponential growth phase (48 h) in batch culture normalized to wild-type β-glucosidase in BJ5464. 1 OD-mL yeast cells expressing β-glucosidase in 25 mL batch culture were collected and lysed as described in Materials and Methods. Cell lysates were subjected to SDS-PAGE and the intracellular PDI was detected by Western blotting. The PDI levels relative to the parent strain <t> BJ5464+pRS314-β </t> were determined using ImageQuant software (Molecular Dynamics). The range of PDI levels is based on five sets of Western blot data.
Plasmid Prs314, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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alexa fluortm 568  (Addgene inc)
93
Addgene inc alexa fluortm 568
Comparison of relative PDI and intracellular β-glucosidase levels during β-glucosidase expression in different yeast strains after exponential growth phase (48 h) in batch culture normalized to wild-type β-glucosidase in BJ5464. 1 OD-mL yeast cells expressing β-glucosidase in 25 mL batch culture were collected and lysed as described in Materials and Methods. Cell lysates were subjected to SDS-PAGE and the intracellular PDI was detected by Western blotting. The PDI levels relative to the parent strain <t> BJ5464+pRS314-β </t> were determined using ImageQuant software (Molecular Dynamics). The range of PDI levels is based on five sets of Western blot data.
Alexa Fluortm 568, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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position 13 11  (Addgene inc)
91
Addgene inc position 13 11
Comparison of relative PDI and intracellular β-glucosidase levels during β-glucosidase expression in different yeast strains after exponential growth phase (48 h) in batch culture normalized to wild-type β-glucosidase in BJ5464. 1 OD-mL yeast cells expressing β-glucosidase in 25 mL batch culture were collected and lysed as described in Materials and Methods. Cell lysates were subjected to SDS-PAGE and the intracellular PDI was detected by Western blotting. The PDI levels relative to the parent strain <t> BJ5464+pRS314-β </t> were determined using ImageQuant software (Molecular Dynamics). The range of PDI levels is based on five sets of Western blot data.
Position 13 11, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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a 11006  (Addgene inc)
90
Addgene inc a 11006
Comparison of relative PDI and intracellular β-glucosidase levels during β-glucosidase expression in different yeast strains after exponential growth phase (48 h) in batch culture normalized to wild-type β-glucosidase in BJ5464. 1 OD-mL yeast cells expressing β-glucosidase in 25 mL batch culture were collected and lysed as described in Materials and Methods. Cell lysates were subjected to SDS-PAGE and the intracellular PDI was detected by Western blotting. The PDI levels relative to the parent strain <t> BJ5464+pRS314-β </t> were determined using ImageQuant software (Molecular Dynamics). The range of PDI levels is based on five sets of Western blot data.
A 11006, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mdm2 plasmids  (Addgene inc)
91
Addgene inc mdm2 plasmids
Comparison of relative PDI and intracellular β-glucosidase levels during β-glucosidase expression in different yeast strains after exponential growth phase (48 h) in batch culture normalized to wild-type β-glucosidase in BJ5464. 1 OD-mL yeast cells expressing β-glucosidase in 25 mL batch culture were collected and lysed as described in Materials and Methods. Cell lysates were subjected to SDS-PAGE and the intracellular PDI was detected by Western blotting. The PDI levels relative to the parent strain <t> BJ5464+pRS314-β </t> were determined using ImageQuant software (Molecular Dynamics). The range of PDI levels is based on five sets of Western blot data.
Mdm2 Plasmids, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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prs314  (Addgene inc)
85
Addgene inc prs314
Comparison of relative PDI and intracellular β-glucosidase levels during β-glucosidase expression in different yeast strains after exponential growth phase (48 h) in batch culture normalized to wild-type β-glucosidase in BJ5464. 1 OD-mL yeast cells expressing β-glucosidase in 25 mL batch culture were collected and lysed as described in Materials and Methods. Cell lysates were subjected to SDS-PAGE and the intracellular PDI was detected by Western blotting. The PDI levels relative to the parent strain <t> BJ5464+pRS314-β </t> were determined using ImageQuant software (Molecular Dynamics). The range of PDI levels is based on five sets of Western blot data.
Prs314, supplied by Addgene inc, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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yos9-proteina-his7 fusion protein  (PROTEINA Co Ltd)
90
PROTEINA Co Ltd yos9-proteina-his7 fusion protein
Comparison of relative PDI and intracellular β-glucosidase levels during β-glucosidase expression in different yeast strains after exponential growth phase (48 h) in batch culture normalized to wild-type β-glucosidase in BJ5464. 1 OD-mL yeast cells expressing β-glucosidase in 25 mL batch culture were collected and lysed as described in Materials and Methods. Cell lysates were subjected to SDS-PAGE and the intracellular PDI was detected by Western blotting. The PDI levels relative to the parent strain <t> BJ5464+pRS314-β </t> were determined using ImageQuant software (Molecular Dynamics). The range of PDI levels is based on five sets of Western blot data.
Yos9 Proteina His7 Fusion Protein, supplied by PROTEINA Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plasmid prs314-hht2-hhf220  (Purdue University Cytometry)
90
Purdue University Cytometry plasmid prs314-hht2-hhf220
Comparison of relative PDI and intracellular β-glucosidase levels during β-glucosidase expression in different yeast strains after exponential growth phase (48 h) in batch culture normalized to wild-type β-glucosidase in BJ5464. 1 OD-mL yeast cells expressing β-glucosidase in 25 mL batch culture were collected and lysed as described in Materials and Methods. Cell lysates were subjected to SDS-PAGE and the intracellular PDI was detected by Western blotting. The PDI levels relative to the parent strain <t> BJ5464+pRS314-β </t> were determined using ImageQuant software (Molecular Dynamics). The range of PDI levels is based on five sets of Western blot data.
Plasmid Prs314 Hht2 Hhf220, supplied by Purdue University Cytometry, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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prs314- tbf1- δ i  (Gilson Inc)
90
Gilson Inc prs314- tbf1- δ i
Comparison of relative PDI and intracellular β-glucosidase levels during β-glucosidase expression in different yeast strains after exponential growth phase (48 h) in batch culture normalized to wild-type β-glucosidase in BJ5464. 1 OD-mL yeast cells expressing β-glucosidase in 25 mL batch culture were collected and lysed as described in Materials and Methods. Cell lysates were subjected to SDS-PAGE and the intracellular PDI was detected by Western blotting. The PDI levels relative to the parent strain <t> BJ5464+pRS314-β </t> were determined using ImageQuant software (Molecular Dynamics). The range of PDI levels is based on five sets of Western blot data.
Prs314 Tbf1 δ I, supplied by Gilson Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plasmid prs314-rlm1-10m-3ha  (GenScript corporation)
90
GenScript corporation plasmid prs314-rlm1-10m-3ha
Comparison of relative PDI and intracellular β-glucosidase levels during β-glucosidase expression in different yeast strains after exponential growth phase (48 h) in batch culture normalized to wild-type β-glucosidase in BJ5464. 1 OD-mL yeast cells expressing β-glucosidase in 25 mL batch culture were collected and lysed as described in Materials and Methods. Cell lysates were subjected to SDS-PAGE and the intracellular PDI was detected by Western blotting. The PDI levels relative to the parent strain <t> BJ5464+pRS314-β </t> were determined using ImageQuant software (Molecular Dynamics). The range of PDI levels is based on five sets of Western blot data.
Plasmid Prs314 Rlm1 10m 3ha, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cen-trp1 plasmid (prs314) containing + nbp1/ + ylr458w  (Promega)
90
Promega cen-trp1 plasmid (prs314) containing + nbp1/ + ylr458w
Comparison of relative PDI and intracellular β-glucosidase levels during β-glucosidase expression in different yeast strains after exponential growth phase (48 h) in batch culture normalized to wild-type β-glucosidase in BJ5464. 1 OD-mL yeast cells expressing β-glucosidase in 25 mL batch culture were collected and lysed as described in Materials and Methods. Cell lysates were subjected to SDS-PAGE and the intracellular PDI was detected by Western blotting. The PDI levels relative to the parent strain <t> BJ5464+pRS314-β </t> were determined using ImageQuant software (Molecular Dynamics). The range of PDI levels is based on five sets of Western blot data.
Cen Trp1 Plasmid (Prs314) Containing + Nbp1/ + Ylr458w, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plasmid prs314-x123 psxyl2 encoding xylitol dehydrogenase (psxdhp)  (Haiying Enterprise Group Co Ltd)
90
Haiying Enterprise Group Co Ltd plasmid prs314-x123 psxyl2 encoding xylitol dehydrogenase (psxdhp)
Comparison of relative PDI and intracellular β-glucosidase levels during β-glucosidase expression in different yeast strains after exponential growth phase (48 h) in batch culture normalized to wild-type β-glucosidase in BJ5464. 1 OD-mL yeast cells expressing β-glucosidase in 25 mL batch culture were collected and lysed as described in Materials and Methods. Cell lysates were subjected to SDS-PAGE and the intracellular PDI was detected by Western blotting. The PDI levels relative to the parent strain <t> BJ5464+pRS314-β </t> were determined using ImageQuant software (Molecular Dynamics). The range of PDI levels is based on five sets of Western blot data.
Plasmid Prs314 X123 Psxyl2 Encoding Xylitol Dehydrogenase (Psxdhp), supplied by Haiying Enterprise Group Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Comparison of relative PDI and intracellular β-glucosidase levels during β-glucosidase expression in different yeast strains after exponential growth phase (48 h) in batch culture normalized to wild-type β-glucosidase in BJ5464. 1 OD-mL yeast cells expressing β-glucosidase in 25 mL batch culture were collected and lysed as described in Materials and Methods. Cell lysates were subjected to SDS-PAGE and the intracellular PDI was detected by Western blotting. The PDI levels relative to the parent strain BJ5464+pRS314-β were determined using ImageQuant software (Molecular Dynamics). The range of PDI levels is based on five sets of Western blot data.

Journal:

Article Title: PDI Improves Secretion of Redox-Inactive β-glucosidase

doi: 10.1021/bp060287p

Figure Lengend Snippet: Comparison of relative PDI and intracellular β-glucosidase levels during β-glucosidase expression in different yeast strains after exponential growth phase (48 h) in batch culture normalized to wild-type β-glucosidase in BJ5464. 1 OD-mL yeast cells expressing β-glucosidase in 25 mL batch culture were collected and lysed as described in Materials and Methods. Cell lysates were subjected to SDS-PAGE and the intracellular PDI was detected by Western blotting. The PDI levels relative to the parent strain BJ5464+pRS314-β were determined using ImageQuant software (Molecular Dynamics). The range of PDI levels is based on five sets of Western blot data.

Article Snippet: Plasmids The single copy expression plasmid pRS314-β was constructed from a secretion-modified full-length β-glucosidase with an N-terminal synthetic pre-pro secretion signal sequence and a 10-amino-acid C-terminal c- myc detection tag under the control of a GAL1-10 promoter and the plasmid pRS314 (ATCC) containing the TRP1 marker as reported previously 5 .

Techniques: Comparison, Expressing, Western Blot, Software